Rapid and efficient genetic transformation method for agrobacterium tumefaciens-mediated wheat stem tips

Abstract

The invention discloses a rapid and efficient genetic transformation method for agrobacterium tumefaciens-mediated wheat stem tips. The method mainly comprises the steps of carrying out water seed soaking, accelerating germination and cold treatment on mature wheat seeds, transversely cutting coleoptiles of the wheat seeds until exposing stem tips, taking the cut wheat seeds as a transformation acceptor material, carrying out genetic transformation by virtue of vacuum treatment mediation on agrobacterium tumefaciens, placing the transformed material in a suitable growth environment, and carrying out transgenic identification after the plants grow, so as to obtain transgenic wheat plants. By virtue of the rapid and efficient genetic transformation method, the culture of callus tissues of the wheat is omitted, so that the influences caused by various factors to the genetic transformation of the wheat are reduced, and the problems of vitrification, browning, cell mutation and the like in the tissue culture process are avoided; the genetic transformation conditions of the wheat are optimized, so that the economic cost of the genetic transformation of the wheat is lowered, the restriction of the genotype of the wheat to the transformation efficiency is effectively overcome, and a large amount of transgenic wheat plants can be rapidly obtained; and the rapid and efficient genetic transformation method has significant values to the culture of high-quality new-variety wheat and the research of the genetic transformation of the wheat.

Description

technical field [0001] The invention belongs to the fields of agricultural biotechnology and plant genetic engineering, in particular to a fast and efficient method for genetic transformation of wheat mediated by Agrobacterium. Background technique [0002] As one of the most important food crops in the world, wheat is one of the main sources of protein and calories for human beings. According to the prediction of the International Food Policy Research Institute, the demand for wheat in the world will grow at an annual rate of 1.6%. Urgent problems. The traditional wheat hybrid breeding process is slow and has the disadvantages of gene linkage and reproductive isolation, making it difficult to produce new quality wheat. Mutation breeding with the help of some physical and chemical conditions requires a large amount of materials, and it is difficult to control the direction of mutagenesis, the beneficial mutation rate is low, and it is difficult to screen. Obtaining hybrid...

AI Technical Summary

Problems solved by technology

Studies have shown that traditional wheat Agrobacterium genetic transformation often requires the use of plant tissue culture techniques, and the wheat receptor genotype is one of the important factors affecting the transformation efficiency of Agrobacterium. Different wheat receptor genotypes have different sensitivities to Agrobacterium. The transformation efficiency is also significantly different. Studies have shown that only some wheat genotypes have high regeneration frequency of acceptor materials, and most of the wheat with important economic value have large genotype differences and poor regeneration performance, so it is still difficult to transform. Therefore, It is of great significance to establish a wheat genetic transformation method that is less dependent on the genotype of the transformed receptor

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