9 results about "Coleoptile" patented technology

The invention discloses a salt-stress coleoptile elongation QTL (quantitative trait loci) related molecular marker located on a wheat 3B chromosome and application of the molecular marker. The invention provides application of single nucleotide polymorphism of a specific SNP site on a wheat genome as a marker in identification or auxiliary identification of the coleoptile length of wheat under salt stress. The specific SNP site is located at the 36th site of SEQ ID NO: 4 on the wheat 3B chromosome, and the nucleotide at the SNP site is A or G. The method is of great significance to cultivation of salt-tolerant wheat varieties.

The invention discloses an application of a regulatory gene FgCTF1 beta 1 for synthesizing deoxynivalenol from fusarium graminearum. The regulatory gene FgCTF1 beta 1 is used for synthesizing deoxynivalenol from fusarium graminearum. According to the invention, a gene engineering strain delta FgCTF1 beta 1 is obtained by knocking out an FgCTF1 beta 1 gene in a fusarium graminearum genome. Compared with a wild strain PH-1, the genetic engineering strain delta FgCTF1 beta 1 has no significant change in the aspects of mycelial growth, conidium generation, pathogenicity to wheat coleoptiles and the like, but the biosynthesis amount of deoxynivalenol is significantly reduced. The invention also provides application of the FgCTF1 beta 1 gene as an inhibition target or a knockout target in inhibition of synthesis of DON from fusarium graminearum, and provides a new strategy and technical approach for prevention and control of pollution of deoxynivalenol in agricultural products.

The invention discloses a non-tissue-culture-dependent pearl millet injection transformation method, and belongs to the technical field of plant genetic engineering. The method comprises the following steps: selecting germinated seeds of pearl foxtail millet with the primary bud length of 0.8-1.0 cm as receptors; preparing agrobacterium liquid, and adjusting the concentration to OD600 = 0.5; injecting the bacterial liquid into the embryo along the coleoptile direction of the seed until the bacterial liquid seeps from the end part of the coleoptile; co-culturing the transformed receptor for 24 hours under a dark condition after injection, and transplanting the transformed receptor into a seedling culture pot for cultivation; and finally, carrying out PCR (Polymerase Chain Reaction) screening. By optimizing key parameters such as the receptor development stage, the agrobacterium strain type and the bacterial liquid concentration, efficient genetic transformation of the pearl millet without tissue culture is achieved, and the T0 transformation positive rate reaches 63%. According to the invention, the transformation process is simplified, the transformation period is shortened, the cost is reduced, the transformation efficiency and stability are improved, and an effective technical support is provided for gene function research and molecular breeding of the pearl millet.

The invention discloses a rice seed germination detection method based on image recognition, and belongs to the field of image recognition, and the method comprises the steps: obtaining an original image sequence of a rice seed germination process based on a rice seed sample, and carrying out the preprocessing of the sequence, and obtaining an enhanced image sequence; processing the enhanced image sequence by adopting an image segmentation algorithm and an edge detection technology in sequence to obtain an independent feature map of a multi-layer structure; rice germination features are extracted from the independent feature map, the rice germination features comprise the coleoptile cracking degree, the endosperm density change and the total root length and the total root number, and the rice germination features are integrated into a comprehensive dynamic vector through a time sequence fusion method; calculating a consumption rate based on a coleoptile cracking degree and endosperm density change to obtain a correlation index of surface layer and middle layer change; and carrying out classification processing on the correlation indexes by adopting a convolutional neural network, judging the synchronism of multi-level change by integrating the total length and the total root number of the root system, and determining the overall health degree score of the germination state.

The invention discloses a salt-stress coleoptile elongation QTL (quantitative trait loci) related molecular marker located on a wheat 6B chromosome and application of the molecular marker. The invention provides application of single nucleotide polymorphism of a specific SNP site on a wheat genome as a marker in identification or auxiliary identification of the coleoptile length of wheat under salt stress. The specific SNP site is located at the 35th site of SEQ ID NO: 4 on a wheat 6B chromosome, and the nucleotide at the SNP site is T or C. The method is of great significance to cultivation of salt-tolerant wheat varieties.

The present disclosure relates in general to the imbibition of grains for the agronomic, horticulture, animal feed, and malting industries. More specifically, the present disclosure relates to systems and methods for improving germination and plant seedling development rates using low volts of direct current to increase the productivity, efficiency, and economics of industries that rely on grain germination and seedling development to produce a product. The application of less than or equal to approximately 36.0 volts of direct current to grains in aqueous solutions for at least approximately thirty minutes has been found to significantly improve coleoptile length, radicle length, invitro neutral detergent fiber digestibility, germination percentage, glucose concentration, and amino acid concentration on a dry matter basis.

The present disclosure relates in general to the imbibition of grains for the agronomic, horticulture, animal feed, and malting industries. More specifically, the present disclosure relates to systems and methods for improving germination and plant seedling development rates using low volts of direct current to increase the productivity, efficiency, and economics of industries that rely on grain germination and seedling development to produce a product. The application of less than or equal to approximately 36.0 volts of direct current to grains in aqueous solutions for at least approximately thirty minutes has been found to significantly improve coleoptile length, radicle length, invitro neutral detergent fiber digestibility, germination percentage, glucose concentration, and amino acid concentration on a dry matter basis.

This invention relates to the field of bioengineering, and discloses... OsDS1 Application of genes in the creation of water-resistant, direct-seeding rice germplasm using genetic engineering. This invention is the first to discover the gene encoding the initiation enzyme of the rice shikimic acid synthesis pathway. OsDS1 This is related to the waterlogging tolerance and direct seeding suitability of rice. Based on this, the present invention utilizes... OsDS1 The target gene is used to increase the expression level of the gene through transgenic or gene editing methods to create new rice germplasm. This type of germplasm has the characteristic of rapid coleoptile elongation after germination under flooded or non-flooded conditions, thereby improving the direct seeding suitability of rice.

The present application relates to the technical field of wheat coleoptile length measurement in simulated soil test, and discloses a wheat coleoptile length measurement device in simulated soil environment for salt tolerance test, which comprises a device main body, the device main body comprises a simulated cultivation pool at the middle position and liquid supplement tanks on both sides of the simulated cultivation pool, the inner walls of the liquid supplement tanks and the simulated cultivation pool are uniformly provided with partition plates, and the bottoms of the liquid supplement tanks on both sides of the simulated cultivation pool are fixedly connected with cavities. The present application simulates the soil environment by setting multiple layers of simulated filter paper soaked with wheat culture solution, and realizes real-time supplement of the wheat culture solution on the multiple layers of simulated filter paper through the liquid supplement opening. The growth state of the wheat seeds in the salt tolerance state is recorded in an undamaged state by clamping and fixing filter paper clamping frames on the periphery of the multiple layers of simulated filter paper. The device is more suitable for taking out and observing the growth state of the wheat by additionally arranging rubber plate plugs and push plates at the liquid supplement opening.