Method for human relay working of endogenetic epiphyte in natural rice straw into grassplot grass

Abstract

A method for artificially transplanting endophytic fungi from the natural grass to the lawn grass comprises the steps as follows: inoculating the seeds which are determined to be aseptic onto the surface of a water-agar culture medium under the aseptic condition, and slightly pressing; sealing the culture dish, placing the culture dish into a constant-temperature incubator, and carrying out the dark culture to obtain aseptic coleoptile; longitudinally cutting a small opening at the upper part of the coleoptile with a depth that the coleoptiles is not penetrated, injecting mycelia into the opening, inoculating, placing into a constant-temperature incubator and carrying out the dark culture for 5 days; culturing in the dark and in the light alternately for 4 days, transplanting into the aseptic vermiculite, sealing the flower pot opening by fresh-keeping film to increase the humidity of ambient air for one week; removing the fresh keeping film, culturing at the room temperature for one month, and detecting the infection rate. The method has a homologous transplanting success rate of 93% and a heterogenous transplanting success rate of 76%, has the advantages of rapidness, convenience, low cost, high success rate, etc., and overcomes the defect of low success rate in the prior art.

Description

【Technical Field】: [0001] The invention relates to the technical field of strain screening of resistant endophytic fungi and its artificial transfer technology, in particular to the method of artificial transfer to transfer resistant endophytic fungi into turfgrass to obtain resistant turfgrass Kind. 【Background technique】: [0002] Endophytic fungi are a type of fungi that exist in the stems and leaves of healthy plants but do not cause plant diseases. The existence of endophytic fungi has been confirmed in many higher plants, but it is the most common in Gramineae. According to statistics, at least 80 genera and more than 290 species of plants in the Gramineae family are confirmed to contain endophytic fungi (Clay K.1990.Fungal endophytes of grasses.Annual Review of Ecology and Systematics 21:275-297.), but a large number of them Research has focused on two economically important species, namely tall fescue and ryegrass. Existing studies have shown that endophytic fungi and gra...

AI Technical Summary

Problems solved by technology

In a word, as far as the international scope is concerned, the reported successful inoculation techniques have a relatively low success rate on the one hand, and are often protected by patents on the other hand. In my country, there have been no reports on the artificial transfer technology of endophytic fungi, so endophytic fungi The artificial inoculation technology of fungi has become the bottleneck of using endophytic fungi to improve grass resistance breeding, and a breakthrough is urgently needed

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