Application of OsARD1 gene to improving of flooding tolerance of rice

A flood-resistant, genetic technology, applied in applications, genetic engineering, plant genetic improvement, etc., can solve problems such as unknown biological functions

Inactive Publication Date: 2017-05-10
TIANJIN NORMAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently OSARD1 The specific biological function is still unknown, but p

Method used

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  • Application of OsARD1 gene to improving of flooding tolerance of rice
  • Application of OsARD1 gene to improving of flooding tolerance of rice
  • Application of OsARD1 gene to improving of flooding tolerance of rice

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] 1 Materials and methods

[0018] 1.1 Materials

[0019] Japonica rice variety Zhonghua 11 ( Oryza sativa L. ssp. Japonica cv. Zhonghua 11) and its transgenic plants were used in this study. The field materials were all planted in the experimental field of Tianjin Normal University in Tianjin, and the field maintained daily management. Materials used for treatment were grown in climate chambers (Climacell MMM, Germany).

[0020] 1.2 OSARD1 Construction of overexpression vector

[0021]OSARD1 The target gene was obtained by RT-PCR. Total RNA was extracted from 60-day-old mature rice leaves, and cDNA was synthesized by reverse transcription with M-MLV reverse transcriptase (Vazyme, Nanjing). OSARD1 specific primer

[0022] OsARD1-F: 5’-TTAggtaccTTCCACCCCCGCAATCCACAT-3’

[0023] and OsARD1-R: 5'-GTTgtcgacGTGCAGGAGCCCAACAAAAC-3' (lowercase letters are restriction site sequence) to obtain by PCR amplification OSARD1 Complete ORF (Open Reading Frame).

[0024] The...

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Abstract

The invention relates to an application of an OsARD1 gene to improving of the flooding tolerance of rice. The nucleotide sequence of the OsARD1 gene is SEQ ID No.1, and the encoded amino acid sequence of the OsARD1 gene is SEQ ID No.2. The experiment results show that the coleoptile and the lamina of an overexpressed transgenic plant are both longer than those of a wild type control plant, so that the OsARD1 gene overexpressed transgenic plant is more rapidly contacted with air and shine through more rapid elongation of the coleoptile and the lamina under the flooding condition, then the normal aerobic metabolism and the photosynthesis of the plant are recovered, and enough nutrients are guaranteed for the growth of the part flooded by water. Further experiments prove that overexpression of the OsARD1 gene strengthens the flooding tolerance of rice to a certain degree. By improving the flooding tolerance of the rice and cultivating flooding tolerant rice varieties, the loss of the rice in the flood disaster is reduced, and the application is of great significance for guaranteeing food security of people and the agricultural sustainable development.

Description

[0001] This study was supported by the Tianjin Natural Science Foundation Key Project (No. 16JCZDJC33400) and the Tianjin Young and Middle-aged Backbone Teacher Innovation Training Program (ZX110GG017). technical field [0002] The invention belongs to the technical field of improving the overall growth and development of rice, and relates to OSARD1 The application of genes in rice flood tolerance, especially the ability to enhance the flood tolerance of rice and reduce the loss of rice in flood disasters, is of great significance to ensure the food security of the world's people and the sustainable development of agriculture. Background technique [0003] Rice is the largest grain crop in my country and the grain crop with the highest yield per unit area. Its planting area is less than 30% of the grain crops, but its output accounts for more than 40% of the total grain output; more than 60% of the population in my country takes rice as the staple food. During rice productio...

Claims

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Application Information

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IPC IPC(8): C12N15/53C12N9/02A01H5/00
CPCY02A40/138C12N9/0069C12N15/8261C12Y113/11053
Inventor 栾维江熊炜张泗举梁闪闪
Owner TIANJIN NORMAL UNIVERSITY
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