The invention provides a method of physically assisting and facilitating
plant regeneration of
aleurites fordii stem segments with buds. The method includes the following steps of S1, conducting
primary culture, wherein after being disinfected,
aleurites fordii seed embryos are used for inoculating a
primary culture medium untill the seed embryos
bud, and the
primary culture medium comprises 1 / 2 of MS and 0.5-1.0 mg / L of GA3, or 1 / 2 of MS and 0.5 mg / L of IAA; S2, inducing the stem segments with the buds to
bud, wherein the stem segments with the buds on the
aleurites fordii seed embryos in step 1 are
cut off and then used for inoculating an induction medium to be induced to
bud, and the induction medium comprises MS, 3 mg / L of 6-BA and 0.1 mg / L of IBA; S3, conducting rooting treatment, wherein the stem segments with new buds are transferred into a rooting medium, and the rooting medium comprises 1 / 2 of MS, 0.2 mg / L of LIBA, 0.5 g / L of
activated carbon and medical stone which accounts for 1 / 10 of the volume of a container. According to the method of conducting in-vitro rapid propagation on the aleurites fordii stem segments with the buds, by obtaining the stem segments with the budsafter the seed embryos bud aseptically, the bacterium
infection rate is greatly reduced, and the
budding rate is increased; during activation and proliferation of the stem segments with the buds, thesame culture medium is adopted, and by carrying out
light quality control on proliferation indexes, the
workload of preparing different culture media is reduced; by adding additives into the rootingmedium, the rooting rate is greatly increased.