34 results about "Proliferation index" patented technology

The invention discloses a method for studying on prostate cancer reoccurrence and metastasis, not useful in diagnosing and treating diseases. The method is characterized by including: using paraffin-embedded tissue samples containing prostate tissues, making the samples into HE (hematoxylin and eosin) stained tissue slices, making the tissue slices into microarray tissue chips, subjecting the microarray tissue chips to immunohistochemical staining, observing under a microscope, acquiring stained cell scores according to stained cell percentage ranges of the samples, acquiring a positive staining strength coefficient according to staining strength, multiplying the stained cell scores by the positive staining strength coefficient to obtain protein expression level of MS4A8B gene, and determining prostate cancer reoccurrence rate and metastasis rate according to the protein expression level of MS4A8B gene or by subjecting the protein expression level of MSA8B gene and a proliferation index of MS4A8B gene to correlation analysis. Prostate cancer reoccurrence and metastasis are studied herein by detecting the protein expression level and proliferation index of prostate cancer gene in the tissue samples to be detected.

The invention discloses a luminal B type HER-2 negative breast cancer prognostic prediction model KLP-PI and relates to the field of COX algorithm prognostic prediction. The luminal B type HER-2 negative breast cancer prognostic prediction model KLP-PI is obtained based on the Chinese population. KLP-PI=1.0*KI-67LI+1.5*LN+1*PR status, according to a tumor KI-67 proliferation index, KI-67 LI is assigned with a value of 1 if the tumor KI-67 proliferation index is larger than 30%, the KI-67 LI is assigned with a value of 0 if the tumor KI-67 proliferation index is smaller than 30%, LN representslymph node metastasis, the LN is assigned with a value of 1 if the metastasis exists and is assigned with a value of 0 if the metastasis does exist, PR status represents the condition of a progesterone receptor, the PR status is 0 for a positive condition and is 1 for a negative condition, a results KLP-PI value is divided into three groups, scores 0 to 1.5 are in a low-risk group, scores 2 to 2.5are in a medium-risk group, and scores 3 to 5 is in a high-risk group.

The invention discloses a method for analyzing immunoregulation of deer fetus oligopeptides on macrophages, and belongs to the technical field of biotechnology research, development and analysis. After screening, the proliferation index of RAW264.7 cells is significantly higher than that of a blank group, and is closest to that of an LPS positive control group. The method analyzes the influence ofdifferent mass concentrations of deer fetus oligopeptides on the phagocytic function, the NO secretion amount and the release of cytokines such as IL-1 beta, IL-6 and TNF-alpha; and the obtained results show that, under the concentration of 25 mu g / mL, the NO has the maximum release amount and is closest to the LPS group. According to the method, cell cycle inspection is carried out simultaneously; data results prove that with the increase of the mass concentration of the deer fetus oligopeptides, the cell proportion in the G0 / G1 stage is in a trend of firstly rising and then falling, the cell proportion in the S stage and the G2 / M stage is in a trend of firstly falling and then rising, and the proportion of the cells in each stage is closest to that of the LPS group at the concentrationof 25 mu g / mL; the G0 / G1 stage is the synthesis period of RNA and protein, so that the expression quantity of related protein is increased, and the secretion amount of phagocytic action, NO and cytokines reaches the maximum value in the period.

The invention discloses a molecular identification method for the resistance of a rice black streaked dwarf virus (RBSDV). The identification method comprises the following steps of: (1) obtaining brown planthoppers with the virus and an identification ruler; (2) performing quantitative virus transmission to coleoptiles on the brown planthoppers with the virus; (3) performing fluorescent quantitative polymerase chain reaction (PCR) to obtain the relative quantity (RQ) value of each variety cycle time point; and (4) drawing an RBSDV proliferation index trend line and performing resistance evaluation. The molecular identification method has the advantages that the resistance level and resistance grade of rice varieties for the rice black streaked dwarf virus can be accurately identified on the molecular level at the rice sprouting period, the identification period is short, the accuracy is high, and thus the method has relatively high breeding application values.

The invention discloses a molecular identification method for the resistance of a rice black streaked dwarf virus (RBSDV). The identification method comprises the following steps of: (1) obtaining brown planthoppers with the virus and an identification ruler; (2) performing quantitative virus transmission to coleoptiles on the brown planthoppers with the virus; (3) performing fluorescent quantitative polymerase chain reaction (PCR) to obtain the relative quantity (RQ) value of each variety cycle time point; and (4) drawing an RBSDV proliferation index trend line and performing resistance evaluation. The molecular identification method has the advantages that the resistance level and resistance grade of rice varieties for the rice black streaked dwarf virus can be accurately identified on the molecular level at the rice sprouting period, the identification period is short, the accuracy is high, and thus the method has relatively high breeding application values.

The method for improving the immunogenicity of S-adenosyl-L-cysteine ​​hydrolase and the hexasaccharide-modified ahcy protein relate to the field of protein immunogenicity, and the purpose is to provide better vaccine candidate antigens for Brucella. The method is as follows: mix hexasaccharide and sodium periodate at a molar ratio of 1:1 in PBS at pH 6.0 for 1 hour at 4°C; add EDC and NHS at a molar ratio of 1:1 to the above system for 6 hours at room temperature; Add ahcy protein to the above system at a molar ratio of ahcy protein to hexasaccharide of 1:1000 to react overnight; dialyze in 50mmol / L, pH8. Dialyze in PBS of ~8.0 for one day. The stimulation proliferation index, antibody titer, and the amount of IFN-γ and IL-2 produced by stimulating mouse spleen lymphocytes of the obtained hexasaccharide-modified ahcy protein were all greatly improved.

The invention relates to the field of biotechnologies, in particular to a small molecular polypeptide. The polypeptide is provided with an amino acid sequence shown as SEQ ID NO:1. The invention further provides application of the small molecule polypeptide in preparation of drugs for treating colitis and colorectal cancers related to colitis. The colon length, tumor number and the like of a treatment group of rats are obviously lower than those of a control group through treatment of the small molecular polypeptide. The bowel tissue proliferation indexes of rats suffering from the colorectal cancers related to colitis are remarkably reduced. In addition, the small molecular polypeptide can promote enterocyte apoptosis and inhibit enterocyte proliferation in an in-vitro cell experiment. The purpose of blocking inflammatory-cancer conversion is achieved by interfering or blocking biological functions of the polypeptide FKBP11, and novel drug design and a therapeutic target are provided for relieving of colitis related tumors.

The invention relates to the field of immunohistochemical detection, and specifically discloses a method for preparing a standard comparison card for assisting in the interpretation of KI67 proliferation index, comprising the following steps: 1) A fully automatic digital section scanning system scans tumor tissue sections stained by KI67 immunohistochemistry , to obtain the image data; 2) select the hotspot area in the image data, and use the computer to interpret the proportion of KI67 positive cells in the hotspot area to the tumor cells in the hotspot area, which is the KI67 proliferation index; 3) intercept more than one known KI67 proliferation index A picture of the hotspot area. The standard comparison card of the present invention has good accuracy, is not easily affected by the seniority of doctors, and can assist doctors in stably judging the KI67 proliferation index.

The invention discloses a diagnosis and treatment target-PLEK gene of preeclampsia. Whether a subject has the preeclampsia is judged or whether the subject has the risk of being suffered from preeclampsia is diagnosed through detecting the content of a PLEK gene and an expression product thereof in a placental tissue of the subject. Furthermore, a proliferation index of a preeclampsia cell, whichis cultured in vitro, is researched and proves that the PLEK gene is used as a medicine target for treating the preeclampsia.

The invention discloses a method for rapidly evaluating rice SRBSDV variety resistance through detecting a viral multiplication speed in coleoptile. The method comprises the following steps: (1) selecting an identifying scaleplate; (2) performing hypoxia induction on the rice to be tested and identifying the coleoptile of the scaleplate variety; (3) inoculating the toxic sogatella furcifera on the coleoptile and performing dark culture; (4) performing fluorogenic quantitative PCR to obtain a relative transcript level (RQ value) of an SRBSDV virus S9 and a reference gene in each rice variety period time; (5) drawing an SRBSDV proliferation index trend line; and (6) evaluating the resistance level and the resistance grade of the rice variety to be tested on the Southern rice black-streaked dwarf virus according to the SRBSDV proliferation index trend line formula. By detecting the viral multiplication speed in the coleoptiles and rapidly evaluating the resistance of the rice SRBSDV germplasm resistance, the resistance level and the resistance grade of the rice variety on the SRBSDV from the molecular level in the rice germination stage are determined, the identification period is short and the reliability is high, and the method has big breeding application value.

The invention provides a method of physically assisting and facilitating plant regeneration of aleurites fordii stem segments with buds. The method includes the following steps of S1, conducting primary culture, wherein after being disinfected, aleurites fordii seed embryos are used for inoculating a primary culture medium untill the seed embryos bud, and the primary culture medium comprises 1 / 2 of MS and 0.5-1.0 mg / L of GA3, or 1 / 2 of MS and 0.5 mg / L of IAA; S2, inducing the stem segments with the buds to bud, wherein the stem segments with the buds on the aleurites fordii seed embryos in step 1 are cut off and then used for inoculating an induction medium to be induced to bud, and the induction medium comprises MS, 3 mg / L of 6-BA and 0.1 mg / L of IBA; S3, conducting rooting treatment, wherein the stem segments with new buds are transferred into a rooting medium, and the rooting medium comprises 1 / 2 of MS, 0.2 mg / L of LIBA, 0.5 g / L of activated carbon and medical stone which accounts for 1 / 10 of the volume of a container. According to the method of conducting in-vitro rapid propagation on the aleurites fordii stem segments with the buds, by obtaining the stem segments with the budsafter the seed embryos bud aseptically, the bacterium infection rate is greatly reduced, and the budding rate is increased; during activation and proliferation of the stem segments with the buds, thesame culture medium is adopted, and by carrying out light quality control on proliferation indexes, the workload of preparing different culture media is reduced; by adding additives into the rootingmedium, the rooting rate is greatly increased.