34 results about "Proliferation index" patented technology

The present invention discloses a method of applying novel bioinformatics and computational methodologies to data generated by high-resolution genome-wide comparative genomic hybridization and gene expression profiling on CD138-sorted plasma cells from a cohort of 92 newly diagnosed multiple myeloma patients treated with high dose chemotherapy and stem cell rescue. The results revealed that gains the q arm and loss of the p arm of chromosome 1 were highly correlated with altered expression of resident genes in this chromosome, with these changes strongly correlated with 1) risk of death from disease progression, 2) a gene expression based proliferation index, and 3) a recently described gene expression-based high-risk index. Importantly, we also found a strong correlation between copy number gains of 8q24, and increased expression of Argonate 2 (AGO2) a gene coding for a master regulator of microRNA expression and maturation, also being significantly correlated with outcome. Our novel findings significantly improve our understanding of the genomic structure of multiple myeloma and its relationship to clinical outcome.

The invention discloses an in vitro construction method of liver organs and applications. Mouse hepatocytes are extracted to induce to construct liver organs; the forms of the liver organs are observed under a microscope; and immunofluorescence and PCR identification are performed on dryness and epithelial cell properties. An acute hepatic failure mouse model after 70% hepatectomy is established;the liver functions on the first, fourth and seventh day after operation are evaluated after the liver organs are transplanted; liver-to-body ratio is compared; and the treatment effects of the liverorgans on acute hepatic failure are verified through HE staining and immunohistochemistry. Inflammations such as HE staining and ki-67 staining and proliferation indexes show that proliferation can beobviously enhanced after the liver organs are transplanted. The liver organs have strong dryness and proliferation functions; the liver functions of acute hepatic failure mice after 70% hepatectomy can be effectively relieved, and the liver-to-body ratio can be increased; and liver regeneration and repair of acute hepatic failure after 70% hepatectomy can be promoted by relieving inflammatory infiltration of livers.

The invention discloses a method for studying on prostate cancer reoccurrence and metastasis, not useful in diagnosing and treating diseases. The method is characterized by including: using paraffin-embedded tissue samples containing prostate tissues, making the samples into HE (hematoxylin and eosin) stained tissue slices, making the tissue slices into microarray tissue chips, subjecting the microarray tissue chips to immunohistochemical staining, observing under a microscope, acquiring stained cell scores according to stained cell percentage ranges of the samples, acquiring a positive staining strength coefficient according to staining strength, multiplying the stained cell scores by the positive staining strength coefficient to obtain protein expression level of MS4A8B gene, and determining prostate cancer reoccurrence rate and metastasis rate according to the protein expression level of MS4A8B gene or by subjecting the protein expression level of MSA8B gene and a proliferation index of MS4A8B gene to correlation analysis. Prostate cancer reoccurrence and metastasis are studied herein by detecting the protein expression level and proliferation index of prostate cancer gene in the tissue samples to be detected.

The invention discloses a CDSN gene as a diagnosis and treatment target of tongue squamous carcinoma. By detecting content of CDSN gene and its expression product in tissues of a subject, whether the subject is affected with tongue squamous carcinoma can be judged or whether the subject has the risk of suffering from tongue squamous carcinoma can be diagnosed, or a patient's reactivity to drug therapy can be judged, or a patient's relapse and prognosis can be judged. In addition, through the research on proliferation index of tongue squamous carcinoma cells cultured in vitro, it is proved that the CDSN gene and its expression production are used as the drug target for treatment of tongue squamous carcinoma.

The invention relates to the field of biotechnologies, in particular to a small molecular polypeptide. The polypeptide is provided with an amino acid sequence shown as SEQ ID NO:1. The invention further provides application of the small molecule polypeptide in preparation of drugs for treating colitis and colorectal cancers related to colitis. The colon length, tumor number and the like of a treatment group of rats are obviously lower than those of a control group through treatment of the small molecular polypeptide. The bowel tissue proliferation indexes of rats suffering from the colorectal cancers related to colitis are remarkably reduced. In addition, the small molecular polypeptide can promote enterocyte apoptosis and inhibit enterocyte proliferation in an in-vitro cell experiment. The purpose of blocking inflammatory-cancer conversion is achieved by interfering or blocking biological functions of the polypeptide FKBP11, and novel drug design and a therapeutic target are provided for relieving of colitis related tumors.

The invention provides a quantitative analysis system for retinopathy based on image recognition. The system comprises a retina fundus image acquisition module, an image preprocessing module, a nervefiber functional layer upper bound recognition module, a nerve fiber functional layer upper bound recognition module, a nerve fiber functional layer lower bound recognition module, a calculation module and a quantitative analysis result output module. The system has the advantages that the following characteristic parameters for quantitative analysis of retinopathy can be accurately detected and analyzed by adopting the computer intelligent image analysis method: nerve fiber functional layer nuclear number quantity A1, nerve fiber functional layer nuclear total area SA, nerve fiber functionallayer lower bound length L, nerve fiber functional layer area S total, edema index SZ and cell proliferation index PI, thus providing objective accurate detection results for ophthalmic diagnosis or animal retinal pathology research.

The invention provides a method of physically assisting and facilitating plant regeneration of aleurites fordii stem segments with buds. The method includes the following steps of S1, conducting primary culture, wherein after being disinfected, aleurites fordii seed embryos are used for inoculating a primary culture medium untill the seed embryos bud, and the primary culture medium comprises 1 / 2 of MS and 0.5-1.0 mg / L of GA3, or 1 / 2 of MS and 0.5 mg / L of IAA; S2, inducing the stem segments with the buds to bud, wherein the stem segments with the buds on the aleurites fordii seed embryos in step 1 are cut off and then used for inoculating an induction medium to be induced to bud, and the induction medium comprises MS, 3 mg / L of 6-BA and 0.1 mg / L of IBA; S3, conducting rooting treatment, wherein the stem segments with new buds are transferred into a rooting medium, and the rooting medium comprises 1 / 2 of MS, 0.2 mg / L of LIBA, 0.5 g / L of activated carbon and medical stone which accounts for 1 / 10 of the volume of a container. According to the method of conducting in-vitro rapid propagation on the aleurites fordii stem segments with the buds, by obtaining the stem segments with the budsafter the seed embryos bud aseptically, the bacterium infection rate is greatly reduced, and the budding rate is increased; during activation and proliferation of the stem segments with the buds, thesame culture medium is adopted, and by carrying out light quality control on proliferation indexes, the workload of preparing different culture media is reduced; by adding additives into the rootingmedium, the rooting rate is greatly increased.

The invention discloses a method for rapidly evaluating rice SRBSDV variety resistance through detecting a viral multiplication speed in coleoptile. The method comprises the following steps: (1) selecting an identifying scaleplate; (2) performing hypoxia induction on the rice to be tested and identifying the coleoptile of the scaleplate variety; (3) inoculating the toxic sogatella furcifera on the coleoptile and performing dark culture; (4) performing fluorogenic quantitative PCR to obtain a relative transcript level (RQ value) of an SRBSDV virus S9 and a reference gene in each rice variety period time; (5) drawing an SRBSDV proliferation index trend line; and (6) evaluating the resistance level and the resistance grade of the rice variety to be tested on the Southern rice black-streaked dwarf virus according to the SRBSDV proliferation index trend line formula. By detecting the viral multiplication speed in the coleoptiles and rapidly evaluating the resistance of the rice SRBSDV germplasm resistance, the resistance level and the resistance grade of the rice variety on the SRBSDV from the molecular level in the rice germination stage are determined, the identification period is short and the reliability is high, and the method has big breeding application value.

The invention discloses a method for analyzing a regulation effect of deer skin protein peptide on macrophage immune cells, and belongs to the technical field of biotechnology research, development and analysis. By compared with proliferation activities of samples with different concentrations, a result shows that the proliferation index of RAW264.7 cells is increased along with the increase of adeer skin protein peptide concentration, and the concentration is 200[mu]g / mL and is closest to an LPS positive control group; in order to verify the influence of deer skin peptide on immunoregulation, the influence of deer skin protein peptide with different mass concentrations on NO secretion amount, LZM activity and release of cytokines such as IL-6 and TNF-[alpha] is researched, and the resultshows that the NO secretion amount is increased along with the increase of the sample concentration; after the deer skin protein peptide acts for 24 hours, the secretion function of RAW264.7 cytokines is obviously increased, and when the concentration is 50 [mu]g / mL, the levels of all the cytokines are close to those of an LPS group, which indicates that the deer skin protein peptide has the effect of enhancing cytokine secretion; in conclusion, the deer skin protein peptide has a potential effect of enhancing the immunocompetence, and can provide reference for subsequent research.

The invention discloses a culture medium for culturing olfactory ensheathing cells. The culture medium provided by the invention comprises components 1-10. Proved by experiments, the culture medium provided by the invention is used for culturing olfactory ensheathing cells of primates, and basically has no feeder cells; the olfactory ensheathing cells obtained through culturing are high in proliferation index; and meanwhile, the proficiency of stem cells differentiating into entoderm, mesoderm and ectoderm tissue derivatives is maintained, and the karyotype of the stem cells is maintained.

The invention provides a method for improving S-adenosyl-L-cysteine hydrolase immunogenicity and hexasaccharide modified ahcy protein, relates to the field of protein immunogenicity, aiming at providing better vaccine candidate antigens for Brucella. The method comprises the steps of mixing hexasaccharide and sodium periodate at 4 DEG C in a PBS of which the pH is 6.0 according to the molar ratio of 1 to 1, and reacting for 1h; adding EDC and NHS at normal temperature into a system according to the molar ratio of 1 to 1, and reacting for 6h; adding ahcy protein into the system at the normal temperature according to the molar ratio of 1 to 10000 of the ahcy protein to the hexasaccharide, and reacting overnight; dialyzing at 4 DEG C for 5h in a carbonate solution of which the volume is 50mmol / L and the pH is 8.0 to 9.6, and then dialyzing for one day in the PBS of which the volume is 50mmol / L and the pH is 7.0 to 8.0. A stimulation proliferation index, an antibody titer, the amount of IFN-gamma and IL-2 generated by stimulating mice spleen lymphocyte of the obtained hexasaccharide modified ahcy protein are greatly improved.

The invention discloses a CLIC3 gene which is a lung adenocarcinoma diagnosis and treatment target. By detecting the content of the CLIC3 gene and expression products thereof in the lung tissue of a testee, whether the testee has lung adenocarcinoma or whether the testee is likely to develop lung adenocarcinoma can be judged, and the reactivity of a patient to drug therapy and relapse and prognosis conditions of the patient can be judged. Furthermore, by studying the proliferation index of lung adenocarcinoma cells cultured in vitro, it is proved that the CLIC3 gene and expression products thereof can serve as a drug target for treating lung adenocarcinoma.

The invention relates to a tumor prognosis predicting marker and a detection method thereof. The marker is a lymphoid node tissue; the detection method of the marker comprises the following steps of dewaxing, hydrating, heating at high pressure, interdicting endogenous peroxidase and the like. According to the tumor prognosis predicting marker and the detection method thereof provided by the invention, the marker is detected through the steps of dewaxing, hydrating, heating at high pressure, interdicting endogenous peroxidase and the like; multiple expression groups with different degrees areobtained according to different Ki67 proliferation indexes. The invention provides a new marker and a specific detection method thereof for tumor prognosis predicting, so that the occurrence of the phenomena of inconformity in detection and even opposite results on an immunohistochemical basis is avoided.

The invention discloses a luminal B type HER-2 negative breast cancer prognostic prediction model KLP-PI and relates to the field of COX algorithm prognostic prediction. The luminal B type HER-2 negative breast cancer prognostic prediction model KLP-PI is obtained based on the Chinese population. KLP-PI=1.0*KI-67LI+1.5*LN+1*PR status, according to a tumor KI-67 proliferation index, KI-67 LI is assigned with a value of 1 if the tumor KI-67 proliferation index is larger than 30%, the KI-67 LI is assigned with a value of 0 if the tumor KI-67 proliferation index is smaller than 30%, LN representslymph node metastasis, the LN is assigned with a value of 1 if the metastasis exists and is assigned with a value of 0 if the metastasis does exist, PR status represents the condition of a progesterone receptor, the PR status is 0 for a positive condition and is 1 for a negative condition, a results KLP-PI value is divided into three groups, scores 0 to 1.5 are in a low-risk group, scores 2 to 2.5are in a medium-risk group, and scores 3 to 5 is in a high-risk group.

The invention discloses application of a molecular marker in preparing a drug for diagnosing and treating preeclampsia, and relates to the application of an MBOAT1 gene in preeclampsia. Content of theMBOAT1 gene and an expression product thereof in a placenta tissue of a subject can be detected to judge whether the subject has the risk of suffering from the preeclampsia or not or to diagnose whether the subject has the risk of suffering from the preeclampsia or not. Besides, proliferation indexes of in-vitro cultured placental trophoblast cells are researched to prove that the MBOAT1 gene canbe used as a drug target for treating preeclampsia.

The invention discloses a diagnosis and treatment target-PLEK gene of preeclampsia. Whether a subject has the preeclampsia is judged or whether the subject has the risk of being suffered from preeclampsia is diagnosed through detecting the content of a PLEK gene and an expression product thereof in a placental tissue of the subject. Furthermore, a proliferation index of a preeclampsia cell, whichis cultured in vitro, is researched and proves that the PLEK gene is used as a medicine target for treating the preeclampsia.

The invention discloses a method for analyzing immunoregulation of deer fetus oligopeptides on macrophages, and belongs to the technical field of biotechnology research, development and analysis. After screening, the proliferation index of RAW264.7 cells is significantly higher than that of a blank group, and is closest to that of an LPS positive control group. The method analyzes the influence ofdifferent mass concentrations of deer fetus oligopeptides on the phagocytic function, the NO secretion amount and the release of cytokines such as IL-1 beta, IL-6 and TNF-alpha; and the obtained results show that, under the concentration of 25 mu g / mL, the NO has the maximum release amount and is closest to the LPS group. According to the method, cell cycle inspection is carried out simultaneously; data results prove that with the increase of the mass concentration of the deer fetus oligopeptides, the cell proportion in the G0 / G1 stage is in a trend of firstly rising and then falling, the cell proportion in the S stage and the G2 / M stage is in a trend of firstly falling and then rising, and the proportion of the cells in each stage is closest to that of the LPS group at the concentrationof 25 mu g / mL; the G0 / G1 stage is the synthesis period of RNA and protein, so that the expression quantity of related protein is increased, and the secretion amount of phagocytic action, NO and cytokines reaches the maximum value in the period.

The invention discloses a maize rough dwarf disease resistance identification method, which includes the following steps of: 1) selecting an identification ruler; 2) low-oxygen-inducing coleoptiles of to-be-tested maize and the identification ruler; 3) inoculating toxic laodelphax striatellus to the coleoptiles and dark-culturing the coleoptiles; 4) performing fluorogenic quantitative PCR to obtain relative expression quantity (RQ value) of RBSDV virus S7 and reference genes of each maize variety at periodical time points; 5) drawing a RBSDV proliferation index tendency line; 6) according to a RBSDV proliferation index tendency line equation, evaluating the resistance level and resistance grade of the to-be-tested maize varieties against the maize rough dwarf disease. The resistance level of the maize varieties against the maize rough dwarf disease is evaluated by means of the difference of proliferation tendency of the RBSDV in the coleoptiles, so that the method, on molecular level, can determine the resistance level and resistance grade of the maize varieties against the maize rough dwarf disease in a short time. The method is short in identification period and is high in reliability, and has great application value in seed breeding.

The invention relates to the field of immunohistochemical detection, and particularly discloses a preparation method of a standard comparison card for assisting in interpretation of a KI67 proliferation index. The method comprises the following steps: 1) scanning tumor tissue sections chemically stained by KI67 immunohistochemistry by a full-automatic digital section scanning system to obtain image data; 2) selecting a hot spot area in the image data, and interpreting the proportion of the KI67 positive cells in the hot spot area in tumor cells in the hot spot area by using a computer to obtain a KI67 proliferation index; 3) capturing more than one picture of the hot spot region with the known KI67 proliferation index. The standard comparison card provided by the invention is advantageousin that the accuracy is good, the influence by the qualification age limit of doctors is not easy, and the doctors can be assisted to stably judge the KI67 proliferation indexes.

The invention discloses a method for rapid evaluation of variety resistance to bushy stunt of wheat by detecting proliferation rate of virus in coleoptiles. The method comprises the following steps: 1) selecting identification scale; 2) performing hypoxia induction on wheat to be detected and identifying the coleoptiles of scale variety; 3) inoculating viruliferous laodelphax striatellus into the coleoptiles and then performing dark culture; 4) performing fluorescence quantitative PCR (polymerase chain reaction) to obtain relative expression (RQ value) of RBSDV (rice black streaked dwarf virus) virus S7 and an internal control gene of various wheat varieties at period time points; 5) drawing an RBSDV proliferation index trend line; and 6) evaluating the resistance level and the resistance grade of the wheat variety to be detected to the bushy stunt of the wheat according to the RBSDV proliferation index trend line formula. According to the method disclosed by the invention, the variety resistance to the bushy stunt of the wheat is rapidly evaluated by detecting the proliferation rate of the virus in the coleoptiles, the resistance level and the resistance grade of the wheat variety to the bushy stunt of the wheat can be rapidly determined on molecular scale, the identification period is short and the reliability is high, thereby having relatively great breeding application value.

The invention discloses a molecular identification method for the resistance of a rice black streaked dwarf virus (RBSDV). The identification method comprises the following steps of: (1) obtaining brown planthoppers with the virus and an identification ruler; (2) performing quantitative virus transmission to coleoptiles on the brown planthoppers with the virus; (3) performing fluorescent quantitative polymerase chain reaction (PCR) to obtain the relative quantity (RQ) value of each variety cycle time point; and (4) drawing an RBSDV proliferation index trend line and performing resistance evaluation. The molecular identification method has the advantages that the resistance level and resistance grade of rice varieties for the rice black streaked dwarf virus can be accurately identified on the molecular level at the rice sprouting period, the identification period is short, the accuracy is high, and thus the method has relatively high breeding application values.

The invention relates to a system for predicting prognosis based on pathological morphology characteristics and proliferation indexes of pancreatic solid pseudopapilloma. The invention relates to an operation excision specimen for pancreatic solid pseudopapilloma, which comprises the following steps of: firstly, preparing a hematoxylin-eosin staining (HE) slice by fully taking materials, judging whether lymphatic vessel invasion (LVI) exists or not by using a light microscope, and then evaluating a tumor proliferation index (Ki-67,%) by using an immunohistochemical staining (IHC) method. According to an evaluation system, if LVI exists and Ki-67 is greater than or equal to 3%, a disease progression high-risk group is determined; lVI and Ki-67 lt exist in the sample; if LVI does not exist and Ki-67 is greater than or equal to 3%, the group is a medium-risk group; lVI and Ki-67 lt do not exist; and 3% of the group is a low-risk group. The system can accurately predict the progression condition of pancreatic solid pseudopapilloma, and is more effective than the eighth version of pancreatic cancer staging of the American United Cancer (AJCC) which is internationally applied at present. In addition, the invention also proposes that the pancreatic solid pseudopapilloma is excellent in prognosis and should be determined as a benign or junctional tumor according to the risk degree of the pancreatic solid pseudopapilloma.

The invention provides a rapid propagation method for cultivating dennstaeditiaceae suspension cells. The method comprises the following steps: obtaining of sterile blades; induction of calluses; proliferation of the calluses; cultivation of suspension cells and the like. The dennstaeditiaceae suspension cells prepared by the method disclosed by the invention are high in proliferation index, short in growth period, and good in genetic stability, and a new path is provided for development and utilization of dennstaeditiaceae medicinal secondary metabolites.

The invention discloses a molecular identification method for the resistance of a rice black streaked dwarf virus (RBSDV). The identification method comprises the following steps of: (1) obtaining brown planthoppers with the virus and an identification ruler; (2) performing quantitative virus transmission to coleoptiles on the brown planthoppers with the virus; (3) performing fluorescent quantitative polymerase chain reaction (PCR) to obtain the relative quantity (RQ) value of each variety cycle time point; and (4) drawing an RBSDV proliferation index trend line and performing resistance evaluation. The molecular identification method has the advantages that the resistance level and resistance grade of rice varieties for the rice black streaked dwarf virus can be accurately identified on the molecular level at the rice sprouting period, the identification period is short, the accuracy is high, and thus the method has relatively high breeding application values.

The method for improving the immunogenicity of S-adenosyl-L-cysteine ​​hydrolase and the hexasaccharide-modified ahcy protein relate to the field of protein immunogenicity, and the purpose is to provide better vaccine candidate antigens for Brucella. The method is as follows: mix hexasaccharide and sodium periodate at a molar ratio of 1:1 in PBS at pH 6.0 for 1 hour at 4°C; add EDC and NHS at a molar ratio of 1:1 to the above system for 6 hours at room temperature; Add ahcy protein to the above system at a molar ratio of ahcy protein to hexasaccharide of 1:1000 to react overnight; dialyze in 50mmol / L, pH8. Dialyze in PBS of ~8.0 for one day. The stimulation proliferation index, antibody titer, and the amount of IFN-γ and IL-2 produced by stimulating mouse spleen lymphocytes of the obtained hexasaccharide-modified ahcy protein were all greatly improved.

The invention relates to the field of biotechnologies, in particular to a small molecular polypeptide. The polypeptide is provided with an amino acid sequence shown as SEQ ID NO:1. The invention further provides application of the small molecule polypeptide in preparation of drugs for treating colitis and colorectal cancers related to colitis. The colon length, tumor number and the like of a treatment group of rats are obviously lower than those of a control group through treatment of the small molecular polypeptide. The bowel tissue proliferation indexes of rats suffering from the colorectal cancers related to colitis are remarkably reduced. In addition, the small molecular polypeptide can promote enterocyte apoptosis and inhibit enterocyte proliferation in an in-vitro cell experiment. The purpose of blocking inflammatory-cancer conversion is achieved by interfering or blocking biological functions of the polypeptide FKBP11, and novel drug design and a therapeutic target are provided for relieving of colitis related tumors.

The invention relates to the field of immunohistochemical detection, and specifically discloses a method for preparing a standard comparison card for assisting in the interpretation of KI67 proliferation index, comprising the following steps: 1) A fully automatic digital section scanning system scans tumor tissue sections stained by KI67 immunohistochemistry , to obtain the image data; 2) select the hotspot area in the image data, and use the computer to interpret the proportion of KI67 positive cells in the hotspot area to the tumor cells in the hotspot area, which is the KI67 proliferation index; 3) intercept more than one known KI67 proliferation index A picture of the hotspot area. The standard comparison card of the present invention has good accuracy, is not easily affected by the seniority of doctors, and can assist doctors in stably judging the KI67 proliferation index.

The invention discloses a method for rapidly evaluating rice SRBSDV variety resistance through detecting a viral multiplication speed in coleoptile. The method comprises the following steps: (1) selecting an identifying scaleplate; (2) performing hypoxia induction on the rice to be tested and identifying the coleoptile of the scaleplate variety; (3) inoculating the toxic sogatella furcifera on the coleoptile and performing dark culture; (4) performing fluorogenic quantitative PCR to obtain a relative transcript level (RQ value) of an SRBSDV virus S9 and a reference gene in each rice variety period time; (5) drawing an SRBSDV proliferation index trend line; and (6) evaluating the resistance level and the resistance grade of the rice variety to be tested on the Southern rice black-streaked dwarf virus according to the SRBSDV proliferation index trend line formula. By detecting the viral multiplication speed in the coleoptiles and rapidly evaluating the resistance of the rice SRBSDV germplasm resistance, the resistance level and the resistance grade of the rice variety on the SRBSDV from the molecular level in the rice germination stage are determined, the identification period is short and the reliability is high, and the method has big breeding application value.

The invention provides a method of physically assisting and facilitating plant regeneration of aleurites fordii stem segments with buds. The method includes the following steps of S1, conducting primary culture, wherein after being disinfected, aleurites fordii seed embryos are used for inoculating a primary culture medium untill the seed embryos bud, and the primary culture medium comprises 1 / 2 of MS and 0.5-1.0 mg / L of GA3, or 1 / 2 of MS and 0.5 mg / L of IAA; S2, inducing the stem segments with the buds to bud, wherein the stem segments with the buds on the aleurites fordii seed embryos in step 1 are cut off and then used for inoculating an induction medium to be induced to bud, and the induction medium comprises MS, 3 mg / L of 6-BA and 0.1 mg / L of IBA; S3, conducting rooting treatment, wherein the stem segments with new buds are transferred into a rooting medium, and the rooting medium comprises 1 / 2 of MS, 0.2 mg / L of LIBA, 0.5 g / L of activated carbon and medical stone which accounts for 1 / 10 of the volume of a container. According to the method of conducting in-vitro rapid propagation on the aleurites fordii stem segments with the buds, by obtaining the stem segments with the budsafter the seed embryos bud aseptically, the bacterium infection rate is greatly reduced, and the budding rate is increased; during activation and proliferation of the stem segments with the buds, thesame culture medium is adopted, and by carrying out light quality control on proliferation indexes, the workload of preparing different culture media is reduced; by adding additives into the rootingmedium, the rooting rate is greatly increased.