Maize rough dwarf disease resistance identification method

A technology for maize rough dwarf disease and an identification method, which is applied in the field of maize rough dwarf resistance identification, and can solve the problems of inaccurate identification results and long identification period.

Inactive Publication Date: 2017-03-15
WUXI NANLIGONG TECH DEV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0011] The purpose of the present invention is to provide a short identification period and high reliability identification method for corn resistance to rough dwarf disease in view of the shortcomings of the current identification method of corn resistance to rough dwarf disease, which has long identification period and inaccurate identification results.

Method used

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  • Maize rough dwarf disease resistance identification method
  • Maize rough dwarf disease resistance identification method
  • Maize rough dwarf disease resistance identification method

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Embodiment

[0049] In 2014, the identification method of the invention was used to identify the resistance of some of the collected corn variety resources to rough dwarf disease, and three corn varieties, Dan 340, Zhutian 1, and Jidan 7, were set as high-resistant and medium-resistant corn varieties. The identification scale of resistance and susceptibility, among them, Dan 340 ranked 7th among 68 corn variety resource groups in the indoor infection identification in 2012-2013, Zhutian 1 ranked 17th, and Jidan 7 ranked 34th; The concentration of the treated IAA solution is selected to be 2.5 μmol / L; the concentration of the ABA solution is selected to be 40 μmol / L; the seeds are placed at a place 8 cm below the liquid surface to induce coleoptiles; the quantitative inoculation of poisonous SBPH is controlled at 12 worms / coleoptile; The coleoptile collection time points were set as four time points on the 5th, 8th, 11th, and 14th days after the completion of inoculation.

[0050] Real-time...

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Abstract

The invention discloses a maize rough dwarf disease resistance identification method, which includes the following steps of: 1) selecting an identification ruler; 2) low-oxygen-inducing coleoptiles of to-be-tested maize and the identification ruler; 3) inoculating toxic laodelphax striatellus to the coleoptiles and dark-culturing the coleoptiles; 4) performing fluorogenic quantitative PCR to obtain relative expression quantity (RQ value) of RBSDV virus S7 and reference genes of each maize variety at periodical time points; 5) drawing a RBSDV proliferation index tendency line; 6) according to a RBSDV proliferation index tendency line equation, evaluating the resistance level and resistance grade of the to-be-tested maize varieties against the maize rough dwarf disease. The resistance level of the maize varieties against the maize rough dwarf disease is evaluated by means of the difference of proliferation tendency of the RBSDV in the coleoptiles, so that the method, on molecular level, can determine the resistance level and resistance grade of the maize varieties against the maize rough dwarf disease in a short time. The method is short in identification period and is high in reliability, and has great application value in seed breeding.

Description

technical field [0001] The invention relates to the field of agricultural high-tech, in particular to an identification method for maize resistance to rough dwarf disease with short identification period and high reliability. Background technique [0002] Maize belongs to the Poaceae Zea family Zea genus. Its domestication origin can be traced back to 7000-10000 years ago, and it is currently the largest crop in the world. my country is the second largest corn producer in the world after the United States. In recent years, corn has also become the largest crop in my country. It is an important ration, feed grain, industrial raw material and energy raw material, and plays a pivotal role in production. In recent years, with the adjustment of the industrial structure and the continuous increase of the population, the demand for the yield and quality of corn has become higher and higher, and the planting area of ​​corn has also increased year by year. [0003] Maize rough dwarf...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12Q1/70
CPCC12Q1/6895C12Q2600/13
Inventor 不公告发明人
Owner WUXI NANLIGONG TECH DEV
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