Maize rough dwarf disease resistance identification method
A technology for maize rough dwarf disease and an identification method, which is applied in the field of maize rough dwarf resistance identification, and can solve the problems of inaccurate identification results and long identification period.
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[0049] In 2014, the identification method of the invention was used to identify the resistance of some of the collected corn variety resources to rough dwarf disease, and three corn varieties, Dan 340, Zhutian 1, and Jidan 7, were set as high-resistant and medium-resistant corn varieties. The identification scale of resistance and susceptibility, among them, Dan 340 ranked 7th among 68 corn variety resource groups in the indoor infection identification in 2012-2013, Zhutian 1 ranked 17th, and Jidan 7 ranked 34th; The concentration of the treated IAA solution is selected to be 2.5 μmol / L; the concentration of the ABA solution is selected to be 40 μmol / L; the seeds are placed at a place 8 cm below the liquid surface to induce coleoptiles; the quantitative inoculation of poisonous SBPH is controlled at 12 worms / coleoptile; The coleoptile collection time points were set as four time points on the 5th, 8th, 11th, and 14th days after the completion of inoculation.
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