Method of physically assisting and facilitating plant regeneration of aleurites fordii stem segments with buds

Abstract

The invention provides a method of physically assisting and facilitating plant regeneration of aleurites fordii stem segments with buds. The method includes the following steps of S1, conducting primary culture, wherein after being disinfected, aleurites fordii seed embryos are used for inoculating a primary culture medium untill the seed embryos bud, and the primary culture medium comprises 1/2 of MS and 0.5-1.0 mg/L of GA3, or 1/2 of MS and 0.5 mg/L of IAA; S2, inducing the stem segments with the buds to bud, wherein the stem segments with the buds on the aleurites fordii seed embryos in step 1 are cut off and then used for inoculating an induction medium to be induced to bud, and the induction medium comprises MS, 3 mg/L of 6-BA and 0.1 mg/L of IBA; S3, conducting rooting treatment, wherein the stem segments with new buds are transferred into a rooting medium, and the rooting medium comprises 1/2 of MS, 0.2 mg/L of LIBA, 0.5 g/L of activated carbon and medical stone which accounts for 1/10 of the volume of a container. According to the method of conducting in-vitro rapid propagation on the aleurites fordii stem segments with the buds, by obtaining the stem segments with the budsafter the seed embryos bud aseptically, the bacterium infection rate is greatly reduced, and the budding rate is increased; during activation and proliferation of the stem segments with the buds, thesame culture medium is adopted, and by carrying out light quality control on proliferation indexes, the workload of preparing different culture media is reduced; by adding additives into the rootingmedium, the rooting rate is greatly increased.

Description

technical field [0001] The invention belongs to the technical field of tung tree tissue culture, and in particular relates to a method for physically assisting the regeneration of tung tree bud stem segments. Background technique [0002] Tung tree (Vernicia fordii), also known as three-year tree, is a deciduous tree belonging to Euphorbiaceae, and it is a characteristic economic forest tree species in my country. There are 3 species of tung trees: tung oil, tung millennium and Japanese tung oil, among which the tung oil extracted from tung fruit is of the best quality. Tung oil is the basic raw material for the manufacture of high-quality paints and inks, and tung oil can also be developed and utilized as excellent biomass diesel and other industrial raw materials. Aloe tung tree may become one of the most promising biomass energy tree species to alleviate the energy shortage problem in our country. The world's appeal for green environmental protection has led to a surge ...

AI Technical Summary

Problems solved by technology

At present, researches on tissue culture of tung tree focus more on the in vitro culture of seed embryos, petioles and hypocotyls, and most of them are stage-based optimization of different culture media. The use of different culture media at different stages increases the workload, which is not conducive to the later stage. Factory growth, the growth process is cumbersome and the cost is high

At the same time, there is a lack of relevant research on physical factors such as different light qualities on the growth of tissue culture seedlings.

Therefore need to develop the method for the regeneration plant of new tung tree band bud stem section now, to solve the technical problems such as the higher rate of bacteria contamination in the tissue culture method of tung tree in the prior art, the many types of culture medium at different stages

Images