2results about How to "Strong regenerative" patented technology

This invention discloses a network-like porous poly(3,4-hydroxybutyrate) microsphere, its preparation method, and its applications, belonging to the field of biomedical materials technology. The microspheres exhibit a villous network structure with a continuous, irregular "ridge-valley" topology on their surface. The network-like porous poly(3,4-hydroxybutyrate) microspheres provided by this invention maintain good structural integrity even after 6 months of in vivo implantation, significantly increase ATP levels in fibroblasts under glucose-free conditions, and inhibit cell senescence. They also induce macrophages to transform from a pro-inflammatory M1 phenotype to a pro-repair M2 phenotype, avoiding the excessive inflammatory response commonly seen in PLLA-type microspheres, and demonstrate superior collagen regeneration promotion compared to PLLA-type microspheres. Furthermore, they facilitate cell adhesion and proliferation.

This invention relates to the field of animal model construction technology, and more particularly to a method for constructing a zebrafish coronary artery microcirculation injury model based on enzyme stripping and its application. This invention, for the first time, utilizes a rationally composed mixture of collagenases to specifically strip coronary artery endothelial cells, minimizing non-specific damage to cardiomyocytes and stably inducing coronary artery injury to construct a zebrafish model of coronary artery injury, particularly coronary artery microcirculation injury. This model not only has a highly localized injury area and minimal impact on the myocardium, but also exhibits a low mortality rate. Furthermore, the zebrafish model of coronary artery injury constructed by this invention demonstrates significant regenerative potential, with its blood vessels exhibiting strong regenerative capacity. It also shows high sensitivity and responsiveness to drugs, making it potentially valuable in drug screening and mechanism research.