Screening method for pear homologous transgene functional verification material

Abstract

The invention discloses a screening method for a pear homologous transgene functional verification material. The method comprises the following steps that 1, a pear regeneration optimum individual is screened, wherein single-plant fruit picking and seed extraction, single-plant seed sowing and multi-time regeneration culture modes are performed on multiple plants of pears with the good field character expression to screen out the single plant with the best adventive bud regeneration effect to be adopted as the pear regeneration optimum individual; 2, a pear transgenic plant is obtained, wherein an efficient regeneration, rooting and transgenic system is further built on the basis of the screened pear regeneration optimum individual, and the transgenic plant is obtained through antibiotic screening. The method is easy to implement, materials are convenient to obtain, the cost is low, and the method is applicable to all pear materials; an existing general regeneration medium can be utilized for further screening the optimum transgenic material and building the efficient regeneration, rooting and transgenic system, and technical supports are provided for pear genetic transformation.

Description

technical field [0001] The invention relates to the field of high-efficiency genetic transformation systems, in particular to a screening method for pear homologous transgene function verification materials. Background technique [0002] Pear is a fruit tree that is mainly propagated by grafting. A reasonable pear rootstock can improve the abiotic stress tolerance, fruit quality, high yield, maturity period, etc. of the plant to a certain extent. How to obtain a pear rootstock with better traits has always been the effort of breeders. The goal. In pear rootstock breeding, pear rootstock excellent lines with excellent traits are generally obtained through traditional cross breeding. However, the traditional hybridization method takes a long time, the target traits should not be locked, and the workload is heavy. Therefore, under the background of the rapid development of biotechnology, obtaining new transgenic pear rootstock materials with obvious target traits through gene...

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Problems solved by technology

At the same time, the homologous transformation of genes is also a necessary means for functional verification of genes controlling special traits in pears. Usually, the establishment of an efficient regeneration system for in vitro culture is the basis of genetic engineering for fruit trees. However, the regeneration rate of Asian pears is low and there are strong The genotype dependence of these plants limits the functional verification of homologous genes through transgenic methods to a certain extent, which affects the reliability of the functional analysis of related genes in such plants, and also affects the feasibility of obtaining new materials through genetic engineering.

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