Boric acid affinity rutin molecularly imprinted magnetic nanosphere as well as preparation method and application thereof

A technology of magnetic nanospheres and molecular imprinting, applied in separation methods, chemical instruments and methods, and other chemical processes, can solve the problems of low purity of rutin, and achieve simple and easy preparation methods, good water dispersibility, and enrichment Gentle effect of extraction method

Pending Publication Date: 2021-05-11
十堰市太和医院(湖北医药学院附属医院) +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In order to overcome the shortcomings of the above-mentioned prior art, the object of the present invention is to provide a boric acid-affinity rutin molecularly imprinted magnetic nanosphere and its preparation method and application, so as to solve the problem that the purity of rutin extracted by the existing rutin separation and purification method is relatively low. low problem

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  • Boric acid affinity rutin molecularly imprinted magnetic nanosphere as well as preparation method and application thereof
  • Boric acid affinity rutin molecularly imprinted magnetic nanosphere as well as preparation method and application thereof
  • Boric acid affinity rutin molecularly imprinted magnetic nanosphere as well as preparation method and application thereof

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Embodiment 1

[0033] A method for preparing boric acid-affinity rutin molecularly imprinted magnetic nanospheres, comprising the following steps:

[0034] Step 1. Put 0.3g of ferric chloride, 1.0g of sodium acetate, 0.05g of sodium polyacrylate, 6mL of ethylene glycol and 10mL of diethylene glycol in a reaction kettle, and react at 150°C for 5 hours. After the reaction, put The reaction product was washed with ultrapure water until neutral. Then, the obtained product was dried at 30° C. and 0.05 MPa for 3 hours to obtain carboxyl magnetic nanospheres.

[0035]Step 2: 150 mg of carboxyl magnetic nanospheres, 150 mg of EDC and 100 mg of NHS were dissolved and dispersed in ultrapure water, and stirred and reacted at room temperature for 30 min. Then add 150 mg of 3-aminophenylboronic acid and stir for 4 h. After the reaction is completed, the reaction product is washed with ultrapure water until neutral. Then, the obtained product was dried at 30°C and 0.05MPa for 3 hours to obtain phenylbor...

Embodiment 2

[0047] A method for preparing boric acid-affinity rutin molecularly imprinted magnetic nanospheres, comprising the following steps:

[0048] Step 1. Put 0.5g of ferric chloride, 2.0g of sodium acetate, 0.08g of sodium polyacrylate, 7mL of ethylene glycol and 12mL of diethylene glycol in a reaction kettle, and react at 160°C for 8 hours. After the reaction, put The reaction product was washed with ultrapure water until neutral. Then, the obtained product was dried at 55° C. and 0.07 MPa for 6 hours to obtain carboxyl magnetic nanospheres;

[0049] Step 2: 180 mg of carboxyl magnetic nanospheres, 180 mg of EDC and 150 mg of NHS were dissolved and dispersed in ultrapure water, and stirred and reacted at room temperature for 40 min. Then add 180 mg of 3-aminophenylboronic acid and stir for 5 h. After the reaction, the reaction product is washed with ultrapure water until neutral. Then, the obtained product was dried at 55° C. and 0.07 MPa for 6 hours to obtain phenylboronic acid...

Embodiment 3

[0061] A method for preparing boric acid-affinity rutin molecularly imprinted magnetic nanospheres, comprising the following steps:

[0062] Step 1. Put 0.8g of ferric chloride, 3.0g of sodium acetate, 0.08g of sodium polyacrylate, 8.0mL of ethylene glycol and 14mL of diethylene glycol in a reactor, and react at 170°C for 10 hours. After the reaction, The reaction product was washed with ultrapure water until neutral. Then, vacuum-dry the obtained product at 40°C and 0.08MPa for 6.5h to obtain carboxyl magnetic nanospheres;

[0063] Step 2: 200 mg of carboxyl magnetic nanospheres, 200 mg of EDC and 160 mg of NHS were dissolved and dispersed in ultrapure water, and stirred and reacted at room temperature for 60 min. Add 2400 mg of 3-aminophenylboronic acid and stir for 5 h. After the reaction, the reaction product is washed with ultrapure water until neutral. Then, the obtained product was dried at 50°C and 0.07MPa for 6 hours to obtain phenylboronic acid-modified magnetic na...

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Abstract

The invention discloses boric acid affinity rutin molecularly imprinted magnetic nanospheres as well as a preparation method and an application thereof, and belongs to the technical field of nano material preparation. The method comprises the following steps of: modifying a phenylboronic acid group on the surface of a carboxyl magnetic sphere through amido bond connection, taking the obtained phenylboronic acid modified magnetic nanosphere as a carrier, taking rutin as a template molecule, taking polyethyleneimine as a hydrophilic functional monomer, and adopting a method of combining two-step template fixation and surface imprinting. The rutin molecularly imprinted magnetic nanospheres prepared by the method disclosed by the invention are rich in functional monomer groups, uniform in particle size, good in water dispersibility and good in selectivity; the method is a novel method for preparing the rutin molecularly imprinted polymer with high affinity and high selectivity, and shows a wide application prospect in the aspects of selective extraction and separation of the active pharmaceutical ingredient rutin in plants and the like.

Description

technical field [0001] The invention belongs to the technical field of nanomaterial preparation, and in particular relates to a boric acid-affinity rutin molecularly imprinted magnetic nanosphere and a preparation method and application thereof. Background technique [0002] Rutin is a widely distributed natural flavonoid compound. As a natural medicinal active ingredient, rutin has great medical potential in the treatment of cardiovascular diseases, cerebrovascular diseases, cancer, inflammation, etc. due to its remarkable antioxidant, antiviral, anti-inflammatory and anti-free radical properties. The traditional enrichment methods of rutin mainly include solvent extraction, supercritical fluid extraction, microwave-assisted extraction and macroporous adsorption resin. However, most of these methods have problems such as time-consuming, cumbersome operation and poor selectivity, which lead to difficulties in the extraction of rutin and low purity. Therefore, improving the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/26B01J20/28B01J20/30B01D15/00
CPCB01J20/268B01J20/28009B01D15/00
Inventor 汤祥军宋慧佳高瑞霞罗杰付锐张力雷攀
Owner 十堰市太和医院(湖北医药学院附属医院)
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