High-cadmium-adsorption filamentous fungi penicillium chrysogenum J-5 as well as preparation method and application thereof

Abstract

The invention discloses high-cadmium-adsorption filamentous fungi penicillium chrysogenum J-5 as well as a preparation method and an application thereof. The preparation method comprises the following steps of: firstly weighing and putting 10g of cadmium polluted soil into 90mL of sterilized normal saline, vibrating on a constant-temperature shaking table, standing, taking supernatant liquid and diluting; secondly coating different dilution factors of diluent on a Martin rose-bengal flat plate containing 10mM of cadmium, and inversely placing the flat plate in a constant-temperature incubator for cultivation; thirdly, carrying out streaking and purification on microorganisms which grow on the flat plate, and then coating the organisms on the Martin rose-bengal flat plate containing cadmium again for cultivation; fourthly, after isolation and screening, storing a bacterial strain having stable resistance capacity on cadmium on a PDA (Potato Dextrose Agar) inclined plane. The obtained filamentous fungi penicillium chrysogenum J-5 has high tolerance and high adsorption property on multiple metals; the raw materials are easy to obtain and low in cost. The bacterial strain has relatively high fixation capacity on heavy metals in environments, can be used of reducing the biological toxic property of the heavy metals and is suitable for the in-situ remediation of large-area polluted soil.

Description

technical field

[0001] The invention belongs to the field of environmental governance. More specifically, it relates to a preparation method of a filamentous fungus with high tolerance and high adsorption capacity to various heavy metals, and also relates to the use of a filamentous fungus Penicillium chrysogenum J-5. Background technique

[0002] Cadmium (Cd) mainly exists in nature in the form of 13 minerals such as sulfur cadmium ore, and is often associated with other metal ores, such as lead-zinc ore. The average concentration of Cd in the earth's crust is 0.15mg / Kg (Yan Mingjuan, 1999), the Cd concentration in soil is 0.01-2mg / Kg, and the Cd concentration in various water bodies is 0.11-10μg / Kg. Although under normal circumstances, Cd exists in a trace amount in the natural environment, but human activities have increased its content and distribution in the environment. For example, Cd can directly enter the atmosphere, water body and soil, and migrate in different e...

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