The invention discloses high-
cadmium-adsorption filamentous fungi
penicillium chrysogenum J-5 as well as a preparation method and an application thereof. The preparation method comprises the following steps of: firstly weighing and putting 10g of
cadmium polluted soil into 90mL of sterilized normal
saline, vibrating on a constant-temperature shaking table, standing, taking supernatant liquid and diluting; secondly
coating different
dilution factors of
diluent on a Martin rose-bengal flat plate containing 10mM of
cadmium, and inversely placing the flat plate in a constant-temperature
incubator for cultivation; thirdly, carrying out streaking and purification on microorganisms which grow on the flat plate, and then
coating the organisms on the Martin rose-bengal flat plate containing cadmium again for cultivation; fourthly, after isolation and screening, storing a
bacterial strain having stable resistance capacity on cadmium on a PDA (
Potato Dextrose Agar) inclined plane. The obtained filamentous fungi
penicillium chrysogenum J-5 has high tolerance and high adsorption property on multiple metals; the raw materials are easy to obtain and low in cost. The
bacterial strain has relatively high fixation capacity on
heavy metals in environments, can be used of reducing the biological toxic property of the
heavy metals and is suitable for the in-situ remediation of large-area polluted soil.