Epicoccum nigrum FXZ2 and application thereof

A technology of E. niger and FXZ2, which is applied in the field of environmental governance, can solve problems such as no application reports of E. niger, and achieve the effects of strong tolerance, broad application prospects, and strong selectivity

Active Publication Date: 2017-04-26
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Endophytic fungi are also commonly found in plants in heavy metal-polluted areas. At the same time, because the heavy metal content in these plants is generally high, the isolated endophytic bacteria generally have specific he

Method used

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  • Epicoccum nigrum FXZ2 and application thereof
  • Epicoccum nigrum FXZ2 and application thereof
  • Epicoccum nigrum FXZ2 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028]Embodiment 1: Screening, classification and identification of Eperococcus nigricans FXZ2

[0029] A. Collect plant samples of A. thaliana florets grown in heavy metal polluted areas, and rinse them under tap water;

[0030] B. The plant sample is divided into root, stem and leaf for surface disinfection. First, soak it in 75% ethanol for 2 minutes, rinse it with sterile water for 3 times, and then soak it in sodium hypochlorite with an available chlorine concentration of 5% for 2 minutes. Rinse with sterile water 3-5 times, and blot the surface moisture with filter paper. Cut the root, stem, and leaf tissue pieces into 2×2mm fragments and stick them on the PDA medium containing 0.5g / L streptomycin sulfate and penicillin, culture at 25°C for 45 days, observe every other day, see around the tissue pieces If there are bacterial colonies growing out, the endophytic fungal strains of the plant are obtained after picking, separating and purifying;

[0031] C. Inoculate the i...

Embodiment 2

[0037] Example 2: Detection of the tolerance level of E. nigerigera FXZ2 to different heavy metals

[0038] After the screened E. niger FXZ2 was activated on the PDA plate, they were inoculated on the PDA plate containing different heavy metal concentration gradients (Pb, Zn: 1 mmol / L, 10 mmol / L, 20 mmol / L, 30 mmol / L ,...80 mmol / L; Cd: 1mmol / L, 10 mmol / L, 20 mmol / L, 30 mmol / L,...220 mmol / L), after culturing at 25 ℃ for 7 days, it was observed that the strains were different Growth on heavy metal content PDA plates (see figure 1 , 2 , 3), and the growth on the PDA plate without heavy metals was used as the control, and each group was set up three times to obtain the minimum inhibitory concentration of each heavy metal on the strain (the lowest concentration that can inhibit the growth of the strain).

[0039] The minimum inhibitory concentrations of different heavy metals to E. nigeri FXZ2 were: cadmium (Cd 2+ ) 220 mmol / L, zinc (Zn 2 + ) 80 mmol / L, lead (Pb 2+ ) 80 mmol...

Embodiment 3

[0040] Embodiment 3: the adsorption of Eperococcus nigricans FXZ2 to heavy metal cadmium polluted water body, its reaction process is:

[0041] A. Inoculate E. niger FXZ2 on a PDA plate, culture for 3-6 days, scrape the mycelium and transfer it to potato dextrose liquid medium (PDB), 130-150r / min, 28°C for 3-6 days ;

[0042] B. Transfer the liquid medium to a 50mL centrifuge tube, centrifuge at 7000r / min for 10min to obtain mycelium. Rinse with sterile water 3-5 times, centrifuge at 7000r / min for 10min to obtain live mycelia;

[0043] C. Take a part from the living bacteria to remove the water as much as possible, put it in an oven at 80°C and dry it to a constant weight to measure the water content of the bacteria, and grind the dried dead bacteria into powder for later use;

[0044] D. Use two kinds of bacteria in different biologically active states to conduct adsorption experiments on cadmium-containing water bodies. The adsorption system is set to cadmium solution pH6....

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Abstract

The invention discloses filamentous fungi Epicoccum nigrum FXZ2 which is collected at a China General Microbiological Culture Collection Center. The collection number of the Epicoccum nigrum FXZ2 is CGMCC NO. 13573. The strain is applied to biological remediation of heavy metal lead and cadmium pollution. Experiments prove that the Epicoccum nigrum FXZ2 has high tolerance and adsorptivity for lead and cadmium, the cadmium adsorption capacity of phage of the strain is higher than that of active bacteria, the lead adsorption capacity of the active bacteria is higher than that of the phage, and the strain can remarkably promote plant growth under stress of heavy metal pollution, has good accumulation capacity for cadmium and is suitable for in-situ remediation of large-area heavy metal cadmium polluted soil.

Description

technical field [0001] The invention belongs to the field of environmental management, and in particular relates to a kind of Epicoccus nigricans ( Epicoccum nigrum ) Application of FXZ2 in bioremediation of heavy metal lead and cadmium pollution. Background technique [0002] With the development of science and technology and the continuous improvement of people's living standards, heavy metal pollution in the ecosystem is becoming more and more serious, and the treatment of heavy metal pollution has become a major environmental problem that needs to be solved urgently. Heavy metals that pollute the environment are mainly arsenic, cadmium, chromium, copper, nickel, silver, vanadium, zinc, lead, and mercury (Ahmed et al., 2015). There are two sources of them: one is naturally occurring heavy metals, which are generally not It will pollute the environment; the other is artificially produced, which is the main cause of heavy metal pollution in the environment. Heavy metals p...

Claims

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Application Information

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IPC IPC(8): C12N1/14C02F3/34B09C1/10C12R1/645C02F101/20
CPCB09C1/105C02F3/347C02F2101/20C12N1/145C12R2001/645
Inventor 李海燕吴光丽李欣亚李伟初龙孙伟宏白维晓
Owner KUNMING UNIV OF SCI & TECH
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