Screening method of chlamydomonas reinhardtii heavy metal cadmium adsorption mutants

A screening method, the technology of Rhine Clothes, is applied in the field of screening of heavy metal cadmium adsorption mutants of Chlamydomonas reinhardtii, which can solve the problems of polluted soil, secondary pollution, etc., and achieve the effect of simple steps and high screening efficiency

Inactive Publication Date: 2017-07-21
JIANGHAN UNIVERSITY
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Problems solved by technology

In addition, some cadmium-containing chemical fertilizers used in farmland will also pollute the soil
In the prior art, physical and chemical methods are usually used to treat cadm...

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  • Screening method of chlamydomonas reinhardtii heavy metal cadmium adsorption mutants
  • Screening method of chlamydomonas reinhardtii heavy metal cadmium adsorption mutants
  • Screening method of chlamydomonas reinhardtii heavy metal cadmium adsorption mutants

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Embodiment Construction

[0051] In order to better understand the above technical solution, the above technical solution will be described in detail below in conjunction with the accompanying drawings and specific embodiments.

[0052] The application provides a screening method for Chlamydomonas reinhardtii heavy metal cadmium adsorption mutants, please refer to figure 1 , the screening method includes:

[0053] Step S100, according to the influence of different cadmium chloride concentrations on the growth of Chlamydomonas reinhardtii, select a suitable screening concentration for heavy metal cadmium adsorption mutants;

[0054] Specifically, in the step S100, according to the influence of different cadmium chloride concentrations on the growth of Chlamydomonas reinhardtii, an appropriate screening concentration of heavy metal cadmium adsorption mutants is selected, including:

[0055] Step S110, cultivating Chlamydomonas reinhardtii;

[0056] The cultivation of Chlamydomonas reinhardtii described...

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Abstract

The invention discloses a screening method for heavy metal cadmium adsorption mutants of Chlamydomonas reinhardtii. The screening method comprises: selecting a suitable screening concentration for heavy metal cadmium adsorption mutants according to the influence of different cadmium chloride concentrations on the growth of Chlamydomonas reinhardtii; Transformation to obtain Chlamydomonas reinhardtii mutants; screening out heavy metal cadmium adsorption mutants of Chlamydomonas reinhardtii, the screening of heavy metal cadmium adsorption mutants of Chlamydomonas reinhardtii includes screening heavy metal cadmium sensitive mutants and screening heavy metal cadmium resistant mutants; identification screening Mutant genes of heavy metal cadmium adsorption mutants of Chlamydomonas reinhardtii. The invention achieves the technical effect of being able to obtain a large amount of Chlamydomonas heavy metal cadmium adsorption mutants simply and efficiently.

Description

technical field [0001] The invention relates to the field of biological technology, in particular to a screening method for heavy metal cadmium adsorption mutants of Chlamydomonas reinhardtii. Background technique [0002] With the continuous development of heavy industrialization, the domestic heavy metal pollution problem is also increasing. After a large amount of heavy metals enter the atmosphere, water, and soil, it will break the natural ecological balance. At the same time, through the water cycle, atmospheric cycle, and food chain, etc., heavy metals will eventually accumulate in our human body, threatening human health. Heavy metal pollution is different from other environmental pollutants. Heavy metal pollution is difficult to degrade naturally in the environment. Therefore, how to enrich and recover heavy metals from the environment is the key to solving the problem of heavy metal pollution. [0003] Among the heavy metal pollution, cadmium pollution is the most...

Claims

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Application Information

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IPC IPC(8): C12N15/87C12Q1/68C12R1/89
CPCC12N15/87C12Q1/6806C12Q1/6895C12Q2600/156
Inventor 陈斌李丽丽胡长峰
Owner JIANGHAN UNIVERSITY
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