The application relates to the field of
water quality detection, in particular to a paper-based
nitrate ion detection
chip and a preparation method and application thereof. The paper-based
nitrate ion detection
chip comprises a bottom plate and a paper-based layer on the bottom plate; the paper-based layer comprises a color developing area, a reduction area and a sample inlet area which are sequentially communicated; a detection
reagent is pre-fixed in the color developing area, the detection
reagent comprises glucose, p-aminobenzenesulfonic acid, N-1-naphthyl
ethylenediamine and
ascorbic acid; a reduction
reagent is pre-fixed in the reduction area, the reduction reagent comprises
zinc powder and
cadmium chloride; the detection reagent solution is added to the color developing area and then freeze-dried, so that the detection reagent is pre-fixed in the color developing area. The freeze-
drying technology is used to uniformly pre-fix the detection reagent on the
filter paper, a loose structure is formed, the color developing uniformity of the detection reagent after reacting with
nitrate ions can be effectively improved, and the reaction time is reduced.