5 results about "Manganous chloride" patented technology

This invention provides a method for producing PD-1 antibodies with high protein expression levels, high antibody activity, and / or high galactosyl levels. This method involves culturing cells in a culture medium until the target protein is obtained. The culture temperature is 37°C, which is then lowered to 33-34°C on the 5th day of culture. The culture medium also contains additives, namely galactose and manganese chloride. The concentrations of galactose and manganese chloride added are 0.05-6 mM and 0.05-3 μM, respectively. The culture medium consists of a basal medium and a feed medium. The basal medium is Star CHO, and the feed medium is Star CHO Feed and CDFS36. The basal medium also contains 1 g / L poloxamer. The Star CHO Feed feed is added at 4% / 5% / 6% / 6% / 5% / 4% of the initial culture volume each time, and the CDFS36 feed is added at 0.4% / 0.5% / 0.6% / 0.6% / 0.5% / 0.4% of the initial culture volume each time. The additives galactose and manganese chloride, as well as the Star CHO feed feed... Feed and CDFS36 were added every 2 days starting from day 3 of culture; the dissolved oxygen in the culture system was 40%, and the pH was 7.0 ± 0.1; the rotation speed of the culture system was 240 rpm.

This invention relates to the field of biomedical technology and discloses a method for preparing and applying copper-manganese coordinated taurine-Schiff base nanopolymers. The preparation method includes: dissolving taurine and an equimolar amount of sodium hydroxide in anhydrous ethanol, heating under reflux, and adding protocatechuic aldehyde dissolved in anhydrous ethanol dropwise while stirring; heating under reflux and stirring; a yellow solid precipitates from the reaction solution, which is then transferred to a refrigerator to further precipitate the solid, and collected to obtain taurine-Schiff base; dissolving copper chloride and manganese chloride in an aqueous solution of polyvinylpyrrolidone, adding taurine-Schiff base while stirring, adjusting the pH of the reaction solution to alkaline with an alkaline solution, stirring, and collecting to obtain copper-manganese coordinated taurine-Schiff base nanopolymers; ultrasonically dispersing the nanopolymer in ultrapure water, adding an anti-angiogenic drug, stirring, and collecting to obtain drug-loaded copper-manganese coordinated taurine-Schiff base nanopolymers. This invention achieves effective local treatment and immune system activation, while improving treatment safety.

A method for preparing biomimetic nanocomposite materials based on thylakoid membranes is disclosed, belonging to the field of bionanomaterials technology. This invention addresses the problems of existing nano-manganese oxide, which is easily cleared by the immune system, has difficulty penetrating complex tumor matrixes, and suffers from complex synthesis methods and high costs; it also addresses the issues of insufficient rigidity, instability, inability to accurately reach tumor sites, and limited functionality in existing single-membrane structures. The method comprises: 1. Dispersing a thylakoid membrane in pure water, then heating and adding hydrochloric acid solution to obtain a Mg-removed Nks system; 2. Adding an aqueous manganese chloride solution to the Mg-removed Nks system, then adjusting the pH and continuing stirring. This invention is used for the preparation of biomimetic nanocomposite materials based on thylakoid membranes.

The application provides a method for recycling seabed cobalt-manganese oxide ore hydrometallurgy slag, and relates to the field of metallurgical industry. The method comprises the following steps: providing seabed cobalt-manganese oxide ore hydrometallurgy slag; mixing, reacting the seabed cobalt-manganese oxide ore hydrometallurgy slag, carbon monoxide and chlorine gas to obtain reaction slag, and collecting flue gas in the reaction process; condensing and crystallizing the flue gas to obtain iron chloride; washing the reaction slag by using an ether compound, and performing solid-liquid separation to obtain washing slag; performing water immersion and solid-liquid separation on the washing slag to obtain a manganese chloride solution. The iron and manganese extraction rate of the method is greater than 95%, the products formed by iron and manganese are different in boiling point and vapor pressure, and the products are separated step by step by controlling the reaction conditions, so that the problem that the products cannot be separated does not exist; the product collection process is simple, the iron product can be quickly collected by flue gas condensation, the reaction slag is washed to retain manganese chloride, and the product can be collected by dissolution and evaporation crystallization.

The application discloses a magnetic nanometer probe with small size and high magnetic particle imaging performance, and a preparation method and application thereof. In the application, iron chloride is dissolved in a glycol solution, and a uniform transparent solution is formed through magnetic stirring; manganese chloride with different concentrations is added into the above solution, and stirring and dissolution are carried out; sodium acetate is added, and uniform stirring is carried out to form a transparent solution; finally, sodium citrate is dissolved in the above solution, uniform stirring is carried out, and the mixed solution is reacted through a solvothermal method; after the reaction is completed, the reaction product is purified through centrifugation and dialysis; finally, the supernatant is collected and concentrated, and the magnetic nanometer probe is obtained. The magnetic nanometer probe prepared through the method has small size, high sensitive imaging performance and excellent hydrophilic performance, and is expected to be used for the diagnosis of micro lesions.