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Solid-state culture medium for benefitting long-term storage of haematococcus pluvialis algae and preparation method thereof

A technology of Haematococcus pluvialis and solid medium, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., which can solve the problems of prolonging the storage time of Haematococcus pluvialis species, easily polluting bacteria, excessive, etc. problems, to achieve the effect of convenient and quick microalgae breeding and inhibiting the growth of bacteria

Inactive Publication Date: 2016-08-31
GUANGDONG HAIRONG ENVIRONMENTAL PROTECTION TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a new formula of Haematococcus pluvialis solid-state seed preservation medium, which solves the disadvantages of inhibiting the reproduction of Haematococcus pluvialls, insufficient or excessive growth factors, and easy contamination of bacteria, etc. Effect of storage time of Haematococcus species

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] A kind of preparation method that is beneficial to the solid-state culture medium of long-term storage Haematococcus pluvialis species, comprises the following steps:

[0015] Step 1: Measure 1 liter of ultrapure water;

[0016] Step 2: 1.4g of sodium nitrate; 0.13g of sodium carbonate; 0.25g of magnesium sulfate; 0.05g of dipotassium hydrogen phosphate; 0.019g of ferric ammonium citrate; Sodium 0.010g; Zinc sulfate 0.000222g; Boric acid 0.00586g Manganese chloride 0.0018g; Cobalt nitrate 0.000894g; Sodium molybdate 0.00039g; Copper sulfate 0.000150g; 5ml; Vitamin B12 5ml was added to ultrapure water, and each component was dissolved;

[0017] Step 3: Add the solution obtained in step 2 into an autoclave, sterilize at 120°C for 20 minutes, and then store it in a sterile environment at a storage temperature of 40°C.

Embodiment 2

[0019] A kind of preparation method that is beneficial to the solid-state culture medium of long-term storage Haematococcus pluvialis species, comprises the following steps:

[0020] Step 1: Measure 1 liter of ultrapure water;

[0021] Step 2: 1.35g of sodium nitrate; 0.14g of sodium carbonate; 0.2g of magnesium sulfate; 0.08g of dipotassium hydrogen phosphate; 0.014g of ferric ammonium citrate; Sodium 0.005g; Zinc sulfate 0.0007g; Boric acid 0.0045g Manganese chloride 0.0028g; Cobalt nitrate 0.0006g; Sodium molybdate 0.0006g; Copper sulfate 0.00012g; Sodium acetate 0.8g; Agar powder 16g; 4ml; vitamin B12 6ml was added to ultrapure water, and each component was dissolved;

[0022] Step 3: Add the solution obtained in step 2 into an autoclave, sterilize at 120°C for 20 minutes, and then store it in a sterile environment at a storage temperature of 40°C.

Embodiment 3

[0024] A kind of preparation method that is beneficial to the solid-state culture medium of long-term storage Haematococcus pluvialis species, comprises the following steps:

[0025] Step 1: Measure 1 liter of ultrapure water;

[0026] Step 2: 1.3g of sodium nitrate; 0.15g of sodium carbonate; 0.15g of magnesium sulfate; 0.10g of dipotassium hydrogen phosphate; 0.009g of ferric ammonium citrate; Sodium 0.001g; zinc sulfate 0.001000g; boric acid 0.00286g manganese chloride 0.0036g; cobalt nitrate 0.000494g; sodium molybdate 0.00089g; copper sulfate 0.000079g; sodium acetate 1.0g; agar powder 15g; 2ml; vitamin B12 10ml was added to ultrapure water, and each component was dissolved;

[0027] Step 3: Add the solution obtained in step 2 into an autoclave, sterilize at 120°C for 20 minutes, and then store it in a sterile environment at a storage temperature of 40°C.

[0028] Examples 1-3 provide sufficient nitrogen source, phosphorus source, and carbon source to provide sufficient...

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Abstract

The invention discloses a solid-state culture medium for benefiting long-term storage of haematococcus pluvialis algae. The solid-state culture medium is characterized by being prepared from the following components in every liter of the culture medium: 1.3-1.4g of sodium nitrate, 0.13-0.15g of sodium carbonate, 0.15-0.25g of magnesium sulfate, 0.05-0.10g of dipotassium phosphate, 0.009-0.019g of ammonium ferric citrate, 0.010-0.050g of citric acid, 0.025-0.035g of potassium sorbate, 0.001-0.010g of ethylene diamine tetraacetic acid, 0.000222-0.001000g of zinc sulfate, 0.00286-0.00586g of 0.00286-0.00586g of boric acid, 0.0018-0.0036g of manganese chloride, 0.000494-0.000894g of cobalt nitrate, 0.00039-0.00089g of sodium molybdate, 0.000079-0.000150g of copper sulfate, 0.1-1.0g of sodium acetate, 15-17g of agar powder, 0.025-0.050g of biotin, 2-5ml of vitamin B1 and 5-10ml of vitamin B12. The invention aims to provide a novel formula and the preparation method of the haematococcus pluvialis solid-state seed-conservation culture medium to solve the problems that reproduction of haematococcus pluvialis is inhibited by the existing formula, growth factors are not sufficient or excessive and are easily polluted by bacteria, and the like, so that the effect of prolonging the preservation period of haematococcus pluvialis algae can be achieved.

Description

technical field [0001] The invention relates to the technical field of medium formulations, in particular to a solid-state medium beneficial to long-term storage of Haematococcus pluvialis species, and a preparation method of the medium. Background technique [0002] Haematococcus pluvialis is a single red cell green algae living in fresh water, also known as Haematococcus lacustrine or Haematococcus lacustrine, is a ubiquitous green algae, which can accumulate a large amount of astaxanthin and It is red, so it is called Haematococcus, because it is a pluviall species in green algae, also known as Haematococcus pluvialis. Astaxanthin in Haematococcus pluvialis is a kind of carotenoid, and it is also the highest-level product of carotenoid synthesis found so far. It has the strongest antioxidant activity in nature, so it is known as the anti-oxidant in the 21st century. Revolutionary product. The aging of the human body is mainly due to the oxidation caused by free radicals...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12R1/89
CPCC12N1/12
Inventor 闫潍皓史会帅
Owner GUANGDONG HAIRONG ENVIRONMENTAL PROTECTION TECH CO LTD
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