177 results about "Guanidinium chloride" patented technology

An improved method of preserving a molecule in a bodily fluid comprises: (1) providing a preservative solution comprising: (a) an amount of a divalent metal chelator selected from the group consisting of ethylenediaminetetraacetic acid (EDTA), (ethylenebis(oxyethylenenitrilo))tetraacetic acid (EGTA), and 1,2-bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid (BAPTA) and salts thereof in the range of from about 0.001 M to about 2 M; and (b) an amount of at least one chelator enhancing component selected from the group consisting of lithium chloride, guanidinium chloride, guanidinium thiocyanate, sodium salicylate, sodium perchlorate, and sodium thiocyanate in the range of from about 0.1 M to about 10 M; and (2) adding the preservative solution to the bodily fluid, thus preserving the molecule. The molecule can be a protein or a small molecule, such as a steroid. The invention also encompasses preservative compositions suitable for preserving proteins or small molecules, and kits. Preservative compositions can further include at least one enzyme inactivating component selected from the group consisting of manganese chloride, sarkosyl, and sodium dodecyl sulfate in the range of up to about 5% molar concentration. Compositions and methods according to the present invention have many diagnostic and forensic uses, in addition to being suitable for preparing compositions usable by hunters for attracting animals.

The invention discloses a preparation method for non-denatured II type collagen of an animal cartilage source. The preparation method is characterized by comprising the following steps: taking fresh and traceable animal cartilages as raw materials; carrying out purification treatment on the animal cartilages by virtue of processes of repeated freeze-thawing, ultrasonic degreasing, hypertonic solution decellularizing, acid-base softening and the like; carrying out processes of guanidine hydrochloride sugar-removing, pickling, compound enzyme treatment, multi-time salting-out, multi-time centrifugal purification, membrane dialysis, freeze-drying and the like, thereby obtaining spongy non-denatured II type collagen. The high-purity high-yield II type collagen obtained by the preparation method keeps the original complete triple-helix structure of the collagen, is high in biological activity, stable in structure, easy to store and beneficial to adhesion, growth and multiplication of cartilage cells, and can be used for preparing medical biological materials.

The invention relates to a construction method of a medical titanium alloy implant surface growth factor delivery system, which comprises the following steps that: micro-groove ridges are prepared on the surface of a medical low-elastic Beta titanium alloy; gelatin is dissolved into deionized water, and then 2-imine hydrogen chloride mercaptan is added to be dialyzed and dried, to prepare SH gelatin; the SH gelatin is dissolved in double-distilled water to be ultrasonically homogenized to prepare B liquid, emulsifier is added into liquid paraffin, and then the B liquid is dripped into the liquid paraffin which is added with the emulsifier, isopropyl alcohol and ether are cleaned, added with glutaraldehyde and cured, ether elutes, dries and sieves out the cured substance, and 60Co irradiates and sterilizes, to prepare SH gelatin microspheres; and the microspheres are soaked into rhBMP2 guanidine hydrochloride saturated solution and freeze dried under a vacuum condition, to prepare the SH gelatin microspheres loaded with growth factors. Medical low-elastic Beta titanium alloy implant material is soaked into dopamine solution, and a layer of adhered polymer thin film is generated on the surface of the titanium alloy; and the implant material which decorates the surface is put into the growth factor SH gelatin microsphere solution, vacuum is pumped, and the medical titanium alloy implant surface growth factor delivery system is prepared.

The present invention relates to the solubilization and recovery in high yield, of inclusion body proteins from host cells using an appropriate denaturating agent. The process avoids the use of high concentration of chaotropic agents such as guanidine hydrochloride or urea.

The invention discloses a preparation method of a spherical N-doped C-coated metal oxide negative electrode material with a multi-stage structure and belongs to the field of lithium ion batteries. Thepreparation method comprises the following specific steps: mixing and stirring N-methylpyrrolidone, polyvinylidene fluoride and guanidine hydrochloride to transparent gel, drying to remove a solventand calcining in argon to obtain N-doped C; dissolving sodium molybdate, nickel nitrate, ammonium fluoride and urea in an alcohol solution, stirring into a homogeneous solution and performing hydrothermal reaction; then mixing the N-doped C and the homogeneous solution, ball-milling and calcining to obtain a NiO/NiMoO4@N-C material; putting the NiO/NiMoO4@N-C material in distilled water and addingsodium dodecyl sulfate after ultrasonic treatment; adding pyrrole and hydrochloric acid, stirring, then adding an initiating agent, centrifuging, washing and drying to obtain. The negative electrodematerial synthesized by the preparation method disclosed by the invention is uniform and consistent in particle, good in dispersibility and high in degree of crystallinity and has the stable multi-stage composite structure, thereby having appreciable wide potential window reversible capacity, excellent rate capability and stable cycle life.

The invention discloses a 4'-pyridylpyrimidine compound and a synthetic method and application thereof. By using a derivative iso-longitolanone of natural renewable resource turpentine as a raw material, a novel 4'-pyridylpyrimidine compound is prepared. Iso-longitolanone and 4-pyridylaldehyde are subjected to condensation to generate 7-(pyridine-4'-yl-methylene)iso-longitolanone; 7-(pyridine-4'-yl-methylene)iso-longitolanone and guanidine hydrochloride are subjected to condensation and cyclization to obtain a 4'-pyridylpyrimidine compound 6,6,10,10-tetramethyl-4-(pyridine-4'-yl)-5,7,8,9,10,10a-hexahydro-6H-6a,9-methanobenzo-2-quinazolinamine. The compound can specifically recognize copper ion, can be specifically complexed with Cu<2+> ion and generates quenching of blue fluorescence. Thus, the compound can be used as a fluorescence probe for detection of copper ion and has good practicality.

The application discloses a water-treated polyhexamethylene guanidine hydrochloride sterilizing agent and a preparation method thereof. The water-treated polyhexamethylene guanidine hydrochloride sterilizing agent is prepared by weighing and uniformly mixing guanidine hydrochloride, hexamethylene diamine, hexamethylenediamine, polyethylene glycol, water, methylbenzene, a polymerization inhibitor, sulphonic acid, maleic anhydride, N-t-butyloxycarboryl-1,6-diamino hexane, a dispersing agent, ferric sulfate, methacrylic acid, dodecyl dimethyl benzyl ammonium chloride and methyl acrylate according to parts by weight. The water-treated polyhexamethylene guanidine hydrochloride sterilizing agent is 100% soluble in water, has no volatility, can be completely neutralized with soaps, and has no pollution to environment; the integral of an acute skin irritancy test is 0, and the water-treated polyhexamethylene guanidine hydrochloride sterilizing agent is colorless, non-poisonous, odorless and tasteless, and has no corrosion to metal, rubber and plastic; the sterilizing rate to escherichia coli, staphylococcus aureus, candida albicans, neisseria gonorrhoeae, penicillium, aspergillus, saprophytic bacteria and salmonella is 100%, when the water-treated polyhexamethylene guanidine hydrochloride sterilizing agent is placed at 50-60 DEG C for a month, the sterilizing rate is 98-100%, when the water-treated polyhexamethylene guanidine hydrochloride sterilizing agent is placed at 40-50 DEG C for half a year, the sterilizing rate is 97.5-99.5%, and when the water-treated polyhexamethylene guanidine hydrochloride sterilizing agent is placed at the room temperature for 1-2 years, the sterilizing rate is 95-99%.

The invention relates to a method for preparing 2-amino-4,6-dichloro-5-formamido pyrimidine. The method comprises the following steps of: performing nitrosation on malonic acid diethyl ester and acetic acid serving as raw materials and sodium nitrite at first; then, performing reduction and formylation with formic acid in the presence of zinc powder to form formyl amino malonic acid diethyl ester; finally, performing condensation and cyclization with guanidine hydrochloride to produce 2-amino-4,6-dichloro-5-formamido pyrimidine; performing chlorination by using quaternary ammonium salt as a catalyst; fractionally performing hydrolysis under an alkali action to obtain a product. The method has easily-available raw materials, and is short in reaction time, simple in aftertreatment and high in hydrolysis selectivity; the cost is obviously reduced; the total yield is up to 74 percent and the purity of the product is up to 99.0 percent.

The invention relates to a preparation method of 1, 3-diaminoguanidine monohydrochloride. The preparation method comprises the following steps of 1, dissolving guanidine hydrochloride as a raw material in water, adding a proper amount of toluene into the solution, dropwisely adding hydrazine hydrate into the mixed solution to cause a reaction lasting for 5-10h at a temperature of 50-110 DEG C, then adjusting a pH value to less than 3 by hydrochloric acid, carrying out standing liquid-separation, taking a water layer, and carrying out distillation concentration until the water layer produces a trace amount of precipitates, wherein a mass ratio of guanidine hydrochloride to water is 1: (2-6), a mass ratio of water to toluene is 1: (0.2-0.5) and a mole ratio of guanidine hydrochloride to hydrazine hydrate is 1: (1.9-2.5), and 2, slowly and dropwisely adding the concentrated solution obtained by the step 1 into a reactor with anhydrous ethanol along with stirring so that crystals are produced, washing the white crystals by cool water with a temperature of 1-6 DEG C and mass 2-3 times that of the concentrated solution, and carrying out drying to obtain a white solid object product, wherein a mass ratio of the concentrated solution to the anhydrous ethanol is 1: (3-5). The preparation method has the advantages of simple processes, mild conditions, high yield, high purity and environmental friendliness.

The invention discloses camphoryl pyrimidines, and a preparation method and an anti-tumor activity study thereof. With camphor as a raw material and under an alkaline catalysis condition, a series ofalpha,beta-unsaturated ketones are obtained through aldol condensation reaction of camphor with different aromatic aldehydes respectively, a series of camphoryl pyrimidines are obtained through annulation reaction of alpha,beta-unsaturated ketones with guanidine hydrochloride through catalysis of potassium tert-butoxide, and the anti-tumor activity of the synthesized camphoryl pyrimidines is studied. Experiments show that the camphoryl pyrimidines have good inhibitory activity on human multiple myeloma cells (RPMI-8226), human breast cancer cells (MDA-MB-231) and human non-small cell lung cancer cells (A549), have less toxicity on normal cell human gastric mucosa cells (GES-1), and have potential anti-tumor application value.

The present invention relates to a solid phase immunoassay comprising on said solid phase an antigen in the presence of a reducing agent. The present invention also relates to a method for purifying a cysteine containing recombinantly expressed protein comprising at least 2, preferably 3 or 4 and even more preferably all of the following steps: (a) sulphonation of a lysate from recombinant host cells or lysis of recombinant host cells in the presence of guanidinium chloride followed by a subsequent sulphonation of the cell lysate, (b) treatment with a zwitterionic detergent, preferably after removal of the cell debris, (c) purification of the sulphonated version of the recombinant protein or purification of the sulphonated version of the recombinant protein with subsequent removal of the zwitterionic detergent, with said purification being preferably chromatography, more preferably a Ni-IMAC chromatography with said recombinant protein being a His-tagged recombinant protein, (d) desulphonation of the sulphonated version of the recombinant protein, preferably with a molar excess of DTT, (e) storage in the presence of a molar excess of DTT. The present invention also relates to novel HCV NS3 sequences as depicted in FIGS. 1-8.

The invention discloses a novel wood floor adhesive. The adhesive is prepared by using the following raw materials, by weight, 5-10 parts of sodium polyacrylate, 0.5-1.7 parts of titanium dioxide, 1.5-3 parts of ethyl alpha-cyanoacrylate, 2.5-4.3 parts of alkyl polyglycoside, 1.5-2.8 parts of steam-exploded lignin, 1.2-3.6 parts of polyethylidene maleic acid, 0.5-1.2 parts of sodium diacetate and 0.9-2.2 parts of guanidine hydrochloride. Compared with present adhesives, the adhesive disclosed in the invention has the advantages of strong adhesion, no shedding, easily available raw materials, simple preparation technology, no benzene or aldehyde, non-toxicity, harmlessness, environmental protection, low carbon, high solid content, small shrinkage, good initial tack, fast drying speed, freeze resistance and easy gluing.

The invention discloses a kit for extracting blood free DNA. The kit of the invention includes a lysis solution, a binding solution, a cleaning solution 1 and a cleaning solution 2. The binding solution contains 2-5 M guanidine isothiocyanate or guanidine hydrochloride, 10-100mM Tris-HC1, 10-50mM EDTA, 5-15% Triton X-100, and 20-50% absolute ethanol or isopropanol. The cleaning solution 1 contains2-5 M guanidine isothiocyanate or guanidine hydrochloride, 10-100mM Tris-HCl, 10-50mM EDTA, 5-10% Triton X-100, SDS or Tween 20, and 20-50% absolute ethanol or isopropanol. The kit of the invention improves the binding solution and increases the extraction efficiency, improves the cleaning solution 1, increases the binding efficiency of the magnetic beads cfDNA and removes impurities, improves the recovery efficiency and purity of the cfDNA and reduces the material cost.

The invention discloses a preparation method of a functionalized modified molybdenum disulfide nanosheet. The preparation method comprises the following steps of: firstly, uniformly mixing phosphonitrilic chloride trimer and guanidine hydrochloride with a given amount of molybdenum disulfide powder through a ball milling method to prepare a molybdenum disulfide nanosheet precursor; putting the precursor into a quartz furnace, preparing an element-doped nanosheet through an annealing process; and finally modifying the surface of a molybdenum disulfide nanosheet with a cobalt boride nanosheet inan in-situ growth manner, and obtaining the functionalized modified molybdenum disulfide nanosheet. The functionalized modified molybdenum disulfide nanosheet provided by the invention is simple in preparation method, low in cost and high in surface modifier content. The prepared modified molybdenum disulfide nanosheet has multifunctionality, for example, the modified molybdenum disulfide nanosheet can be used as a novel solid lubricating material or a hybrid flame retardant with a synergistic effect. In addition, the dispersion state and compatibility of the molybdenum disulfide nanosheet ina polymer matrix is improved, so that the improvement of the comprehensive performance of the polymer material can be benefitted.

The invention belongs to the technical field of photocatalytic materials, and discloses preparation and application of a carbon dot-modified carbon nitride/tin dioxide composite photocatalyst. The photocatalyst is prepared through the following steps: calcining guanidine hydrochloride at 500-550 DEG C, and carrying out grinding to obtain carbon nitride powder; dissolving tin tetrachloride pentahydrate in ultrapure water, carrying out heating at 140-160 DEG C, and performing centrifugal cleaning to obtain tin dioxide; dissolving citric acid and urea in ultrapure water, carrying out ultrasonic treatment, conducting heating at 160-180 DEG C, performing centrifuging, collecting a supernatant, and dissolving the supernatant in deionized water to obtain a CDs stock solution; dissolving carbon nitride powder and stannic oxide in ethanol, carrying out heating in a water bath at 60-70 DEG C, calcining the obtained solid at 350-400 DEG C, dissolving the obtained CN/SnO2 and CDs stock solution inethanol, carrying out ultrasonic treatment, performing heating in a water bath at 60-70 DEG C, and calcining the obtained solid at 280-300 DEG C to obtain the photocatalyst. The photocatalyst provided by the invention effectively improves the light absorption performance of a CN/SnO2 photocatalytic material.

The invention provides a nucleic acid extraction kit for an excrement sample, a preparation method and an extraction method, and relates to the technical field of biology, the kit comprises protease K, a primary lysis buffer solution and a lysis binding buffer solution; the concentration range of the protease K is 10-30 mg/mL; the primary lysis buffer solution comprises Tris-HCl with the pH value of 6.0 to 8.550 to 200 mM, SDS with the concentration of 0.5 to 30 percent (w/v) and EDTA with the concentration of 1 to 10 mM, and the pH value of 8.0 to 9.0; the lysis binding buffer solution is prepared from 50 to 200mM of Tris-HCl, 1 to 15M of guanidine hydrochloride, 1 to 10mM of EDTA (Ethylene Diamine Tetraacetic Acid), 0.1 to 5 percent of TritonX-100 and 0.1 to 5 percent of Tween-20, and the pH (Potential of Hydrogen) of the lysis binding buffer solution is 7.0 to 8.0. The DNA nucleic acid extracted and purified by the kit is high in yield and good in purity, and the problem that the preservation time of the DNA extracted from the excrement sample is short is solved.

The invention discloses a durable, antistatic and dustproof finishing agent for flocking fabrics. The finishing agent is prepared by matching, by weight, 50-100 parts of methyl hydrogen silicone oil, 70-120 parts of allyl polyethoxy polypropoxy ether, 1.5-5 parts of vinyl trialkoxy silane and 0.1-0.16 part of chloroplatinic acid ethanol solution, making the materials subjected to addition reaction to obtain silicone oil co-modified by polyether and alkoxy, adding 480-820 parts of water for dilution and adding 10-20 parts of glyoxal aqueous solution and 400-500 parts of guanidine hydrochloride aqueous solution for mixing. The flocking fabrics finished through the finishing agent are soft and smooth in hand feel and have excellent antistatic properties, and the fabric surface is not prone to adsorb dust.

The invention relates to a method for preparing a cartilage angiogenesis inhibiting factor for a scalloped hammerhead shark. During preparation, Pro-Asp-Tyr-Lys-Phe-Lys is obtained through cartilage homogenate of the scalloped hammerhead shark, guanidine hydrochloride extraction, acetone precipitation and grading, enzymolysis, gel filtration chromatography, cell membrane chromatography purification and efficient liquid phase chromatography purification. The angiogenesis inhibiting factor can effectively inhibit angiogenesis of chick chorioallantoic membranes, and has an obvious inhibition function on expression of three angiogenesis promoting factors including the vascular endothelial cell growth factor, the basic fibroblast growth factor and the platelet-derived growth factor of lung cancer tissue of mice suffering from the Lewis lung cancer.

The invention discloses a wood floor adhesive prepared from the following raw materials in parts by weight: 5-10 parts of phenylalanine, 0.5-1.7 parts of titanium dioxide, 1.5-3 parts of trihydroxytriethylamine, 2.5-4.3 parts of fatty alcohol alkanolamide, 1.5-2.8 parts of steam explosion lignin, 1.2-3.6 parts of polyethylidene maleate, 0.5-1.2 parts of sodium diacetate and 0.9-2.2 parts of guanidine hydrochloride. Compared with the existing adhesive, the wood floor adhesive disclosed by the invention is strong in adhesive force, not easy to fall off, available in raw materials, simple in preparation process, benzene-free, formaldehyde-free, nontoxic, innocuous, environment-friendly and low-carbon and also has the advantages of high solid content, small shrinkage rate, good initial adhesiveness, high drying speed, freeze prevention, simplicity in gluing and the like.

The invention discloses a nano multidirectional chromatography nucleic acid extraction medium, which has a substrate of a macromolecular carbohydrate and an extract penetrating the substrate. The extract comprises a mixture of guanidine hydrochloride, SDS and EDTA located at a first layer, a protective agent located at a second layer, a mixture of guanidine hydrochloride, SDS and EDTA located at a third layer, and a protective agent located at a fourth layer. And the mixture of guanidine hydrochloride, SDS and EDTA located at the first layer and the mixture of guanidine hydrochloride, SDS and EDTA located at the third layer are different in content. The substrate can well preserve the nucleic acid integrity and prevent nucleic acid degradation.

The invention discloses a nucleic acid purifying reagent and method, and belongs to the biological field. The reagent contains magnetic beads, DNA binding liquid, a scrubbing solution and eluant, wherein the magnetic beads are silicon-hydroxyl-modified magnetic beads; the DNA binding liquid is guanidine hydrochloride, sodium acetate and Triton X-100; the scrubbing solution includes a reagent 1 including guanidine hydrochloride, sodium acetate and Triton X-100 as well as a reagent 2 including ethanol, sodium acetate and sodium citrate; and the eluant is Tris-HCl. By adding sodium cocoyl glutamate and sodium lauryl glutamate into the DNA binding liquid in a concentration ratio of 1 to (1-5) in an implementation process, a purification effect can be obviously improved. By virtue of interaction of the reagents, the purification efficiency of nucleic acid is obviously improved, the purity is high, and the separation purification yield can reach up to 98.76% and is obviously higher than thatin the prior art.

The invention provides a novel plant base multifunctional strong building glue and a preparation method thereof. The novel plant base multifunctional strong building glue provided by the invention is prepared from water, polyvinyl alcohol resin, soybean protein isolate, guanidine hydrochloride and sodium sulfate. The glue provided by the invention can effectively prevent generation of cracks, and is a completely green environment-friendly product, which has advantages of simple and convenient preparation method, low cost and wide sources of materials.