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Method for preparing cartilage angiogenesis inhibiting factor for scalloped hammerhead shark

An angiogenesis-inhibiting, scalloped-hammer shark technology is applied in the field of preparation of scalloped-hammer shark cartilage angiogenesis-inhibiting factor, which can solve the problems that the application of scalloped-hammer shark cartilage-angiogenesis-inhibiting factor has not been reported, and achieves Significant activity and advanced purification process

Active Publication Date: 2015-09-09
ZHEJIANG OCEAN UNIV
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Problems solved by technology

[0005] The applicant's research found that the research on the preparation of angiogenesis inhibitory factors using scalloped hammerhead cartilage as raw material is in a blank stage, and the application of scalloped hammerhead cartilage angiogenesis inhibitors in the preparation of drugs for inhibiting angiogenesis and preventing and treating tumors It has not been reported

Method used

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  • Method for preparing cartilage angiogenesis inhibiting factor for scalloped hammerhead shark
  • Method for preparing cartilage angiogenesis inhibiting factor for scalloped hammerhead shark
  • Method for preparing cartilage angiogenesis inhibiting factor for scalloped hammerhead shark

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Embodiment

[0025] A method for preparing angiogenesis inhibitory factor of scalloped hammerhead shark, the preparation process is as follows: cartilage of scalloped hammerhead shark → tissue crushing → guanidine hydrochloride extraction → fractional precipitation with acetone → purification by gel filtration chromatography → purification by cell membrane chromatography → high-efficiency Liquid chromatography purification → angiogenesis inhibitory factor.

[0026] 1) Preparation of active protein components of hammerhead shark cartilage: The crushed and homogenized scalloped hammerhead shark ( Sphyrna lewini ) Cartilage was added to 1.0 mol / L guanidine hydrochloride solution at a solid-to-liquid ratio of 1 g: 10 mL, extracted by shaking at 4 °C for 36 h, centrifuged at 4 °C and 10 000 r / min for 20 min, and the supernatant was taken into a dialysis bag with a molecular weight cut-off of 1 kDa, and dialyzed with Tris-HCl (pH 7.6) buffer solution at below 4°C for 24 h to obtain the crude e...

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Abstract

The invention relates to a method for preparing a cartilage angiogenesis inhibiting factor for a scalloped hammerhead shark. During preparation, Pro-Asp-Tyr-Lys-Phe-Lys is obtained through cartilage homogenate of the scalloped hammerhead shark, guanidine hydrochloride extraction, acetone precipitation and grading, enzymolysis, gel filtration chromatography, cell membrane chromatography purification and efficient liquid phase chromatography purification. The angiogenesis inhibiting factor can effectively inhibit angiogenesis of chick chorioallantoic membranes, and has an obvious inhibition function on expression of three angiogenesis promoting factors including the vascular endothelial cell growth factor, the basic fibroblast growth factor and the platelet-derived growth factor of lung cancer tissue of mice suffering from the Lewis lung cancer.

Description

[0001] Preparation method of scalloped hammerhead cartilage angiogenesis inhibitory factor technical field [0002] The invention relates to a preparation method of angiogenesis inhibitory factor from aquatic organisms, in particular to a preparation method of scalloped hammerhead cartilage angiogenesis inhibitory factor. Background technique [0003] Angiogenesis is one of the important pathological features of vascular proliferative diseases such as tumors, diabetic retinopathy, rheumatoid arthritis and chronic inflammation. The anti-angiogenesis-based tumor biotherapy research has become a research hotspot of anti-tumor drugs in the past ten years. [0004] Compared with chemotherapy aimed at directly killing tumor cells, tumor angiogenesis inhibitor (TAI) has the following unique advantages: (1) TAI directly acts on vascular endothelial cells, while anticancer drugs diffuse through tissues Affected by tissue fibrosis, necrosis, etc., the effective drug concentration i...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/20C07K1/16
Inventor 王斌迟长凤赵玉勤孙坤来
Owner ZHEJIANG OCEAN UNIV
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