Preparation method and use of mustelus griseus cartilage blood vessel generation inhibiting factor

An angiogenesis inhibition and gray star shark technology, applied to cardiovascular system diseases, medical preparations containing active ingredients, pharmaceutical formulas, etc., to achieve significant activity, easy operation, and simple purification process

Inactive Publication Date: 2015-01-28
ZHEJIANG OCEAN UNIV
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Problems solved by technology

[0005] The applicant's research found that the research on the preparation of angiogenesis inhibitors using gray star shark cartilage as raw material is in a blank stage, and the application of the gray star shark cartilage inhibitor of angiogenesis BSFN-I in the preparation of drugs that inhibit angiogenesis and prevent and treat tumors is even better. is unreported

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  • Preparation method and use of mustelus griseus cartilage blood vessel generation inhibiting factor
  • Preparation method and use of mustelus griseus cartilage blood vessel generation inhibiting factor
  • Preparation method and use of mustelus griseus cartilage blood vessel generation inhibiting factor

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Embodiment Construction

[0021] The present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments.

[0022] Embodiment: a kind of preparation method of gray star shark angiogenesis inhibitory factor BSFN-I, preparation process is as follows: gray star shark cartilage "tissue breakage" guanidine hydrochloride extraction "acetone fractionation precipitation "ion exchange chromatography purification" gel permeation Chromatographic purification of "angiogenesis inhibitory factor BSFN-I.

[0023] 1) Extraction of the crude extract of gray star shark cartilage protein: crush and homogenate the gray star shark cartilage, add it to the guanidine hydrochloride solution with a concentration of 1.0 mol / L at a solid-liquid ratio of 1 g: 8 mL, shake and pump at below 4 °C After extraction for 24 h, the extracted solution was centrifuged below 4 °C at 5 000 r / min for 15 min to remove the residue and collect the supernatant; the supernatant was centrifuged ...

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Abstract

The invention relates to a preparation method and use of a mustelus griseus cartilage blood vessel generation inhibiting factor BSFN-I. An N tail end of the mustelus griseus cartilage blood vessel generation inhibiting factor comprises 15 amino acid residues of Asp-Pro-Gly-Thr-Gly-Asp-Tyr-Lys-Gly-Ala-Asp-Glu-Phe-Ala-Lys, wherein the amino acid residue order is DPGTGDYKGADEFAK shown in the SEQ ID NO: 1. The BSFN-I has the molecular weight of 11.9kDa. The preparation method comprises the following steps of homogenating mustelus griseus cartilage, carrying out guanidine hydrochloride extraction, carrying out acetone precipitation and grading, and carrying out ion exchange chromatography and gel chromatography to obtain a single peak so that it is proved that the mustelus griseus cartilage blood vessel generation inhibiting factor BSFN-I is obtained. The mustelus griseus cartilage blood vessel generation inhibiting factor BSFN-I can be used for preparation of drugs for inhibiting blood vessel generation and preventing and treating tumors.

Description

Technical field [0001] The present invention involves a preparation method for the gray-star shark chondroma-generating inhibitory factors and its N-terminal amino acid sequence. The invention also involves the application of gray-star shark chondromes inhibitory factors in preparation of inhibitory blood vessel production and preventing tumor drugs. Background technique [0002] Vascular production plays an important role in normal physiological processes such as human development and tissue repair. It is also one of the important pathological characteristics of tumor, diabetic retinopathy, rheumatoid arthritis, and chronic inflammation. [0003] In 1970, Folkman believed that the growth of tumors depended on the generation of new blood vessels, and the purpose of treating tumors by inhibiting the formation of tumor blood vessels can be achieved.At present, existing studies have proved that when the size of the tumor reaches 2 ~ 3 mm 2 The above must rely on neonatal blood vesse...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/435C07K1/18C07K1/16A61K38/17A61P9/00A61P35/00
Inventor 迟长凤王斌罗红宇栗丽王玉梅
Owner ZHEJIANG OCEAN UNIV
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