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An Inhibitory Factor of Cartilage Angiogenesis in Scalloped Hammerhead Shark

An angiogenesis-inhibiting, scalloped hammerhead technology, applied in the field of cartilage angiogenesis inhibitors of scalloped hammerhead sharks, can solve the problems that the application of scalloped hammerhead cartilage angiogenesis inhibitors has not been reported, and achieve significant activity , the effect of advanced purification process

Active Publication Date: 2020-08-11
ZHEJIANG OCEAN UNIV
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Problems solved by technology

[0005] The applicant's research found that the research on the preparation of angiogenesis inhibitory factors using scalloped hammerhead cartilage as raw material is in a blank stage, and the application of scalloped hammerhead cartilage angiogenesis inhibitors in the preparation of drugs for inhibiting angiogenesis and preventing and treating tumors It has not been reported

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  • An Inhibitory Factor of Cartilage Angiogenesis in Scalloped Hammerhead Shark
  • An Inhibitory Factor of Cartilage Angiogenesis in Scalloped Hammerhead Shark
  • An Inhibitory Factor of Cartilage Angiogenesis in Scalloped Hammerhead Shark

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Embodiment

[0024] Example: A preparation method of angiogenesis inhibitory factor of scalloped hammerhead shark, the preparation process is as follows: scalloped hammerhead shark cartilage → tissue crushing → guanidine hydrochloride extraction → acetone fractional precipitation → gel filtration chromatography purification → cell membrane chromatography Purification → high performance liquid chromatography purification → angiogenesis inhibitory factor.

[0025] 1) Preparation of active protein components of scalloped hammerhead shark cartilage: Add the crushed and homogenized sphyrnalewini cartilage into 1.0 mol / L guanidine hydrochloride solution at a solid-to-liquid ratio of 1 g: 10 mL. After shaking and extracting for 36 hours, centrifuge at 4°C and 10 000 r / min for 20 minutes, take the supernatant and put it into a dialysis bag with a molecular weight cut-off of 1 kDa, and use Tris-HCl (pH7.6) buffer solution to dialyze at below 4°C for 24 hours. Get the crude extract of hammerhead sha...

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Abstract

The invention relates to an angiogenesis inhibitory factor for cartilage of Sphyrna lewini, a preparation method therefor and application thereof. The amino acid sequence of the angiogenesis inhibitory factor is Pro-Asp-Tyr-Lys-Phe-Lys, and the ESI-MS detection molecular weight is 796.90Da. The angiogenesis inhibitory factor can be used for effectively inhibiting angiogenesis of a chick chorioallantoic membrane, and has an obvious inhibitory effect on three angiogenesis promoting factors, namely vascular endothelial cell growth factor, basicfibroblast growth factor and platelet derivative growth factor of a lung cancer tissue of a mouse bearing Lewis cancer.

Description

[0001] An Inhibitory Factor of Cartilage Angiogenesis in Scalloped Hammerhead Shark technical field [0002] The invention relates to an angiogenesis inhibitory factor extracted from aquatic organisms, in particular to a cartilage angiogenesis inhibitory factor of scalloped hammerhead shark. Background technique [0003] Angiogenesis is one of the important pathological features of vascular proliferative diseases such as tumors, diabetic retinopathy, rheumatoid arthritis and chronic inflammation. The anti-angiogenesis-based tumor biotherapy research has become a research hotspot of anti-tumor drugs in the past ten years. [0004] Compared with chemotherapy aimed at directly killing tumor cells, tumor angiogenesis inhibitor (Tumorangiogenesis inhibitor, TAI) has the following unique advantages: (1) TAI directly acts on vascular endothelial cells, while anticancer drugs are affected by tissue diffusion. Due to the influence of tissue fibrosis and necrosis, the effective drug ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/06C07K1/20C07K1/16C12P21/06
Inventor 王斌迟长凤赵玉勤孙坤来
Owner ZHEJIANG OCEAN UNIV
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