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Application of dasyatis akajei cartilage polypeptide angiogenesis inhibition factor

An angiogenesis inhibition and angiogenesis technology, applied in the fields of cardiovascular system diseases, applications, food preparation, etc., can solve the problems of difficult drug development, large molecular weight and low content of active substances, and achieve high accuracy, significant activity, and purification process advanced effects

Inactive Publication Date: 2015-09-02
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although it has been reported to prepare angiogenesis inhibitors from cartilage such as red ray and hole ray, the obtained active substances are difficult to develop drugs because of their large molecular weight and low content.

Method used

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  • Application of dasyatis akajei cartilage polypeptide angiogenesis inhibition factor
  • Application of dasyatis akajei cartilage polypeptide angiogenesis inhibition factor
  • Application of dasyatis akajei cartilage polypeptide angiogenesis inhibition factor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0012] A preparation method of red ray cartilage polypeptide angiogenesis inhibitory factor, the preparation process is as follows: red ray cartilage → tissue crushing → guanidine hydrochloride extraction → ultrafiltration → cell membrane stationary phase adsorption → comparative analysis of HPLC fingerprints → angiogenesis inhibitory factor.

[0013] 1) Preparation of cell membrane suspension: Hepatocellular carcinoma cells Bel-7402 in logarithmic growth phase with high expression of vascular endothelial growth factor receptor (VEGFR) were taken, and MEM medium (containing 10% fetal bovine serum) was used to incubate at 37°C, 5% CO 2 Culture in the incubator, when the cell count reaches 10 7 At the same time, the cells were digested with trypsin, centrifuged at 4°C and 1000 r / min for 10 min, the precipitate was washed twice with normal saline, and then added to 50 mM Tris-HCl solution for 30 min sonicated to disrupt the cells. The suspension was centrifuged at 1000 r / min, 4...

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Abstract

The invention relates to a dasyatis akajei cartilage polypeptide angiogenesis inhibition factor as well as a rapid preparation method and application thereof. The amino acid sequence of the dasyatis akajei cartilage polypeptide angiogenesis inhibition factor is Gly-Tyr-Ile-Cys-Pro-Gln-Trp (GYICPQW); the ESI-MS (electrospray ionization mass spectrometry) detection molecular weight is 865.98Da; the dasyatis akajei cartilage polypeptide angiogenesis inhibition factor can effectively inhibit angiogenesis of chorioallantoic membrane (CAM) and shows obvious effect on habiting the expression of three important pro-angiogenic factors of cancer tissue of a mat suffering from Lewis lung cancer, namely, a vascular endothelial cell growth factor (VEGF), a basic fibroblast growth factor (bFGF) and a platelet-derived growth factor (PDGF); therefore, the dasyatis akajei cartilage polypeptide angiogenesis inhibition factor can be used for preparing functional foods and additives for inhibiting angiogenesis as well as preventing and treating tumor.

Description

technical field [0001] The present invention also relates to the use of a marine biologically active polypeptide, in particular to the use of a red ray cartilage polypeptide angiogenesis inhibitory factor. Background technique [0002] Both tumor growth and metastasis depend on the formation of new blood vessels. Anti-angiotherapy therapy has the advantages of high efficiency, low toxicity, and less drug resistance. Therefore, searching for angiogenesis inhibitors with significant activity has become a research hotspot of antitumor drugs. Tumor angiogenesis is affected by a variety of factors, and vascular endothelial growth factor (VEGF) is the most important angiogenic factor and the main executor of VEGF signal transduction in endothelial cells, directly participating in the process of neovascularization. Studies have shown that VEGF must play a role by binding to the specific receptor VEGFR-2 located on the cell membrane, so drug screening targeting VEGFR-2 has become a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/08A61P35/00A61P9/00A23L1/29
Inventor 王斌潘欣迟长凤陈荫赵玉勤孙坤来
Owner ZHEJIANG OCEAN UNIV
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