A red ray cartilage polypeptide angiogenesis inhibitory factor

An angiogenesis inhibition and peptide technology, applied in the direction of peptides, can solve the problems of low content, difficult drug development, and large molecular weight of active substances, and achieve the effect of advanced purification process, high accuracy, and significant activity

Active Publication Date: 2020-08-11
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although it has been reported to prepare angiogenesis inhibitors from cartilage such as red ray and hole ray, the obtained active substances are difficult to develop drugs because of their large molecular weight and low content.

Method used

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  • A red ray cartilage polypeptide angiogenesis inhibitory factor
  • A red ray cartilage polypeptide angiogenesis inhibitory factor
  • A red ray cartilage polypeptide angiogenesis inhibitory factor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0020] A preparation method of red ray cartilage polypeptide angiogenesis inhibitor. The preparation process is as follows: red ray cartilage-tissue fragmentation-guanidine hydrochloride extraction-ultrafiltration-cell membrane stationary phase adsorption-HPLC fingerprint spectrum comparative analysis-angiogenesis inhibitor.

[0021] 1) Preparation of cell membrane suspension: take vascular endothelial growth factor specific receptor (VEGFR) highly expressed liver cancer cell Bel-7402 in the logarithmic growth phase, using MEM medium (containing 10% fetal bovine serum), At 37℃, 5% CO 2 Culture in an incubator, when the cell count reaches 10 7 At this time, the cells were digested with trypsin, centrifuged at 4°C and 1000 r / min for 10 min, the precipitate was taken and washed twice with physiological saline, and then added to 50 mM Tris-HCl solution and sonicated for 30 min to disrupt the cells. Centrifuge the suspension at 1000r / min and 4°C for 5min. Take the supernatant and centr...

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Abstract

The invention relates to a dasyatis akajei cartilage polypeptide angiogenesis inhibitor, and a rapid preparation method and an application thereof. An amino acid sequence of the angiogenesis inhibitor is Gly-Tyr-Ile-Cys-Pro-Gln-Trp; and the molecular weight of ESI-MS (electrospray ionization mass spectrometry) detection is 865.98Da. The angiogenesis inhibitor is capable of effectively inhibiting angiogenesis of chick chorioallantoic membrane (CAM), and has an obvious inhibiting effect on expression of three pro-angiogenic factors, namely a Lewis lung cancer mice lung cancer tissue-loaded vascular endothelial cell growth factor (VEGF), a basic fibroblast growth factor (bFGF) and a platelet-derived growth factor (PDGF).

Description

Technical field [0001] The present invention relates to a marine biological active polypeptide, in particular to a red ray cartilage polypeptide angiogenesis inhibitor. Background technique [0002] The growth and metastasis of tumors depend on the formation of new blood vessels. The use of anti-vascular therapy to treat tumors has the advantages of high efficiency, low toxicity, and resistance to drug resistance. Therefore, looking for significantly active angiogenesis inhibitors has become a research hotspot in anti-tumor drugs. Tumor angiogenesis is affected by many factors, and vascular endothelial growth factor (VEGF) is the most important angiogenic factor and the main performer of endothelial cell VEGF signal transduction, directly involved in the process of neovascularization. Studies have shown that VEGF must work by binding to the specific receptor VEGFR-2 located on the cell membrane. Therefore, drug screening with VEGFR-2 as the target has become an important strateg...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/06
Inventor 王斌潘欣迟长凤陈荫赵玉勤孙坤来
Owner ZHEJIANG OCEAN UNIV
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