The invention discloses a preparation method of collagen micromolecule active
peptide and
bioactive peptide for inhibiting
cancer cell proliferation and improving
intracellular environment, and belongs to the technical field of
bioactive peptide preparation and biological functional raw materials. According to the method,
bone collagen peptide,
cartilage peptide (hydrolyzed II-type
collagen peptide),
collagen peptide (German Jilida), non-denatured II-type
collagen peptide and yak
bone marrow peptide are taken as core raw materials, and optional ingredients such as
bovine bone marrow peptide
powder,
shark cartilage peptide and Belgian imported
inulin can be compounded as required; through a step-by-step preparation process comprising the steps of graded purification pretreatment of raw materials, precise proportioning and mixing, low-temperature activation and synergism and stable micro-
capsule embedding, structure protection and
efficacy synergy of each collagen small-molecule active peptide and bioactive components are realized; the prepared
bioactive peptide product can significantly inhibit
cancer cell proliferation, regulate
intracellular oxidation-reduction balance and improve
intracellular microenvironment, the preparation process is mild and free of high-temperature damage, the
raw material biological activity retention rate is high, the
raw material source is controllable, the large-scale production feasibility is high, and the bioactive peptide product can be widely applied to preparation in the fields of
functional food, bioactive raw materials and the like.