27 results about "Demineralized bone" patented technology

A composition for the treatment of wounds includes demineralized bone fibers (DBF) derived from allogeneic or xenogenic cortical bone and / or polymeric fibers made from resorbable and / or non-resorbable polymer, and the composition may also include an oxygen-generating material and / or an oxygen carrier.

An osteogenic composition comprises growth-factor augmented cortical bone and cancellous bone having viable endogenous osteogenic cells that remain adherent to the cancellous bone. The growth-factor augmented cortical bone comprises demineralized cortical bone amplified with growth factors derived from a demineralized bone, and the cancellous bone not having been subjected to a loosening agent. The osteogenic composition is cryopreserved or lyopreserved. A method for preparing an enhanced graft composition with improved osteoinductive properties includes processing demineralized cortical bone; solubilizing growth factors from demineralized bone; and mixing the demineralized cortical bone with the solubilized growth factors.

One embodiment of the present invention provides a method of producing a fibrous non-demineralized bone graft material, the method comprising: a step of removing soft tissue, blood, and bone marrow from bone; a dehydration step of immersing, in an aqueous ethanol solution, the bone from which the soft tissue, blood, and bone marrow have been removed, and stirring same; a primary defatting step of immersing and stirring the dehydrated bone in diethyl ether; a step of crushing the bone subjected to the primary defatting into fibrously processed bone; a step of screening the fibrously processed bone; a hydration step of immersing and stirring the screened fibrously processed bone in ethanol; a secondary defatting step of immersing and stirring the hydrated fibrously processed bone in hydrogen peroxide; a washing step of washing the fibrously processed bone subjected to the secondary defatting; and a freeze-drying step.

An apparatus for mixing bone material with a liquid is provided. The apparatus includes a chamber having a proximal end and a distal end and bone material disposed within the chamber, the bone material comprising DBM particles of milled and lyophilized demineralized bone fibers; and a plunger having at least a portion slidably disposed within the proximal end of the chamber and configured to dispense the bone material mixed with the liquid from the distal end of the chamber when the plunger is in an extended position.

The invention discloses a bone-derived collagen peptide with a joint protection effect and a preparation method thereof, and belongs to the technical field of collagen peptide preparation, the preparation method comprises the following steps: S1, cleaning and crushing fresh fish bones, and performing impurity protein removal treatment on fish bone powder by using a 0.1 M NaOH solution to obtain pretreated bone powder; s2, mixing the pretreated bone meal with 0.5-1.5 mol / L hydrochloric acid according to a solid-to-liquid ratio of 1: 5, and carrying out a decalcification reaction for 2-4 hours to obtain decalcified bone collagen; s3, performing sequential enzymolysis on the decalcified ossein by adopting pepsin and trypsin to obtain enzymatic hydrolysate; and carrying out inactivation, purification, vacuum concentration and freeze drying on the enzymatic hydrolysate to obtain the collagen peptide. According to the collagen peptide and the preparation method, the characteristic amino acid content of the prepared collagen peptide is increased by 40% or above compared with that of a traditional process, it is ensured that the collagen peptide is combined with a cartilage cell receptor in a targeted mode and inhibits secretion of inflammatory factors, and the inhibition rate reaches 58-68%; at a concentration of 500 [mu] g / mL, there is no toxicity to HaCaT cells (IC50gt; and the traditional Chinese medicine composition has no toxic or side effect and no skin irritation.

A process for the production of bone gelatin includes demineralization of bone using a demineralization solution of acid, wherein the bone is washed for a demineralized time in the demineralization solution to remove soft tissue. The demineralized bone is then pulverized to produce particles of the demineralized bone in a homogenized bone tissue. Lipids are then removed from the homogenized bone tissue using a lipid removal series of washing steps which may include washing the homogenized bone tissue in a series of solutions, including a lipid removal solution and an ethanol solution. Gelatin is then removed from the lipid removed bone tissue in a gelatin extraction process, wherein lipid removed bone tissue is placed in a pepsin solution followed by an extraction solution. The gelatin from the gelatin extracted bone tissue may then be separated and the bone gelatin may then be lyophilized.

A process for the production of bone gelatin includes demineralization of bone using a demineralization solution of acid, wherein the bone is washed for a demineralized time in the demineralization solution to remove soft tissue. The demineralized bone is then pulverized to produce particles of the demineralized bone in a homogenized bone tissue. Lipids are then removed from the homogenized bone tissue using a lipid removal series of washing steps which may include washing the homogenized bone tissue in a series of solutions, including a lipid removal solution and an ethanol solution. Gelatin is then removed from the lipid removed bone tissue in a gelatin extraction process, wherein lipid removed bone tissue is placed in a pepsin solution followed by an extraction solution. The gelatin from the gelatin extracted bone tissue may then be separated and the bone gelatin may then be lyophilized.

A method of repairing a chondral or osteochondral defect using an implant composition made of a plurality of demineralized bone fibers formed into a contiguous shape having a peg portion and a sheet portion to facilitate cartilage repair.

A method of making a bone graft composition is described wherein material including decalcified bone elongated particulate bone is dried in a constraining device or under pressure to produce a solid construct having dimensions corresponding to the constraining device or space to which the material occupied under pressure.

A pharmaceutical composition for treating moderate or severe rosacea comprises a formulated, body weight-independent low dose of minocycline that reduces the severity of rosacea in at least 2 weeks with a decrease in lesion count, Investigator's Global Assessment (IGA) score, and Clinician's Erythema Assessment (CEA) score with a measured improvement in quality of life.

The invention discloses a demineralized bone matrix fiber as well as a preparation method and application thereof. The method comprises the following steps: firstly, cleaning and disinfecting natural bones separated out of a body, then cutting the natural bones into bone blocks, further degreasing the bone blocks, and cutting the bone blocks into fibers with the length of 1-100mm and the thickness of 0.2-1.4 mm; then placing the bone fibers obtained by cutting in an acidic demineralization solution for demineralization until the calcium content is 0-20%; the method comprises the following steps: demineralizing bone fibers, then reacting with the demineralized bone fibers by using an anionic surfactant for 0.5-3 hours, cleaning, then reacting for 12-18 hours by using a nonionic surfactant, completing two-stage decellularization, cleaning, and freeze-drying for preservation. During clinical use, the demineralized bone fibers which are kept in a freeze-drying manner are mixed with hydrated liquid or mixed with one of sodium carboxymethyl cellulose, gelatin, poloxamer and chitosan to form an ointment substance which is used as a bone defect repairing material.

A bone implant composition and methods thereof include bone material made into various implant shapes including a cylinder having an outermost layer and an inner layer completely surrounded by the outermost layer. The bone implant compositions and methods include a bag or cylindrical tube made from a bone material which may be filled with additional bone material.

A composition and methods thereof include bone fibers made from cortical bone in which a plurality of bone fibers are made into shapes that are used to augment rotator cuff and acl repair. Sheets of bone fibers may be used as an interface between bone and tissue, tendons, and / or ligaments. The physical presence of the fibers provides initial fixation, while the use of an osteoinductive material provides long term enhancement of bone formation. A delivery system and methods of use are also provided.

A process for the production of bone gelatin includes demineralization of bone using a demineralization solution of acid, wherein the bone is washed for a demineralized time in the demineralization solution to remove soft tissue. The demineralized bone is then pulverized to produce particles of the demineralized bone in a homogenized bone tissue. Lipids are then removed from the homogenized bone tissue using a lipid removal series of washing steps which may include washing the homogenized bone tissue in a series of solutions, including a lipid removal solution and an ethanol solution. Gelatin is then removed from the lipid removed bone tissue in a gelatin extraction process, wherein lipid removed bone tissue is placed in a pepsin solution followed by an extraction solution. The gelatin from the gelatin extracted bone tissue may then be separated, and the bone gelatin may then be lyophilized.

A composition and methods thereof include bone fibers made from cortical bone in which a plurality of bone fibers are made into shapes that are used to augment rotator cuff and acl repair. Sheets of bone fibers may be used as an interface between bone and tissue, tendons, and / or ligaments. The physical presence of the fibers provides initial fixation, while the use of an osteoinductive material provides long term enhancement of bone formation. A delivery system and methods of use are also provided.

The invention discloses a preparation method of an allogeneic bone shaping material, which comprises the following steps: S1, bone pretreatment to obtain freeze-dried bone powder and demineralized bone powder; s2, bone gelatin extraction; s3, preparing an excipient, wherein the bone gelatin in the step S2 is added into water containing polyvinylpyrrolidone to be swelled; and S4, mixing the freeze-dried bone powder and the demineralized bone powder in the step S1 with the excipient in the step S3 in proportion to obtain the allogeneic bone shaping material. According to the preparation method, polyvinylpyrrolidone is added, the proportion of freeze-dried bone powder to demineralized bone powder in the mixed bone powder is adjusted, the degradation rate of the allogeneic bone powder can be adjusted, and the allogeneic bone powder can be effectively degraded on the basis that good bone induction and bone conduction performance of the allogeneic bone powder is reserved. And the material is endowed with good injectability and plasticity.

Disclosed herein are methods of making an implant material, the method involving harvesting allograft cancellous bone containing bone marrow from a donor within 24 hours of death; incubating the harvested allograft cancellous bone in an ultra-low freezer within 144 hours of death for at least 5 days; and then selectively lysing cells in the cancellous bone. This results in cancellous bone that is enriched for cells resistant to lysing, such as mesenchymal cells as well as a lysate containing factors (e.g. growth factors) released from the lysed cells. This lystate can be administered to cortical bone, e.g. demineralized cortical bone, with hydrochloric acid (HCI) or glycolytic acid so that these factors attach to the demineralized bone.

A tissue engineering cartilage constructed based on a decalcified bone scaffold, a preparation method therefor, and use thereof in the repairing of joint defects are provided. A marrow fluid of a patient is extracted, bone marrow mesenchymal stem cells of the patient are isolated and amplified in vitro, and the bone marrow mesenchymal stem cells isolated and cultured in vitro are inoculated into a decalcified bone scaffold, thus constructing a tissue engineering cartilage in vitro. The tissue engineering cartilage can be transplanted to a joint defect part, so that the effective repair of joint defects and function reconstruction are realized.

The present invention provides an enhanced osteogenic composition comprising of connective tissue proteins having molecular weights greater than or equal to 3.5kDa wherein the composition is prepared by treating demineralized bone material in an acidic extraction medium at a pH between about 0.10 to 0.45 at an extraction temperature between greater than 25° C and less than 80° C for a predetermined time period. The present invention further provides a method of making the enhanced osteogenic composition.

Disclosed are various bioactive grafts and methods of making the same. In one embodiment, bone material is harvested from a donor. The harvested bone material is exposed to a lysing agent, the lysing agent configured to release growth factors and bioactive materials from cellular material of the harvested bone material. The harvested bone material is then rinsed with a rinsing agent. The pH of the harvested bone material is substantially neutralized.

A composition for the treatment of wounds includes demineralized bone fibers (DBF) derived from allogeneic or xenogenic cortical bone and / or polymeric fibers made from resorbable and / or non-resorbable polymer, and the composition may also include an oxygen-generating material and / or an oxygen carrier.

An osteogenic composition comprises growth-factor augmented cortical bone and cancellous bone having viable endogenous osteogenic cells that remain adherent to the cancellous bone. The growth-factor augmented cortical bone comprises demineralized cortical bone amplified with growth factors derived from a demineralized bone, and the cancellous bone not having been subjected to a loosening agent. The osteogenic composition is cryopreserved or lyopreserved. A method for preparing an enhanced graft composition with improved osteoinductive properties includes processing demineralized cortical bone; solubilizing growth factors from demineralized bone; and mixing the demineralized cortical bone with the solubilized growth factors.

An osteogenic composition comprises growth-factor augmented cortical bone and cancellous bone having viable endogenous osteogenic cells that remain adherent to the cancellous bone. The growth-factor augmented cortical bone comprises demineralized cortical bone amplified with growth factors derived from a demineralized bone, and the cancellous bone not having been subjected to a loosening agent. The osteogenic composition is cryopreserved or lyopreserved. A method for preparing an enhanced graft composition with improved osteoinductive properties includes processing demineralized cortical bone; solubilizing growth factors from demineralized bone; and mixing the demineralized cortical bone with the solubilized growth factors.

Disclosed herein are three-dimensional bone tissue grafts produced from stacked demineralized bone paper. Also disclosed are methods for treating a subject using tissue grafts produced from the disclosed demineralized bone paper. Also disclosed are assay systems that involves culturing bone-promoting cells on the disclosed demineralized bone paper.

The embodiment of the invention discloses a method for constructing a bone micro-tissue by compounding a bone extracellular matrix with a vascular matrix component and application of the bone micro-tissue. The specific implementation mode of the method comprises the following steps: preprocessing a human femoral head cortical bone sample; performing freeze thawing treatment; preparing demineralized bone powder corresponding to the caput femoris cortical bone sample of the target person; preparing a bone source extracellular matrix microcarrier; preparing a vascular matrix component; mixing the vascular matrix component and the bone-derived extracellular matrix microcarrier in a basic culture medium to obtain a mixed suspension; transferring the mixed suspension to a rotary bioreactor; and performing dynamic culture treatment on the vascular matrix component and the bone source extracellular matrix microcarrier in the basic culture medium to obtain the bone micro-tissue. According to the implementation mode, the effect of the constructed bone micro-tissue can be improved, the secondary injury to a patient and the risk of complications are reduced, and the effect of repairing bone defects can be improved.

An implantable mesh including demineralized bone fibers mechanically entangled into a biodegradable or permanent implantable mesh is provided. A method of preparing the implantable mesh is also provided. The method of preparing the implantable mesh includes mechanically entangling demineralized bone fibers with non-bone fibers to form the implantable mesh. The mechanical entanglement of the bone fibers into the implantable mesh is achieved by applying needle punching with barbed needles, spun lacing, entanglement with water jets or air jets or ultrasonic entanglement with ultrasonic waves. A method of implanting an implantable mesh at a target bone tissue site is also provided.

Disclosed are methods for forming a bone graft by rolling a bundle of demineralized bone fibers to form a bone graft material with increased flexural strength.