Production method for bacillus licheniformis with high sporation rate

A technology of Bacillus licheniformis and production methods, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of prolonging the fermentation time, increasing production costs, and limiting the amount of manganese, so as to improve the sporulation rate, The effect of good product quality and reduced fermentation production cost

Active Publication Date: 2015-11-18
LINZHOU SINAGRI YINGTAI BIOLOGICAL PEPTIDES CO LTD
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Problems solved by technology

[0003] The existing general production process is to add 0.02% manganese sulfate in the fermentation base medium. Manganese ions can promote the formation of spores, but manganese ions can also inhibit the growth and reproduction of Bacillus licheniformis, so the amount of manganese is reduced To the limit, the sporulation rate is low, only about 85%. To increase the sporulation rate, the fermentation time will be prolonged and the production cost will be increased.

Method used

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  • Production method for bacillus licheniformis with high sporation rate
  • Production method for bacillus licheniformis with high sporation rate

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Effect test

Embodiment 1

[0020] The production method of a hyperbophabal ground, including the following steps:

[0021] 1) Cultivation of bottle of bottle: Inocularly inocus to the bottle medium, the vaccination amount is 1: (80 ~ 120) to the bed in the bed;37 ° C, the cultivation time is 10 ~ 12 hours; the protein is 8 ~ 12g per liter bottle medium, 3 ~ 7g of yeast powder, 3 ~ 7g of salt, the rest is water; the rest are water; the rest are water;

[0022] 2) Seed tank culture: Putting steps 1) The obtained device Bacillus liquid is inoculated to the seed medium, the vaccination amount is trained at a volume ratio of 1: (80 ~ 120), the speed is 180 ~ 220rpm / min, the temperature is 35 ~ 35 ~37 ° C, the cultivation time is 10 ~ 12 hours; the seed medium formula is: protein 胨 1.5 ~ 2.5%, yeast powder 0.5 ~ 1.5%, sodium chloride 0.4 ~ 0.6%.

[0023] 3) Cultivation of fermentation tanks: Step 2) The obtained Lidobacteria bacteria liquid is inoculated to the fermentation foundation medium, and the amount of va...

Embodiment 2

[0026] The production method of a hyperbophabal ground, including the following steps:

[0027] 1) Shake bottle culture: Pretend to 1L medium according to the formula, the average packaging and 10 1000ml triangle bottle, after high temperature sterilization and cooling, use the ground to vaccinate with the ground, the amount of inoculation is 100, the bed is cultivated, the speed is 220rpm / min, the speed is 220rpm / min.The temperature is 37 ° C, the cultivation time is 11 hours;

[0028] Cultivation base formula: 10g of protein 胨; 3 to 7g of yeast powder; 3 to 7g of salt, setting it with distilled water to 1L, high -pressure steam 121 ℃ sterilized;

[0029] 2) Seed tank culture: Curma formula: 2%protein 胨, 1%yeast powder, 0.5%sodium chloride. Drinking water preparation, 300 liters of volume, high -pressure steam 121 ℃ sterilized for 30 minutes, vaccination volume 1: 100, speed is the speed of speed, the speed is to the speed, the speed is to the speed, the speed is to the speed, th...

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Abstract

The invention provides a production method for bacillus licheniformis with a high sporation rate. According to the production method, no manganese chloride is added into a fermentation medium in a fermentation tank, a sterilized manganese chloride solution is supplemented after fermentation is carried out for 23-25 h, and the volume of the sterilized manganese chloride solution accounts for 0.03-0.04 % of the fermentation volume. As manganese chloride is added in the middle and later stage of fermentation, early-stage growth of the bacillus licheniformis is not affected, the sporation rate of the bacillus licheniformis is increased, the fermentation cycle is shortened, the fermentation production cost is reduced, and better product quality is achieved.

Description

Technical field [0001] The present invention is a biological fermentation technology field, which involves a production method for a high -olical spores. Background technique [0002] Clatlary Bacillus has a strong activity of protease, lipase, and amylase, which can promote the degradation of nutrients in the feed, stimulate the development of animal immune organs.The mechanism can inhibit the growth and reproduction of the pathogenic bacteria. It is a green feed additive. After the vein of the ground, the spores formation have strong resistance, heat resistance, not easy to die, and easy to store.Therefore, increasing the method of spores during the fermentation of ground jackets is a focus of fermentation production process. [0003] The existing general production process is to add 0.02%manganese sulfate to the foundation medium of the fermentation foundation. Manganese ions have the effect of promoting spore formation.To the limit, the spore rate is low, only about 85%. To i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/38C12N1/20C12R1/10
Inventor 刘扬科郭文江胡国春杨婧芳宋宇鹏赵福生王庆芳龙婉庆路广亮
Owner LINZHOU SINAGRI YINGTAI BIOLOGICAL PEPTIDES CO LTD
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