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A kind of production method of bacillus licheniformis with high spore rate

A technology of Bacillus licheniformis and production methods, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of prolonging the fermentation time, increasing production costs, and limiting the amount of manganese, so as to improve the sporulation rate, The effect of good product quality and reduced fermentation production cost

Active Publication Date: 2018-10-02
LINZHOU SINAGRI YINGTAI BIOLOGICAL PEPTIDES CO LTD
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  • Claims
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Problems solved by technology

[0003] The existing general production process is to add 0.02% manganese sulfate in the fermentation base medium. Manganese ions can promote the formation of spores, but manganese ions can also inhibit the growth and reproduction of Bacillus licheniformis, so the amount of manganese is reduced To the limit, the sporulation rate is low, only about 85%. To increase the sporulation rate, the fermentation time will be prolonged and the production cost will be increased.

Method used

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  • A kind of production method of bacillus licheniformis with high spore rate

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Embodiment 1

[0020] A kind of production method of bacillus licheniformis with high spore rate comprises the following steps:

[0021] 1) Shake flask culture: inoculate Bacillus licheniformis on the shake flask culture medium, the inoculation amount is 1:(80~120) according to the volume ratio, and carry out shaker culture; the shaker speed is 180~220rpm / min, the temperature is 35~ 37°C, culture time 10-12 hours; in each liter of shake flask medium, peptone is 8-12g, yeast powder is 3-7g, salt is 3-7g, and the rest is water;

[0022] 2) Seed tank cultivation: inoculate the Bacillus licheniformis bacteria liquid obtained in step 1) on the seed medium, and cultivate the inoculum according to the volume ratio of 1: (80~120), the speed is 180~220rpm / min, and the temperature is 35~ 37°C, culture time 10-12 hours; the formula of the seed medium is: peptone 1.5-2.5%, yeast powder 0.5-1.5%, sodium chloride 0.4-0.6%, the rest is water, and the content of the above-mentioned raw materials is the mass...

Embodiment 2

[0026] A kind of production method of bacillus licheniformis with high spore rate comprises the following steps:

[0027] 1) Shake flask culture: prepare 1L culture medium according to the recipe, evenly distribute it into ten 1000ml Erlenmeyer flasks, after high-temperature sterilization and cooling, inoculate with Bacillus licheniformis, the inoculum size is 100, and cultivate on a shaking table at a speed of 220rpm / min. The temperature is 37°C, and the incubation time is 11 hours;

[0028] Medium formula: peptone 10g; yeast powder 3-7g; salt 3-7g, distilled water to 1L, high-pressure steam at 121°C for sterilization;

[0029] 2) Seed tank culture: medium formula: peptone 2%, yeast powder 1%, sodium chloride 0.5%. Drinking water preparation, volume 300 liters, high-pressure steam sterilization at 121°C for 30 minutes, inoculum size 1:100, speed of 200rpm / min, temperature 36°C, incubation time 10 hours;

[0030] 3) Fermentation tank culture: prepare 10 tons of fermentation ...

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Abstract

The invention provides a production method for bacillus licheniformis with a high sporation rate. According to the production method, no manganese chloride is added into a fermentation medium in a fermentation tank, a sterilized manganese chloride solution is supplemented after fermentation is carried out for 23-25 h, and the volume of the sterilized manganese chloride solution accounts for 0.03-0.04 % of the fermentation volume. As manganese chloride is added in the middle and later stage of fermentation, early-stage growth of the bacillus licheniformis is not affected, the sporation rate of the bacillus licheniformis is increased, the fermentation cycle is shortened, the fermentation production cost is reduced, and better product quality is achieved.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation, and in particular relates to a production method of bacillus licheniformis with a high spore rate. Background technique [0002] Bacillus licheniformis has strong protease, lipase and amylase activities, which can promote the degradation of nutrients in feed, stimulate the development of animal immune organs, enhance the body's immunity, produce anti-active substance enzymes, and have a unique biological oxygen capture effect The mechanism can inhibit the growth and reproduction of pathogenic bacteria. It is a green feed additive. After the formation of spores by Bacillus licheniformis, it has strong stress resistance, heat resistance, is not easy to die, and is easy to store. Therefore, improving the sporulation method in the fermentation process of Bacillus licheniformis is an important point in the fermentation production process. [0003] The existing general production proce...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/38C12N1/20C12R1/10
Inventor 刘扬科郭文江胡国春杨婧芳宋宇鹏赵福生王庆芳龙婉庆路广亮
Owner LINZHOU SINAGRI YINGTAI BIOLOGICAL PEPTIDES CO LTD
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