43results about How to "Reduce the cost of fermentation production" patented technology

A method for producing Gamma-glutamyltranspeptidase by using Bacillus subtilis belongs to the biotechnology field of foods. The method carries out the three-stage culture of the slope, the seeds and the fermentation of the producing bacteria Bacillus subtilis SK11.004 of the Gamma-glutamyltranspeptidase; and the Gamma-glutamyltranspeptidase powder is obtained by carrying out separation and purification of fermentation broth. The provided method for producing the Gamma-glutamyltranspeptidase by using the Bacillus subtilis SK 11.004 has small breeding workload, low cost and safe and reliable performances, the method can carry out the liquid fermentation production, the cost is low, the method can be used in large-scale production, and the enzyme activity of the Gamma-glutamyltranspeptidase which is produced by using the Bacillus subtilis can be up to more than 4U/mL. The fermented product is non-toxic and can be directly used for producing a plurality of valuable Gamma-glutamyl compounds, such as L-Gamma-glutamyl-L-dopa, Gamma-glutamyl taurine, Gamma-glutamyl tryptophan, glutathione, theanine, and the like.

The invention belongs to the field of fermentation engineering, relates to a method for producing ARA (arachidonic acid) through microbial fermentation, and particularly relates to a method for producing compound fat containing the ARA through industrial fermentation of a mortierella alpine strain. More particularly, the invention relates to a method for culturing microorganisms for producing the ARA, wherein the method for culturing the microorganisms for producing the ARA comprises the steps of controlling the fermentation temperature to be 20 to 25 DEG C after culturing in a fermentation tank for 100 to 140h; and/or, stopping ventilation or reducing the ventilatory capacity by not less than 50 percent at the 8th to 10th day of the culturing in the fermentation tank. The method for preparing the ARA has the advantages that the yield is high, the purity is high, and the large-scale industrial production of the ARA is facilitated.

The invention provides a production method for bacillus licheniformis with a high sporation rate. According to the production method, no manganese chloride is added into a fermentation medium in a fermentation tank, a sterilized manganese chloride solution is supplemented after fermentation is carried out for 23-25 h, and the volume of the sterilized manganese chloride solution accounts for 0.03-0.04 % of the fermentation volume. As manganese chloride is added in the middle and later stage of fermentation, early-stage growth of the bacillus licheniformis is not affected, the sporation rate of the bacillus licheniformis is increased, the fermentation cycle is shortened, the fermentation production cost is reduced, and better product quality is achieved.

The invention discloses a bio-synthesized gene cluster dis427 of Disorazole Z. The nucleotide sequence of the gene cluster is shown in SEQ ID No.1. The invention further discloses an engineering strain DK1622::Km-Ptet-dis427 used for heterologously expressing Disorazole Z efficiently and established through the gene cluster dis427. The engineering strain is obtained in the mode that Myxococcus xanthus DK1622 is used as an original strain, and the bio-synthesized gene cluster dis427 is integrated on a genome of the original strain with a transposition method. The invention further discloses application of the engineering strain DK1622::Km-Ptet-dis427 in preparation of Disorazole Z. A Disorazole Z bio-synthesis way and an efficient expression method of Disorazole Z in heterologous hosts havehigh research and application value in developing novel anti-tumor or anti-infection medicine and reducing the fermentation production cost.

The present invention relates to a fermentation production method of C19-C21 long-chain dibasic acids. The present invention discloses a method for producing the C19-C21 long-chain dibasic acids by fermentation of candida tropicalis 10468. The candida tropicalis 10468 can grow under high temperature conditions, can also efficiently convert C19-C21 normal alkanes with different carbon chain lengths, linear saturated fatty acids and linear saturated fatty acid derivatives into the C19-C21 long-chain dibasic acids, achieves high-temperature fermentation and efficient production of the C19-C21 long-chain dibasic acids, and can be used for the fermentation production of the C19-C21 long-chain dibasic acids in practice.

The invention discloses an engineering strain for heterologous expression of a histone deacetylase inhibitor FK228. The engineering strain is named as an engineering strain E264:: R-tdp-dep, and the engineering strain is obtained by using Burkholderia thailanensis E264 [delta] oprABC [delta] tdpDE5kb as an original strain and integrating a combined biosynthetic gene cluster R-tdp-dep on a genome of an original strain through a transposition method. The invention also discloses an application of the engineering strain in preparation of the histone deacetylase inhibitor FK228. Experiments prove that the yield of FK228 produced by the engineering strain E264:: R-tdp-dep reaches 94 mg/L and is nearly 5 times that of a current FK228 industrial production strain, the yield of the FK228 is greatly increased, the fermentation cost is reduced, the fermentation production period is shortened, a foundation is laid for large-scale preparation and development of histone deacetylase inhibitor drugs, The engineering strain has obvious economic value and social benefit.

The invention provides a novel photosynthetic organism hydrogen generation process based on the oscillation-light-filling strategy. Specifically, the invention provides a hydrogen generation method utilizing photosynthetic bacteria. The method comprises the following steps: (1) culturing photosynthetic bacteria capable of generating hydrogen under the nonstatic state and the irradiation of initial incident light with the light intensity I1, wherein the light intensity I1 of the initial incident light satisfies the following conditions: (i) I1>I0, I0 is the optimal light intensity of the initial incident light during static cultivation; (ii) under the light intensity I1, the actual light intensity I1' in at least 70% of area (or volume) of the culture system is larger than or equal to the actual light intensity I0' in the same area (or volume) during static cultivation; and (2) separating the generated hydrogen from the culture system. By using the method of the invention, hydrogen generation efficiency of photosynthetic bacteria can be effectively improved, hydrogen generation period can be shortened and high substrate conversion efficiency can be maintained.

The invention discloses a pretreatment method for producing butanol by fermentation of bagasse. The pretreatment method comprises the following steps of mechanical grinding, liquid-state hot-water treatment, microwave treatment, microbial decomposition, ammonia treatment and enzymolysis treatment, after the steps are performed, the decomposition products in each step can be mixed and subjected to step-by-step saccharification and fermentation and simultaneous saccharification and fermentation. By the pretreatment method, the contents of major inhibitors furancarboxaldehyde and hydroxymethyl furfural can be controlled below the concentration without affecting the growth of fermentative bacteria and other inhibitors such as glucuronic acid, p-coumaric acid, syringic acid and ferulic acid are not basically produced. Since the detoxification purification treatment of the pretreated decomposition products is not needed and the pretreated decomposition products are directly subjected to biological fermentation to produce butanol, the pretreatment method has the advantages that the production of inhibitors can be controlled, the cost is low, the yield is high and the environment friendliness is achieved.

The invention provides a method for producing fatty acid by fermenting lignocellulose ionic liquid hydrolysate. An escherichia coli DQ430 is utilized to regulate the total polysaccharide concentration of hydrolysate to be 10-50 g/L with water under an aerobic condition, fatty acid is produced by fermenting in batches or in fed-batch, and after fermenting for 24-72 hours, the concentration of fatty acid can be up to 3-15 g/L; the utilization rate of sugar is 60-90%, and the production intensity is up to 0.063-0.313g/L<-h>. The method for producing the fatty acid by fermenting the lignocellulose ionic liquid hydrolysate has the outstanding advantages that bamboo powder is used as a raw material to replace expansive glucose and is utilized as a carbon source to product fatty acid through fermentation; regenerated biomass resources are utilized, the environmental friendliness is achieved, and the shortage of petrifaction resource for chemic synthesis of fatty acid can be released.