44results about How to "Reduce the cost of fermentation production" patented technology

The invention discloses a method for producing trichoderma solid spawn by taking an amino acid hydrolytic solution and rice straw as raw materials. A filamentous fungus Trichoderma harzianum with a good plant rhizosphere growth-promoting effect is utilized, the rice straw rich in lignocellulose and abandoned livestock amino acid hydrolytic solution nutrients are fully utilized under the culture condition of 28 DEG C, rapid growth and spore production are achieved, after solid fermentation is conducted for 5 days to 6 days, the number of chlamydospores of trichoderma guizhouense NJAU4742 reaches up to 2.4*10<10> spore / g, the solid spawn is directly added into a decomposed organic fertilizer, a trichoderma amino acid bio-organic fertilizer of which the number of stable and effective living bacteria is larger than 2*10<8> can be rapidly produced, and the bio-organic fertilizer has a very good rhizosphere growth-promoting effect on tomatoes.

A method for producing Gamma-glutamyltranspeptidase by using Bacillus subtilis belongs to the biotechnology field of foods. The method carries out the three-stage culture of the slope, the seeds and the fermentation of the producing bacteria Bacillus subtilis SK11.004 of the Gamma-glutamyltranspeptidase; and the Gamma-glutamyltranspeptidase powder is obtained by carrying out separation and purification of fermentation broth. The provided method for producing the Gamma-glutamyltranspeptidase by using the Bacillus subtilis SK 11.004 has small breeding workload, low cost and safe and reliable performances, the method can carry out the liquid fermentation production, the cost is low, the method can be used in large-scale production, and the enzyme activity of the Gamma-glutamyltranspeptidase which is produced by using the Bacillus subtilis can be up to more than 4U / mL. The fermented product is non-toxic and can be directly used for producing a plurality of valuable Gamma-glutamyl compounds, such as L-Gamma-glutamyl-L-dopa, Gamma-glutamyl taurine, Gamma-glutamyl tryptophan, glutathione, theanine, and the like.

The invention belongs to the field of fermentation engineering, relates to a method for producing ARA (arachidonic acid) through microbial fermentation, and particularly relates to a method for producing compound fat containing the ARA through industrial fermentation of a mortierella alpine strain. More particularly, the invention relates to a method for culturing microorganisms for producing the ARA, wherein the method for culturing the microorganisms for producing the ARA comprises the steps of controlling the fermentation temperature to be 20 to 25 DEG C after culturing in a fermentation tank for 100 to 140h; and / or, stopping ventilation or reducing the ventilatory capacity by not less than 50 percent at the 8th to 10th day of the culturing in the fermentation tank. The method for preparing the ARA has the advantages that the yield is high, the purity is high, and the large-scale industrial production of the ARA is facilitated.

The invention discloses probiotics prepared from bacillus coagulans and lactobacillus plantarum as well as a preparation method thereof and belongs to the technical field of microorganisms of feeding stuff. The probiotics is prepared by carrying out solid-state fermentation on matrix by using bacillus coagulans, lactobacillus plantarum and lactobacillus casei, wherein the matrix includes soybean meal, bran and corn flour. The fermentation method has the following advantages that the fermentation cost is reduced, the processing is convenient, the pollution is hardly caused, equipment is simple in structure and easy to operate; and therefore the fermentation method is a probiotics solid fermentation technology which can be widely applied to the field of micro-ecology; the probiotics disclosed by the invention is capable of degrading macromolecule proteins in the soybean meal, the bran and the corn flour into oligopeptides and completely decomposing anti-nutrients, antigen protein and the like in the protein, so that the protein quality and the feeding value of fermented products such as the soybean meal are improved; and the fermented products contain viable bacteria and are capable of effectively adjusting microbial florae of animal intestines, improving production performance and increasing the utilization rate of the feeding stuff.

The invention discloses a Bacillus cereus and application thereof to preparation of nitrite reductase. The Bacillus is isolated from thick broad-bean sauce, and is identified as Bacillus cereus LJ01, which has been preserved in China General Microbiological Culture Collection Center in June 19, 2014 and has a preservation number CGMCC No.9360. The strain LJ01 is used for the preparation of nitrite reductase (NiR); NiR extracted from Bacillus cereus LJ01 has high activity; and conditions for NiR to degrade nitrite are simple. The conditions disclosed by the invention can reduce NiR enzyme activity loss to obtain high purity NiR; and NiR provides a novel method for solving the problem of nitrite in three fields of fermented vegetables, meat products and aquaculture.

The invention provides a production method for bacillus licheniformis with a high sporation rate. According to the production method, no manganese chloride is added into a fermentation medium in a fermentation tank, a sterilized manganese chloride solution is supplemented after fermentation is carried out for 23-25 h, and the volume of the sterilized manganese chloride solution accounts for 0.03-0.04 % of the fermentation volume. As manganese chloride is added in the middle and later stage of fermentation, early-stage growth of the bacillus licheniformis is not affected, the sporation rate of the bacillus licheniformis is increased, the fermentation cycle is shortened, the fermentation production cost is reduced, and better product quality is achieved.

The invention discloses a bio-synthesized gene cluster dis427 of Disorazole Z. The nucleotide sequence of the gene cluster is shown in SEQ ID No.1. The invention further discloses an engineering strain DK1622::Km-Ptet-dis427 used for heterologously expressing Disorazole Z efficiently and established through the gene cluster dis427. The engineering strain is obtained in the mode that Myxococcus xanthus DK1622 is used as an original strain, and the bio-synthesized gene cluster dis427 is integrated on a genome of the original strain with a transposition method. The invention further discloses application of the engineering strain DK1622::Km-Ptet-dis427 in preparation of Disorazole Z. A Disorazole Z bio-synthesis way and an efficient expression method of Disorazole Z in heterologous hosts havehigh research and application value in developing novel anti-tumor or anti-infection medicine and reducing the fermentation production cost.

The invention belongs to the field of fermentation engineering, relates to a method for producing DHA (Docosahexaenoic Acid) by utilizing microorganism fermentation and in particular relates to a method for producing mixed oil containing the DHA by utilizing industrial fermentation of schizochytrium strains. More specifically, the invention relates a method for cultivating microorganisms for producing the DHA, wherein the dissolved oxygen saturation is controlled to be 5 percent to 10 percent from 36h to 60h of a culture process of a fermentation tank, and / or a nitrogen source is not added any more from 36h to 60h of the culture process of the fermentation tank. When the method provided by the invention is used for preparing the DHA, the yield is high and the purity is high; large-scale industrial production of the DHA is facilitated.

The present invention relates to a fermentation production method of C19-C21 long-chain dibasic acids. The present invention discloses a method for producing the C19-C21 long-chain dibasic acids by fermentation of candida tropicalis 10468. The candida tropicalis 10468 can grow under high temperature conditions, can also efficiently convert C19-C21 normal alkanes with different carbon chain lengths, linear saturated fatty acids and linear saturated fatty acid derivatives into the C19-C21 long-chain dibasic acids, achieves high-temperature fermentation and efficient production of the C19-C21 long-chain dibasic acids, and can be used for the fermentation production of the C19-C21 long-chain dibasic acids in practice.

The invention discloses an engineering strain for heterologous expression of a histone deacetylase inhibitor FK228. The engineering strain is named as an engineering strain E264:: R-tdp-dep, and the engineering strain is obtained by using Burkholderia thailanensis E264 [delta] oprABC [delta] tdpDE5kb as an original strain and integrating a combined biosynthetic gene cluster R-tdp-dep on a genome of an original strain through a transposition method. The invention also discloses an application of the engineering strain in preparation of the histone deacetylase inhibitor FK228. Experiments prove that the yield of FK228 produced by the engineering strain E264:: R-tdp-dep reaches 94 mg / L and is nearly 5 times that of a current FK228 industrial production strain, the yield of the FK228 is greatly increased, the fermentation cost is reduced, the fermentation production period is shortened, a foundation is laid for large-scale preparation and development of histone deacetylase inhibitor drugs, The engineering strain has obvious economic value and social benefit.

The invention discloses a method for efficiently introducing L-arabinose isomerase. According to the method, in the inducible expression process that an L-arabinose isomerase recombinant bacterium constructed by lactose operon plasmid pET-22b(+) expresses a targeted protein, enzyme substrate D-galactose is added, so that the effective folding of the L-arabinose isomerase is promoted, the activity expression of the L-arabinose isomerase is efficiently introduced, and the expression of active enzyme is effectively improved. By the method, the expression and the fermentation enzyme activity of the active L-arabinose isomerase by the L-arabinose isomerase recombinant bacterium constructed by the lactose operon plasmid pET-22b(+) are improved, so that the fermentation production cost of the g L-arabinose isomerase can be greatly reduced; and the obtained product L-arabinose isomerase is novel carbohydrase which can catalyze the D-galactose to be synthesized into high value-added functional rare sugar-D-tagatose, and has wide market prospect.

The invention belongs to the field of fermentation engineering and relates to a method for extracting DHA crude oil. Specifically, the method includes the following steps of 1, conducting dehydrationtreatment on microbial fermentation liquid used for generating DHA; 2, flexibly squeezing a product obtained in step 1 to obtain the DHA crude oil. The DHA prepared by means of the method has the advantages of high yield and high purity, and the method is favorable for large-scale industrial production of the DHA.

The invention provides a novel photosynthetic organism hydrogen generation process based on the oscillation-light-filling strategy. Specifically, the invention provides a hydrogen generation method utilizing photosynthetic bacteria. The method comprises the following steps: (1) culturing photosynthetic bacteria capable of generating hydrogen under the nonstatic state and the irradiation of initial incident light with the light intensity I1, wherein the light intensity I1 of the initial incident light satisfies the following conditions: (i) I1>I0, I0 is the optimal light intensity of the initial incident light during static cultivation; (ii) under the light intensity I1, the actual light intensity I1' in at least 70% of area (or volume) of the culture system is larger than or equal to the actual light intensity I0' in the same area (or volume) during static cultivation; and (2) separating the generated hydrogen from the culture system. By using the method of the invention, hydrogen generation efficiency of photosynthetic bacteria can be effectively improved, hydrogen generation period can be shortened and high substrate conversion efficiency can be maintained.

The invention belongs to the field of fermentation engineering, and relates to a method for purifying DHA crude oil. Specifically, the method includes the steps of hydration, decolorization and molecular distillation of the DHA crude oil. When used for preparing DHA, the method ensures a high yield and high purity and is beneficial to large-scale industrial production of DHA.

The invention discloses a pretreatment method for producing butanol by fermentation of bagasse. The pretreatment method comprises the following steps of mechanical grinding, liquid-state hot-water treatment, microwave treatment, microbial decomposition, ammonia treatment and enzymolysis treatment, after the steps are performed, the decomposition products in each step can be mixed and subjected to step-by-step saccharification and fermentation and simultaneous saccharification and fermentation. By the pretreatment method, the contents of major inhibitors furancarboxaldehyde and hydroxymethyl furfural can be controlled below the concentration without affecting the growth of fermentative bacteria and other inhibitors such as glucuronic acid, p-coumaric acid, syringic acid and ferulic acid are not basically produced. Since the detoxification purification treatment of the pretreated decomposition products is not needed and the pretreated decomposition products are directly subjected to biological fermentation to produce butanol, the pretreatment method has the advantages that the production of inhibitors can be controlled, the cost is low, the yield is high and the environment friendliness is achieved.

The invention relates to the field of gene engineering, particularly a high-specific-activity endo-xylanase NPWXynB, and a gene and application thereof. Orthogenesis and site-directed mutagenesis are utilized to perform molecular modification on endo-xylanase XynB derived from rumen fungus Neocallimastix patriciarum to obtain the high-specific-activity xylanase NPWXynB. In the amino acid sequence, the 58th site Y is substituted by F, the 78th site M is substituted by Y, the 94th site Y is substituted by S, the 143rd site V is substituted by L, the 145th site E is substituted by R, and the 182nd site D is substituted by R. The specific activity of the endo-xylanase mutant NPWXynB provided by the invention is 11500 U / mg under the condition of 37 DEG C, which is enhanced by 82.5% as compared with the specific activity of the original endo-xylanase XynB. The maximum enzyme activity of the NPWXynB-gene-containing recombinant engineering bacterium can reach 153000 U / mL, and the production cost is greatly lowered.

The invention provides a novel photosynthetic organism hydrogen generation process based on the oscillation-light-filling strategy. Specifically, the invention provides a hydrogen generation method utilizing photosynthetic bacteria. The method comprises the following steps: (1) culturing photosynthetic bacteria capable of generating hydrogen under the nonstatic state and the irradiation of initial incident light with the light intensity I1, wherein the light intensity I1 of the initial incident light satisfies the following conditions: (i) I1>I0, I0 is the optimal light intensity of the initial incident light during static cultivation; (ii) under the light intensity I1, the actual light intensity I1' in at least 70% of area (or volume) of the culture system is larger than or equal to the actual light intensity I0' in the same area (or volume) during static cultivation; and (2) separating the generated hydrogen from the culture system. By using the method of the invention, hydrogen generation efficiency of photosynthetic bacteria can be effectively improved, hydrogen generation period can be shortened and high substrate conversion efficiency can be maintained.

Provided are an α-amylase AmyL mutant with increased activity, and a coding gene and an application thereof. The amino acid sequence of the mutant is shown in SEQ ID NO. 2. The fermentation enzyme activity of the improved α-amylase strain reached 36900 U / mL in 180 hours, 41.5% higher than the pre-transformed α-amylase production strain.

The invention discloses a pretreatment method for producing butanol by fermentation of bagasse. The pretreatment method comprises the following steps of mechanical grinding, liquid-state hot-water treatment, microwave treatment, microbial decomposition, ammonia treatment and enzymolysis treatment, after the steps are performed, the decomposition products in each step can be mixed and subjected to step-by-step saccharification and fermentation and simultaneous saccharification and fermentation. By the pretreatment method, the contents of major inhibitors furancarboxaldehyde and hydroxymethyl furfural can be controlled below the concentration without affecting the growth of fermentative bacteria and other inhibitors such as glucuronic acid, p-coumaric acid, syringic acid and ferulic acid are not basically produced. Since the detoxification purification treatment of the pretreated decomposition products is not needed and the pretreated decomposition products are directly subjected to biological fermentation to produce butanol, the pretreatment method has the advantages that the production of inhibitors can be controlled, the cost is low, the yield is high and the environment friendliness is achieved.

The invention provides a microbial compound bacterial agent, a microencapsulated compound microecological agent, meat rabbit feed, a preparation method and an application thereof, and relates to the technical field of microorganisms. The microbial compound agent includes Bacillus lentus and Bacillus Veles. It can significantly improve the intestinal micro-ecological environment of animals, increase the conversion rate of meat rabbit feed, and achieve the purpose of rapid weight gain. The microencapsulated composite micro-ecological preparation provided by the present invention selects the microbial composite bacterial agent as the core material, and the wrapped probiotics can be released in the intestinal tract of animals, which can resist adverse environments such as high gastric acid and bile salts in the host body, and are more convenient for transportation and long-term storage. The meat rabbit feed provided by the invention can obviously improve the utilization rate of the meat rabbit feed and shorten the growth period of the meat rabbit.

The invention provides a microorganism fermentation method for arachidonic acid. The microorganism fermentation method comprises the following steps of: inoculating ordinary mortierella strains which is used for producing the arachidonic acid on a PDA (Potato Dextrose Agar) slope; stewing a solid culture medium at a temperature of 80-100 DEG C for 20-30 minutes, and airing to dry and scatter the solid culture medium without clusters, wherein the solid culture medium includes liquid components and solid components, the solid components are selected from one or more of wheat bran, rice and millet, and are mixed with the liquid components at a ratio of 10:7; adding 15-25mL sterile water to a fresh arachidonic acid-producing ordinary mortierella strain slope to wash spores so as to prepare spore suspension, uniformly inoculating the spore suspension into the solid culture medium, cultivating the solid culture medium at a temperature of 24-26 DEG C for 2-3 days as a mother culture medium for primary fermentation; and washing the spores on surfaces of solid seeds by using 200-300ml sterile water to prepare the spore suspension, inoculating the spore suspension into a primary fermentation tank based on an inoculation amount of 5-10% by volume, cultivating the primary fermentation tank at a changed temperature through controlling temperatures before and after the fermentation, and adding auxiliary materials for two times during the fermentation process so as to promote the growth of thallus and synthesis of grease. The microorganism fermentation method has the advantages of shortened period and lowered cost, and can be used for effectively improving fermentation level of the arachidonic acid.

The invention belongs to the technical field of biochemical engineering, and discloses a method for producing 1,3-propanediol through fermentation, which comprises the following steps of: preparing a culture medium; culturing seeds; and fermenting to obtain the 1,3-propanediol. By adding malic acid into a fermentation medium, the final concentration is 0.1-10.0g / L; and the adding time is selectedfrom an initial stage of fermentation to an exponential phase of strains. The added malic acid can promote tricarboxylic acid circulation of thalli, and provide more reducing coenzymes and energy. The method is suitable for the aerobic fermentation process of the 1,3-propanediol. The method has the advantages that: producing strains can be promoted to utilize a substrate of glycerol, the concentration and production strength of the 1,3-propanediol are obviously improved, and the production cost is reduced.

The invention discloses a mutant strain of Zymomonas mobilis tolerant to low pH value and its application. The strain is named PH1-29 in taxonomy, and the preservation number is GDMCC 60260; obtained by repeated selection on low pH medium. The strain can not only ensure normal growth in a low pH environment and ensure good fermentation efficiency, but also can eliminate pollution due to the low pH environment, and can also avoid high-temperature and high-pressure sterilization in the fermentation process, control production costs, and improve fermentation efficiency.

The invention discloses a method for drying mycelia. The method includes the following steps that (1) initial mycelia with the water content of 75%-80% are fed into a distributing bin of microwave hot air composite drying equipment to be mechanically distributed; (2) the distributed mycelia are conveyed into a drying chamber of the microwave hot air composite drying equipment and dried through hot air and microwaves at the same time, and meanwhile moisture in the drying chamber is exhausted through a dehumidifier; after being dried to be with the water content of 20%-25%, the mycelia are discharged, and the discharge temperature is 80-90 DEG C; (3) the mycelia are fed into the drying chamber of the hot air drying equipment to be dried, and meanwhile moisture in the drying chamber is exhausted through the dehumidifier; when dried to be with water content lower than 10%, the mycelia are discharged, and the discharge temperature is 60-70 DEG C; (4) the mycelia are smashed, screened and graded; and (5) materials of different grades are inspected and packaged, and dried finished mycelium are obtained. The process is short in drying period, simple in procedure, high in efficiency and free of pollution.

The invention relates to a fermentation production method of C19-C21 long-chain dibasic acids. The invention discloses a method for fermenting and producing C19-C21 long-chain dibasic acids by using Candida tropicalis 10468. Candida tropicalis 10468 can grow under high temperature conditions and can convert different carbon C19-C21 normal alkanes, straight-chain saturated fatty acids, and straight-chain saturated fatty acid derivatives are efficiently converted into C19-C21 long-chain dibasic acids to achieve high-temperature fermentation and efficient production of C19-C21 long-chain dibasic acids. In practice, it is used for the fermentation production of C19-C21 long-chain dibasic acids.

A method for producing Gamma-glutamyltranspeptidase by using Bacillus subtilis belongs to the biotechnology field of foods. The method carries out the three-stage culture of the slope, the seeds and the fermentation of the producing bacteria Bacillus subtilis SK11.004 of the Gamma-glutamyltranspeptidase; and the Gamma-glutamyltranspeptidase powder is obtained by carrying out separation and purification of fermentation broth. The provided method for producing the Gamma-glutamyltranspeptidase by using the Bacillus subtilis SK 11.004 has small breeding workload, low cost and safe and reliable performances, the method can carry out the liquid fermentation production, the cost is low, the method can be used in large-scale production, and the enzyme activity of the Gamma-glutamyltranspeptidase which is produced by using the Bacillus subtilis can be up to more than 4U / mL. The fermented product is non-toxic and can be directly used for producing a plurality of valuable Gamma-glutamyl compounds, such as L-Gamma-glutamyl-L-dopa, Gamma-glutamyl taurine, Gamma-glutamyl tryptophan, glutathione, theanine, and the like.

The invention belongs to the field of fermentation engineering, and relates to a method for producing DHA by microbial fermentation, in particular to a method for producing mixed oil containing docosahexaenoic acid (DHA) by industrial fermentation using Schizochytrium strains. More specifically, the present invention relates to a method for cultivating microorganisms for producing DHA, wherein: starting from 36-60 hours of fermentor culture, the dissolved oxygen saturation is controlled at 5%-10%, and / or From 36-60 hours of tank culture, no nitrogen source was added. The method of the invention prepares DHA with high yield and high purity, and is beneficial to large-scale industrial production of DHA.

The invention provides a method for producing fatty acid by fermenting lignocellulose ionic liquid hydrolysate. An escherichia coli DQ430 is utilized to regulate the total polysaccharide concentration of hydrolysate to be 10-50 g / L with water under an aerobic condition, fatty acid is produced by fermenting in batches or in fed-batch, and after fermenting for 24-72 hours, the concentration of fatty acid can be up to 3-15 g / L; the utilization rate of sugar is 60-90%, and the production intensity is up to 0.063-0.313g / L<-h>. The method for producing the fatty acid by fermenting the lignocellulose ionic liquid hydrolysate has the outstanding advantages that bamboo powder is used as a raw material to replace expansive glucose and is utilized as a carbon source to product fatty acid through fermentation; regenerated biomass resources are utilized, the environmental friendliness is achieved, and the shortage of petrifaction resource for chemic synthesis of fatty acid can be released.