Method for producing DHA (Docosahexaenoic Acid) by utilizing microorganism fermentation

A technology of microorganisms and fermentation tanks, which is applied in the field of producing mixed oils containing docosahexaenoic acid, which can solve the problems of high deodorization temperature, environmental pollution, and easy production of trans fatty acids, etc.

Active Publication Date: 2017-05-10
INNER MONGOLIA KINGDOMWAY PHARMA LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are inevitably some problems in this process technology, such as: in order to meet the requirement of low acid value in alkali refining, excessive alkali is usually added, and some triglycerides will inevitably be saponified; high COD wastewater produced by alkali refining will pollute the environment; Alkali refining requires high temperature treatment for a long time, which will easily cause the peroxide value and anisidine value of the product to increase; high deodorization temperature and long time will easily produce trans fatty acids and other disadvantages

Method used

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  • Method for producing DHA (Docosahexaenoic Acid) by utilizing microorganism fermentation
  • Method for producing DHA (Docosahexaenoic Acid) by utilizing microorganism fermentation
  • Method for producing DHA (Docosahexaenoic Acid) by utilizing microorganism fermentation

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Experimental program
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Embodiment 1

[0125] Embodiment 1 is the original culture method (does not adopt dissolved oxygen control strategy, nitrogen source control strategy and sub-tank culture strategy); embodiment 2 adopts dissolved oxygen control strategy; embodiment 3 adopts nitrogen source control strategy; embodiment 4 is The strategy of sub-tank cultivation was adopted; in Examples 5-13, the strategy of regulating dissolved oxygen, the strategy of nitrogen source regulation and the strategy of sub-tank cultivation were adopted at the same time.

[0126] In the following examples 1-13, unless otherwise specified, the formula of the seed medium used is: glucose 3%, peptone 1%, yeast powder 0.5%, sea crystal 2%, pH natural (the rest is water). The formula of the fermentation medium is: glucose 12%, peptone 1%, yeast powder 0.5%, sea crystal 2% (the rest is water).

[0127] Example 1: The original culture method (do not adopt dissolved oxygen control strategy, nitrogen source control strategy and sub-tank cul...

Embodiment 2

[0136] Example 2: Dissolved oxygen control strategy for 100m 3 Effect of DHA Fermentation in Fermenter

[0137] The slant-preserved strain of Schizochytrium sp. CGMCC No. 6843 was inserted into a 2L shake flask containing 400 mL of medium, and cultured at a temperature of 25° C. at a rotation speed of 200 rpm for 24 hours to complete the activation culture of the strain. According to the inoculation amount of 0.4%, the shake flask seed solution was connected to the primary seed tank equipped with the sterilized medium, cultivated at a temperature of 28°C, a ventilation rate of 1vvm, a tank pressure of 0.02MPa, and a stirring speed of 50rpm for 30h to complete the primary stage Seed expansion cultivation. According to the inoculation amount of 3%, the seed liquid of the first-level seed tank was connected to the second-level seed tank equipped with the sterilized medium, and the culture temperature was 28 ° C, the ventilation rate was 1 vvm, the tank pressure was 0.02 MPa,...

Embodiment 3

[0143] Embodiment 3: Using nitrogen source control strategy to 100m 3 Effect of DHA Fermentation in Fermenter

[0144] The slant-preserved strain of Schizochytrium sp. CGMCC No. 6843 was inserted into a 2L shake flask containing 400 mL of medium, and cultured at a temperature of 25° C. at a rotation speed of 200 rpm for 24 hours to complete the activation culture of the strain. According to the inoculation amount of 0.4%, the shake flask seed solution was connected to the primary seed tank equipped with the sterilized medium, the cultivation temperature was 28°C, the ventilation rate was 1vvm, the tank pressure was 0.02MPa, and the stirring speed was 50rpm and cultivated for 30h to complete the primary stage Seed expansion cultivation. According to the inoculation amount of 3%, the seed liquid of the first-level seed tank was connected to the second-level seed tank equipped with the sterilized medium, and the culture temperature was 28 ° C, the ventilation rate was 1 vvm,...

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Abstract

The invention belongs to the field of fermentation engineering, relates to a method for producing DHA (Docosahexaenoic Acid) by utilizing microorganism fermentation and in particular relates to a method for producing mixed oil containing the DHA by utilizing industrial fermentation of schizochytrium strains. More specifically, the invention relates a method for cultivating microorganisms for producing the DHA, wherein the dissolved oxygen saturation is controlled to be 5 percent to 10 percent from 36h to 60h of a culture process of a fermentation tank, and / or a nitrogen source is not added any more from 36h to 60h of the culture process of the fermentation tank. When the method provided by the invention is used for preparing the DHA, the yield is high and the purity is high; large-scale industrial production of the DHA is facilitated.

Description

technical field [0001] The invention belongs to the field of fermentation engineering, and relates to a method for producing DHA by microbial fermentation, in particular to a method for producing mixed oil containing docosahexaenoic acid (DHA) by industrial fermentation using Schizochytrium strains. Background technique [0002] DHA, full name docosahexaenoic acid (cis-4,7,10,13,16,19-docosahexaenoic acid, DHA), is a polyunsaturated fatty acid. It is difficult for the human body to synthesize itself and must be ingested from the outside world. DHA is one of the essential fatty acids in the human body. It has important physiological regulation functions and health care functions. When it is deficient, it will cause a series of diseases, including growth retardation, skin abnormalities, scales, infertility, mental retardation, etc. In addition, it is also effective for cardiovascular diseases. Special preventive and therapeutic effects. Relevant studies have also shown that ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64C11B1/06C11B3/10C11B3/12C12R1/645
CPCC11B1/06C11B3/001C12P7/6427
Inventor 王炳荣文昌陈必钦张斌韩雯庞毅李丹詹光煌
Owner INNER MONGOLIA KINGDOMWAY PHARMA LTD
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