125results about How to "Efficient hydrolysis" patented technology

A process for the production of glucose from a pretreated lignocellulosic feedstock is provided. The method comprises enzymatically hydrolyzing the pretreated lignocellulosic feedstock with cellulase enzymes to produce a hydrolyzate slurry comprising glucose and unhydrolyzed cellulose and fermenting the hydrolyzate slurry in a fermentation reaction to produce a fermentation broth comprising alcohol. A process stream is obtained comprising unhydrolyzed cellulose, which is then subjected to a denaturing step, preferably comprising exposing the unhydrolyzed cellulose to elevated temperatures, thereby producing a heat-treated stream comprising the unhydrolyzed cellulose. The heat-treated stream comprising unhydrolyzed cellulose is then further hydrolyzed with cellulase enzymes to hydrolyze the cellulose to glucose.

The invention relates to a method of extracting hemicellulose, cellulose and lignin from wood fiber raw materials and belongs to the field of utilization and technology of biomass resources of agriculture and forestry. In the method, the hemicellulose is extracted from the wood fiber raw materials by using hot water, then the lignin is extracted by using alkaline organic solvent under mild conditions; and residual ingredients rich in the cellulose are further used for enzyme hydrolysis to prepare fermentable sugar solution, thereby achieving full-ingredient utilization of the hemicellulose, cellulose and lignin in the biomass resources of the agriculture and forestry. The method has simple process, and is environmentally friendly, the organic solvent can be recycled, thereby promoting the development of the green agriculture and forestry, and the method has wide social and economic benefits.

The invention relates to a method for producing ethanol or acetone and butanol by taking lignocellulose as a raw material. According to the invention, a wet disc grinding machine-ultrafine grain melting and crushing machine physical method is adopted for pretreating the lignocellulose raw material, so that a cellulose structure wrapped by hemicellulose is opened and a cellulose crystalline structure is simultaneously opened, the method can be used for replacing all acid, alkali and gas explosion treatment methods, the conditions of high temperature and high pressure are not required, no external metal or organic matter such as sulfur is added, the pollution is avoided and the hydrolysis efficiency is high. By adopting the method, no secondary substance inhibiting the growth and the fermentation of yeast, bacteria and anaerobic bacteria is generated, and cellulose, the hemicellulose and lignin are not damaged.

The present invention relates to a method for producing a dairy product using an enzyme having lactase activity.

The invention provides a preparation of a glycosyl microsphere catalyst and a process for hydrolyzing cellulose, belonging to the field of conversion and utilization of a biomass resource and energy resources. The glycosyl microsphere catalyst provided by the invention has a molecular formula of CH0.61O0.57S0.05. The preparation conditions of the catalyst is relatively moderate and the catalyst has a very high catalytic activity; the yield of reducing sugar obtained by hydrolyzing the cellulose in a novel green solvent ion solution is high, and the temperature for the reaction is low and the reaction time is short. Compared with a liquid acid, the reaction process has no pollution and no corrosion, and the catalyst can be circularly utilized for a plurality of times. The process for hydrolyzing the cellulose is a green and effective process. Saccharides for preparing the catalyst are obtained from the natural world and have the advantages of reproducibility, no damages to human health and environment, cheap price and easiness in obtaining. The hydrolysate reducing sugar can be further converted into important platform compounds such as ethanol, 5-hydroxymethylfurfural and the like, and compounds such as bio-fuel and the like, which are capable of replacing traditional petrochemical resources.

The invention relates to an enzyme composition for the high-efficiency decomposition of cellulose, which is a recombinant biologic enzyme capable of effectively decomposing the cellulose under the conditions of high temperature and strong acid. The enzyme composition comprises two enzymes from pyrococcus horikoshii and one auxiliary protein, namely, 1, internally cutting 1,4 glucanase, 2, externally cutting beta-glycuronide and 3, forward folding element protein; the proportion of the three proteins in the enzyme composition is preferably 1:1:0.1-1. The enzyme gene of an amino acid residue sequence of the former two enzymes after being analyzed by deletion and optimized and the other two alpha and beta subgroup genes containing the auxiliary protein (the forward folding element protein) are respectively regulated and controlled by a heat promoter of a temperature sensitive type and are expressed with high efficiency in bacteria; the auxiliary protein has the function of greatly enhancing the activity of the two enzymes; therefore, the cellulose enzyme composition can decompose the cellulose with high efficiency into monosaccharide under the conditions of high temperature and strong acid; and the potential application of the cellulose enzyme composition is the decomposition of cellulose class wheat straws for providing a raw material for the production of biologic petroleum.

The invention discloses a method for preparing furfural, hydroxymethylfurfural and levulinic acid by means of a microchannel reaction device.The method is characterized by comprising the following steps that 1, lignocellulose is utilized for preparing a soluble oligosaccharide solution; 2, oligosaccharide in the soluble oligosaccharide solution is catalyzed by HCl to be converted into furfural or hydroxymethylfurfural or levulinic acid in the microchannel reaction device, wherein during the catalysis process, the molar ratio of soluble oligosaccharide to HCl is 1:(1-3), the temperature of the catalytic reaction ranges from 140 DEG C to 210 DEG C, the flow velocity of the catalytic reaction ranges from 1 ml/min to 6 ml/min, and the retention time of the catalytic reaction ranges from 2 min to 10 min; 3, after the reaction is completed, the reaction fluid is cooled, and furfural, hydroxymethylfurfural and levulinic acid in the reaction fluid are extracted.By means of the method, the conversion rate of pentose is 90%-97%, the highest yield of furfural reaches 92%, the conversion rate of hexose is 83%-92%, the highest yield of 5-HMF is 85%, and the height yield of levulinic acid is 87%.

The invention discloses a preparation method of a compound enzyme preparation for promoting production of methane from kitchen waste through anaerobic fermentation. The preparation method is characterized by comprising the following steps of: 1, preparing formula compositions which comprises liquifying enzyme, saccharifying enzyme, cellulase and lipase; 2, separation: classifying and recovering non-biomass parts in the kitchen waste through a manual or mechanical method, wherein a biomass part enters into a composite enzymatic hydrolysis process; 3, crushing and homogenating: crushing the biomass part obtained after the kitchen waste is separated into paste, and homogenating for later use; 4, composite enzymatic hydrolysis; and 5, anaerobic fermentation. According to the invention, from the aspects of methane anaerobic fermentation microbial metabolism and enzyme activity change rule, through using a compound enzyme preparation formula, the effects of promoting rapid and efficient hydrolysis of the kitchen waste and growth and metabolism of methane fermentation microorganism are achieved, and the purpose of efficient and stable operation of a system for producing methane by the kitchen waste through anaerobic fermentation is further realized.

The invention discloses a bacillus megaterium which is a bacillus megaterium JD4 collected by the China Center for Type Culture Collection (CCTCC) and has a collection number of CCTCC NO: M2016033. The invention also discloses an application of the bacillus megaterium, and an organic fertilizer additive and a biological organic fertilizer prepared by using the bacillus megaterium for fermentation. The bacillus megaterium disclosed by the invention has good protease producing activity, can efficiently degrade organic substances, can be used for preparing biological organic fertilizers, and has good application prospects.

Mutated β-glucuronidase enzymes with enhanced enzymatic activity and thermostability as compared to wild type enzyme are provided. The enzymes of the invention advantageously allow for accurate analysis of bodily samples for the presence of drugs in 30 minutes or less, as compared to the several hours needed using prior enzyme preparations. Methods of using the mutated enzymes for hydrolysis of glucuronide substrates, including opiates and benzodiazepines, are also provided.

The invention provides a hyaluronic acid hydrolase and an encoding sequence thereof, and a method for preparing an oligomeric hyaluronate by using the hyaluronic acid hydrolase. The nucleotide sequence encoding the hyaluronic acid hydrolase provided by the invention can be transferred into engineering bacteria through methods of transduction, transformation, and combination transfer, and the hyaluronic acid hydrolase is efficiently expressed by regulating the expression of the encoding gene, so that an effective way for the production of oligomeric hyaluronic acid is provided. The hyaluronic acid hydrolase is used to hydrolyze hyaluronic acid, the operation is simple, conditions are mild, the efficiency is relatively high, the purity of the obtained oligomeric hyaluronic acid is relativelyhigh, and at the same time the hyaluronic acid hydrolase obtained from a fermentation source has relatively low cost, so that the hyaluronic acid hydrolase is suitable for large-scale industrial production.

The invention discloses a preparation method of raw starch-digesting glucoamylase and application of the raw starch-digesting glucoamylase to raw starch hydrolysis and preparation of ethanol by simultaneous saccharification and fermentation of raw starch. The raw starch-digesting glucoamylase is obtained after codon optimization, and a nucleotide sequence of the raw starch-digesting glucoamylase is shown as any of from A1) to A5): A1), sequence 3 in a sequence table; A2), nucleotides from 1st position to 1878th position in the sequence 3 in the sequence table; A3), DNA molecules shown by nucleotides in 19th position to 1878th position in the sequence 3 in the sequence table; A4), DNA molecules shown by nucleotides in 19th position to 1857th position in the sequence 3 in the sequence table; A5), a protein sequence shown in sequence 4 in a coding sequence table and showing 98% identity with the sequence limited by A1), A2), A3) or A4). Through experiments, it is proven that raw starch-digesting glucoamylase genes can be effectively expressed in a heterologous expression system to obtain the raw starch-digesting glucoamylase rPoGA15A, and the raw starch-digesting glucoamylase rPoGA15A and alpha-amylase act synergistically to hydrolyze the raw starch rapidly and efficiently and prepare the ethanol by simultaneous saccharification and fermentation of the raw starch.

The invention discloses fermentation preparation and application of Chaetomium globosum dextranase and belongs to the technical fields of fermentation technology, enzymic preparations and sugar engineering. A high-yield Chaetomium globosum dextranase fermentation method is determined by optimizing medium components and fermentation conditions through orthogonal test, thereby improving the yield ofChaetomium globosum dextranase up to 698.22 U/mL; when applied to hydrolyzing high-molecular-weight glucan and at a final concentration of 2 U/ml, the Chaetomium globosum dextranase can achieve a high-molecular-weight glucan (T2000) hydrolyzing rate up to 97.9% within 15 minutes; the rate of inhibition of the Chaetomium globosum dextranase on biological membranes formed by streptococcus mutans can reach 97.9%, and the clearance rate of the biological membranes reaches up to 49.07%. The prepared Chaetomium globosum dextranase has a good application prospect in food and sugar industries and thefield of medicine.

The invention discloses a biological preparation method of dehydroepiandrosterone. The preparation method starts from steroid androstenedione ester which is low in price and easy to obtain to finally obtain a target product with the yield up to 94% through a one-step biological enzyme catalytic reaction. In the process, an intermediate A is not accumulated, so that oxidizing impurities caused by instability of the intermediate A cannot be generated. Therefore, high reaction yield and good product quality can be achieved.

The invention discloses a trichoderma koningiopsis strain and application of the trichoderma koningiopsis to the preparation of cellulase. The invention provides the trichoderma koningiopsis strain FCD3-1 capable of being used for preparing the cellulase, and the preservation number of the trichoderma koningiopsis strain FCD3-1 is CGMCC (China General Microbiological Culture Collection Center) No. 6050. The invention also provides a method for preparing the cellulase. The method comprises the following steps that the trichoderma koningiopsis strain FCD3-1 is subjected to liquid fermentation, fermentation products are collected, thalli are removed through centrifugation, obtained fermentation supernatant can be used as a cellulase preparation, the cellulase preparation can be used for efficiently hydrolyzing and crystallizing the cellulose, in addition, the final hydrolysis product is glucose, the cellulase preparation contains high-activity beta-glucosidase, the most proper pH value of the beta-glucosidase is 4.5, the most proper temperature is 60 DEG C, and in addition, better pH tolerance and temperature stability are realized. The cellulase preparation can be used for the lignocellulose hydrolysis.

The invention discloses a nano cerium oxide modified organic and inorganic silane passivation film. The nano cerium oxide modified organic and inorganic silane passivation film is prepared by following raw materials including dodecafluoroheptyl methacrylate, hexafluorobutyl methacrylate, a silane coupling agent KH560, azodiisobutyronitrile, a silane coupling agent KH570, a proper number of silane coupling agents KH858, cerium oxide fiber, sodium dodecyl benzene sulfonate, polyacrylamide, polyacrylamide, silica sol, hexamethyl-disilazane, sodium molybdate, ammonium hexafluorotitanate, molybdenum disulfide, ribonucleoside triphosphote, water soluble acrylic resin, a proper amount of hydrogen peroxide, a proper amount of absolute ethyl alcohol, a proper amount of tetrahydrofuran, a proper amount of dilute nitric acid and a proper amount of deionized water. Prepared passivation liquid is good in stability and can be applicable to surface treatment of steel, zinc and aluminum; a nano level oxide conversion film is formed on a treated workpiece, the uniformity and fineness of the passivation film can be greatly improved, and accordingly the durability and corrosion resisting performance can be achieved.

The invention discloses an alpha-galactosidase gene and application thereof, and relates to the technical field of biology. The alpha-galactosidase gene is suitable for encoding alpha-galactosidase, and a nucleotide sequence is shown as SEQ ID NO:1. The artificially designed alpha-galactosidase gene has the advantages that the high-frequency codon in pichia pastoris is used for replacing the original low-frequency codon, so that the free energy of mRNA (messenger ribonucleic acid) and the complexity of mRNA secondary structure are decreased; by selecting the secondary high-frequency codon, the areas containing AT (adenine and thymine) or GC (guanine and cytosine) in the gene or the protease action site are eliminated, and the expression amount of the artificially synthesized alpha-galactosidase gene in the pichia pastoris cell is obviously increased.

The invention belongs to the field of genetic engineering and specifically relates to a catechol 2,3-dioxygenase gene and an application thereof. The nucleotide sequence of the catechol 2,3-dioxygenase gene is shown as SEQ ID NO.1. The gene expression product catechol 2,3-dioxygenase is capable of effectively hydrolyzing aromatic compound catechol, 4-methyl catechol, catechol, 1,2,4-pyrogallol and2,6-dimethyl-1,3,4-pyrogallol. The catechol 2,3-dioxygenase gene can be applied to the industries of biological remediation for environmental pollution, industrial wastewater treatment and 2-hydroxylfurfural production, not only can solve the environmental pollution of catechol aromatic compounds, but also can acquire great economic benefit.

The crude oil reserves have a calculable time limit. Starting materials containing silicon dioxide are preferably used as raw materials.

The invention provides an organic-inorganic hybridized molybdenum arsenate crystal as well as a preparation method and application thereof. The invention relates to the organic-inorganic hybridized molybdenum arsenate crystal as well as the preparation method and the application thereof, aiming at solving the problems that existing reaction conditions for preparing ethylene glycol through hydrolyzing ethylene carbonate are harsh, pollution is easily caused and a catalyst is not easy to recycle. The preparation method comprises the following steps: mixing (NH4)6Mo7O24.4H2O, C10N2H8, NaAsO2 andCuCl2.2H2O; adding a mixture into distilled water and stirring; sealing and putting into a baking oven for heating; cooling at room temperature; finally, washing with distilled water and drying at room temperature to prepare a product. The organic-inorganic hybridized molybdenum arsenate crystal is used as the catalyst and is used for an ester hydrolysis process under normal pressure; the catalystis easy to separate. The crystal prepared by the preparation method can be used as the catalyst and is used for catalyzing ester hydrolysis; ester is not adsorbed and efficient hydrolysis of the ester under the normal pressure is realized. The organic-inorganic hybridized molybdenum arsenate crystal is applied to the field of preparation of the catalyst.

According to the present invention a miso with good flavor and a processed food containing the miso are provided, wherein the miso and the food are expected to have an repressing effect on an increase in blood pressure or hypotensive effect by continually taking the miso or food for routinely dietary habit. The present invention also provides a method of producing a miso, wherein peptides with ACE inhibitory activity are effectively produced in the miso without lowering the quality in flavor of miso. In an embodiment of the present invention, animal milk is added at a content of 10% during a mixing step, or a fermentation and ripening step.

The invention relates to a thermophilic bacillus DF7 strain. The thermophilic bacillus DF7 strain is preserved in the China General Microbiological Culture Collection Center, with a preservation number of CGMCC No.16459. The strain provided by the invention can efficiently hydrolyze antibiotic bacterial residues under the condition of aerobic fermentation, dissolve out intracellular organic substances, promote subsequent resource utilization, and promote the lysis of E.coli cells.

The invention relates to special lubricating oil for a grinding roller of a vertical mill and a manufacturing method of the special lubricating oil. The special lubricating oil is prepared by taking synthetic oil as base oil, adding a variety of efficient compound additives and refining, and the special lubricating oil can fastest repair abrasion, reduce abrasion and avoid dry friction and high-temperature coking. The product is widely applied to mining, metallurgy, electric power, chemical engineering, cement and other industries for being used on vertical mills, has the advantages of good viscosity-temperature property, strong extreme pressure abrasion resistance, thermal stability, no coking at high temperature and the like, is further relatively cheap, environment-friendly and safe, and can fundamentally solve the problem that the vertical mill has a failure due to poor lubrication.

The invention discloses a method for enzymatic degumming of high-acid-value rice bran oil, and belongs to the technical field of enzyme separation and application. The method comprises the following steps: pretreating high-acid-value rice bran oil by using Lipase G50, and performing enzymatic degumming by using phospholipase; the Lipase G50 (only can act on monoglyceride and diglyceride, and can not act on triglyceride) is used for specifically removing partial glyceride with an emulsifying effect in the high-acid-value rice bran oil; therefore, the wrapped phospholipid in the high-acid-valuerice bran oil is released, the contact probability of phospholipase and phospholipid in the high-acid-value rice bran oil during degumming is increased, the enzymatic degumming reaction rate and efficiency are greatly improved, and the bottleneck problems of low enzymatic degumming rate and low efficiency of the high-acid-value rice bran oil are solved. Meanwhile, after the Lipase G50 is used forpretreating the high-acid-value rice bran oil, the Lipase G50 is enriched in a lower-layer water phase and can be recycled for multiple times.

The invention relates to a geobacillus thermodenitrificans DC8 strain and application thereof, the geobacillus thermodenitrificans DC8 strain is preserved in China General Microbiological Culture Collection Center (CGMCC) on August 30, 2021, and the preservation number is CGMCC No.23318. The strain provided by the invention has the capability of degrading excess sludge to extract protein in a high-temperature environment, and the protein extraction rate can reach 29.7%. Protein in sludge can be effectively utilized by utilizing the strain, the reduction and degradation effect of residual sludge is improved, and the strain has a good application prospect. According to the invention, an efficient bacterium source is provided for the reduction of the residual sludge of a sewage plant, the functional application of the geobacillus thermodenitrificans is widened, and the geobacillus thermodenitrificans has a relatively high practical value.