15results about How to "Efficient hydrolysis" patented technology

The invention discloses a process method for preparing fish collagen tripeptide and application of the fish collagen tripeptide, and belongs to the technical field of collagen peptide preparation processes. According to the method, the raw material fish skin is subjected to pretreatment and pretreatment, and then the pretreated fish skin is subjected to two times of enzymolysis treatment, so that efficient hydrolysis of fish skin collagen is realized, and a collagen peptide product with small molecular weight is obtained. Firstly, neutral protease and papain are used for carrying out primary enzymolysis on fish skin, then a microbial fermentation agent and an activity accelerant are used for carrying out deep fermentation enzymolysis treatment, peptide fragments can be effectively further cut, the yield of small molecule peptide fragments is increased, and the biological activity is improved. The method is simple, the process is easy to control, environmental pollution is avoided, the enzymolysis time is short, the enzymolysis speed and the extraction rate of the collagen peptide can be increased, and the collagen peptide obtained through the method is low in molecular weight, good in biological activity and easier to popularize and apply.

The invention relates to a synthesis method of room-temperature vulcanized methyl phenyl silicone rubber, and belongs to the field of preparation of high polymer materials. The preparation method comprises the following steps: adding alpha, omega-dihydroxy polydimethyldiphenylsiloxane with high phenyl content and low polymerization degree, dimethyl cyclosiloxane and an alkaline resin catalyst into a reaction kettle, stirring, heating and reacting; after the reaction is finished, removing low-boiling-point substances in vacuum to obtain room-temperature vulcanized methyl phenyl silicone rubber; the preparation method comprises the following steps: preparing D201 ion exchange resin (quaternary ammonium salt type), 1-allyl cyclopropanesulfonyl chloride, zinc chloride, bis (1-vinyl imidazole-2-) ketone, mercaptoethyl guanidine dihydrobromide, triethylamine and dichloromethane, so as to obtain guanidine-doped D201 ion exchange resin; loading guanidine-doped D201 ion exchange resin into an ion exchange column for transformation to obtain an alkaline resin catalyst; the room-temperature vulcanized methyl phenyl silicone rubber prepared by the invention has excellent mechanical properties.

The invention discloses a method for preparing xylobiose and xylotriose from agricultural and forestry waste bamboo sawdust, and belongs to the technical field of agricultural and forestry biomass degradation and conversion. The method comprises the following steps: firstly, adjusting the pH value of a lactic acid solution, then mixing the raw material bamboo sawdust with the lactic acid solution, hydrolyzing, and filtering to obtain a treatment solution containing xylooligosaccharide and filter residues; and then mixing the treating fluid with xylanase to carry out enzymolysis reaction, and further decomposing xylan and xylooligosaccharide with high polymerization degree in the aqueous solution into xylobiose and xylotriose. The xylobiose and the xylotriose are obtained by adjusting the pH value of the lactic acid solution in combination with an endo-xylanase hydrolysis method, so that the xylooligosaccharide with higher biological activity is prepared, and the xylooligosaccharide can be used as a functional feed additive and can also be used as a functional food additive through subsequent treatment.

This invention discloses a chitin endonuclease gene, recombinant vector, recombinant bacteria, recombinant enzyme, preparation method, and applications of high expression of Poria cocos chitin endonuclease. The nucleotide sequence of the gene is shown in SEQ ID No. 1. The nucleotide sequence shown in SEQ ID No. 1 can be used to achieve high-level recombinant secretory expression of the target protein in Pichia pastoris host bacteria using a Pichia pastoris inducible expression vector. Under high-density fermentation conditions, the total secretory expression level of the target protein can reach 10.7 g / L. The recombinant enzyme is purified by DEAE ion exchange chromatography, and this recombinant enzyme can efficiently hydrolyze colloidal chitin to produce chitin oligosaccharides. The recombinant Poria cocos chitin endonuclease prepared by this invention has important application value and broad application prospects in the preparation of functional chitosan oligosaccharides, antibacterial, antitumor, environmental protection, and agriculture.

The invention relates to the technical field of vegetable oil processing, in particular to a method for preparing strong-flavor soybean oil through enzymolysis and aroma enhancement by means of extrusion puffing, and aims to provide the method for preparing the strong-flavor soybean oil through enzymolysis and aroma enhancement by means of extrusion puffing. According to the method, efficient and low-energy-consumption production of the soybean oil is achieved through a series of innovative process steps, neutral cellulase and flavourzyme are adopted for conducting enzymolysis on soybean embryo pieces, and amino acid, polypeptide, reducing sugar and the like are generated by hydrolyzing a substrate through an enzyme preparation in the hydrolysis process and serve as reaction substrates; maillard reaction is carried out under the action of high temperature in the extrusion process to generate aroma, aroma components are absorbed in the oil to obtain the strong-aroma soybean oil, and the method is not only suitable for production, but also suitable for aroma enhancement processes of other vegetable oil, and has relatively high practicability and popularization value.

The application provides a recombinant polymyxin enzyme and a preparation and application method thereof, an amino acid sequence of the recombinant polymyxin enzyme is shown as SEQ ID NO. 1, and the recombinant polymyxin enzyme is obtained through site-directed mutation on an amino acid sequence of a wild-type polymyxin enzyme derived from Brevibacillus laterosporus. The recombinant polymyxin enzyme can specifically recognize and cut a peptide bond between a tripeptide side chain and a cyclic heptapeptide ring in a polymyxin structure and a peptide bond between threonine and diaminobutyric acid inside the cyclic heptapeptide ring, and further can specifically degrade or neutralize the recombinant polymyxin enzyme of polymyxin.

The invention discloses a preparation method and application of an enzyme preparation for enzymatically hydrolyzing bitter substances in citrus juice. Using Aspergillus tabingensis UA13 isolated in the early stage of the laboratory and mutagenized by atmospheric and room temperature plasma (ARTP) as the starting strain, limonin in lemon peel powder is used as an inducer to induce enzyme production, and the fermentation enzyme production process of the strain and the substrate affinity of the enzyme are studied. The results show that: in a 1 L fermentor medium, under the optimal conditions of limonin addition amount of 4.0 mg / L, inoculation amount of 10% (v / v), initial pH value of 5.0, stirring speed of 250 rpm, and fermentation at 30 °C, the highest limonin debittering enzyme activity can reach 48.52 U / mL. The results of the study on the substrate affinity of the enzyme for limonin show that the Km value of the limonin debittering enzyme for limonin is 0.622 mmol / L, indicating strong affinity. At the same time, the enzyme can still efficiently hydrolyze other bitter substances such as naringin and hesperidin, has good heat resistance and acid resistance, and has very good application effects in the citrus juice processing industry.

This invention discloses a method and application for treating moldy corn to synthesize polyhydroxy fatty acids (PHA) using a compound enzyme preparation, relating to the fields of fermentation engineering and waste resource utilization technology. The method for treating moldy corn to synthesize PHA using a compound enzyme preparation includes the following steps: 1) screening and culturing highly efficient halophilic bacteria as inoculum strains for open-enzyme PHA synthesis; 2) pretreating moldy corn with a compound enzyme preparation composed of α-amylase, β-amylase, and glucoamylase; 3) using the moldy corn hydrolysate as a fermentation carbon source, inoculating with PHA-producing halophilic bacteria for open-enzyme fermentation. This invention constructs a complete process system of specific halophilic bacteria screening – moldy corn enzymatic pretreatment – ​​open-enzyme fermentation to synthesize PHA, realizing the resource utilization and high-value utilization of moldy corn. Compared with existing technologies, it has significant economic, environmental, and industrial value, with outstanding comprehensive beneficial effects.

The invention relates to the technical field of anaerobic digestion of crop straw raw materials, and particularly discloses a method for strengthening anaerobic methanation of crop straw based on biological micro-aerobic pretreatment combined with a microbial electrolysis cell. According to the method, crop straw is placed in a closed system added with calcium peroxide (CaO2) and inoculated sludge for biological micro-aerobic pretreatment. A micro-aerobic environment created by CaO2 is utilized to stimulate facultative hydrolytic bacteria to enrich and secrete a large amount of hydrolase, and the lignocellulose structure of the straw is efficiently cracked. After pretreatment, the system is transferred into a microbial electrolysis cell reactor, inhibition of residual oxidizing substances in pretreatment on methanogenic archaea is effectively eliminated by utilizing a cathode reduction effect, and meanwhile, electron transfer and methanogenic metabolism paths of the system are enhanced. Through a coupling strategy of'biological micro-aerobic pretreatment + microbial electrolysis cell ', synergistic improvement of hydrolysis and methanation processes is realized, and an innovative and feasible solution is provided for efficient anaerobic digestion of crop straws to produce methane.

The invention provides a method for simultaneously recovering neopentyl glycol, sodium butyrate and n-butyl alcohol, according to the method, polyol wastewater containing natural sodium hydroxide is used as the only hydrolysis alkali source of neopentyl glycol heavy component waste liquid, and efficient hydrolysis of esters in the neopentyl glycol heavy component waste liquid can be realized without adding any additional alkali agent; neopentyl glycol, sodium butyrate and n-butyl alcohol in the hydrolysate are simultaneously recovered through ozone oxidation, distillation, rectification and the like, so that an integrated process of'alkali source internal circulation-multi-component synergistic conversion-gradient separation and purification 'is realized. In the invention, the recovery rate of neopentyl glycol is greater than or equal to 85%; meanwhile, the purity of neopentyl glycol is greater than or equal to 97%, the purity of sodium butyrate is greater than or equal to 95%, and the purity of n-butyl alcohol is greater than or equal to 98%, all reach the industrial-grade raw material standard, and can be directly sold or reused for production.

The invention provides lactobacillus plantarum with high beta-glucosidase activity and application of the lactobacillus plantarum, and belongs to the technical field of microbial fermentation. The lactobacillus plantarum with the high beta-glucosidase activity, provided by the invention, comprises lactobacillus plantarum (Lactobacillus plantarum) LZU-J-LZ1-2 or Lactobacillus plantarum (Lactobacillus plantarum) LZU-J-QA5-2, and the lactobacillus plantarum with the high beta-glucosidase activity, the beta-glucosidase activity, the beta-glucosidase activity, the beta-glucosidase activity and the beta-glucosidase activity, and the beta-glucosidase activity and the beta-glucosidase activity of the beta-glucosidase activity and the beta-glucosidase activity of the beta-glucosidase activity are both high. The lactobacillus plantarum with high beta-glucosidase activity also has acid resistance, salt resistance and gastric juice and intestinal juice tolerance, has strong gastrointestinal tract survival potential, provides precious strain resources for developing efficient and safe multifunctional microbial preparations, and has important industrial application value.

The invention discloses application of xylanase, and belongs to the technical field of bioengineering. According to preferred codons of bacillus subtilis, a wild type xylanase (GH11 family) coding gene which is secreted by Cellulostridium morganii and is not represented is designed, and a genetic engineering technology is utilized, so that the wild type xylanase is subjected to recombinant expression in the bacillus subtilis. Through verification, the separated xylanase still has xylan hydrolysis activity under the conditions of high temperature (close to 100 DEG C) and strong alkali (pH 9-10.5), and shows good heat resistance and alkali resistance; and meanwhile, more than 60% of enzyme activity can still be maintained in a high-temperature (100 DEG C) environment within 1 hour, and the strain has relatively high thermal stability and has a good application prospect in extreme environments such as industrial production and the like.

The present application relates to the field of enzyme engineering and genetic engineering, and in particular to alpha-L-rhamnosidase BtRha78A-W435 mutant, and a preparation method and application thereof. The amino acid sequence of the mutant has one amino acid mutation with the alpha-L-rhamnosidase BtRha78A shown in SEQ ID NO. 1, and the mutation is that the tryptophan at the 435th position is mutated into lysine and arginine, respectively. The mutant of the present application has higher catalytic activity and catalytic efficiency on rutin, and has high substrate selectivity, high specificity and efficient hydrolysis of rutin, and provides theoretical guidance for industrialization of realizing green and efficient precise production of isorhamnetin with higher biological efficacy and higher bioavailability by hydrolyzing cheap and abundant rutin.

The invention relates to a preparation method of hydrogenated starch hydrolysate, which comprises the following steps: (1) mixing plant starch with water to obtain gelatinized plant starch; (2) mixing the gelatinized plant starch with a compound enzyme, carrying out an enzymolysis reaction, and carrying out deactivation treatment on the enzyme after the reaction to obtain a polysaccharide slurry; (3) decolorizing the polysaccharide slurry obtained in the step (2); (4) carrying out hydrotreatment on the decolorized polysaccharide slurry to obtain a crude hydrogenated starch hydrolysate; (5) filtering and concentrating the crude hydrogenated starch hydrolysate obtained in the step (4) to obtain hydrogenated starch hydrolysate; wherein the compound enzyme in the step (2) comprises gamma-amylase, isoamylase, glycogen phosphorylase and alpha-amylase. According to the preparation method, gelatinized plant starch is comprehensively and efficiently hydrolyzed, and the starch is decomposed into products with proper sizes and structures, so that the prepared hydrogenated starch hydrolysate has excellent stability and moisturizing performance.

The invention discloses heat-resistant alpha-galactosidase and a method for enzymatically hydrolyzing raffinose. The heat-resistant alpha-galactosidase has a specific amino acid sequence (SEQ ID NO. 1) and can be obtained through a genetic engineering means. The preparation method comprises the following steps: optimizing a codon, cloning into a recombinant plasmid pET20b-TstGal, transforming escherichia coli BL21 (DE3), carrying out IPTG (isopropyl-beta-d-thiogalactoside) induced expression, purifying and the like. The optimized induction conditions are as follows: the IPTG concentration is 0.1 mmol / L, the OD600 value is 1.0, the temperature is 42 DEG C, and the time is 5 hours. The obtained alpha-galactosidase is suitable for enzymatic hydrolysis of raffinose, and preferably, the enzymolysis condition is a citric acid-disodium hydrogen phosphate buffer solution system with the raffinose concentration of 0.1 mol / L, the enzyme concentration of 1.5 U / mL, the temperature of 70 DEG C and the pH value of 6.5. The alpha-galactosidase provided by the invention has heat resistance, the preparation method is efficient, enzymolysis conditions are optimized, a powerful tool enzyme is provided for hydrolysis of raffinose, and the alpha-galactosidase has a wide application prospect.