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Method for enzymatic degumming of high-acid-value rice bran oil

A high-acid value rice bran oil and degumming technology, which is applied in the direction of oil/fat refining, fat production, etc., can solve the problems of low efficiency and slow degumming rate of high-acid value rice bran oil by enzymatic method, so as to achieve slow solution speed and increase contact probability. , the effect of improving the reaction rate and efficiency

Active Publication Date: 2020-06-30
SHAANXI UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the above problems, the object of the present invention is to provide a method for enzymatic degumming of high acid value rice bran oil, which greatly improves the reaction rate and efficiency of enzymatic degumming, Solved the bottleneck problem of slow rate and low efficiency of high acid value rice bran oil enzymatic degumming

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Accurately weigh 200g high-acid value rice bran crude oil (acid value 50.92mg KOH / g) and place it in a 500mL conical flask with a stopper; heat the rice bran crude oil to 40°C in a glycerin bath constant temperature magnetic stirrer, then add 10mL to remove Ionized water and 60U / g (U / w, relative to the quality of the substrate oil) Lipase G "Amano" 50; continue to stir and react at 500rpm and 40°C for 30min; Centrifuge at 12,000rpm for 2min, collect the upper oil layer and remove water by rotary evaporation at 0.09Mpa, 70°C; the lower aqueous phase is collected for the next batch of reactions. Accurately weigh 100g of Lipase G "Amano" 50 pretreated high-acid rice bran crude oil and place it in a 500mL conical flask with a stopper; heat the pretreated high-acid value rice bran crude oil to 70°C, add 120 μL of citric acid solution with a mass concentration of 45% and homogenize with a high-speed homogenizer at a speed of 10,000 rpm for 1 min; then continue to stir and rea...

Embodiment 2

[0032]Accurately weigh 200g high-acid value rice bran crude oil (acid value 50.92mg KOH / g) and place it in a 500mL conical flask with a stopper; heat the rice bran crude oil to 35°C in a glycerin bath constant temperature magnetic stirrer, then add 10mL to remove Ionized water and 100U / g (U / w, relative to the quality of the substrate oil) Lipase G "Amano" 50; continue to stir the reaction at 350rpm and 35°C for 15min; after the reaction, the above mixture is quickly centrifuged Centrifuge at 12,000rpm for 2min, collect the upper oil layer and remove water by rotary evaporation at 0.09Mpa, 70°C; the lower aqueous phase is collected for the next batch of reactions. Accurately weigh 100g of Lipase G "Amano" 50 pretreated high-acid rice bran crude oil and place it in a 500mL conical flask with a stopper; heat the pretreated high-acid value rice bran crude oil to 70°C, add 120 μL of citric acid solution with a mass concentration of 45% and homogenize with a high-speed homogenizer a...

Embodiment 3

[0034] Accurately weigh 200g high-acid value rice bran crude oil (acid value 50.92mg KOH / g) and place it in a 500mL conical flask with a stopper; heat the rice bran crude oil to 25°C in a glycerin bath constant temperature magnetic stirrer, then add 10mL to remove Ionized water and 40U / g (U / w, relative to the quality of the substrate oil) Lipase SMG1 (refer to the preparation method described in the document J.Mol.Catal.B-Enzym., 2012,77:87-91 to prepare Lipase Freeze-dried enzyme powder of SMG1); continue to stir the reaction at 400rpm and 25°C for 1h; after the reaction, quickly centrifuge the above mixture with a centrifuge at 12,000rpm for 2min, collect the upper oil layer and store it at 0.09Mpa at 70°C The water was removed by rotary evaporation under certain conditions; the lower aqueous phase was collected for the next batch of reactions. Accurately weigh 100g of high acid value crude rice bran oil pretreated by Lipase SMG1, and place it in a 500mL conical flask with a...

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PUM

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Abstract

The invention discloses a method for enzymatic degumming of high-acid-value rice bran oil, and belongs to the technical field of enzyme separation and application. The method comprises the following steps: pretreating high-acid-value rice bran oil by using Lipase G50, and performing enzymatic degumming by using phospholipase; the Lipase G50 (only can act on monoglyceride and diglyceride, and can not act on triglyceride) is used for specifically removing partial glyceride with an emulsifying effect in the high-acid-value rice bran oil; therefore, the wrapped phospholipid in the high-acid-valuerice bran oil is released, the contact probability of phospholipase and phospholipid in the high-acid-value rice bran oil during degumming is increased, the enzymatic degumming reaction rate and efficiency are greatly improved, and the bottleneck problems of low enzymatic degumming rate and low efficiency of the high-acid-value rice bran oil are solved. Meanwhile, after the Lipase G50 is used forpretreating the high-acid-value rice bran oil, the Lipase G50 is enriched in a lower-layer water phase and can be recycled for multiple times.

Description

technical field [0001] The invention belongs to the technical field of separation and application of enzymes, and in particular relates to an enzymatic degumming method for high acid value rice bran oil. Background technique [0002] The oil content of rice bran is 15-23%, which is equivalent to soybean, and it is a good source of oil. The rice bran oil prepared by pressing or leaching rice bran has a balanced fatty acid composition and contains more lipid companions such as oryzanol, phytosterol and vitamin E, etc., and has high nutritional value. At present, the annual output of rice bran in my country is about 15 million tons, but the utilization rate of rice bran in my country is less than 20%. Most of the rice bran is discarded as livestock feed or waste, which not only causes a lot of waste of resources, but also pollutes the environment. [0003] Due to the small and scattered rice processing enterprises in my country, and rice bran is prone to rancidity and deterior...

Claims

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Application Information

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IPC IPC(8): C11B3/00C11B3/16C11B3/12
CPCC11B3/003C11B3/001C11B3/16C11B3/12
Inventor 李道明刘看看刘宁崔俊杰
Owner SHAANXI UNIV OF SCI & TECH
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