Preparation method of compound enzyme preparation for promoting production of methane from kitchen waste through anaerobic fermentation
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A technology of kitchen waste and compound enzyme preparation, which is used in fermentation, waste fuel and other directions to achieve the effect of promoting fast, stable operation and efficient hydrolysis
Active Publication Date: 2013-04-03
INST OF PLASMA PHYSICS CHINESE ACAD OF SCI +1
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However, there is no report on the method of using a specific compound enzyme formula to improve the efficiency and yield of biogas production from kitchen waste by first enzymolysis and then anaerobic fermentation. There is no report
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Embodiment 1
[0022] Take 50 g of the kitchen waste with a grease content of 4.5%, sorted, pulverized and homogenized, and carry out the following two groups of experiments according to the compound enzyme preparation formula 1:
[0023] Experimental group I: first add lipase to enzymatically hydrolyze the oil in the kitchen waste. Add lipase according to the amount of 180 U per gram of dry matter of the kitchen waste biomass, adjust the pH value to 6.5, place on a shaker at 100 rpm, keep a constant temperature of 40°C, and perform enzymatic hydrolysis for 36 hours.
[0024] Then add liquefaction enzyme, glucoamylase, cellulase, etc. to enzymatically hydrolyze other biomass components in kitchen waste. Add liquefaction enzyme, glucoamylase, and cellulase according to the amount of 8U, 150U, and 50U per gram of dry matter of the kitchen waste biomass, adjust the pH value to 5.5, place it on a shaker at 80-120 rpm, and keep it at 60°C Constant temperature, enzymatic hydrolysis time 4h.
[0...
Embodiment 2
[0031] Take 0.5% oil content, sorted, pulverized and homogenized kitchen waste 50g each, according to the compound enzyme preparation formula 2, carry out the following 2 groups of experiments:
[0032] Experimental group I: first add liquefaction enzyme, glucoamylase, cellulase, etc. to enzymatically hydrolyze the biomass components in kitchen waste, and then perform anaerobic fermentation to produce biogas. Add liquefaction enzyme, glucoamylase, and cellulase according to the amount of 8U, 150U, and 50U per gram of dry matter of the kitchen waste biomass, adjust the pH value to 5.5, place it on a shaker at 80-120 rpm, and keep it at 60°C Constant temperature, enzymatic hydrolysis time 4h. Then add 200g of biogas fermentation anaerobic sludge cultivated by the laboratory to the sample after enzymolysis as inoculum, add 1L laboratory-made constant temperature anaerobic fermentation device together, add water to 750mL, mix well, and keep the fermentation temperature at 35°C. T...
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Abstract
The invention discloses a preparation method of a compound enzyme preparation for promoting production of methane from kitchen waste through anaerobic fermentation. The preparation method is characterized by comprising the following steps of: 1, preparing formula compositions which comprises liquifying enzyme, saccharifying enzyme, cellulase and lipase; 2, separation: classifying and recovering non-biomass parts in the kitchen waste through a manual or mechanical method, wherein a biomass part enters into a composite enzymatic hydrolysis process; 3, crushing and homogenating: crushing the biomass part obtained after the kitchen waste is separated into paste, and homogenating for later use; 4, composite enzymatic hydrolysis; and 5, anaerobic fermentation. According to the invention, from the aspects of methane anaerobic fermentation microbial metabolism and enzyme activity change rule, through using a compound enzyme preparation formula, the effects of promoting rapid and efficient hydrolysis of the kitchen waste and growth and metabolism of methane fermentation microorganism are achieved, and the purpose of efficient and stable operation of a system for producing methane by the kitchen waste through anaerobic fermentation is further realized.
Description
technical field [0001] The invention mainly relates to a preparation method of a compound enzyme preparation, in particular to a preparation method of a compound enzyme preparation for promoting anaerobic fermentation of kitchen waste to produce biogas. Background technique [0002] Kitchen waste has the dual attributes of typical garbage and resources. It contains a large amount of starch, sugar, protein, oil, cellulose and other nutrients, as well as nitrogen, phosphorus, potassium, calcium and various trace elements. Under the cultural background, the amount of urban kitchen waste resources is huge. At the same time, due to the disorderly management of most urban kitchen waste, food safety and environmental pollution problems such as "gutter oil" and "garbage pigs" have become a major issue. focus of social attention. [0003] For the resource utilization of kitchen waste, there are currently three main ways: processing animal feed, making fertilizer, and energy utilizat...
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