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A method for improving dewatering performance of citric acid wastewater sludge by using Penicillium oxalicum

A technology of citric acid wastewater and Penicillium oxalate, applied in water/sludge/sewage treatment, dehydration/drying/concentrated sludge treatment, sludge treatment, etc., can solve the problem of biological conditioning that has not been reported and the source of bacteria has not been specified , long culture cycle and other problems, to achieve the effect of short separation and purification cycle, easy operation, and improved dehydration performance

Active Publication Date: 2020-04-28
BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing Chinese Patent Publication No. CN107176781A discloses a method for improving sludge dewatering performance by using yellow-blue fungus mycelium, but the limitation of the method is that the cultivation time of mycelium needs 4-10 days, and the cultivation The cycle is long, and the source of the bacteria is not specified, which is not conducive to application
Moreover, most of these studies focus on municipal sludge, and there is no report on bioconditioning of food fermentation sludge with high organic matter such as citric acid sludge

Method used

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  • A method for improving dewatering performance of citric acid wastewater sludge by using Penicillium oxalicum
  • A method for improving dewatering performance of citric acid wastewater sludge by using Penicillium oxalicum
  • A method for improving dewatering performance of citric acid wastewater sludge by using Penicillium oxalicum

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Experimental program
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Effect test

Embodiment 1

[0037] Embodiment 1 for testing sludge sample

[0038] A method for improving the dehydration performance of citric acid sludge by using Penicillium oxalicum according to the present invention. During the specific implementation and operation, the sludge samples for testing were taken from the secondary sedimentation process of the oxidation ditch treatment process of the sewage treatment workshop of a citric acid production enterprise in Shandong. Pool return sludge. The enterprise produces 60t of absolute dry sludge every day. The sludge treatment method is to add PAM coagulation after concentration. The basic characteristics of the tested sludge are listed in Table 1.

[0039] Table 1 Basic characteristics of the sludge samples tested

[0040] Moisture content (%) Capillary water absorption time (s) Sludge specific resistance (×10 14 m / kg)

Embodiment 2

[0041] The separation and purification of embodiment 2 sludge filamentous fungi

[0042] Dilute the citric acid wastewater sludge sample by 10-10 5 After different concentration gradients, they were inoculated in Sabouraud medium by the coating plate method, cultured in a constant temperature incubator at 25-30°C for 2-3 days, and a variety of fungal colonies were isolated and picked by the three-point inoculation method. Colonies were grown in petri dishes. Repeat the inoculation for 3 to 5 times, isolate and purify the fungi with different colony forms, inoculate the fungi with different colony characteristics on the slant medium, and store them in a refrigerator at 4°C.

Embodiment 3

[0043] Example 3 Screening and Identification of Filamentous Fungi

[0044] Analyze the morphology and growth characteristics of the purified fungi, screen out filamentous fungi with fast growth and developed mycelia, and then conduct organic degradation experiments on them, including gelatin liquefaction, cellulose hydrolysis, starch hydrolysis, and screen out characteristics Obvious filamentous fungus, identified by 18SrDNA gene sequence and morphology, the morphology under the microscope is as follows figure 1 As shown, it was identified as Penicillium oxalicum.

[0045] (1) Gelatin liquefaction experiment

[0046] Medium configuration: glucose 20g, peptone 5.0g, gelatin 200g, dissolved in 1000mL distilled water for sterilization, take 10mL sterilized medium solution in a test tube. The fungus was inoculated on the culture medium and cultured at 25°C and 180rpm for several days. Put the blank control group and the fungus-inoculated group into a low-temperature environment ...

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Abstract

The invention discloses a method for improving dehydration performance of citric acid sludge by using penicillium oxalicum and belongs to the field of treatment of wastewater sludge of citric acid industry. According to the method, the penicillium oxalicum with an extracellular polymer degradation function is separated, purified and screened out from the citric acid wastewater sludge, a fungal spore suspension with a concentration of 1.0*10<7> / mL to 1.0*10<8> / mL is prepared from the penicillium oxalicum, and then, a Martin liquid culture medium is inoculated with the fungal spore suspension for culture for 2 to 4 days, so that stable mycelia are formed. The mycelia are added into the citric acid wastewater sludge with a total suspended solid content of 1% to 4% according to a volume ratioof 5% to 20%, culture is carried out for 2 to 4 days after uniform mixing, the sludge specific resistance SRF and capillary water absorption time CST of the citric acid wastewater sludge can be separately lowered to (1-6)*10<13>m / kg and 6-12 seconds from (1-3)*10<14>m / kg and 25-43 seconds, and the dehydration performance is remarkably improved.

Description

technical field [0001] The invention relates to the field of citric acid industry wastewater sludge treatment and disposal, in particular to a method for improving the dehydration performance of citric acid wastewater sludge by using Penicillium oxalicum. Background technique [0002] Citric acid is mainly fermented, extracted and refined from corn, dried potato and other starchy materials. The process will produce a large amount of high-concentration and refractory organic wastewater. Therefore, compared with municipal sludge, the residual sludge produced has higher The protein content of the sludge is extremely high, and the dehydration performance is very poor. The moisture content of this type of sludge is still as high as 85% to 90% after dehydration. Since the high water content brings great difficulties to its subsequent treatment, disposal and transportation, it is necessary to pretreat the sludge before dewatering to improve the dewatering performance. [0003] At ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C02F11/02C02F11/147C02F11/122C02F11/123
CPCC02F11/02C02F11/12
Inventor 董黎明李影影汪苹郑心愿李杨杨
Owner BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY
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