64 results about "Sphaerotrichia divaricata" patented technology

The invention discloses lactobacillus rhamnosus. A strain (lactobacillus rhamnosus) LR863 is preserved in China General Microbiological Culture Collection Center (CGMCC) and the preservation number isCGMCC No. 14410. The lactobacillus rhamnosus disclosed by the invention has remarkable bacterium-inhibition and sterilization effects on oral pathogenic microorganisms including streptococcus mutans,streptococcus gordonii, porphyromonas gingivalis, fusobacterium nucleatum, actinobacillus actinomycetemcomitans and the like, and has the effects of reducing formation of dental plaque and reducing occurrence and development of oral diseases including tooth decay, periodontitis and the like. A lactobacillus rhamnosus preparation disclosed by the invention can comprise viable bacterium thalli of the lactobacillus rhamnosus, dead bacterium thalli of the lactobacillus rhamnosus, metabolic products of the lactobacillus rhamnosus and intracellular extract, is an oral probiotics preparation which has wide application and a remarkable effect, and can be widely used as an additive of liquid-state foods, solid-state foods, colloidal foods, oral hygiene products and oral treatment drugs.

The invention discloses lactobacillus plantarum KLDS1001 and application of the lactobacillus plantarum KLDS1001 to inhibition of streptococcus mutans, belonging to the technical field of microorganisms. The lactobacillus plantarum KLDS1001 provided by the invention is collected in China general microbiological culture collection center (CGMCC) with the address of institute of microbiology of Chinese academy of sciences, 1# yard, beichen road west, chaoyang district, Beijing; the collection number is CGMCC No.9066; the collection date is April 16th, 2014. The lactobacillus plantarum KLDS1001 has an inhibition effect on the streptococcus mutans, can serve as an additive to be added to health drugs and such oral health products as chewing gum and is used for oral health; the lactobacillus plantarum KLDS1001 can serve as one of ingredients of oral cleaning products to be added into toothpaste and mouth wash for cleaning the oral cavity and reducing the number of streptococcus mutans in the oral cavity; a probiotic therapy method is developed for preventing and treating oral diseases such as decayed teeth.

The present invention provides a vaccine composition for dental caries caused by S. mutans infection, where the vaccine composition comprises an antigen derived from a surface protein PAc of S. mutans and an adjuvant derived from flagellin. The present invention further provides methods for preparing the vaccine composition. The present invention also provides methods for preventing or curing dental caries caused by S. mutans by administrating to a subject the vaccine composition.

The invention discloses an anti-caries polypeptide capable of preventing bacteria and promoting remineralization, a polypeptide derivative or a polypeptide pharmaceutical salt thereof. The anti-caries polypeptide contains an amino acid sequence a and an amino acid sequence b; the amino acid sequence a is Xn, wherein X is used for representing S, T, Y, K, R, N, or Q, n is used for representing the amino acid repeat times, and is an integer selected from 5 to 8; the amino acid sequence b is of a alpha double helix structure, is positively charged, and contains 8 to 12 amino acids, and the hydrophilic amino acids and hydrophobic amino acid in the amino acid sequence b appear alternately. The functional anti-caries polypeptide capable of preventing cariogenic bacteria and promoting remineralization is capable of inhibiting growth of streptococcus mutans, inhibiting formation of biological membrane of streptococcus mutans, reducing the metabolism capability of formed biological membrane of streptococcus mutans, killing viable bacteria in the formed biological membrane of streptococcus mutans, promoting remineralization of demineralization tooth enamel, and reducing caries depth and mineral loss of demineralization tooth enamel, and possesses excellent stability, and no obvious cytotoxicity.

The invention discloses ab analysis method of rejection capability of lactobacillus plantarum metabolite and application of the lactobacillus plantarum metabolite. The effective extract of lactobacillus plantarum FB-T9 contains micromolecular bacteriocin substances and hydrogen peroxide ingredients, so that bacteriostatic activity for streptococcus mutans in floating state is provided and relatively bacteriostatic activity can be provided under biological membrane state to destroy the integrity of the biological membrane structure. The lactobacillus plantarum FB-T9 is applied to fermented food or pharmaceutical compositions containing metabolite of the lactobacillus plantarum FB-T9, is used for preventing, decreasing or treating tooth decay and has wide application prospect.

The invention relates to a preparation method and process for antibacterial modification of ceramic whiskers and in particular provides a preparation method for fusion-coating of zinc oxide nanoparticles having different patterns on the surface of hydroxyapatite whiskers. The preparation method is characterized by adding dropwise an ethanol solution of a zinc salt into a beaker in which an ethanol solution of hydroxyapatite whiskers is held, performing stirring, rotating, ultrasonic treatment, drying and calcination procedures to make zinc oxide-hydroxyapatite whiskers. The ceramic whiskers have antibacterial effects. The zinc oxide nanoparticles have good antibacterial effects and the antibacterial rate against Streptococcus mutans reaches (93.58 plus or minus 5.95) percent; and the hydroxyapatite whiskers have high strength. After modification, zinc oxide nanoparticles, nanospheres, nanorods, microspheres and diamond-shape units are uniformly distributed on the surface of the hydroxyapatite whiskers by fusion coating, the particle size is 30 nm to 400 nm, the surface area and the roughness are greatly improved, and the mechanical embedment force between the zinc oxide nanoparticle and the matrix is increased. Therefore, the zinc oxide-modified hydroxyapatite whisker can serve as an ideal reinforcing material with antibacterial effect.

The present invention discloses a nutritional composition for improving oral health. The nutritional composition comprises the following raw materials in parts by weight: 5-30 parts of probiotics, 20-80 parts of prebiotics and 0.1-2 parts of epigallocatechin gallate; the probiotics are selected from at least one of lactobacillus rhamnosus, lactobacillus reuteri, lactobacillus helveticus, and lactobacillus salivarius; and the prebiotics are selected from at least one of inulin, oligofructose, galactooligosaccharide, xylooligosaccharide, stachyose, oligoisomaltose, raffinose, trehalose, and lactitol. At the same time, the present invention also provides a use of the nutritional composition in preparations of oral health-care products. The provided nutritional composition can inhibit colonization of caries inducing streptococcus mutans, reduces adhesion of subgingival gingiva pathogenic bacteria, inhibits bad breath, promotes the proliferation of the oral probiotics, prevents the occurrence and development of dental erosion and oral cancers, and can effectively solve the oral problems caused by alcoholism, smoking, staying up late, irregular diet, long-term consumption of acidic substances, etc.

The invention discloses a probiotics toothpaste composition capable of improving the health of oral cavity. The composition comprises a first toothpaste component and a second toothpaste component; wherein the first toothpaste component comprises probiotics and mannan oligosaccharide, and the second toothpaste component comprises enzymes, mannatide, and a diester type surfactant; and the first toothpaste component and the second toothpaste component are individually packed, and the first toothpaste component and the second toothpaste component are mixed and blended when being used on the teeth. Mannan oligosaccharide and degradation product of mannatide can provide carbon sources for the growth of probiotics, the pathogenic bacteria in oral cavity cannot utilize mannan oligosaccharide, moreover, mannan oligosaccharide can absorb pathogenic bacteria so as to starve the pathogenic bacteria; at the same time, alkaline polypeptide in degradation product of mannatide can cut off glycan chain of cell walls of bacteria; and the diester type surfactant has a strong cleaning and stain-removing performance, and is capable of effectively remove the residual bacterium bodies. After application, the number of pathogenic bacteria especially streptococcus mutans is greatly reduced, and the health of oral cavity is prominently improved.

The invention discloses a method capable of inhibiting a streptococcus mutans and candida albicans double-bacteria biofilm and belongs to the technical field of microorganisms. At different stages offorming the double-bacteria biofilm, lactobacillus plantarum CCFM8724 can inhibit the double-bacteria biofilm in vitro, exopolysaccharide amount and pathogenic bacteria number in the biofilm can be reduced remarkably when the double-bacteria biofilm is allowed to act at different times, 1.6mg/mL of lysozyme can be tolerated, film forming performance is poor at different sucrose concentrations andlower than below 0.80, and it shows that lactobacillus plantarum cannot replace cariogenic bacteria to form a cariogenic biofilm. The method has efficacy of reducing oral cariogenic bacterial plaque and preventing and treating decayed teeth and is expected to open a new functional market for preventing and treating decayed teeth through probiotics.

The invention belongs to the biological medicine field, and in particular relates to anti-variation streptococcus polypeptide, application and a preparation method thereof. The method is characterized in that the gene clone technique is adopted to establish MBP, Colicin Ia channel domain and CSP fudion gene, and obtain high efficiency expression in the Ia. The invention also discloses a ribonucleotide sequence for encoding recombinant polypeptide, and recombinant plasmid. The antibiotic polypeptide uses cariogenic Streptococcus mutans to forms an ion channel directly on a cell membrane of a target cell to achieve the bactericidal aim.

The invention discloses fermentation preparation and application of Chaetomium globosum dextranase and belongs to the technical fields of fermentation technology, enzymic preparations and sugar engineering. A high-yield Chaetomium globosum dextranase fermentation method is determined by optimizing medium components and fermentation conditions through orthogonal test, thereby improving the yield ofChaetomium globosum dextranase up to 698.22 U/mL; when applied to hydrolyzing high-molecular-weight glucan and at a final concentration of 2 U/ml, the Chaetomium globosum dextranase can achieve a high-molecular-weight glucan (T2000) hydrolyzing rate up to 97.9% within 15 minutes; the rate of inhibition of the Chaetomium globosum dextranase on biological membranes formed by streptococcus mutans can reach 97.9%, and the clearance rate of the biological membranes reaches up to 49.07%. The prepared Chaetomium globosum dextranase has a good application prospect in food and sugar industries and thefield of medicine.

The invention discloses corynebacterium glutamicum capable of increasing lysine yield and a constructing method of the corynebacterium glutamicum, and belongs to the technical field of genetic engineering. A genetic engineering method is adopted for substituting IDH (isocitrate dehydrogenase) coding gene icdCg in corynebacterium glutamicum RG for IDH coding gene icdSm in streptococcus mutans, andaccordingly, affinity of IDH to different redox cofactors is regulated, the problem of intracellular redox imbalance in the synthesis process of L-lysine is solved, and the L-lysine accumulation capacity of the strain is improved. Tank (5L fermentation tank) experiments show that L-lysine accumulation amount of the recombinant bacterium reaches 121.4 g/L. Redox cofactor affinity of IDH in corynebacterium glutamicum is successfully changed, problem of intracellular redox imbalance during synthesis of L-lysine is solved, and a new idea for breeding the L-Lysine high-yield strain is provided.

The invention provides a bergenia crassifolia extract and an extracting method and medical application in oral diseases. Through a resazurin dyeing method and an XTT method, the extract of bergenia crassifolia is subjected to bacteriostatic activity and inhibitory biological film activity study, the extract of bergenia crassifolia has an obvious inhibitory effect on streptococcus mutans, streptococcus sanguis, streptococcus salivarius, lactobacillus acidophilus, porphyromonas gingivalis, fusobacterium nucleatum, actinobacillus actinomycetemcomitan and biological film of the fungi, has a better inhibitory effect on streptococcus mutans, porphyromonas gingivalis and the biological film of the fungi and can be used for preparing daily necessities and medicine for preventing and treating the oral diseases.

The invention discloses a production process for a probiotic periodontal care gel. The production process comprises the following steps: preparation of a solution I: heating purified water, then adding sodium benzoate, cetylpyridinium chloride, sodium fluoride, sucralose, noncrystalline sorbitol, nisin and brilliant blue and carrying out stirring for 20 to 25 min; preparation of a solution II: heating hydrogenated castor oil RH40 and propylene glycol to a fusion state, adding peppermint essence and carrying out uniform mixing under stirring so as to obtain the solution II; preparation of a solution III: uniformly mixing PEG-400, xanthan gum and sodium alginate in a ratio prescribed in a formula under stirring so as to obtain the solution III; and preparation of the probiotic periodontal care gel: adding the solution II into the solution I, carrying out uniform mixing under stirring, then adding the solution III, carrying out stirring for 25 to 30 min so as to form a semi-finished product, taking a sample for laboratory test so as to obtain a semi-finished cetylpyridinium chloride product, carrying out canning when the semi-finished cetylpyridinium chloride product is determined to be qualified and carrying out warehousing after a finished product is determined to be qualified, wherein if cetylpyridinium chloride is insufficient, cetylpyridinium chloride is added, a sample is taken for detection until the semi-finished product is qualified and then canning is carried out, and if the finished product is determined to be unqualified, the finished product is discarded. Lactobacillus can reinforce inhibitory effect of sodium fluoride on Streptococcus mutans, reduce accumulation of dental plaques, prevent gingivitis and carious teeth and improve the balance of microfloras in the oral cavity.

The invention belongs to the technical field of pharmaceutical biology, and relates to an Fmoc chemosynthesis peptide, in particular to an anti-oral cariogenic bacteria polypeptide Pm1 and a preparation method. The preparation method comprises the following steps that: by finding the active center of a natural polypeptide pleurocidin, certain antibacterial and bactericidal capacities on common oral cariogenic bacteria are realized; on the basis of determining the active center, amino acid deletion and replacement are carried out on the active center of the natural polypeptide pleurocidin to form a novel antibacterial peptide with bactericidal capacity; the sequence of the novel antibacterial peptide Pm1 is GWGRFFKKWWRVGKRVGK; the common oral cariogenic bacteria are streptococcus mutans according to the preparation method; the common oral cariogenic bacteria are streptococcus sobrinus according to the preparation method; and the common oral cariogenic bacteria are streptococcus sanguis according to the preparation method. The anti-oral cariogenic bacteria polypeptide Pm1 overcomes the defects that the natural antibacterial peptide is too long, and the synthesis cost is relatively higher.

The present invention relates to an oral cavity spray for inhibiting pathogenic bacteria in oral cavity, and applications thereof, wherein the oral cavity spray contains, by weight, 5-10% of Romaine lettuce polysaccharide, 1-2% of sodium bicarbonate, 1-2.5% of sodium benzoate, 1-2.5% of vitamin C, 5-10% of peppermint oil and lemon oil, 3-6% of hydroxypropylmethylcellulose, hyaluronic acid and xanthan gum, and the balance of water. According to the present invention, the bacterial coating spraying experiment results show that the oral cavity spray can effectively inhibit the activities of Streptococcus mutans and Streptococcus sobrinus, such that the oral cavity spray can effectively attached to the tooth medium so as to inhibit oral cavity disease pathogenic bacteria.

The invention provides a bacteriostatic toothpaste containing bamboo juice. The bacteriostatic toothpaste comprises the following components by weight percent: 0.5-30% of bamboo juice, 12-60% of a friction agent, 10-70% of a moisturizing agent, 0.5-2.5% of a binder, 1.0-3.0% of a foaming agent, 0.1-0.5% of a sweetener, 0-0.8% of a preservative, 0.6-3.0% of essence, 0-0.5% of an assistant, and the balance deionized water. The bacteriostatic toothpaste containing bamboo juice provided by the invention has bacteriostasis rates of 98.5%, 99% and 99.9% respectively on staphylococcus aureus, pseudomonas aeruginosa and streptococcus mutans, and achieves efficient bacteriostasis.

The invention discloses a preparation method and application of a quinoxaline amide compound used as a streptococcus mutans biological membrane inhibitor and relates to the technical field of bacteriostatic drug screening. The inhibitor consists of compounds 2-(4-methoxy-phenyl)-N-(3-{[2-(4-methoxy-phenyl) ethyl] imido}-1,4-dihydro-2-quinoxaline subunit) ethylamine and dimethyl sulfoxide (DMSO). The preparation method comprises the following steps: (1) dissolving 50mg of powdery 2-(4-methoxy-phenyl)-N-(3-{[2-(4-methoxy-phenyl) ethyl] imido}-1,4-dihydro-2-quinoxaline subunit) ethylamine in 1mL of a dimethyl sulfoxide (DMSO) solvent to prepare an inhibitor mother solution of which the initial concentration is 50mg/mL; (2) performing gradient dilution on the inhibitor mother solution by using dimethyl sulfoxide (DMSO), and adding into a brain heart infusion (BHI) liquid culture medium to make sure that the final concentrations of the inhibitor are respectively 0.05mu g/mL, 0.5mu g/mL, 5mu g/mL and 50mu g/mL and the final concentrations of the solvent DMSO are all 1% (v/v). Application tests show that the inhibitor can be used as an oral mouth streptococcus mutans biological membrane inhibitor and a candidate targeted drug for a novel anti-caries strategy.

The invention discloses a chemiluminiscence detection kit of streptococcus mutans and an application method of the chemiluminiscence detection kit. The kit comprises immune globulin G coupling magnetic beads, enzyme-labeled vancomycin, an enzymatic chemiluminiscence substrate, a standard streptococcus mutans suspension, a buffer solution and washing liquid. The application method is characterized in that the vancomycin which is simple, cheap and stable is used as the molecular-recognition reagent, the vancomycin is used along with the specific molecular-recognition reagent immune globulin G to form a double-site molecular recognition mode, and the double-site molecular recognition mode is combined with the enrichment and separation technology and the enzymatic chemiluminiscence technology to perform whole-cell detection of the streptococcus mutans. The detection kit is fast in detection, convenient, accurate, specific, sensitive, stable and cheap, is hopefully applicable to the field detection and fast screening of the streptococcus mutans, and is capable of providing a powerful technical support platform for the streptococcus mutans detection in fields such as clinical diagnosis, food safety and environment detection.

The invention relates to the technical field of plant extraction and application, in particular to an extraction method of a cyperus rotundus extract and toothpaste and mouth wash containing the cyperus rotundus extract. The extraction method of the cyperus rotundus extract comprises the following steps of (1) ultrasonic-microwave cooperated extraction; (2) concentration; (3) column chromatography separation; (4) concentration, and vacuum freeze drying, so as to obtain light yellow or light brown powder, namely the cyperus rotundus extract. The preparation method has the advantages that the pure natural traditional Chinese medicine of cyperus rotundus is extracted and purified, so that the obtained cyperus rotundus extract has an obvious inhibiting function on streptococcus mutans and porphyromonas gingivalis; the cyperus rotundus extract can be used as an active ingredient and be added into oral care articles for preventing and controlling tooth pain and gingival bleeding, such as the toothpaste, the mouth wash, tooth powder, throat lozenge and gum, the obvious functions of pain relieving and bleeding stopping are realized, the prevention function is realized, and the comprehensive health of oral cavity is maintained.

The invention discloses an application of an oxazolone compound as a streptococcus mutans biofilm inhibitor, which belongs to the field of screening technologies of antibacterial drugs. The chemical name of the compound is 2-(4-chlorphenyl)-4-{[(6-methyl-2-pyridyl) amino] methylene}-1,3-oxazole-5((tetrahydro)-ketone, and an inhibitor formed by dissolving the oxazolone compound in DMSO can inhibit the formation and maturation of streptococcus mutans biofilms. The inhibitor has the advantages of small molecular weight, simple structure, good specificity and the like, can significantly inhibit the formation and maturation of biofilms of streptococcus mutans-a main pathogenic bacterium of caries, but has an unobvious influence on streptococcus mutans in a floating state. The inhibitor has an anti-caries potential and can be used as a candidate targeted drug of novel anti-caries strategies.

The invention relates to an extract of edible fungus roots. The extract is prepared through the following steps: drying the edible fungus roots, then, carrying out powder grinding, carrying out screening to obtain fungus root powder, and carrying out sealed preservation on the fungus root powder for later use; adding the fungus root powder into an extraction solvent, carrying out stirring at room temperature, then, carrying out filtration, carrying out freeze drying on filtrate so as to obtain the extract of the edible fungus roots, and preserving the extract at the temperature of -20 DEG C. According to the extract, by adopting the waste edible fungus roots as a raw material, the added value of industrial production of edible fungi is increased, and the profit of enterprises is increased. The prepared extract of the edible fungus roots can be used for effectively inhibiting the growth of oral cavity streptococcus mutans and the formation of biomembranes, the minimal inhibitory concentration is 10mg/ml, the minimal bactericidal concentration is 20mg/ml, and the action of inhibiting the growth of the streptococcus mutans and the formation of the biomembranes presents concentration-time dependence.

The invention discloses a CRISPR-B (Clustered Regularly Interspaced Short Palindromic Repeats-B) gene editing method for a gtfB site of specific targeting streptococcus mutans and application. The gene sequence is shown as SEQ ID NO. 1; the gene editing method comprises the following steps: step 1: connecting a lactic dehydrogenase promoter Ldhp in front of the gene sequence to form Ldhp+CRISPR-B;step 2: connecting the Ldhp+CRISPR-B to a linear plasmid pDL278 through enzyme digestion and connection, so as to form a CRISPR editing vector; step 3: taking an upstream sequence of a target gene gtfB, knocked out by polymerase chain reaction amplification, as upstream; step 4, taking a downstream sequence of a target gene gtfC, knocked out by the polymerase chain reaction amplification, as downstream; step 5: connecting the upstream obtained step 3 and the downstream obtained by step 4 to form a homologous recombination template HR-BC; step 6: converting the CRISPR editing vector obtained by step 2 and the homologous recombination template HR-BC obtained by step 5 into the streptococcus mutans, so as to simultaneously edit the gtfB and the gtfC. By adopting the CRISPR-B gene editing method disclosed by the invention, the synthesis of streptococcus mutans extracellular polysaccharides and the formation of a biological film can be inhibited to the greatest extent.