Analysis method of rejection capability of lactobacillus plantarum metabolite and application of lactobacillus plantarum metabolite

A technology of metabolite inhibition and Lactobacillus plantarum, applied in the field of microorganisms, can solve the problems of biofilm inhibition of Streptococcus mutans without lactic acid bacteria, and achieve the effect of maintaining oral health

Inactive Publication Date: 2015-04-29
JIANGNAN UNIV
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  • Description
  • Claims
  • Application Information

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  • Analysis method of rejection capability of lactobacillus plantarum metabolite and application of lactobacillus plantarum metabolite
  • Analysis method of rejection capability of lactobacillus plantarum metabolite and application of lactobacillus plantarum metabolite
  • Analysis method of rejection capability of lactobacillus plantarum metabolite and application of lactobacillus plantarum metabolite

Examples

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Embodiment 1

[0020] Embodiment 1: Experiment of the inhibitory effect of metabolites of Lactobacillus plantarum FB-T9 on planktonic Streptococcus mutans

[0021] The Streptococcus mutans strain used in this example is ATCC 25175 (obtained from the Microorganism Collection Center, Chinese Academy of Sciences). The strain was cultured in Tryptone Soy Broth (TSBY) supplemented with 0.6% yeast extract. TSBY medium is well known to those skilled in the art, and it contains tryptone, soybean peptone, yeast extract, sodium chloride, glucose and dipotassium hydrogen phosphate, with a pH of 7.1-7.5. Streptococcus mutans cultivated overnight was adjusted to OD with fresh TSBY medium 600 0.5, the number of colonies is about 1×10 8 cfu / ml.

[0022]Inoculate Lactobacillus plantarum FB-T9 strain in MRS medium with 2% inoculation amount, and culture it statically at 37°C. At the end of the stationary phase, collect the culture solution, centrifuge at 12000rpm at 4°C for 20min, take the supernatant, an...

Embodiment 2

[0025] Example 2: Preliminary exploration experiment on antibacterial substances of Lactobacillus plantarum FB-T9

[0026] Take 1ml of the fermentation supernatant of lactic acid bacteria, and treat them in water baths at 37°C, 60°C, 80°C, and 100°C for 20 minutes respectively, set up a blank control, and test the antibacterial activity, so as to explore the effect of heat treatment on the activity of antibacterial substances; 1.0mol / L sodium hydroxide solution and 1.0mol / L hydrochloric acid solution respectively adjust the pH value of the supernatant to pH3.0, 4.0, 5.0, 6.0, 6.5, 7.0, take each 1ml of the adjusted supernatant, Filtrate and test the antibacterial activity, so as to explore the influence of pH on the activity of antibacterial substances; take four parts of lactic acid bacteria fermentation supernatant, adjust the pH to 8.0, 6.0, and 7.0 respectively, add trypsin, papain, and proteinase K in sequence, Make the final concentration 1mg / mL, bathe in water at 37°C f...

Embodiment 3

[0038] Example 3: Effect experiment of metabolites of Lactobacillus plantarum FB-T9 on biofilm formation of Streptococcus mutans

[0039] The formation of early dental plaque biofilm can basically be divided into bacterial colonization (0-6h), bacterial adhesion (6-12h), biofilm basic structure formation (12-24h) and mature biofilm formation (24-48h). these four main stages. Therefore in this embodiment, at the initial stage of the bacterial colonization stage (0h), the initial stage of the bacterial adhesion stage (6h), the initial stage of the formation of the basic structure of the biofilm (12h), after the formation of the basic structure of the biofilm (24h) and mature After the formation of the biofilm structure (at 48h), the metabolites of Lactobacillus plantarum FB-T9 were used to mediate.

[0040] (a) Effect on biofilm formation of Streptococcus mutans

[0041] Firstly, the bacterial concentration was prepared to be 1×10 5 cfu / ml of Streptococcus mutans suspension. ...

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Abstract

The invention discloses ab analysis method of rejection capability of lactobacillus plantarum metabolite and application of the lactobacillus plantarum metabolite. The effective extract of lactobacillus plantarum FB-T9 contains micromolecular bacteriocin substances and hydrogen peroxide ingredients, so that bacteriostatic activity for streptococcus mutans in floating state is provided and relatively bacteriostatic activity can be provided under biological membrane state to destroy the integrity of the biological membrane structure. The lactobacillus plantarum FB-T9 is applied to fermented food or pharmaceutical compositions containing metabolite of the lactobacillus plantarum FB-T9, is used for preventing, decreasing or treating tooth decay and has wide application prospect.

Description

technical field [0001] The invention relates to a method for analyzing the inhibitory properties of selected Lactobacillus plantarum metabolites to Streptococcus mutans biofilm, the metabolites and components of the Lactobacillus can reduce the number of Streptococcus mutans in the oral cavity by inhibiting the activity, and are used for The invention relates to preventing or treating dental caries, belonging to the technical field of microorganisms. Background technique [0002] Caries is a common bacterial infectious disease in humans, which can cause progressive destruction of tooth hard tissue, resulting in dental caries (also known as "tooth decay" or "tooth decay"), which seriously endangers oral health. The resolution of the 60th World Health Assembly pointed out that the prevention of oral diseases and the promotion of oral health must be included in the comprehensive prevention and treatment of chronic diseases. With the development of oral microbiology, immunology...

Claims

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Application Information

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IPC IPC(8): C12Q1/02A61K35/747A61P1/02C12R1/25C12R1/46
Inventor 陈卫张秋香姚沛琳王刚田丰伟刘小鸣赵国忠赵建新张白曦张灏郭敏
Owner JIANGNAN UNIV
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