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Application of canarium album extract in preparation of medicaments, foods or daily necessities for preventing and treating decayed teeth

A technology of green olives and extracts, which is applied in the field of application of green olive extracts in the preparation of medicines, food or daily necessities for the prevention and treatment of dental caries, which can solve the problems that green olive extracts cannot inhibit oral anaerobic bacteria, and achieve convenient prevention and treatment Effect

Active Publication Date: 2013-05-22
LOGISTICS UNIV OF CAPF
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

But so far there is no report on the inhibition of oral anaerobic bacteria by green olive extract

Method used

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  • Application of canarium album extract in preparation of medicaments, foods or daily necessities for preventing and treating decayed teeth
  • Application of canarium album extract in preparation of medicaments, foods or daily necessities for preventing and treating decayed teeth
  • Application of canarium album extract in preparation of medicaments, foods or daily necessities for preventing and treating decayed teeth

Examples

Experimental program
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Effect test

preparation example Construction

[0030] The preparation steps of the green olive extract are as follows: taking green olive branches, leaves or fruits as raw materials, carrying out reflux extraction with water or ethanol aqueous solution, filtering, concentrating the filtrate under reduced pressure to obtain a concentrate, and drying to obtain the green olive extract.

[0031] The preparation parameters of the green olive extracts of Examples 1-14 are shown in Table 1.

[0032] Table 1

[0033]

Embodiment 15

[0034] Example 15 Green olive extract inhibits in vitro caries-inducing Streptococcus mutans, Streptococcus distalis and Lactobacillus test

[0035] Streptococcus mutans CGMCC12500 was purchased from China General Microorganism Culture Collection and Management Center.

[0036] Streptococcus sobrinus ATCC27351 was purchased from Beijing Beinan Chuanglian Biotechnology Research Institute.

[0037] Lactobacillus sp.ACCC11026 was purchased from Beijing Beina Chuanglian Biotechnology Research Institute.

[0038] Test method: Dissolve plant extracts in TPY liquid medium by doubling dilution method, prepare different concentrations of medicinal solutions, and set up blank control tubes (only drug solution and medium) and no drug control tubes (only drug-added control tubes) Bacterial solution and medium) each one. Draw 50 μL of bacterial suspension and add them to the experimental tube and the untreated control tube respectively, place them in an anaerobic incubator, and incubate ...

Embodiment 16

[0042] Example 16 Determination of the effect of green olive extract on the adhesion of Streptococcus mutans.

[0043] Test method: Inoculate Streptococcus mutans strains recovered for 48 hours in TPY liquid medium, culture anaerobically at 80% N2, 10% CO2, 10% H2, and 37°C for 18 hours, and after smear inspection shows pure culture, adjust The final concentration of the bacterial suspension reaches 5×107 CFU / mL, mix 1 part of the bacterial suspension with 10 parts of TPY liquid medium containing green olive extract and inoculate it in a petri dish, set up 3 parallel petri dishes for each sample experimental group . 80% N2, 10% CO2, 10% H2, anaerobic culture at 37°C for 48 hours, discard the supernatant after culture, then quantify the TPY liquid medium, blow and blow to make the bacteria adhered to the glass surface of the petri dish resuspended , TPY medium was used as a blank, and the results of bacterial adhesion and drug action were determined by turbidimetry. Experimen...

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Abstract

The invention discloses an application of a canarium album extract in preparation of medicaments, foods or daily necessities for preventing and treating decayed teeth. Experiments prove that the canarium album extract has an obvious function of inhibiting streptococcus mutans, streptococcus sobrinus and lactobacillus inducing the decayed teeth, can inhibit the adhesivity of the streptococcus mutans, streptococcus sobrinus and lactobacillus, and has a function of inhibiting the acidogenicity of the streptococcus mutans, streptococcus sobrinus and lactobacillus, so that the canarium album extract is very suitable for preventing and treating the decayed teeth. The canarium album extract is particularly suitable to be prepared into various medicament dosage forms or various foods and daily necessities, and is convenient to prevent and treat the decayed teeth.

Description

technical field [0001] The invention relates to the application of a green olive extract in the preparation of medicines, food or daily necessities for preventing and treating dental caries. Background technique [0002] The plant extract involved in the present invention is from the plant extract of green olive which is abundant in my country. The green olive tree Canarium album (Lour.) Raeusch. is a kind of economic fruit tree planted in parts of southern my country. Its mature and dry fruit is oval and blue in color. It is also known as green fruit, green seed, white olive, green olive, etc. The effective active ingredients are olive polyphenols, which have the functions of clearing heat, relieving throat, promoting body fluid, detoxification, immune regulation, lowering blood pressure, lowering blood fat, and scavenging free radicals. It is used to treat sore throat, cough, polydipsia, fish and crab poisoning Wait [1,2 ], its yield is huge and its nutritional value is ri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/32A61K8/97A61K9/12A61P1/02A23L1/29A61Q11/00A61K131/00A23L33/00
Inventor 刘岱琳延慧君陈虹董晓青马卫俊赵艳敏高颖
Owner LOGISTICS UNIV OF CAPF
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