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Anti-oral cariogenic bacteria polypeptide Pm1 and preparation method

An antibacterial polypeptide and cariogenic bacteria technology, applied in the field of medicine and biology, can solve the problems of high synthesis cost and excessive length of natural antibacterial peptides.

Inactive Publication Date: 2012-07-04
倪龙兴
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The object of the present invention is to provide an anti-oral cariogenic bacteria polypeptide Pm1 and its preparation method to overcome the problems of excessive length of natural antibacterial peptide and relatively high synthesis cost

Method used

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  • Anti-oral cariogenic bacteria polypeptide Pm1 and preparation method
  • Anti-oral cariogenic bacteria polypeptide Pm1 and preparation method
  • Anti-oral cariogenic bacteria polypeptide Pm1 and preparation method

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Experimental program
Comparison scheme
Effect test

Embodiment

[0024] figure 1 It is the α-helical structure software prediction map of polypeptides Pm11 and Pm14.

[0025] figure 2 is an electron micrograph of peptide-treated bacteria. A, normal S. mutans; B, Pm11-treated S. mutans; C, Pm14-treated S. mutans; D, normal blood S. mutans; E, Pm11-treated S. mutans; F, Pm14-treated S. mutans Streptococcus mutans. It can be seen that the surface of the bacteria treated with the polypeptide changes, and the phenomenon of porous or bacterial disintegration and shrinkage appears.

[0026] 1. Design and synthesis of peptides

[0027] 1.1 Design of peptides

[0028] The polypeptide is designed by intercepting the active center of pleurocidin and deleting and replacing amino acids. The physical properties of the designed peptides are shown in Table 1. The parameters of the α-helical structure are shown in Table 1, and the schematic diagram is shown in Figure II .

[0029]

[0030]

[0031] 1.2 Fmoc method to synthesize peptides.

[...

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Abstract

The invention belongs to the technical field of pharmaceutical biology, and relates to an Fmoc chemosynthesis peptide, in particular to an anti-oral cariogenic bacteria polypeptide Pm1 and a preparation method. The preparation method comprises the following steps that: by finding the active center of a natural polypeptide pleurocidin, certain antibacterial and bactericidal capacities on common oral cariogenic bacteria are realized; on the basis of determining the active center, amino acid deletion and replacement are carried out on the active center of the natural polypeptide pleurocidin to form a novel antibacterial peptide with bactericidal capacity; the sequence of the novel antibacterial peptide Pm1 is GWGRFFKKWWRVGKRVGK; the common oral cariogenic bacteria are streptococcus mutans according to the preparation method; the common oral cariogenic bacteria are streptococcus sobrinus according to the preparation method; and the common oral cariogenic bacteria are streptococcus sanguis according to the preparation method. The anti-oral cariogenic bacteria polypeptide Pm1 overcomes the defects that the natural antibacterial peptide is too long, and the synthesis cost is relatively higher.

Description

technical field [0001] The invention belongs to the technical field of medicine and biology, and relates to an Fmoc chemically synthesized polypeptide, that is, a polypeptide Pm1 for resisting oral cariogenic bacteria and a preparation method thereof. The polypeptide has the ability of resisting oral cariogenic bacteria, especially bacteria of the genus Streptococcus. Background technique [0002] Although a lot of manpower and material resources have been invested, dental caries is still one of the three major non-communicable diseases in the world. Caries is a chronic and progressive bacterial infectious disease that occurs in dental hard tissues under the influence of various factors in the internal and external environment of the oral cavity. Streptococcus mutans and Streptococcus farus are the main cariogenic bacteria. In 1946, McClure and Hewitt confirmed that penicillin can inhibit the occurrence of dental caries in rats. The main mechanism is to inhibit the synthesi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/08A61P1/02A61P31/04
Inventor 倪龙兴陶睿
Owner 倪龙兴
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