16 results about "Cariogenic bacteria" patented technology

The present invention aims to provide a novel and highly safe composition or agent for inhibiting the growth of normal oral bacteria, specifically a composition or agent for inhibiting the growth of cariogenic bacteria, periodontal disease bacteria, or Candida bacteria, as well as a composition or agent for preventing caries, preventing periodontal disease, and inhibiting biofilm formation, and a composition or agent for preventing aspiration pneumonia. [Solution] The inventors have discovered that heated ginger extract can effectively inhibit the growth of cariogenic bacteria, periodontal disease bacteria, or Candida bacteria, and therefore the above problem can be solved by preparing a composition or agent containing heated ginger extract as an active ingredient and using this as a composition or agent for oral use.

A fully dissolvable anticaries remineralization toothpaste for children and a preparation method thereof. The toothpaste has a three-layer composite structure: a dissolvable backing protective layer, an anticaries remineralization active layer, and a pH responsive layer. All components are food grade or pharmaceutical excipient grade, and completely dissolve within 10-30 minutes in the oral environment, without the need to remove after use. The active layer contains a "fluoride-nano hydroxyapatite-xylitol" ternary synergistic system, which can inhibit demineralization, promote remineralization, and inhibit cariogenic bacteria. The active layer also contains pH-responsive food colorants, which visually indicate the end of use through color changes. Formulas are designed for children aged 3-6 and 6-12, respectively, and the toothpaste can be cut along the preset line to fit different arch sizes. The invention solves the problems of poor use compliance, high risk of swallowing, and short residence time of active ingredients in existing children's anticaries products, and achieves safe, effective, and fun oral care for children.

The invention belongs to the technical field of microorganisms, and particularly relates to a composite probiotic postbiotic composition as well as a preparation method and application thereof. The composition disclosed by the invention comprises a saliva combined lactobacillus metagen, a lactobacillus paracasei metagen, a lactobacillus rhamnosus metagen and a lactobacillus acidophilus metagen. According to the invention, saliva is combined with the lactobacillus after-element, the lactobacillus paracasei after-element, the lactobacillus rhamnosus after-element and the lactobacillus acidophilus after-element, so that the anti-caries effect is achieved through multiple ways. On one hand, the composition of the composite probiotic metagen provided by the invention can be combined with cariogenic bacterium surface receptors, interference bacteria are adhered to tooth surfaces, and biofilm formation is reduced; and on the other hand, the organic acid in the composition of the composite probiotic metagen provided by the invention can reduce the pH value of the oral cavity and inhibit the growth of acid-producing bacteria, and meanwhile, certain protein and peptide components can enhance the hardness of enamel and improve the acid resistance of teeth.

The invention relates to the technical field of biological medicine, and particularly discloses application of Xinjiang pomegranate rind ellagic acid in caries prevention, in-vivo and in-vitro experiments prove that the ellagic acid can effectively inhibit floating growth and biofilm formation of streptococcus mutans, streptococcus sobrinus and streptococcus sanguis, MICs of the ellagic acid are 4 mmol / L, 2 mmol / L and 1 mmol / L respectively, and MBICs of the ellagic acid are 8 mmol / L, 4 mmol / L and 1 mmol / L respectively; meanwhile, the ellagic acid can obviously inhibit the acid production capacity of the cariogenic bacteria, the generation of water-insoluble exopolysaccharides and the adhesion to hydroxyapatite; in a rat caries model, by locally applying 1-8 mmol / L ellagic acid, the number of streptococcus in the oral cavity can be reduced in a dose-dependent manner, the caries damage degree of smooth surfaces of molar teeth and fossa and fissure can be relieved, the effect of the ellagic acid is equivalent to that of positive control drugs chlorhexidine and sodium fluoride under the high dose (8 mmol / L), and the ellagic acid shows good biological safety. Therefore, ellagic acid can be used for preparing safe and effective anti-caries drugs or oral care products.

The application provides a preparation method of a multifunctional toothpaste HA material, and under the premise of taking double-stage hydroxyapatite as a main efficacy component of the toothpaste, the toothpaste shows excellent properties in terms of anti-cariogenic bacteria performance, bacteriostatic performance and promotion of enamel remineralization, and under the condition of taking an ionic liquid+water as an electrolyte, the electrochemical preparation of the double-stage hydroxyapatite also has the characteristics of simple process, high cost performance, non-toxicity, no environmental pollution and convenience for industrialization.

The application discloses a hydrogel device and method for oral pH detection and sonodynamic therapy. The device comprises a hydrogel packaging layer and a pH sensing coupled sonodynamic therapy module; the hydrogel packaging layer is formed by two layers of network crosslinking, the first layer of network acts as a rigid but fragile skeleton, and the second layer of network is soft and stretchable, so that the hydrogel device has excellent mechanical properties and deformation ability; the pH sensing coupled sonodynamic therapy module is a wrapped structure, the outside is used for pH sensing, and reflects the caries damage condition of the oral cavity; the inside of the pH sensing coupled sonodynamic therapy module is a sonodynamic therapy module, which is used for generating active oxygen under ultrasonic stimulation, realizes the double effect of disinfection and tooth whitening on oral cariogenic bacteria, and the method provides an instant detection and nursing platform for oral health condition, and has the advantages of adhesion type, customizability, in-situ sensing and non-invasive treatment.

The invention relates to the technical field of biological medicines, particularly discloses application of Aspertaichunol and derivatives thereof as antibacterial agents, and further discloses an oral care composition which comprises the antibacterial agent. According to the invention, the application of the Aspertaichunol and the derivative thereof as the antibacterial agent in oral care is explored for the first time, the application range of the Aspertaichunol and the derivative thereof is greatly widened, and the antibacterial agent disclosed by the invention shows an accurate antibiotic-level antibacterial effect on cariogenic bacteria streptococcus mutans, can effectively block adhesion and colonization of the streptococcus mutans on the surface of teeth, and can be used for preparing oral care products. After being applied to oral care products, the compound can effectively prevent and treat dental caries, and can provide safer and more effective anti-caries choices for the market.

The purpose of the present invention is to establish a simple, efficient, and rapid genetic testing method for bacteria in clinical settings, and to provide: a gene amplification method for specifically detecting and evaluating the amount of bacteria associated with periodontal disease and caries; a primer set for a gene amplification reaction; a kit for detecting genes of bacteria that cause periodontal disease or caries, the kit including the primer set; and a kit for evaluating the severity of periodontal disease or caries. The present invention is a method for detecting oral bacteria that are present in plaque or saliva above or below the gingival margin, or bacteria that cause periodontal disease or caries, by detecting genes of the oral bacteria or causative bacteria using a gene amplification method, the detection method being characterized in that (1) annealing and extension reactions in the gene amplification method are implemented at substantially the same temperature, and / or (2) the gene length of a target gene as an amplification product is short.

The invention belongs to the technical field of dental restoration material preparation, and particularly relates to a betulinol-based epoxy monomer, composite resin thereof and a preparation method. According to the preparation method of the betulinol-based tricyclic epoxy monomer, the betulinol tricyclic oxidized monomer is prepared through esterification and epoxidation reaction, the reaction condition is mild, the product purity is high, the monomer is used for preparing a composite resin matrix through photocuring, the compatibility is excellent, and the betulinol-based tricyclic oxidized monomer is prepared. And the special three-dimensional structure can effectively reduce the volume shrinkage of the material during curing. Good flexural strength (greater than or equal to 100 MPa) of the composite resin is maintained, and meanwhile, the polymerization shrinkage rate is as low as about 1%; more prominently, the antibacterial activity of the betulinol enables the material to have a remarkable inhibition effect on common cariogenic bacteria (such as streptococcus mutans). The invention provides an innovative solution with low shrinkage characteristic and active antibacterial function for dental caries repair, and has important clinical value.

The invention belongs to the technical field of nano-drugs, and particularly relates to a preparation method of a Ti3C2 composite nano-material. The preparation method comprises the following steps: mixing a Ti3C2 suspension and an antibacterial agent solution, and stirring in a dark environment to obtain the Ti3C2 composite nano material. The invention further provides application of the Ti3C2 composite nano material in preparation of products for killing cariogenic bacteria and application of the Ti3C2 composite nano material in preparation of products for preventing and / or treating caries. The Ti3C2 composite nano-material prepared by the invention can effectively kill cariogenic bacteria under the photo-thermal action mediated by the Ti3C2 composite nano-material, so that the occurrence of caries is prevented, and the simple and effective photo-assisted antibacterial strategy is expected to provide a new way for preventive clinical intervention, and lays a foundation for solving the problem of early caries of children.

The invention relates to the technical field of biology, and discloses an antibacterial composite peptide PMF and a preparation method and application thereof, the antibacterial composite peptide PMF is formed by connecting an antibacterial functional domain FP4-1, a biological membrane targeting domain PGK and a cariogenic bacteria targeting domain CSPM8 through lysine residues; wherein the amino acid sequence of the antibacterial functional domain FP4-1 is IKNLVKKMIKLLY and is shown as SEQ ID NO.1, the amino acid sequence of the biological membrane targeting domain PGK is YGKQSKP and is shown as SEQ ID NO.2, and the amino acid sequence of the cariogenic bacterium targeting domain CSPM8 is TFFRLFNR and is shown as SEQ ID NO.3. The invention further discloses a preparation method of the antibacterial peptide. According to the present invention, the precise targeting property, the ecological safety and the efficient antibacterial activity can be concurrently provided.

This invention discloses a visual anti-caries probiotic lozenge and its preparation method, belonging to the field of pediatric oral care technology. The invention uses *Lactobacillus rhamnosus* SD11 as the core functional strain, employing spray drying technology for single-layer microcapsule encapsulation, combined with a pH-responsive natural pigment color development mechanism, and compounded with xylitol, calcium carbonate, and vitamin D3 to produce an oral lozenge. This product inhibits cariogenic bacteria by regulating the oral microecology, achieving caries prevention, and visually indicates oral health status through color changes. Simultaneously, the strain can reach the intestines to exert systemic regulatory effects. This invention utilizes spray drying microencapsulation and dry granulation processes, resulting in high production efficiency, stable quality, and advantages such as high safety, good compliance, clear efficacy, and suitability for industrial production. It is suitable for long-term daily use by children aged 2-12 years.

The invention belongs to the technical field of caries prevention, and provides an anti-caries material as well as a preparation method and application thereof. According to the anti-caries material, natural drugs tannic acid and berberine are self-assembled to form composite micelles, and amorphous calcium phosphate is adsorbed on the outer layer of the composite micelles. The anti-caries material disclosed by the invention can inhibit proliferation of cariogenic bacteria and formation of a biological membrane in an acidic microenvironment, and meanwhile, calcium and phosphorus ions are released to repair early caries by promoting remineralization. In conclusion, the anti-caries material provided by the invention has anti-caries principles of bacteriostasis, biofilm resistance and remineralization, overcomes the defect of single anti-caries principle of fluoride and a chemical bacteriostatic agent of the existing anti-caries material, and improves the anti-caries performance of the anti-caries material.

The invention provides a fermented clove preparation as well as a preparation method, a composition and application thereof, and relates to the technical field of oral health. According to the invention, the bioavailability of the clove is improved in a manner of fermenting the clove by lactobacillus plantarum, so that the specific biological activity of the clove is fully released, and the oral care effect of the clove is further improved. Besides, the fermented clove preparation is used for intervening decayed teeth, the problem that the efficacy is reduced due to the fact that viable bacteria are sensitive to environmental conditions is solved, and the validity period of the product is prolonged. Hyaluronic acid is also added to form the composition with the fermented clove preparation, so that the protection effect on the enamel is further enhanced. The hyaluronic acid has an anti-adhesion effect, can prevent the generation of a dental plaque biofilm, reduces the permeability of bacteria to tissues, and reduces the transmission rate of cariogenic bacteria. In addition, the hyaluronic acid can also promote implantation and survival of probiotics, further enhance the anti-decayed tooth effect, and realize long-acting anti-decayed tooth.

This invention provides a dendritic mesoporous silica and its preparation method that can inhibit key pathogenic processes such as growth, acid production, sugar production, and biofilm formation of cariogenic bacteria and achieve inhibitory effects on cariogenic bacteria in complex microbial communities. This invention does not aim to simply kill all bacteria, but rather utilizes the acidic microenvironment of caries to trigger drug release. By inhibiting key pathogenic processes such as growth, acid production, sugar production, and biofilm formation of cariogenic bacteria, it reduces the overall cariogenic toxicity of the biofilm. This "functional inhibition" strategy is theoretically more conducive to maintaining the stability of the microbial community structure while reducing the pathogenicity of the biofilm.

The invention discloses a curcumin-based carbon dot nano-enzyme cascade catalytic system and application thereof in targeted anti-cariogenic bacteria, and belongs to the technical field of biological medicine, the catalytic system is prepared by mixing curcumin-based carbon dot nano-enzyme Cur(at) Fe, Mn / CDs and glucose oxidase GOx, and performing ultrasonic treatment and incubation; the curcumin-based carbon dot nano-enzyme cascade catalytic system shows near-infrared enhanced peroxidase activity and excellent photo-thermal performance, hydrogen peroxide generated by GOx oxidized glucose is used as a substrate of peroxidase mimetic enzyme Cur(at) Fe, Mn / CDs, high-toxicity hydroxyl free radicals are generated in an acid environment, and the activity of the peroxidase mimetic enzyme Cur(at) Fe, Mn / CDs is enhanced. According to the present invention, the positive charges on the surface of the nano-enzyme and the GOx are utilized to accurately position the position of the biological membrane, such that the targeted bacteriostasis can be performed on the cariogenic bacteria of S.mutans and S.sanguinis while the normal oral cavity S.oranis is almost not affected, and the new path is provided for the caries treatment.