141 results about "Glucose production" patented technology

The present invention relates to glucose production, and is one whole membrane method of producing high purity crystalline glucose in relatively low cost. The method includes the following steps: liquefying starch to obtain starch liquid with DE value of 14-25 %; cooling and adding compound amylase to enzymolyze into glucose liquid; microfiltering to obtain dialysate and filter residue; drying the filter residue to produce feed; ultrafiltering the dialysate to obtain ultrafiltrate and ultrafiltered concentrated liquid; nanofiltering the ultrafiltrate to obtain nanofiltrate and nanofiltered concentrated liquid; concentrating the nanofiltrate to obtain concentrated glucose liquid; and spray drying to obtain crystalline glucose product. The nanofiltrate has DX value higher than 99.5 % and the method has no crystallization step and 10 % raised glucose yield. The method can recover starch and polysaccharide and is environment friendly.

The present invention pertains to a method of converting cellulose to glucose by treating a pretreated lignocellulosic substrate with an enzyme mixture comprising cellulase enzyme and endoglucanase core proteins, wherein the endoglucanase core proteins are present in the enzyme mixture at an amount relative to all endoglucanases from about 35 wt. % to about 100 wt. % and wherein the endoglucanase cellulase enzymes are present in the enzyme mixture at an amount relative to the amount of CBH and EG enzymes from about 2 wt. % to about 50 wt. %. The pretreated lignocellulosic substrate is selected from the group consisting of agricultural residues, residues after starch or sugar removal, dedicated ethanol crops, forestry products, and pulp and paper products, or combinations thereof.

The invention relates to a disposable multi-channel electrochemical immunosensor with high sensitivity. A prussian blue composite, nanogold and a capture antibody are assembled layer by layer on a disposable printing electrode array to obtain a multi-channel immunosensor. Enzyme and secondary antibody in great proportion are assembled on a carbon nano tube loading gold nanoparticles and a novel glucose oxidase functional nano composite probe is designed for sandwich immunoassay. The nano composite probe is combined with the multi-channel immunosensor to realize double signal amplification and high sensitive immunodetection of protein. The prussian blue, as electronic transmission media, catalyzes and reduces hydrogen peroxide generated by glucose oxidation under the catalysis of glucose oxidase on oxygen so as to obtain current signal. The method avoids the interference of dissolved oxygen in the detection solution and deoxidization is not required in process of amperometric detection. The invention has the advantages of wide detection concentration range, good repeatability, accurate results and the like and has a certain clinical application value.

An electronic system is provided that simulates a glucose-insulin metabolic system of a T2DM or prediabetic subject, wherein the system includes a subsystem that models dynamic glucose concentration in a T2DM or prediabetic subject, including an electronic module that models endogenous glucose production (EGP(t)), or meal glucose rate of appearance (Ra(t>>, or glucose utilization (U(t)), or renal excretion of glucose (B(t)), a subsystem that models dynamic insulin concentration in said T2DM or prediabetic subject, including an electronic module that models insulin secretion (S(t)), an electronic database containing a population of virtual T2DM or prediabetic subjects, each virtual subject having a plurality of metabolic parameters, and a processing module that calculates an effect of variation of at least one metabolic parameter value on the glucose insulin metabolic system of a virtual subject by inputting the plurality of metabolic parameter values.

In a method of determining insulin sensitivity in a patient, glucose level is sensed continuously. A first area under the curve representing the glucose level over time is calculated. An amount of insulin that has been administered to the patient is sensed. An estimation of insulin on board the patient is calculated based on the glucose level and the amount of insulin administered to the patient. A second area under the curve representing the insulin on board over time is calculated. Patient data indicative of at least one patient physical parameter is received. Information indicative of amount of glucose ingested by the patient during a meal is received. An insulin sensitivity output indicative of ability of insulin to stimulate glucose utilization and inhibit glucose production in the patient based on the first and second area under the curve, the patient data and the meal information is generated.

An application of the acicular hydroxyapatite nanoparticles in preparing toothpaste for repairing and strengthening tooth and preventing decayed tooth is disclosed.

The invention discloses a preparation method of RS5 wheat resistant starch. The preparation method comprises the following steps: firstly performing de-branching treatment on wheat starch through pullulanase to obtain de-branched wheat starch; then compounding the de-branched wheat starch and glycerin monostearate; finally improving the content of the wheat resistant starch through heat-moisture treatment. The content of the resistant starch in wheat starch is more than 70 percent. The wheat resistant starch prepared by the preparation method disclosed by the invention has the characteristicsof being stable in property, high in safety, blood glucose production index and the like, and easily realizes the industrial production.

The invention relates to a fluorescent enzyme-linked immunoassay method for detection of galectin-4. According to the method, glucose oxidase on an enzyme labeled complex combined through a target substance catalyzes glucose to generate hydrogen peroxide, then a ferrous ion solution is added to generate reactive oxygen for quenching fluorescence of a gold nanocluster solution, and fluorescence change is determined by a fluorescence spectrometer for quantitative detection of a target molecule. Fluorescent gold nanoclusters used in the method have stable luminescence and are sensitive to response to the reactive oxygen, and are more stable in controllability and more convenient to operate when used as a detection probe. The detection system constructed by the method has the advantages of simple synthesis, low cost, and high sensitivity and stability, and is expected to be more widely applied and extended in clinical biomolecular detection.

The object of the present invention is to provide a microorganism having an excellent ability to specifically oxidize the hydroxymethyl group of glucose, and a method of producing glucuronic acid and/or glucuronolactone by using such a microorganism to directly oxidize glucose, and the invention is directed at a microorganism which produces glucuronic acid directly from glucose and a mutant strain which is capable of specifically oxidizing the hydroxymethyl group of glucose and in which a DNA nucleotide sequence corresponding to 16S rRNA has the nucleotide sequence of SEQ ID NO: 1 in the sequence listing, and the invention is also directed at a method of producing glucuronic acid and/or glucuronolactone by specifically oxidizing the hydroxymethyl group of glucose using the mutant strain, and this invention enables D-glucuronic acid and/or D-glucuronolactone to be produced and furnished easily and safely at a high yield and a low cost.

A method for producing an ex vivo population of human insulin-producing cells that produce insulin in response to glucose from a patient is disclosed. The population of insulin producing cells is derived from human epithelial cells by causing those epithelial cells to differentiate into insulin producing cells. In one embodiment, the epithelial cells are derived from human mesenchymal stem cells (MSCs). The MSCs can be cultured from human bone marrow. In one embodiment, a culture of epithelial cell linage is generated by cultivating a sample of human cells containing a mixed culture containing human MSCs to produce an enriched culture of MSCs having a higher concentration of MSCs than the sample; and then differentiating cells from the enriched culture into cells of the epithelial cell lineage.

The invention relates to a preparation method of a self-energy-providing glucose inspection electrochemical sensor based on ZnxBi2S3+x sensitized NiTiO3. The method successfully enables ZnxBi2S3+x tobe modified in situ to NiTiO3 surface through a continuous ion layer adsorption mode, thereby making up for the problem of low utilization efficiency of NiTiO3 for visible light; and meanwhile, through a matched band-gap structure and excellent sensitization, photoelectric conversion efficiency is greatly improved, and good photoelectric response is obtained. Glucose oxidase is modified on an electrode and catalyzes glucose to be detected in an electrolyte solution to produce gluconic acid and hydrogen peroxide, wherein the latter serves as an electron donor and can be combined with photogenerated holes generated by the ZnxBi2S3+x, thereby well hindering combination of photo-induced electron-holes, further promoting transfer and transmission of photo-induced electrons and improving sensitivity of the optoelectronic signal and the prepared sensor; by catalyzing different concentrations of glucose, different degrees of photoelectric response is generated, and detection of glucose is realized; and limit of detection is 0. 35 muM.

The invention provides a method of producing high fructose corn syrup and glucose in a same production line. A certain amount of glucose syrup is added into a mother liquor so that the mixture can meet the requirements of monosaccharide components of F42 high fructose corn syrup; the mixture is subjected to isomerization so as to convert glucose into fructose after isomerization; and then discoloring, ion exchanging, concentrating, and packaging are performed to produce F42 fructose syrup. A conventional glucose production technology is optimized, a high concentration liquefying production method is adopted; high fructose corn syrup and glucose are co-produced; the byproducts of glucose are recovered and reutilized; investment, water resources, and energy are saved, and energy saving and emission reduction are realized.

The invention belongs to the technical field of glucose production and particularly relates to a high-purity and high-polymerization-degree glucose production method. The method comprises the steps of material mixing, polymerization, reaction ending, decoloration, membrane separation, ion exchange, concentration and spray drying. By the aid of the method, the production program is simplified greatly, material are not in contact with the outside, secondary pollution and cross pollution of human factors to finished products are prevented or reduced, the product quality is good, and the production cost is low.

The invention provides a method for improving the bagasse enzymolysis efficiency through delignification pretreatment and addition of an additive. The method comprises the steps of delignification pretreatment of a bagasse raw material, enzymolysis and the like. The air-dried and smashed bagasse raw material is mixed with a sodium hydroxide solution with a certain concentration or an acid sodium chlorite solution with a certain concentration, a reaction is conducted at the temperature of 20-180 DEG C for 0.5-12 hours for pretreatment, and pretreated residues rich in cellulose are obtained; cellulase and the additive are added into the residues for enzymolysis, so that the enzymolysis efficiency is improved. According to the method, through sodium hydroxide or sodium hypochlorite pretreatment and addition of the additive in the enzymolysis stage, the enzymolysis efficiency is improved, the enzymolysis time is shortened, the glucose production cost is reduced, and the method has a good application prospect.

Provided are a bacillus licheniformis high-temperature alpha-amylase mutant and application thereof.A nucleotide sequence of the high-temperature alpha-amylase mutant is shown as SEQ ID NO.1, and an amino acid sequence of the high-temperature alpha-amylase mutant is shown as SEQ ID NO.2.The mutant is obtained by conducting protein engineering improvement and selection on high-temperature alpha-amylase from bacillus licheniformis, the glucose content in a product of hydrolyzed starch can be improved and the dextrin and oligosaccharide content can be reduced by applying mutant enzyme in the hydrolyzed starch, the glucose content in syrup obtained through separate starch hydrolysis is 72%, the using amount of saccharifying enzyme in the follow-up further glucose production can be decreased, and reduction of the production costs is promoted.

The invention discloses a method for preparing isomalto-oligosaccharide from glucose BD liquor. The method comprises the following steps: (1) glucose BD liquor preparation,(2) decolorization and filtration, (3) ion exchange, (4) glucoside conversion, (5) enzyme deactivation, (6) filtration, (7) decolorization, (8) ion exchange and (9) steaming: a discharged material after ion exchange in the step (8) is pumped into a five-effect falling-film evaporator for evaporation and concentration until the mass percent concentration of the material is 70-75% to prepare isomalto-oligosaccharide, wherein the discharge temperature ranges from 80 DEG C to 85 DEG C. Compared with the prior art, the method has the benefits that waste liquor (BD liquor) after chromatographic separation of glucose mother liquor in a crystal glucose production process is taken as a raw material, so that local materials are used, wastes are turned into wealth, and the glucose mother liquor in the crystal glucose production process is comprehensively utilized by 100%; the effects of no pollution and zero emission are achieved; the added value of a product is increased.

The invention discloses a wastewater treatment method in starch glucose production. The wastewater treatment method comprises the following steps of: (1) carrying out flocculent precipitation, namely, adjusting the pH value of starch glucose production wastewater, and subsequently adding a flocculant to precipitate; (2) using a horizontal spiral centrifuge, namely, eliminating solid matters in flocculated wastewater by using the a horizontal spiral centrifuge so as to obtain deslagged wastewater; (3) carrying out anaerobic reaction, namely, carrying out anaerobic degradation reaction on the deslagged wastewater after being subjected to mixing buffering; (4) carrying out anoxia reaction, namely, carrying out an anoxia denitrification reaction on the wastewater subjected to anaerobic degradation; and (5) carrying out an aerobic reaction, namely, carrying out aerobic reaction on the wastewater subjected to anoxia denitrification reaction, carrying out ammonia nitrogen removal and further degradation. By utilizing the method, in the process that suspended matters are eliminated, the suspended matters or organic substances adhered to the suspended matters are effectively eliminated, the later organic substance treatment pressure is effectively alleviated, and the wastewater treatment method has the characteristics of low sewage treatment cost and good treatment effect.

The invention relates to a manufacturing method of disodium cytidine triphosphate, which comprises the following steps: proceeding enzymatic conversation with CMP material; proceeding ion exchange column chromatography; separating and purifying. The enzymatic conversation reaction principle is that: brewers' yeast gets the needed enzyme liquid of the enzymatic conversation reaction; brewers' yeast produces a plurality of biological enzyme as sugar glycolysis enzyme and phosphorylase with high sugar glycolysis ability; the biological energy produced by glycolysis glucose with sugar glycolysis enzyme is transferred to cytidine monophosphate by ATP; cytidine monophosphate and phosphate radical combines to form cytidine triphosphate with the effect of phosphorylase. The method shapes low cost, simple operation, high production, high product purity by optimization, which comprises the following steps: getting enzyme liquid; proceeding enzymatic conversation by adding the main material cytidine monophosphate, monobasic potassium phosphate of proper quantity and glucose; getting product by deposition, ion exchange column chromatography, segregation, purification, refining and vacuum drying.

The saccharizing process for glucose production includes the following steps: setting crystallized glucose mother liquid or the mixture of crystallized glucose mother liquid and starch emulsion into saccharifying tank; and saccharifying via adding enzyme of 0.4-0.5 L/T at pH 4.2-4.5 and 60-62 deg.c for 40-60 hr. The process has raised crystallized glucose mother liquid utilizing rate, disaccharide, trisaccharide and other oligosaccharides converted into glucose, DE value up to 91-96 %, raised glucose yield and low production cost.

The invention relates to a glucose production process, in particular to a glucose production process for reducing steam consumption. The invention aims at providing the glucose production process which fully utilizes the material temperature difference of the glucose processes to exchange material heat for reducing the steam consumption and saving energy and reducing comsumption. The technical scheme of the invention is as follows: the glucose production process for reducing steam consumption comprises: decoloration, ion exchange, and evaporation procedure; a heat exchanging system is arranged before decoloration, and through the heat exchanging system, the steam condensate is used for heating glucose solution. The benefits of the invention are as follows: the problem of high steam consumption in the current glucose production procedure is solved. The steam consumption for producing per ton of finished product of glucose is reduced by 0.28 t, and the production cost is reduced by 29.4 RMB.

A preparing method of a tumor catalytic nanometer reactor based on graphene quantum dots and application of the reactor are disclosed. The method includes (1) preparing graphene quantum dots (Py-GQDs)having peroxidase activity; and (2) activating, with carbodiimide/N-hydroxysuccinimide, carboxyl ends of the Py-GQDs prepared in the step (1) at room temperatures, and covalently linking the Py-GQDsprepared in the step (1) o amino ends of glucose oxidase (Gox) through amidation to prepare the tumor catalytic nanometer reactor PyG-Gox. The reactor is advantageous in that the prepared reactor, ina tumor slightly acid environment, firstly degrades glucose in the environment to generate a large amount of hydrogen peroxide and further degrades the hydrogen peroxide into hydroxyl free radicals, thus generating obvious killing effects for tumor cells, and therefore, the reactor can be applied for preparing antitumor medicines. In addition, the reactor is extremely low in catalytic efficiency in neutral conditions so that the reactor shows extremely good biocompatibility and does not poison normal tissues.

A crystallization process for glucose production features several horizontal crystallizers connected serially for continuous feeding, continuous crystallization and continuous discharging. The stable continuous operation of the crystallization process is maintained by controlling the material inlet temperature, discharging temperature and other several main parameters. Compared with the traditional crystallization process, the present invention has the advantages of low investment in equipment, high production efficiency, continuous stable operation of crystallization process, high product quality and high yield of crystallized glucose.