38 results about "Glucose production" patented technology

The invention relates to a lignocellulose biomass glucose production prediction method based on machine learning, and aims to improve the yield of glucose and optimize a biomass conversion process. According to the method, machine learning models such as a multi-layer perceptron, support vector regression, a random forest, a gradient boosting decision tree and a convolutional neural network are adopted to predict the LCB glucose yield processed by different preprocessing modes. Experimental results show that the convolutional neural network model is optimal in performance, the decision coefficient Rreaches up to 0.95, and the method has excellent generalization ability and prediction precision. The prediction precision is remarkably improved by feature extraction and principal component analysis in the model establishment process, and the Rof the convolutional neural network model is improved from 0.76 to 0.95. The efficient and accurate prediction method is provided for optimization of the biomass saccharification process, the method can be widely applied to the field of biological energy, and technical support is provided for improving the resource utilization efficiency of lignocellulose biomass.

The invention provides a trimetal nano enzyme as well as a preparation method and application thereof. The ABC trimetal nano-enzyme disclosed by the invention has multi-enzyme activities of glucose oxidase, peroxidase, catalase, glutathione peroxidase, nicotinamide adenine dinucleotide phosphate oxidase and the like, and can be used for enhancing the catalytic treatment on tumors. In a tumor microenvironment, the component A has glucose oxidase-like activity, and can catalyze endogenous glucose to generate hydrogen peroxide to enhance the generation of hydroxyl radicals; the component B has peroxidase-like and glutathione-like peroxidase activity, and can generate hydroxyl radicals and consume glutathione; the component C has peroxidase-like activity and catalase-like activity and can generate hydroxyl radicals, and generated oxygen can improve the glucose oxidase-like activity of the component A. In addition, the component BC has the activity of nicotinamide adenine dinucleotide phosphate oxidase, and oxygen can be catalyzed to generate superoxide anions by utilizing the excellent electron transmission characteristic of the interface of the component BC.

The invention provides an immunoassay method based on instant synthesis of a nano-enzyme of a bimetallic CuCo Prussian blue analogue (CuCo PBA), and belongs to the field of chemical and biological sensing. According to the method, a sandwich type immune mode is formed by a glucose oxidase labeled antibody, a target object and an immobilized antibody, so that the concentration of the target object is in direct proportion to the amount of glucose oxidase. Hydrogen peroxide generated by catalyzing glucose by glucose oxidase catalyzes 3, 3 ', 5, 5'-tetramethyl benzidine (TMB) to oxidize and develop color under the assistance of CuCo PBA nano-enzyme (Cu < 2 + > and K3 [Co (CN) 6]) which is generated instantly (Cu < 2 + > and K3 [Co (CN) 6] are added instantly). And the color change of the detection liquid is recorded and is used for visual detection and quantitative detection by a microplate reader. The problems of advanced synthesis and storage of nano materials are avoided, the cost is low, the colorimetric detection method is simple in operation process, rapid in color development and high in sensitivity, and a novel method which is convenient and stable in performance is provided for clinical immunological detection.

The invention discloses an alpha-1, 4 glucosidic bond hydrolytic ability enhanced oligomerized 1, 6-glucosidase mutant and application thereof, and belongs to the technical field of gene engineering and enzyme engineering. The oligomerized 1, 6-glucosidase mutant V219A / P227A disclosed by the invention also has the alpha-1, 4 glucosidic bond hydrolytic activity which is not possessed by a wild type enzyme on the basis of retaining the original alpha-1, 6 glucosidic bond hydrolytic activity, and the alpha-1, 4 glucosidic bond hydrolytic activity is improved by 267% compared with that of a single mutant V219A. The mutant V219A / P227A is applied to an enzymatic production process of glucose, maltodextrin is taken as a substrate, glucamylase and the mutant V219A / P227A are added for enzymatic conversion, and the final glucose yield reaches 98.0%; the mutant V219A / P227A is applied to regeneration of glucose from crystalline glucose mother liquor, and the final glucose yield reaches 95.9%, which is 9.35% and 5.15% higher than those of a wild type V219A and a single mutant V219A respectively. Therefore, the mutant is helpful for improving the substrate conversion rate and the product purity in the glucose production process, can be used for regenerating glucose from crystalline glucose mother liquor, and has higher industrial application value.

The utility model relates to the technical field of glucose production, and discloses a device for mixing calcium gluconate, which comprises a bracket assembly, a stirring box assembly is arranged at the top of the bracket assembly, an adjusting bracket assembly is arranged at the bottom of the bracket assembly, a stirring main body assembly is arranged in the stirring box assembly, and a stirring main body is arranged in the stirring main body assembly. And the bottom of the stirring main body assembly is provided with a convenient discharge storage box assembly. The convenient discharge storage box assembly is mounted on the device, so that after the stirring work of the device is completed, the convenient discharge work can be performed after the stirring work is concentrated in the convenient discharge storage box assembly, and the problem that the internal adhesion condition is unclear during discharge can be avoided through the assembly.

The application discloses an artificial intelligence-based wound pH detection method and a matched enzymatic in-situ forming hydrogel dressing and a preparation method, and belongs to the technical field of biomedical materials.The application identifies and analyzes a hydrogel image with a pH response reagent through a convolutional neural network algorithm, generates an optimal identification model to identify pH, and further evaluates a wound healing state, so that a new strategy is provided for monitoring and instant diagnosis.The matched enzymatic in-situ forming hydrogel dressing can be flexibly attached to various irregular wounds by consuming glucose through glucose oxidase to generate free radicals to initiate in-situ polymerization of the hydrogel.Meanwhile, the added quaternary amine chitosan has antibacterial function, and can better promote wound healing.The application can improve treatment efficiency and save treatment cost, and has the characteristics of safety, high efficiency and multifunction.

The present invention generally relates to implantable continuous blood glucose measurement technology. In particular, the present invention relates to an implantable continuous blood glucose measurement device for continuously measuring blood glucose in the body of a user, in which an oscillator for generating a variable frequency output signal corresponding to a voltage or current applied to both ends thereof is connected to a glucose fuel cell for generating electrical energy by glucose in the body, comprises an LED element, which is turned on or off by the output signal of the oscillator, or an antenna for wirelessly transmitting the output signal of the oscillator. The present invention has the advantage that blood glucose can be continuously measured in a state in which a blood glucose sensor is implanted in the body, without having to replace same.

The utility model relates to the technical field of cellulose glucose production equipment, in particular to cellulase pneumatic stirring and conveying equipment which comprises a tank body. An air inlet is formed in the lower portion of the tank body, a feeding port, a water inlet and an air outlet are formed in the upper portion of the tank body, and a discharging port is formed in the bottom of the tank body; the air inlet is arranged along the tangential direction of the tank body, the air inlet is connected with an air inlet pipeline, and the air outlet is connected with an air outlet pipeline; the feeder comprises a feeding funnel; the feeding funnel is connected with the feeding hole through a feeding pipeline; the discharge hole is connected with an emptying pipeline; a control valve is arranged on each pipeline; a spoiler is arranged in the tank body, and the flow of liquid is disturbed through the spoiler. The utility model has a simple structure, can avoid the problem of partial inactivation of cellulase caused by shearing force generated by matching the traditional mechanical stirring with a centrifugal pump when in use, effectively ensures the activity of cellulase, and plays a positive role in reducing the production cost and ensuring the product quality.

The present invention relates to a recombinant E. coli strain producing 1,3-butanediol from glucose and a method for producing 1,3-butanediol using same, in which a recombinant E. coli strain was developed in which a gene of a pathway necessary for biosynthesis of 1,3-BDO from acetyl-CoA is cloned, and a gene of a pathway that interferes with or competes with this pathway is removed. Additionally, an E. coli strain was developed in which the pathways and genes involved in the conversion of glucose to acetyl CoA, the regeneration rate of NADPH used as a cofactor, and the efficient use of the TCA cycle pathway, which are necessary to improve the biosynthetic efficiency of 1,3-BDO, are modified and optimized. Accordingly, the recombinant E. coli strain of the present invention can produce 1,3-butanediol from glucose at a high concentration, yield, and rate.

The inventive technology described herein includes systems, methods, and compositions for the upregulation of Uridine diphosphate glucose (UDPG) recycling in a yeast cell, by expressing a heterologous nucleotide sequence, operably linked to a promoter, encoding a phosphoglucomutase enzyme which catalyzes the conversion from glucose-6-phosphate to glucose- 1 -phosphate, and a UDP-glucose pyrophosphorylase enzyme which catalyzes the formation of UDPG from glucose 1 -phosphate and UTP. The increased levels of UDPG substrate produced by the yeast cell may be used as a substrate for a co-expressed UDP -glycosyltransferases enzyme capable of generating cannabinoid glycoside compounds.

The present invention provides a method for producing glucose, the method comprising bringing a liquid which contains cellulose and water into contact with a solid acid catalyst. The solid acid catalyst contains elemental carbon, elemental titanium, and silica, and has a sulfo group as a surface functional group. The present invention also provides a solid acid catalyst for glucose production, the solid acid catalyst containing elemental carbon, elemental titanium, and silica, while having a sulfo group as a surface functional group.

The invention relates to preparation and application of ROS (reactive oxygen species) responsive nanoparticles containing thioketal bonds, which can effectively solve the problems of poor biocompatibility, poor water solubility, uncontrollable release and the like of curcumin. The materials comprise curcumin, 4-carboxyphenylboronic acid, chitosan oligosaccharide and thioketal diacid. Chitosan oligosaccharide is modified by 4-carboxyphenylboronic acid, and then thioketal diacid is cross-linked to obtain the ROS responsive polymer. The polymer, curcumin and glucose oxidase are self-assembled in a solution to construct ROS-responsive nanoparticles. According to the nanoparticles prepared by the invention, due to the introduction of ROS-sensitive thioketal bonds and boric acid ester bonds, the controlled release of curcumin at a tumor part is realized under the stimulation of a tumor microenvironment. In addition, the encapsulated glucose oxidase can catalyze over-expressed glucose at tumor tissues to generate hydrogen peroxide, the ROS concentration of tumor cells is increased, the drug release efficiency is enhanced, and the treatment effect of the drug on breast cancer is effectively improved.

The present invention discloses an application of an iron-based probe responsive to active oxygen, which can disintegrate and form clusters after responding to hydrogen peroxide, and can enhance 1 H T2-weighted magnetic resonance imaging contrast effect, using liposome modification, through the high permeability and retention effect of solid tumors, the probe can passively target the lung cancer area, and the loaded glucose oxidase catalyzes glucose to produce hydrogen peroxide, thereby enhancing the contrast of the lung cancer area. 1 H T2-weighted magnetic resonance imaging effect. In addition, the probe can also induce ferroptosis in lung cancer cells. Combined with the loaded immune-activating peptide Tuftsin, the system can activate the local immune response of the tumor, and finally combine with the immune checkpoint blocker α-PD-L1 to enhance the immunotherapy effect of lung cancer.

The invention belongs to the field of molecular genetics, and provides a rice SS3a allele and application thereof in increasing the content of resistant starch in rice and reducing the glucose generation rate. The gene is deleted at the 2387th basic group G of the third exon of the rice SS3a gene and contains a gene sequence as shown in SEQ ID No. 1. The allele can significantly increase the amylose content in rice and change the amylopectin structure, so that the resistant starch content of rice is significantly increased, and the glucose generation rate of rice is reduced. Meanwhile, the allele can also significantly improve the gelatinization characteristic and the viscosity characteristic of the rice, and has small influence on the grain weight of the rice. The invention further relates to application of the new SS3a allele in rice breeding, the allele can be introduced through a cross breeding method, or the original SS3a gene is subjected to site-directed mutagenesis to form the allele, and the allele is used for cultivating a new high-resistance starch rice strain.

The present invention relates to compositions and methods for the production of UDP-sugars, including UDP-glucose and UDP-galactose. In particular, the present invention provides engineered cell lines for the production of UDP-sugars, and methods for the production of UDP-sugars using these engineered cell lines. Also provided herein are engineered cells for the production of UDP-glucose, wherein the engineered cells comprise one or more genetic modifications that increase UDP-glucose production by increasing the metabolic flux to the UDP-glucose precursor and / or by reducing carbon losses resulting from the production of by-products.

The utility model discloses a kind of anhydrous glucose production drying device, it includes: fixed shaft, collection tank and riser, the fixed shaft outside is spaced apart fixedly connected with multiple springs, multiple the spring one end fixedly connected with inner tube, the inner tube outer wall is spaced apart and is provided with multiple vibration motors, the both sides of the inner tube are provided with multiple first porous grooves, the collection tank inner wall is slidably connected with second filter screen, the collection tank is internally provided with non-woven fabric collection bag, the collection tank top is embeddedly connected with lid, the middle part of the lid is threadedly connected with bellows, the collection tank side wall is provided with first filter screen, the collection tank side wall is connected with suction pump. Through the above structure, material agglomeration can be prevented, quality problems caused by uneven drying can be reduced, and material residue can be reduced to improve yield.

The invention discloses a method for preparing glucose from starch, belongs to the technical field of preparing glucose by enzymolysis, and the method comprises the following steps: sizing, ultrasonic treatment, pretreatment, pre-expansion treatment, hydrolysis saccharification, filtration, desalination, concentration, and drying; the method for the pre-expansion treatment is to first add pre-treated gum arabic solution and carboxymethyl starch to the pre-treated starch slurry, stir for 20-30min and mix evenly at a speed of 600-800rpm, then warm up to 58-62°C, and keep 30-40min at this temperature to pre-expand starch particles. The method reduces the production energy consumption of glucose, shortens the production cycle, and improves the glucose yield and glucose value of glucose prepared by raw amylase, the glucose yield is 97.8-98.4%, and the glucose value is 96.2-96.6%.

The invention discloses an enzymatic cascade nanogel system targeting Lawsonia intracellularis and a preparation method of the enzymatic cascade nanogel system. The enzymatic cascade nanogel system comprises the following components: 0.05-2% of sodium alginate; 0.02 to 0.3 percent of calcium chloride; 2 to 6 percent of N, O-carboxymethyl chitosan; 0.2 to 1% of D-mannosamine hydrochloride; 0.05 to 0.2 percent of tilmicosin; 0.0005 to 0.002 percent of cerium dioxide nano enzyme; and 0.0005 to 0.002 percent of glucose oxidase. The enzymatic cascade nanogel system provided by the invention has a dual gel structure of a dynamic covalent bond core and an ionic bond shell, and can realize targeted programmed release of tilmicosin; the endocytosis of host cells on the enzymatic cascade nanogel system is actively enhanced, the intracellular transport capacity of tilmicosin is improved, and the intracellular drug concentration is improved; glucose oxidase consumes glucose to generate gluconic acid and H2O2 so as to realize'hunger therapy 'on Lawsonia intracellularis; h2O2 generated by decomposing glucose oxidase by cerium dioxide nano-enzyme generates high-concentration oxygen at an infected part, so that the living environment of the intracellular Lawsonia is deteriorated.

The present invention discloses a method for predicting the glycemic index of rice, and belongs to the technical field of food detection. The present invention develops an in vitro digestion model dedicated to rice, thereby obtaining in vitro digestion test parameters, using rice with known GI as a standard reference, establishing a mathematical function association between the in vivo GI index and the in vitro simulated digestion parameters, and realizing accurate prediction of the GI index of the rice to be tested, and its linear correlation r is above 0.95, and the prediction accuracy is high. The present invention reduces unreasonable or unnecessary steps in the traditional method of in vitro determination of the glycemic index of rice, and the operation is simpler. The present invention can also be applied in rice variety screening and rice breeding. At present, there is no unified method dedicated to predicting the in vitro rapid detection of rice GI value, which is original.

The utility model discloses a recovery device for industrial glucose production, which comprises a box body, supporting mechanisms are arranged at four corners of the lower end face of the box body, grooves are formed in the tops of the left end face and the right end face of the box body, extension seats are arranged in the two grooves in a sliding manner, and blanking grooves are formed in the two extension seats. Fixing plates are arranged on one sides of the opposite surfaces of the two extension seats, guide rods are symmetrically arranged on the two fixing plates, guide holes corresponding to the guide rods are formed in the left end and the right end of the box body, a discharging groove is formed in the bottom of the left end face of the box body, and a sliding groove is formed in the inner wall of the top end of the discharging groove; a sliding groove is formed in the left end face of the box body, a box door is slidably arranged in the sliding groove, an inclined base is arranged at the bottom end in the box body, a discharging base is arranged at the bottom of the left end face of the box body, the discharging base is obliquely arranged, and through the design of an extension base, different external workbenches can be conveniently recycled.

The present invention relates to a genetically engineered bacterium comprising a deletion of the ndh gene; deletion of one or more nuo genes; the one or more nuo genes are selected from the group consisting of: nuoA, nuoB, nuoC, nuoD, nuoE, nuoF, nuoG, nuoH, nuoI, nuoJ, nuoK, nuoL, nuoM, and nuoN; the one or more nuo genes are selected from the group consisting of: nuoA, nuoB, nuoC, nuoD, nuoE, nuoF, nuoG, nuoH, nuoI, nuoJ, nuoK, nuoL, nuoM, and nuoN; wherein the genetically engineered bacterium is capable of oxygen uptake, i.e. The genetically engineered bacterium is capable of using oxygen as an electron acceptor, and wherein the bacterium is genetically engineered to produce a fermentation product in the presence of oxygen. In particular, the present invention provides bacteria genetically engineered to produce lactic acid from glycerol or from glucose in the presence of oxygen. The invention also provides bacteria genetically engineered to produce isobutanol and / or ethanol from glycerol in the presence of oxygen. Methods of producing fermentation products using the genetically engineered bacteria of the present disclosure are also described.

The present invention introduces a heterologous formate-tetrahydrofolate ligase gene into a microorganism having a D-pantoic acid synthetic metabolic pathway, and assimilates one molecule of formate to generate one molecule of 5,10-methylenetetrahydrofolate, and uses the microorganism to produce a new process of ketopantoate and / or D-pantoic acid from formate and glucose.

The utility model provides a glucose continuous production device which comprises a reaction tank, a material storage tank arranged on the reaction tank, an acid liquid tank arranged on the reaction tank and a pH adjusting assembly arranged among the reaction tank, the material storage tank and the acid liquid tank, the pH adjusting assembly comprises a first metering pump located between the reaction tank and the material storage tank, and a second metering pump located between the reaction tank and the material storage tank. The first metering pump is located between the reaction tank and the acid liquid tank, the second metering pump is located between the reaction tank and the acid liquid tank, the pH tester is arranged on the reaction tank, the reaction degree in the reaction tank is reflected in real time through the pH tester, the flow of the material liquid and the flow of the acid liquid conveyed into the reaction tank are adjusted through the first metering pump and the second metering pump respectively, and manual control of an operator is not needed; an operator does not need to judge the reaction endpoint in the reaction tank by naked eyes through iodine test paper, personal errors are reduced, purity fluctuation of finished products is reduced, product quality is improved, and the device has the advantages of high automation degree, efficient and simple operation, low implementation cost and convenience in popularization and implementation.

The invention provides preparation of an H2O2 response type compound enzyme preparation and application of the H2O2 response type compound enzyme preparation in pre-preparing minced fillet products. The compound enzyme preparation is prepared from maltodextrin, glucose, a rosemary extract, sulfonated moringa seed shell polyphenol, glucose oxidase and moringa peroxidase through low-temperature mixing and spray drying. The preparation is added into minced fillet, and the minced fillet is subjected to ultrahigh pressure treatment for primary physical sterilization; h2O2 generated by high-pressure stress microorganisms activates glucose oxidase and catalyzes glucose to generate a large amount of H2O2 to realize signal amplification, so that moringa oleifera peroxidase is activated to catalyze polymerization of sulfonated moringa oleifera seed shell polyphenols, and a potent bactericide is generated in situ for secondary chemical sterilization; and the residual trace H2O2 can trigger the cascade reaction again to provide third long-acting protection, so that the texture, flavor and nutrition of the surimi are effectively maintained, and the shelf life is remarkably prolonged.

To provide a method enabling production of glucose from cellulose with high yield by utilizing a hydrolysis reaction of cellulose using a solid acid catalyst, and also to provide a solid acid catalyst for glucose production.SOLUTION: A method for producing glucose is provided, comprising bringing liquid including cellulose and water into contact with a solid acid catalyst. The solid acid catalyst includes carbon element, titanium element, and silica, and has a sulfo group as a surface functional group. The present invention also provides A solid acid catalyst for glucose production is also provided, the solid acid catalyst includes carbon element, titanium element, and silica, and has a sulfo group as a surface functional group.SELECTED DRAWING: None

The invention provides a glucose production method using diatomite to replace activated carbon, and relates to the technical field of glucose production, the production steps comprise: size mixing, liquefaction, saccharification, deslagging filtration, primary decoloration and filtration, primary evaporation, secondary decoloration and filtration, ion exchange, secondary evaporation, crystallization, separation and drying to obtain the finished product glucose. According to the invention, the diatomite and the active carbon are mixed, so that the efficiency of the decolorizing step is accelerated, the filtering time is shortened, the filtering quality is improved and is the same as that of pure active carbon, and the price of the diatomite is lower than that of the active carbon, so that the production cost is reduced, and the used mixed decolorizing agent is recycled again; the total utilization rate of the mixed decolorizing agent can be improved, the filtering capacity of the original decolorizing agent is improved to 80-90% from 60-70%, and meanwhile the yield and quality after production are improved.

The invention relates to the technical field of biology, in particular to ADAD2 mutant protein with a hypoglycemic function and application, the ADAD2 mutant protein is obtained by mutating the 235th site of ADAD2 protein from Gly to Arg, and the amino acid sequence is shown as SEQ ID NO.2. In various hepatocytes, ADAD2 is over-expressed, the gluconeogenesis process is promoted, and the glucose production rate is increased; the glycosylation process of the over-expression ADAD2 G235R mutant is inhibited; after the expression of the wild ADAD2 is up-regulated, the fasting blood glucose level of a mouse is increased, the glucose tolerance is impaired, and meanwhile, the gluconeogenesis ability is enhanced; after the ADAD2 G213R mutant is up-regulated, the fasting blood glucose level of a mouse is reduced, the glucose tolerance is impaired and the gluconeogenesis ability is also reduced, and the ADAD2 gene missense mutant and the potential treatment effect thereof in regulating blood glucose and preventing and treating abnormal glucose metabolism can be used for preparing medicines for treating hyperglycemia and metabolic syndromes.

This disclosure relates to mRNA therapy for the treatment of glycogen storage disease type 1a, (GSD-Ia), and related symptoms such as hypoglycemia. mRNAs for use in the invention, when administered in vivo, encode human glucose-6-phosphatase (G6Pase or G6PC), and functional fragments and variants thereof mRNAs of the invention are preferably encapsulated in lipid nanoparticles (LNPs) to effect efficient delivery to cells and / or tissues in subjects, when administered thereto. mRNA therapies of the invention increase and / or restore deficient levels of G6PC expression and / or activity in subjects. mRNA therapies of the invention further increase the glucose production, and reduce the abnormal accumulation of glycogen and / or glucose-6-phosphate associated with GSD-Ia.

The invention belongs to the technical field of photoresponse antibacterial dressings, and discloses PCN-coated Au / GOx-F68 / F127 antibacterial temperature-sensitive hydrogel and a preparation method and application thereof.The PCN-coated Au / GOx-F68 / F127 antibacterial temperature-sensitive hydrogel can complete conversion of gel and sol under the condition of 4-37 DEG C, PCN-coated Au nano-enzyme is constructed by means of an in-situ growth method, and after the PCN-coated Au nano-enzyme is integrated with GOx, a self-activated enzymatic cascade reaction is formed by means of the hydrogel. GOx decomposes glucose to generate H2O2 and gluconic acid, the pH of a wound is reduced, PCN-coated Au decomposes endogenous H2O2 under an acidic condition to generate ROS for sterilization, limitation of a high-blood-sugar and high-pH environment on catalytic activity is avoided, meanwhile, photodynamic synergistic antibiosis is achieved, the synthesis condition is mild, the process is simple, and economical and feasible effects are achieved. The hydrogel has thermosensitivity, can relieve wound pain, does not loosen after absorbing exudate, has no residue, and does not adhere to a wound surface when being removed. Under the irradiation of visible light, the methicillin-resistant staphylococcus aureus and the escherichia coli capable of producing extended-spectrum beta-lactamase can reach gt within 0-30 minutes; the clearance rate is 99.9%, and a new way is provided for developing novel medical dressings for treating diabetic bacterial infection wounds.

The utility model provides a kind of high-efficiency evaporation device for glucose production and processing, including the bottom bracket in the lowermost, evaporator is installed in the upper side of bottom bracket by inclined support frame, reflux filter box is installed in the side of evaporator by positive support frame;Discharge pipe is installed at the lowest position of evaporator, there is a gas outlet cylinder above the highest position of evaporator, there is a feeding port connected with the inside of evaporator in the side of gas outlet cylinder;Multiple condensing pipes are equipped in the inside of reflux filter box, shunt cylinder is connected in the one end of condensing pipe, the outer end of shunt cylinder is connected with the gas outlet cylinder in evaporator by connecting pipe.The utility model can guide the steam containing saccharide aqueous solution generated in evaporator into reflux filter box, steam is condensed into liquid state by condensing pipe, participate in crystallization by refluxing into evaporator, steam containing saccharide aqueous solution can be recycled, and the waste of raw material is reduced.