12 results about "Glucose production" patented technology

The application discloses an artificial intelligence-based wound pH detection method and a matched enzymatic in-situ forming hydrogel dressing and a preparation method, and belongs to the technical field of biomedical materials.The application identifies and analyzes a hydrogel image with a pH response reagent through a convolutional neural network algorithm, generates an optimal identification model to identify pH, and further evaluates a wound healing state, so that a new strategy is provided for monitoring and instant diagnosis.The matched enzymatic in-situ forming hydrogel dressing can be flexibly attached to various irregular wounds by consuming glucose through glucose oxidase to generate free radicals to initiate in-situ polymerization of the hydrogel.Meanwhile, the added quaternary amine chitosan has antibacterial function, and can better promote wound healing.The application can improve treatment efficiency and save treatment cost, and has the characteristics of safety, high efficiency and multifunction.

The inventive technology described herein includes systems, methods, and compositions for the upregulation of Uridine diphosphate glucose (UDPG) recycling in a yeast cell, by expressing a heterologous nucleotide sequence, operably linked to a promoter, encoding a phosphoglucomutase enzyme which catalyzes the conversion from glucose-6-phosphate to glucose- 1 -phosphate, and a UDP-glucose pyrophosphorylase enzyme which catalyzes the formation of UDPG from glucose 1 -phosphate and UTP. The increased levels of UDPG substrate produced by the yeast cell may be used as a substrate for a co-expressed UDP -glycosyltransferases enzyme capable of generating cannabinoid glycoside compounds.

The invention belongs to the field of molecular genetics, and provides a rice SS3a allele and application thereof in increasing the content of resistant starch in rice and reducing the glucose generation rate. The gene is deleted at the 2387th basic group G of the third exon of the rice SS3a gene and contains a gene sequence as shown in SEQ ID No. 1. The allele can significantly increase the amylose content in rice and change the amylopectin structure, so that the resistant starch content of rice is significantly increased, and the glucose generation rate of rice is reduced. Meanwhile, the allele can also significantly improve the gelatinization characteristic and the viscosity characteristic of the rice, and has small influence on the grain weight of the rice. The invention further relates to application of the new SS3a allele in rice breeding, the allele can be introduced through a cross breeding method, or the original SS3a gene is subjected to site-directed mutagenesis to form the allele, and the allele is used for cultivating a new high-resistance starch rice strain.

The invention discloses an enzymatic cascade nanogel system targeting Lawsonia intracellularis and a preparation method of the enzymatic cascade nanogel system. The enzymatic cascade nanogel system comprises the following components: 0.05-2% of sodium alginate; 0.02 to 0.3 percent of calcium chloride; 2 to 6 percent of N, O-carboxymethyl chitosan; 0.2 to 1% of D-mannosamine hydrochloride; 0.05 to 0.2 percent of tilmicosin; 0.0005 to 0.002 percent of cerium dioxide nano enzyme; and 0.0005 to 0.002 percent of glucose oxidase. The enzymatic cascade nanogel system provided by the invention has a dual gel structure of a dynamic covalent bond core and an ionic bond shell, and can realize targeted programmed release of tilmicosin; the endocytosis of host cells on the enzymatic cascade nanogel system is actively enhanced, the intracellular transport capacity of tilmicosin is improved, and the intracellular drug concentration is improved; glucose oxidase consumes glucose to generate gluconic acid and H2O2 so as to realize'hunger therapy 'on Lawsonia intracellularis; h2O2 generated by decomposing glucose oxidase by cerium dioxide nano-enzyme generates high-concentration oxygen at an infected part, so that the living environment of the intracellular Lawsonia is deteriorated.

The present invention relates to a genetically engineered bacterium comprising a deletion of the ndh gene; deletion of one or more nuo genes; the one or more nuo genes are selected from the group consisting of: nuoA, nuoB, nuoC, nuoD, nuoE, nuoF, nuoG, nuoH, nuoI, nuoJ, nuoK, nuoL, nuoM, and nuoN; the one or more nuo genes are selected from the group consisting of: nuoA, nuoB, nuoC, nuoD, nuoE, nuoF, nuoG, nuoH, nuoI, nuoJ, nuoK, nuoL, nuoM, and nuoN; wherein the genetically engineered bacterium is capable of oxygen uptake, i.e. The genetically engineered bacterium is capable of using oxygen as an electron acceptor, and wherein the bacterium is genetically engineered to produce a fermentation product in the presence of oxygen. In particular, the present invention provides bacteria genetically engineered to produce lactic acid from glycerol or from glucose in the presence of oxygen. The invention also provides bacteria genetically engineered to produce isobutanol and / or ethanol from glycerol in the presence of oxygen. Methods of producing fermentation products using the genetically engineered bacteria of the present disclosure are also described.

The present invention introduces a heterologous formate-tetrahydrofolate ligase gene into a microorganism having a D-pantoic acid synthetic metabolic pathway, and assimilates one molecule of formate to generate one molecule of 5,10-methylenetetrahydrofolate, and uses the microorganism to produce a new process of ketopantoate and / or D-pantoic acid from formate and glucose.

The invention relates to the technical field of biology, in particular to ADAD2 mutant protein with a hypoglycemic function and application, the ADAD2 mutant protein is obtained by mutating the 235th site of ADAD2 protein from Gly to Arg, and the amino acid sequence is shown as SEQ ID NO.2. In various hepatocytes, ADAD2 is over-expressed, the gluconeogenesis process is promoted, and the glucose production rate is increased; the glycosylation process of the over-expression ADAD2 G235R mutant is inhibited; after the expression of the wild ADAD2 is up-regulated, the fasting blood glucose level of a mouse is increased, the glucose tolerance is impaired, and meanwhile, the gluconeogenesis ability is enhanced; after the ADAD2 G213R mutant is up-regulated, the fasting blood glucose level of a mouse is reduced, the glucose tolerance is impaired and the gluconeogenesis ability is also reduced, and the ADAD2 gene missense mutant and the potential treatment effect thereof in regulating blood glucose and preventing and treating abnormal glucose metabolism can be used for preparing medicines for treating hyperglycemia and metabolic syndromes.

The invention provides a lactobacillus reuteri high-density fermentation method and application thereof, and belongs to the technical field of microbial fermentation. According to the fermentation method provided by the invention, the heme, the vitamin K compound and the inducer are exogenously added, so that the problem of active oxygen toxicity associated with aerobic respiration is effectively reduced, carbon metabolic repression generated by glucose is avoided, and the utilization efficiency of sucrose in the fermentation process is remarkably improved; and the high-speed proliferation of the thalli is ensured, and meanwhile, the extremely high cell activity is kept. Experimental data show that the viable count of the fermentation liquor can break through 1 * 10 < 11 > CFU / mL only in 8-9 hours, and the viable count of a final product can be larger than or equal to 1 * 10 < 13 > CFU / mL within 10 hours. According to the technology, the seed propagation cost and the time cost are greatly reduced, the obtained thalli are high in mechanical strength and stress resistance, and a creative solution is provided for efficient industrial production of lactobacillus reuteri.

The utility model is applicable to the technical field of food processing equipment, and provides a closed powdered sugar automatic reworking device which comprises a sugar dissolving tank body, a stirring structure is arranged in the sugar dissolving tank body, and the sugar dissolving tank body is connected with a heating structure, is further connected with a cyclone dust collector, a drying tower and a swinging screen forwards and is connected with a decolorizing tank backwards; the heating structure comprises a hot water pipe extending into the sugar dissolving tank body, and the hot water pipe is connected with a water heater; the stirring structure comprises a stirring rod arranged in the sugar dissolving tank body, and a plurality of groups of stirring paddles are arranged on the stirring rod. In conclusion, the device is relatively simple in structure, and fine micro powder and oversize powder can be collected and dissolved at the same time in the glucose production process, so that relatively good dissolution is realized; sealing treatment is realized in the whole reworking process, so that fine micro powder and oversize powder can be dissolved and mixed in a sealed environment under the condition of no pollution risk, the purity control is realized, and the standard and the requirement of glucose production are met; the whole reworking process does not need manual operation, the operation process is simplified, the labor cost is reduced, and the working efficiency is improved.

The invention discloses a curcumin-based carbon dot nano-enzyme cascade catalytic system and application thereof in targeted anti-cariogenic bacteria, and belongs to the technical field of biological medicine, the catalytic system is prepared by mixing curcumin-based carbon dot nano-enzyme Cur(at) Fe, Mn / CDs and glucose oxidase GOx, and performing ultrasonic treatment and incubation; the curcumin-based carbon dot nano-enzyme cascade catalytic system shows near-infrared enhanced peroxidase activity and excellent photo-thermal performance, hydrogen peroxide generated by GOx oxidized glucose is used as a substrate of peroxidase mimetic enzyme Cur(at) Fe, Mn / CDs, high-toxicity hydroxyl free radicals are generated in an acid environment, and the activity of the peroxidase mimetic enzyme Cur(at) Fe, Mn / CDs is enhanced. According to the present invention, the positive charges on the surface of the nano-enzyme and the GOx are utilized to accurately position the position of the biological membrane, such that the targeted bacteriostasis can be performed on the cariogenic bacteria of S.mutans and S.sanguinis while the normal oral cavity S.oranis is almost not affected, and the new path is provided for the caries treatment.

The invention provides a sugar control method for balancing lactic acid and complete sugar chain antibody production and application thereof, and the sugar control method comprises the following steps: inoculating CHO1 cells to a production medium, and carrying out first-stage culture D0-D7 and second-stage culture D8-11 in sequence; by controlling glucose deletion time in the second-stage culture process, lactic acid consumption in a cell culture system is promoted, and antibody glycosylation modification deletion is avoided; a control strategy is selected from any one of the following: D8-11 days of single sugar deficiency each day: the residual sugar concentration is maintained at 0 g / L, and the duration time is 3-5 hours; d8-11 days of intermittent sugar deficiency every day: maintaining the residual sugar concentration at 0 g / L for 3-5 hours, supplementing glucose consumption for 2-14 hours, and then maintaining the residual sugar concentration at 0 g / L for 3-5 hours; d8-11 days are lack of sugar once every day, GlcNAc and Mannose are supplemented, the concentration of residual sugar is maintained to be 0 g / L, and the duration time is 5-12 h.

The present invention can: efficiently and readily produce glucose from microalgae that accumulate starch in their cells; and obtain ethanol. During a preparation step of the glucose production method, microalgae are prepared on which a saccharifying enzyme acts on starch accumulated inside the microalgae cells, without disrupting the cell walls. In a saccharification step, starch inside the cells is saccharified and glucose is generated, by adding a saccharifying enzyme to the microalgae without a disruption treatment. The ethanol production method has a step in which, after the saccharification step, the glucose undergoes alcoholic fermentation and ethanol is generated.