A preparing method of a tumor catalytic nanometer reactor based on graphene quantum dots and application of the reactor

A graphene quantum dot, nanoreactor technology, applied in chemical instruments and methods, nanotechnology for materials and surface science, nanotechnology, etc., can solve problems such as the application of catalysis treatment of tumors that have not been seen, and achieve no toxic effect. , good biocompatibility

Active Publication Date: 2018-12-14
WENZHOU MEDICAL UNIV
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Problems solved by technology

[0003] At present, there are no research reports on the application of GQDs at ho...

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  • A preparing method of a tumor catalytic nanometer reactor based on graphene quantum dots and application of the reactor
  • A preparing method of a tumor catalytic nanometer reactor based on graphene quantum dots and application of the reactor
  • A preparing method of a tumor catalytic nanometer reactor based on graphene quantum dots and application of the reactor

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Embodiment Construction

[0027] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings.

[0028] Preparation Examples:

[0029] This embodiment includes the following steps:

[0030] (1) Using pyrene as a raw material, react pyrene with nitric acid at 80-100°C (preferably 90°C) to prepare a precursor: trinitropyrene, and freeze-dry it; Resuspension, after ultrasonic reaction, the mixture was added to the reaction kettle, graphene quantum dots (Py-GQDs) were prepared by hydrothermal reaction, and Py-GQDs were dialyzed by ionized water in the dialysis bag and freeze-dried, the molecular weight cut-off of the dialysis bag ≤3500, the condition of hydrothermal reaction is 180-200°C (preferably 190°C), 10-12 hours (preferably 11 hours).

[0031] (2) Carbodiimide / N-hydroxysuccinimide was used to activate the carboxyl terminus of the Py-GQDs prepared in step...

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Abstract

A preparing method of a tumor catalytic nanometer reactor based on graphene quantum dots and application of the reactor are disclosed. The method includes (1) preparing graphene quantum dots (Py-GQDs)having peroxidase activity; and (2) activating, with carbodiimide/N-hydroxysuccinimide, carboxyl ends of the Py-GQDs prepared in the step (1) at room temperatures, and covalently linking the Py-GQDsprepared in the step (1) o amino ends of glucose oxidase (Gox) through amidation to prepare the tumor catalytic nanometer reactor PyG-Gox. The reactor is advantageous in that the prepared reactor, ina tumor slightly acid environment, firstly degrades glucose in the environment to generate a large amount of hydrogen peroxide and further degrades the hydrogen peroxide into hydroxyl free radicals, thus generating obvious killing effects for tumor cells, and therefore, the reactor can be applied for preparing antitumor medicines. In addition, the reactor is extremely low in catalytic efficiency in neutral conditions so that the reactor shows extremely good biocompatibility and does not poison normal tissues.

Description

technical field [0001] The invention relates to a graphene quantum dot, in particular to a preparation method and application of a graphene quantum dot-based tumor catalytic nanoreactor that can produce an obvious killing effect on tumor cells. Background technique [0002] Graphene quantum dots (GQDs) have a large specific surface area, abundant and active edge functional groups, and stable autofluorescence, so they are widely used in drug delivery, biosensing, imaging and other fields. In addition, the study found that GQDs have a peroxidase activity similar to that of horseradish peroxidase, which can degrade hydrogen peroxide into hydroxyl radicals under acidic conditions. Therefore, GQDs are often used in the preparation of biosensors for glucose detection. [0003] At present, there are no research reports on the application of GQDs at home and abroad, but there is no application related to the catalytic therapy of tumors. Contents of the invention [0004] The obj...

Claims

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Application Information

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IPC IPC(8): C09K11/65C09K11/02B82Y20/00B82Y30/00B82Y40/00A61K33/44A61P35/00
CPCA61K33/44A61P35/00B82Y20/00B82Y30/00B82Y40/00C09K11/025C09K11/65
Inventor 齐蕾戴黎明
Owner WENZHOU MEDICAL UNIV
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