19 results about "High fructose" patented technology

The application provides a method for immobilizing glucose isomerase, wherein a bacterial suspension of glucose isomerase producing bacteria TTGI is added into a gelatin aqueous solution with a mass concentration of 60-150 g / L, so that the final concentration of the bacterial bodies in the system is 50-150 g / L, and a gel block is formed after the obtained mixed solution is solidified; the gel block is soaked in n-butanol for 10-30 min, then is taken out and soaked in a glutaraldehyde solution with a mass concentration of 5-25 g / L for 30-60 min, and then is taken out and washed with deionized water until neutral, so that immobilized glucose isomerase is obtained; the method has the advantages of wide material source, low price and simple immobilization technology; when the immobilized glucose isomerase is used to produce high fructose syrup, the conversion rate can reach 63.9%, and the requirement of directly producing HFCS-55 can be met; and the immobilized enzyme can still maintain more than 93% of the initial enzyme activity after being continuously used at high temperature for 30 batches.

This invention provides a crystallization tank for fructose production, belonging to the field of fructose production technology. It includes a tank body with two parallel vertical water distribution tanks fixed to one side. Connecting pipes are fixed to the outer sides of each water distribution tank, and multiple water distribution pipes extending into the tank body's internal interlayer are fixed to the inner sides of each tank. The internal interlayer of the tank body is divided into multiple rings of cooling chambers from top to bottom, each ring communicating with a corresponding water distribution pipe. An annular connecting cavity is opened at the top of the tank body, communicating with the topmost cooling cavity. A motor is fixed to the top of the tank body, with its shaft extending into the tank body and a vertical stirring shaft fixed thereon. A U-shaped stirring tube adapted to the tank's internal cavity is fixed to the bottom of the stirring shaft, and the top of the U-shaped stirring tube communicates with the connecting cavity. This invention employs multi-ring uniform cooling and simultaneous cooling with stirring, effectively improving the uniformity of cooling and thus increasing the fructose crystallization efficiency, resulting in good performance.

This application relates to the field of biotechnology, and more particularly to an immobilized enzyme for the synthesis of D-allulose and its application. The immobilized enzyme for the synthesis of D-allulose provided in this application has an epimerase in which at least one key site in the amino acid sequence shown in SEQ ID NO: 20 is mutated. This mutation is beneficial to improving the catalytic activity of D-fructose C3 epimerization to form D-allulose. Furthermore, the epimerase is immobilized on a resin support to form a highly stable immobilized enzyme. Through the above method, the immobilized enzyme exhibits excellent catalytic activity for the D-fructose C3 epimerization to form D-allulose, and can efficiently convert D-fructose to D-allulose, thus improving the efficiency of D-allulose synthesis.

This invention belongs to the field of pharmaceutical technology, specifically relating to the use of a 4-(4-diethylamino-1-methylbutanamino)-7-chloroquinoline derivative or its pharmaceutically acceptable salt, or its tautomer, meso compound, racemic compound, enantiomer, diastereomer, or pharmaceutical composition thereof as an active ingredient in the preparation of drugs for the prevention or treatment of obesity and obesity-related metabolic diseases. The 4-(4-diethylamino-1-methylbutanamino)-7-chloroquinoline derivative of this invention has significant therapeutic effects on obesity and related metabolic diseases. In obese mice induced by a high-fat diet and high-fructose water intake, gavage administration for 6 weeks showed that, compared with the high-fat control group, it inhibited more than 70% of the mice's weight gain, with no significant difference in food intake and water intake, demonstrating good clinical application prospects.

The application discloses a fructose probe and a preparation method and application thereof. The fructose probe has a structural formula as shown in formula (I). The fructose probe can be used for high-efficiency affinity enrichment and screening and identification of a fructose direct binding protein, and can provide a new molecular mechanism for glomerular podocyte injury caused by fructose and a new target for treatment of high-fructose related kidney diseases.

The invention relates to the technical field of natural product extraction and purification and biological medicine, in particular to day lily polysaccharide with broad-spectrum antibacterial activity and application of the day lily polysaccharide. The molecular weight of the hemerocallis citrina polysaccharide is 1959 Da, monosaccharide components of the hemerocallis citrina polysaccharide mainly comprise fructose (88.87%) and glucose (11.13%), and the hemerocallis citrina polysaccharide is high-fructose neutral polysaccharide. The main chain is-> 1)-beta-D-Fruf-(2->), side chains are connected to O-6 sites of partial main chain residues, and the side chains comprise beta-D-Fruf-(2->) and-> 6)-beta-D-Fruf-(2->); the molar percentages of the sugar residues Fruf1, 2, Fruf2, Fruf1, 2, 6, Fruf2, 6 and Glc1, 6 are respectively 41.94%, 23.59%, 9.27%, 16.80% and 8.40%, and the molar percentages of the sugar residues Fruf1, 2, Fruf2, Fruf2, Fruf6 and Glc1, 6 are respectively 41.94%, 23.59%, 9.27%, 16.80% and 8.40%; the hemerocallis citrina baroni polysaccharide shows remarkable bacteriostatic activity on clinically separated multi-drug-resistant strains and standard strains, and shows dose dependence; in addition, the improvement effect of the hemerocallis citrina polysaccharide on the lung tissue injury is enhanced along with the increase of the dosage, the effect can be quantified and repeatable, and the requirements of drug development are met.

The present invention discloses a novel fructose utilizing lactic acid producing bacteria Bacillus coagulans FF-7 (MTCC 25235) and its isolation and characterization method. The present invention also discloses the biological applications / therapeutic uses of fructose utilizing lactic acid producing bacteria in increasing utilization of fructose from food and in managing diseases associated with high fructose intake.

This invention belongs to the field of plant genetic engineering technology, specifically involving TRX-M4 Application of gene-related biomaterials in the preparation of products that regulate the soluble sugar content of fruits. Here, regulating the soluble sugar content of fruits refers to increasing or decreasing the content of soluble sugars in the fruit, including fructose, glucose, and sucrose. The present invention provides... TRX-M4 The nucleotide sequence of the gene is shown in SEQ ID NO.3. This invention, through QTL mapping analysis, screened a key candidate gene related to the regulation of sugar content in apple fruit. TRX-M4 Overexpression in Apple TRX-M4 Genes can reduce fructose, glucose, and sucrose content, while silencing them... TRX-M4 TRX-M4 It can increase the content of fructose, glucose and sucrose, thereby promoting the accumulation of several major soluble sugars in genetically modified apples.

The utility model relates to the technical field of high fructose corn syrup processing and conveying, and discloses conveying equipment for high fructose corn syrup processing, which comprises a first base and a second base, a fixed seat is arranged at the top end of the first base, a connecting seat is fixedly connected to the outside of the fixed seat, a conveying table is arranged on one side of the connecting seat, and the conveying table is arranged on the second base. The top end of the conveying table is fixedly connected with a fixing frame, the front end of the exterior of the fixing frame is fixedly connected with an air cylinder, and the rear end of the air cylinder is movably connected with a telescopic rod. According to the conveying equipment for high fructose corn syrup processing, the fixing frame, the air cylinder, the telescopic rod and the clamping plate are arranged, the air cylinder is opened, the telescopic rod can drive the clamping plate to move towards one side, a syrup bottle can be conveniently clamped and positioned, syrup can be conveniently fixed in the conveying process, movement is avoided, and the conveying efficiency is improved. The problem that the syrup bottles are prone to shaking in the conveying process due to the fact that the syrup bottles are inconvenient to clamp and position on a conveying table is solved.

The utility model relates to the technical field of fructose processing and production, and discloses a syrup mixing device for fructose processing, which comprises a stirring tank, a feed pipe is fixedly mounted on one side of the top of the feed pipe, a motor is arranged on the side surface of the feed pipe, and the motor is fixedly connected with the top of the stirring tank through a support rod. A connecting rod A is fixedly installed at the bottom of the feeding pipe, a stirring rod is fixedly installed at the bottom of the connecting rod A, the outer wall of the stirring rod is movably connected with the interior of the stirring tank in a sleeved mode, a temperature sensing part is clamped in one side of the stirring rod, and a heat discharging part is clamped in the side, away from the temperature sensing part, of the stirring rod; according to the scheme, by arranging the heat extraction part, when the temperature in the stirring rod continuously rises, the exhaust fan can be started to extract heat generated in the stirring rod through the pipe B, and the temperature can be reduced during extraction, so that the situation that crystals in syrup are damaged due to continuous temperature rise is avoided, and the production efficiency of fructose can be improved.

The invention relates to the technical field of biology, in particular to a D-psicose 3-epimerase mutant and application thereof.The epimerase mutant is mutated at at least one key site in an amino acid sequence shown in SEQ ID NO: 20, and the mutation is beneficial to improving the catalytic activity of D-psicose formed by D-fructose C3 epimerization; through the mode, the epimerase mutant shows excellent catalytic activity of forming D-psicose through epimerization of D-fructose C3, can efficiently convert D-fructose into D-psicose, and is beneficial to improving the synthesis efficiency of D-psicose.

The invention discloses a food composition for tonifying qi and blood and a preparation method thereof, the food composition comprises water, high fructose syrup, ginseng extract, eucommia ulmoides, gordon euryale seed, medlar, mulberry, raspberry, cordyceps militaris and potassium sorbate, the ginseng extract comprises ginseng, red ginseng and American ginseng, and the ginseng extract comprises ginseng, red ginseng and American ginseng. The preparation method of the food composition comprises the following steps: providing raw materials, boiling with boiling water, mixing and stirring, sterilizing for the first time, filtering, filling and bottling, sterilizing for the second time, and packaging a finished product. The three main raw materials, namely ginseng, red ginseng and American ginseng, are matched with the auxiliary raw materials, and the harmonious and balanced gold proportion of each component is finally obtained through multiple experiments and adjustment, so that the functional effects of tonifying qi and enriching blood can be doubled, the taste during drinking is also improved, and the health-care tea has a good effect of improving the health-care effect. After a user drinks the drink prepared by the invention, the effects of tonifying qi and enriching blood can be effectively enhanced, so that the spirit is improved, and the balance and health of the whole body are favorably promoted.

The application belongs to the technical field of medicine, and particularly relates to application of a 4-(4-diethylamino-1-methylbutylamino)-7-chloroquinoline derivative or a pharmaceutically acceptable salt thereof, or a tautomer thereof, or an internal racemate thereof, or an external racemate thereof, or an enantiomer thereof, or a diastereomer thereof, or a pharmaceutical composition with the active ingredient to preparation of a medicine for preventing or treating obesity and obesity-related metabolic diseases. The 4-(4-diethylamino-1-methylbutylamino)-7-chloroquinoline derivative has a significant curative effect on obesity and related metabolic diseases. After intragastric administration of the 4-(4-diethylamino-1-methylbutylamino)-7-chloroquinoline derivative to obese mice induced by high-fat feed and high-fructose drinking water, after 6 weeks, compared with a high-fat control group, the 4-(4-diethylamino-1-methylbutylamino)-7-chloroquinoline derivative inhibits more than 70% of weight gain of the mice, and there is no significant difference in food intake and water intake, and the 4-(4-diethylamino-1-methylbutylamino)-7-chloroquinoline derivative has a good clinical application prospect.

The utility model relates to the technical field of sugar industry, and discloses an evaporation device for high fructose corn syrup production, which comprises a bottom plate and a motor I. The driving end of the motor I is fixedly connected with a mandrel, the middle end of the outside of the mandrel is fixedly connected with a roller, and the outside of one side of the mandrel is rotatably connected with a support plate. The bottom of the supporting plate is fixedly connected with a supporting table, a fixed shaft is fixedly connected to the interior of the rolling wheel, a connecting rod is rotationally connected to the exterior of the fixed shaft, and a round cover is fixedly connected to one side of the connecting rod. According to the automatic quantitative blanking device, circular motion is converted into linear reciprocating motion through the design of the rolling wheel and the fixed shaft, and the round cover is driven to achieve covering and automatic quantitative blanking; the mandrel, the roller, the fixed shaft and the connecting rod are convenient to disassemble and replace, the maintenance cost is reduced, meanwhile, the strength and stability of the whole structure are improved, the liquid distribution uniformity is improved through the rotary flow dividing layer, a local liquid film is prevented from being too thick and too thin, and therefore the evaporation efficiency is improved, and the heating time and the scaling risk are reduced.

The present invention discloses an aromatic composition comprising: (a) hesperidin dihydrochalcone or a salt thereof; and (b) a high-fructose corn syrup (HFCS) that provides high sweetness but low calorie intake.

The invention provides a preparation method of crystalline fructose, which comprises the following steps: by taking glucose as a raw material, carrying out differential isomerization on glucose isomerase to obtain fructose and glucose mixed liquor, carrying out chromatographic separation on the mixed liquor to obtain high fructose, and carrying out cooling crystallization on the high fructose to finally obtain a high-purity fructose product. The product prepared by the method is high in purity, and the product quality is higher than food-grade and pharmaceutical-grade standards.

The invention relates to the technical field of plant genetic engineering and molecular breeding, and discloses a fructokinase MdFRK1 gene and application thereof in regulation and control of apple fructose accumulation. The apple fructokinase MdFRK1 gene is subjected to naming, structural domain analysis, promoter activity analysis, gene cloning and subcellular localization analysis, recombinant protein is obtained through induced expression of escherichia coli, enzyme activity is detected, fructose content change is analyzed after the MdFRK1 gene is silenced through a VIGS experiment, and results show that the MdFRK1 is mainly localized in cytoplasm, and the MdFRK1 gene can be used for detecting fructose content change. The fructokinase is low-affinity fructokinase, and analysis on activity of the MdFRK1 promoter induced by different sugar sources shows that the activity of the MdFRK1 promoter is the highest under fructose induction; the MdFRK1 gene is silenced through a VIGS experiment, it is found that the activity of fructokinase and the content of fructose are both reduced, and it is found that the activity of fructokinase and the content of fructose are both increased through overexpression of the MdFRK1 gene. According to the invention, the gene positively correlated with the fructose content of the apple is found for the first time, and a new method is provided for culturing the apple with high fructose content.

The present invention relates to an anti-fibrotic composition for preventing or treating fatty liver or liver cirrhosis, the composition containing jujube polysaccharides as an active ingredient. The jujube polysaccharide-containing composition according to the present invention can significantly inhibit the expression of fibrosis-related mRNAs (Tgf-β, Col1a1, Col4a1, Pdgfb, Mmp1, Mmp2, and Timp1) induced in LX2 hepatic stellate cells by TGF-β1, and the whole-cell expression and nucleus expression of fibrosis-related proteins (p-SMAD2, SMAD2, p-SMAD3, SMAD3, and SMAD4). In addition, the jujube polysaccharide-containing composition can significantly suppress body fat mass (fat mass (g), adiposity (% fat)) increased by a high-fat, high-fructose diet (HFHFD), and significantly reduce hepatic steatosis and inflammation. Therefore, the jujube polysaccharide-containing composition according to the present invention is expected to be advantageously used as an anti-fibrotic composition for preventing, alleviating, and treating fatty liver or liver cirrhosis.

The application discloses a low-temperature extraction and blending process of jasmine and lime-flavored stable soda water beverage, and belongs to the technical field of carbonated beverage processing. The application synergistically enhances a composite sweet system of high fructose syrup, acesulfame potassium, cyclamate and aspartame, combines a three-component pH buffer system of citric acid, sodium citrate and sodium bicarbonate, and a triple recombination flavor system of lime, jasmine and tea aroma, and cooperates with 2-8 DEG C low-temperature treatment and nitrogen deoxygenation filling in the whole process, so that the taste coordination and flavor stability of the product are significantly improved. The retention rate of flavor components of the obtained product is greater than or equal to 90%, and the quality is stable within a 12-month shelf life.