Mutant xylose isomerase and its gene and application

A xylose isomerase and amino acid technology, which is applied in the fields of enzyme genetic engineering and enzyme biochemical engineering to achieve the effect of improving activity
CN1966678AInactive Publication Date: 2007-05-23NANJING UNIV OF TECH
1 Cites 10 Cited by

Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
NANJING UNIV OF TECH
Publication Date
2007-05-23
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses mutated xylose isomerase, its coding gene and application. The amino acid sequence of the xylose isomerase is SEQ ID NO.1, SEQ ID NO.3, SEQ ID NO.5 or SEQ ID NO.7. The xylose isomerase has higher activity than wild one, and can be used for high fructose syrup production. The coding gene of the mutated xylose isomerase can be used for the construction of recombinant bacterium utilizing xylose.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention belongs to the fields of genetic engineering of enzymes and enzyme biochemical engineering. The present invention relates to a mutant xylose isomerase with improved activity, and also relates to a gene encoding the mutant xylose isomerase. Background technique

[0002] Xylose isomerase (Xylose Isomerase, XI) (EC 5.3.1.5), also known as glucose isomerase, can catalyze the isomerization reaction of D-xylose to D-xylulose and D-glucose to D-fructose, It plays an important role in the metabolism of sugar in microorganisms and has been used in the industrial production of high fructose syrup [Bhosale SH, Rao MB and Deshpande VV. Molecular and Industrial Aspects of Glucose Isomerase. Microbiol. Rev., 1996, 60 : 280-300]. Xylose isomerase from Thermus thermophilus HB8 [Zeikus JG, Vieille C, Savchenko A. Thermozymes: biotechnology and structure function relationships. Extremophiles, 1998, 2(3): 179-183], the optimum reaction temperature is abou...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More