17 results about "Osmanthus fragrans" patented technology

The invention discloses a method for detecting sweet-scented osmanthus aroma components in sweet-scented osmanthus wine, which comprises the following steps: diluting a wine sample, adding salt until the wine sample is saturated, and then carrying out liquid-liquid micro-extraction by using a mixed solvent of absolute ether and ethyl acetate in a specific ratio, the volume fraction of the ethyl acetate being 3-5%; after oscillation, ultrasonic-assisted extraction and centrifugal separation, an organic phase is taken for dehydration, a gas chromatograph-mass spectrometer is used for analysis, and an internal standard method is adopted for quantification. By optimizing the polarity of the solvent, various aroma components (such as leaf alcohol, linalool oxide, linalool, ionone, tea aroma spirane and the like) from strong polarity to weak polarity can be effectively covered, and balanced and efficient extraction under a single extraction step is realized. The method is easy and convenient to operate, high in recovery rate, good in reproducibility and suitable for quality evaluation and production process control of the osmanthus fragrans wine.

The utility model provides a high-survival-rate cutting seedling raising device for sweet-scented osmanthus seedlings, which belongs to the technical field of cutting seedling raising and comprises a seedling raising shell, and a cutting plate is fixedly connected in the seedling raising shell. The driving groove is formed in the upper end of the seedling raising shell; the two mounting plates are fixedly connected to the upper end of the seedling raising shell; the screw rod is rotationally connected to the close ends of the two mounting plates; the screw nut is in threaded connection with the circumferential surface of the screw rod, the linkage plate is fixedly connected to the lower end of the screw nut, the linkage rod is matched with the sprinkler plate, dynamic matching of the irrigation range and the position of the cuttage plate is achieved, and it is ensured that water evenly covers the root system; a folding leakage-proof plate prevents the nutrient solution from overflowing and maintains a clean environment, a motor drives a lead screw to control a shower plate to move left and right, and a fluorescent lamp is combined for timed light supplement, so that manual intervention is reduced, and the management efficiency is improved.

The invention discloses an osmanthus fragrans cantilever vibration brush picking machine and a method thereof, and relates to the technical field of osmanthus fragrans harvesting equipment, and the robot mainly comprises a mechanical arm, a connecting rod frame and a plurality of rotary sweeping mechanisms which are sequentially connected through adjustable connecting pieces. By adjusting the connecting piece, the multiple rotary sweeping mechanisms can form a profiling structure matched with the outer side contour of the osmanthus tree. During harvesting, the mechanical arm drives the profiling structure to rotate around the tree, and meanwhile a driving part of the rotary sweeping mechanism drives a rotary sweeping shaft provided with a rotary sweeping brush to rotate at a high speed. The method and the device work cooperatively, tight attachment and full-coverage harvesting of irregular crowns are achieved, the problems that traditional manual harvesting is low in efficiency, large in labor intensity, high in harvesting dead angle, prone to damage flowering branches and the like are effectively solved, and the method has the remarkable advantages of being high in harvesting efficiency and automation degree, small in damage to flowers and the like.

The invention relates to the technical field of biology, in particular to a sweet clover MaWRKY76 gene and application thereof.The sweet clover MaWRKY76 gene is cloned firstly, and the expression mode of the sweet clover MaWRKY76 gene under the salt stress condition is analyzed through real-time quantitative PCR; by constructing a yeast heterologous expression vector, the MaWRKY76 is proved to be capable of remarkably improving the salt tolerance of yeast cells. A plant overexpression and RNAi vector is further constructed, sweet clover hairy roots are transformed, and the result shows that the MaWRKY76 not only participates in regulating the symbiotic process of root nodules, but also plays a key role in resisting salt stress. The genetic strategy based on MaWRKY76 can effectively improve symbiotic nitrogen fixation efficiency, plant sustainability and yield of sweet clover under salt stress, provides a theoretical basis for efficient nodulation and nitrogen fixation of leguminous plants under adversity stress, and has positive significance in promoting green sustainable agricultural development.

The application provides a traditional Chinese medicine composition and application thereof in preparation of a drug for treating reflux esophagitis, and relates to the technical field of traditional Chinese medicine pharmacy.The traditional Chinese medicine composition takes Fritillaria thunbergii Miq., Evodia rutaecarpa, Citrus medica L. as monarch drugs, Inula japonica, Terminalia chebula Retz., Nardostachys chinensis, Syzygium aromaticum, Amomum subulatum, Phoenix tree as minister drugs, mother-of-pearl, Cortex Fraxini, Gentiana scabra Bunge, Osmanthus fragrans as auxiliary drugs, and Glycyrrhiza uralensis as a servant drug, and the drugs are combined to have the effects of dispersing the liver and harmonizing the stomach, reducing adverse and acid, and regulating cold and heat.The traditional Chinese medicine composition can significantly improve the esophagitis index of RE rats, has obvious inhibitory effect on inflammatory damage, can significantly improve the pathological morphology of the esophageal tissue of the RE rats, and realizes better anti-inflammatory and therapeutic effect on the RE rats.Meanwhile, the traditional Chinese medicine composition can significantly improve and alleviate the clinical symptoms of the patients with reflux esophagitis, such as acid reflux, heartburn, abdominal distension and belching, and reduce the frequency of the clinical symptoms.The traditional Chinese medicine composition is expected to be further developed and popularized as a drug for treating reflux esophagitis.

The application provides a rhodioloside production strain and a construction method and application thereof, the strain lacks tyrR, feaB, ldhA, ushA, pykF genes, up-regulates tktA , aroG fbr 、 tyrA fbr、 pgm, galU, gntK the transcription level of a gene, and heterologously introduces a TDC gene from Lactobacillus brevis, a TYO gene from Micrococcus luteus, a ARO10 gene from Saccharomyces cerevisiae, and a ADH6 gene, heterologously introduces a GDH gene from Bacillus and a sugar transferase Oft8gt1 gene from Osmanthus fragrans; the construction method is to reconstruct the metabolic network of Escherichia coli to rhodioloside through metabolic engineering and genetic engineering means, which can effectively utilize glucose to synthesize rhodioloside from scratch, improve the production level of rhodioloside, and lay a solid foundation for the industrialized scale production thereof.

This invention discloses a recombinant yeast for producing α-ionone, its construction method, and its applications. The construction method involves using a yeast encoding osmanthus flowers... Osmanthus fragrans The gene for the carotenoid cleavage dioxygenase OfCCD1 was obtained through rational modification and codon optimization. OfCCD1m The gene, with its 5' end fused to the N-terminal membrane-anchored peptide coding sequence of human tyrosine kinase LCK, was transformed into the δ site of Saccharomyces cerevisiae TW3 to obtain recombinant yeast TW4 producing α-ionone. The Saccharomyces cerevisiae strain TW4 producing α-ionone of this invention has the following beneficial effects: it achieves de novo synthesis of α-ionone in Saccharomyces cerevisiae for the first time, filling a technological gap in this field; by using a membrane anchoring strategy to locate the OfCCD1m enzyme in the yeast plasma membrane, the contact efficiency between the enzyme and the substrate ε-carotene is significantly improved, resulting in an α-ionone yield of 1.46 mg / L.

The invention discloses a primer group and a method for specifically detecting osmanthus fragrans ingredients in food and application of the primer group and the method. A base sequence of a forward primer is shown as SEQ ID NO.1; the base sequence of the reverse primer is as shown in SEQ ID NO. 2; and the base sequence of the fluorescent probe is as shown in SEQ ID NO.3. The method has the advantages that the osmanthus fragrans ingredients are specifically detected, and the detection sensitivity of the osmanthus fragrans ingredients reaches 1 pg / mu L; the detection method has strong specificity and repeatability, and can provide an effective technical means for detection of osmanthus fragrans ingredients in the food ingredient detection process.

1. The name of the design product: ceramic ornament (osmanthus fragrans fragrance). 2. The use of the design product: for ornaments, decoration. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective drawing.

The invention discloses an osmanthus fragrans gene OfBBX24 for promoting plant adventitious bud differentiation as well as an expression protein and application thereof, and belongs to the field of plant genetic engineering. The amino acid sequence of the OfBBX24 protein provided by the invention is as shown in SEQ ID NO. 2, and the nucleotide sequence of the encoding gene of the OfBBX24 protein is as shown in SEQ ID NO. 1. According to the invention, a transgenic plant is obtained by cloning the gene, constructing a plant overexpression vector of the gene and transforming tobacco grandiflorum. Experimental results show that overexpression of the OfBBX24 can significantly shorten the differentiation time of the adventitious buds of the transgenic tobacco leaves and improve the differentiation efficiency to 70% or above, which proves that the gene has a strong function of promoting in-vitro regeneration. The invention provides a key gene resource for solving the bottleneck problem that the callus is difficult to differentiate in a woody plant (especially sweet osmanthus) genetic transformation system, and has an important application prospect in forest molecular breeding and efficient propagation technologies.

The invention discloses powdered Fuzhuan tea and a processing method thereof, and belongs to the technical field of deep processing of tea leaves, the processing method of the powdered Fuzhuan tea comprises the following steps: S1, raw material pretreatment: S11, crushing the Fuzhuan tea into tea granules to obtain a primary product which contains eurotium cristatum and can be directly brewed for drinking; s12, respectively drying the dried orange peel and the sweet-scented osmanthus at low temperature, and crushing to obtain dried orange peel powder and sweet-scented osmanthus powder; s2, flavor coupling: mixing the tea granules, the dried orange peel powder and the sweet osmanthus powder in proportion to obtain a second-generation product which does not destroy eurotium cristatum. The primary product obtained by crushing the raw materials at low temperature and the secondary product obtained by flavor coupling can be directly drunk and can retain eurotium cristatum in the Fuzhuan tea, so that beneficial components in the Fuzhuan tea can be reserved to the greatest extent; the powder Fuzhuan tea prepared by reprocessing has convenience, health property and excellent taste and flavor.

The utility model discloses a kind of osmanthus rice brewing fermentation vessels, comprising: container main body, the side wall top surface of container main body is downwardly opened first sealing groove;Container cover, container cover peripheral side is integrally formed with the first sealing piece inserted into first sealing groove;Sealing ring is installed in the first sealing groove, after the first sealing piece is inserted into the first sealing groove, the sealing ring is contacted to make sealing ring deformation contact between the lower bottom surface of first sealing piece and the bottom surface of first sealing groove;The first end of pressurizing part is connected with the container main body pivot, the second end of pressurizing part is opened with limit slot, the container main body is fixed with lock tongue, pressurizing part is rotated by pivot, to make lock tongue enter the limit slot and realize limit;At least one pressurizing part that is bent towards container cover is equipped on the pressurizing part, the lock tongue enters the limit slot to make pressurizing part deformation to pressurize container cover.The utility model structure is simple, sealing effect is good, increase the production efficiency of osmanthus rice brewing.

The application discloses a supercritical fluid extraction device for osmanthus ingredients, which comprises an extraction kettle and a material conveying assembly for conveying supercritical fluid, and a storage assembly is further arranged in the extraction kettle; the storage assembly comprises a storage box, an inner cavity is formed in the storage box, two groups of rotating parts are connected between the storage box and the extraction kettle, a plurality of through holes for the supercritical fluid to pass through are formed in the surface of the storage box, and the plurality of through holes are communicated with the inner cavity of the storage box. The rotatable storage box provided by the supercritical fluid extraction device for osmanthus ingredients is used for the supercritical fluid to flow through the inside of the extraction kettle from bottom to top and to have an extraction reaction with osmanthus; the osmanthus is transferred from the bottom end of the inner cavity of the storage box to the top end of the inner cavity under the pushing of the supercritical fluid; at this time, the storage box is heavy at the top and light at the bottom; and the storage box is turned over under the action of the rotating parts and gravity, so that the osmanthus is located below the inner cavity of the storage box again. The extraction efficiency is ensured, and the situation that the extraction kettle is turned over by 180 degrees is avoided.

This invention discloses a rice-wheat rotation farmland ecosystem based on an intercropping natural enemy conservation zone of Angelica sinensis and Osmanthus fragrans, and a cross-seasonal pest control method. The method involves planting Osmanthus fragrans trees at 3-5 meter intervals along field ridges, with Angelica sinensis interspersed among them, thus constructing a perennial natural enemy conservation zone. During the flowering periods of Angelica sinensis (May-June) and Osmanthus fragrans (September-October), volatile compounds are released, providing nectar and pollen to attract natural enemies such as ladybugs, lacewings, hoverflies, and parasitic wasps to colonize and proliferate. These natural enemy populations naturally migrate into the farmland during the wheat and rice seasons to control pests such as wheat aphids, rice planthoppers, and rice stem borers, with significant control effects and reducing pesticide application by 1-2 times. This perennial system is easy to manage and can generate economic benefits from the harvest of Angelica sinensis and Osmanthus fragrans, providing a technical approach for green agriculture in rice-wheat rotation areas.

The invention discloses an Osmanthus fragrans OfNHX2 gene as well as an expression protein and application thereof, and relates to the technical field of plant genetic engineering. The nucleotide sequence of the Osmanthus fragrans OfNHX2 gene disclosed by the invention is as shown in SEQ ID NO. 1, and the amino acid sequence of the expression protein of the Osmanthus fragrans OfNHX2 gene is as shown in SEQ ID NO. 2. The invention also comprises a recombinant vector containing the gene, a recombinant microorganism and a method for cultivating a salt-sensitive transgenic plant material by using the gene. Experiments show that the leaf relative conductivity, malondialdehyde content, superoxide anion content and hydrogen peroxide content of the transgenic nicotiana benthamiana overexpressed with the OfNHX2 gene are obviously higher than those of a control group under salt stress, and the transgenic nicotiana benthamiana shows a typical non-salt-tolerant phenotype. Therefore, the OfNHX2 gene as well as the expression protein and the application of the OfNHX2 gene provide a brand-new molecular tool and genetic material for plant stress resistance research.

The present application relates to a kind of improving vascular senility composition and its preparation method and application, belong to natural product technical field.The present application provides a kind of improving vascular senility composition, including osmanthus fragrans extract and pumpkin seed protein peptide, the mass ratio of osmanthus fragrans extract and pumpkin seed protein peptide is osmanthus fragrans extract:pumpkin seed protein peptide=1:(0.2-8).The present application is improved vascular senility composition by osmanthus fragrans extract and pumpkin seed protein peptide collocation, can effectively inhibit angiotensin II induced vasoconstriction, enhance cardiac output, prevent zebra fish heart structure and function damage, while also can enhance blood flow velocity, reduce vascular injury, from multidimensional synergistic prevention vascular senility formation.

The invention relates to the technical field of white tea preparation processes, in particular to a dendrobium nobile and lotus leaf compressed white tea preparation process which comprises the following raw materials in parts by weight: 74-76 parts of aged Shomei white tea, 6-7 parts of dried lotus leaves, 4-5 parts of dendrobium nobile granules, 7-8 parts of shredded Xinhui dried tangerine peels, 1-1.5 parts of dried osmanthus fragrans flowers and 4-5 parts of Ningxia Chinese wolfberry petals. When the dried orange peel component and the base material are fermented together, active substances such as pectin and naringin can be quickly released, part of astringent substances in the lotus leaves and the tea leaves are effectively wrapped and converted into quick salivation promoting feeling, the mellowness of tea soup is remarkably improved, volatile terpene aromatic oil of sweet-scented osmanthus is locked through a high osmotic pressure environment, and the taste of the sweet-scented osmanthus is improved. In the curing process, flower fragrance molecules and sugar are promoted to generate primary Maillard reaction precursor change, the fresh and sharp faint scent is converted into deeper and more stable cooked sweet fragrance, and the baked Chinese wolfberry petals slowly release mild sweet substances and nourishing components in the fermentation process.