57 results about "Osmanthus fragrans" patented technology

The invention belongs to the technical field of plant essential oil, and particularly relates to osmanthus essential oil and application thereof in preparation of pure natural children shampoo capable of removing dandruff, relieving itching and controlling oil. By optimizing the extraction process of the sweet osmanthus essential oil and adopting a method of combining enzymolysis pretreatment with reflux extraction, the extraction efficiency and the active ingredient content of the sweet osmanthus essential oil are remarkably improved. Compared with a traditional extraction method, the extraction process disclosed by the invention has the advantages that natural active ingredients, such as linalool, beta-glucoside and the like, in the osmanthus fragrans essential oil can be better reserved, so that the antibacterial and oil control effects are improved. The osmanthus essential oil is applied to the shampoo for children, so that mild and efficient scalp care can be provided for children. Compared with the traditional shampoo, the shampoo for children not only can clean hair, but also can remarkably reduce the problems of scalp itching, dandruff, excessive grease secretion and the like, and meanwhile, the irritation of chemical components to the scalp of children is avoided.

The invention relates to a turmeric enzyme beverage and a preparation method thereof, and belongs to the field of fermented beverages. The turmeric enzyme beverage is prepared from the following raw materials: curcumin, blueberries, mulberries, apples, radix polygonati officinalis and herba elsholtziae. According to the invention, blueberries, mulberries and apples are adopted to provide excellent nutrition, taste and flavor for the enzyme beverage, and the enzyme beverage also has a certain health-care effect; the curcumin, the polygonatum odoratum and the elsholtzia chinensis can remarkably improve the immunity of a human body; the enzyme beverage prepared by the preparation method disclosed by the invention is excellent in effect and has a relatively good application prospect.

The invention provides a Mongolian medicine composition for treating hepatitis B and a preparation method of the Mongolian medicine composition. The composition is prepared from the following components in parts by weight: 75 to 85 parts of saffron, 75 to 85 parts of trogopterus dung, 65 to 75 parts of gardenia jasminoides, 45 to 55 parts of cortex phellodendri, 45 to 55 parts of dianthus chinensis, 55 to 65 parts of gypsum, 55 to 65 parts of scabiosa, 55 to 65 parts of calamine, 55 to 65 parts of caulis akebiae, 25 to 35 parts of rhizoma coptidis, 25 to 35 parts of fructus gardeniae, 25 to 35 parts of calomel, 25 to 35 parts of bunge corydalis herb, 45 to 55 parts of elecampane, 45 to 55 parts of rose and 55 to 65 parts of calculus bovis factitius. The Mongolian medicine composition for treating hepatitis B is prepared from multiple raw materials, meanwhile, the added coptis chinensis and rose flowers are treated through a specific extraction mode and a specific fermentation mode, and the prepared Mongolian medicine composition for treating hepatitis B has an excellent hepatitis B treatment effect.

The invention relates to an osmanthus protoplast preparation method and an instantaneous conversion method. The preparation method of the sweet-scented osmanthus protoplast comprises the following steps: collecting different tissues of fresh sweet-scented osmanthus, performing surface disinfection and sterilization, cutting into thin strips or slices, adding enzymatic hydrolysate for enzymolysis, filtering, centrifuging, washing with a W5 solution, and re-suspending. The instantaneous transformation method of the osmanthus protoplast comprises the following steps: adjusting the concentration of the osmanthus protoplast by using an MMG solution, adding plasmids for mixing, adding a PEG solution for transformation incubation, adding a W5 solution for washing and centrifuging, and detecting the instantaneous transformation efficiency of the osmanthus protoplast. According to the method, a high-quality protoplast separation system of the leaves, the stems and the petals of the sweet-scented osmanthus is successfully established, efficient conversion of mesophyll and petal protoplast is realized for the first time, and a reliable technical platform is provided for molecular biology research of the sweet-scented osmanthus.

The invention discloses an osmanthus fragrans Don sour tea making method which is characterized by comprising the following steps: raw material preparation: selecting fresh and complete tea leaves without plant diseases and insect pests and osmanthus fragrans; after being accepted and accepted, the fresh leaves are spread on a dustpan or a bamboo basketry in time, and water evaporation is accelerated; one of boiling water fixation, steam fixation or stir-frying fixation is adopted; quickly cooling the enzyme-deactivated leaves to room temperature by adopting a spreading and airing rack, a platform and a bamboo strip mat, wherein the water content of the tea leaves is 55-68%; rolling and mixing the tea leaves and the sweet-scented osmanthus in proportion; loading the rolled tea and cassia bark mixture into the pretreated fermentation container, compacting and sealing for anaerobic fermentation; taking out the tea leaves after fermentation, pounding the tea leaves into tea paste in a stone hammer, kneading the tea paste into dragon beads or pressing the tea paste into round pieces, and drying the tea leaves in the sun until the water content of the tea leaves is less than or equal to 13%. The method solves the problems that traditional bamboo tube fermentation easily generates urn flavor and canned fermentation cannot effectively fuse osmanthus fragrans flavor, realizes natural and lasting fusion of osmanthus fragrans flavor, and improves tea quality and flavor level.

The invention discloses a tree species screening method for heavy metal lead contaminated soil remediation, and relates to the technical field of heavy metal lead contaminated ecological management. Common greening tree species such as cinnamomum camphora, willow, gingko, koelreuteria paniculata, photinia serrulata and osmanthus fragrans are selected, leaf and rhizosphere soil are collected in a point distribution mode, and the content of heavy metal Pb in a sample is measured; the heavy metal Pb content distribution characteristics of different tree species are quantified, the heavy metal Pb enrichment capacity difference of the greening tree species is explored, the heavy metal Pb enrichment and transfer efficiency of the greening tree species is comprehensively evaluated, and the greening tree species with the best heavy metal Pb purification effect is screened out. The method is a phytoremediation technology which has the advantages of being economical, simple, convenient, high in recognition degree, long in life cycle, free of damage to the soil structure, not prone to causing secondary pollution and the like.

The invention discloses a method for detecting sweet-scented osmanthus aroma components in sweet-scented osmanthus wine, which comprises the following steps: diluting a wine sample, adding salt until the wine sample is saturated, and then carrying out liquid-liquid micro-extraction by using a mixed solvent of absolute ether and ethyl acetate in a specific ratio, the volume fraction of the ethyl acetate being 3-5%; after oscillation, ultrasonic-assisted extraction and centrifugal separation, an organic phase is taken for dehydration, a gas chromatograph-mass spectrometer is used for analysis, and an internal standard method is adopted for quantification. By optimizing the polarity of the solvent, various aroma components (such as leaf alcohol, linalool oxide, linalool, ionone, tea aroma spirane and the like) from strong polarity to weak polarity can be effectively covered, and balanced and efficient extraction under a single extraction step is realized. The method is easy and convenient to operate, high in recovery rate, good in reproducibility and suitable for quality evaluation and production process control of the osmanthus fragrans wine.

The utility model belongs to the technical field of pastry processing equipment, and particularly relates to a multi-station sweet-scented osmanthus scattering machine for pastry processing, which comprises a rack, a sweet-scented osmanthus hopper is welded at the upper end of the rack, a stirring device is arranged on the sweet-scented osmanthus hopper, a support is mounted above the rack through bolts, a mounting plate is welded at the top of the support, and the mounting plate is arranged on the rack. A grabbing mechanism is arranged below the mounting plate, a discharging flat plate is mounted at the upper end of the rack and located in front of the sweet osmanthus hopper through bolts, and an air pipe valve is mounted on the vertical portion of the discharging flat plate. By arranging an osmanthus fragrans hopper, a material placing flat plate, a material scattering disc and a material grabbing mechanism, osmanthus fragrans raw materials are placed in the osmanthus fragrans hopper to be stored, then grabbed through the material grabbing mechanism, then horizontally moved to the material placing flat plate, pneumatically blown to the position above the material scattering disc and finally evenly distributed through the material scattering disc, and then the osmanthus fragrans raw materials fall on pastries on a conveying belt below. Multiple stations are carried out at the same time, efficiency is high, and flower scattering is uniform.

The invention discloses a herbal hair growing and growing composition and a preparation method thereof, and belongs to the technical field of compositions. Comprising a hair growing component and a herbaceous plant additive, the herbaceous plant auxiliary agent comprises a pea seedling extract, a ginger extract, a wiss grass extract, a girald pteroxygonum root extract, a rosemary extract, a crithmum maritimum extract, a fructus cnidii extract, a nigella nigra seed extract and a Sinkiang lithospermum extract; the hair growing component comprises herba ecliptae, herba cistanche, flos carthami, radix polygoni multiflori, folium mori, fructus ligustri lucidi, Chinese soapberry fruit, cacumen biotae, Chinese honey locust, tartary buckwheat extract, cortex mori radicis, honeysuckle stem and rhizoma drynariae. Through synergistic interaction of all the components, the hair-growing shampoo has good hair-growing and hair-growing effects.

The invention provides a Pistacia weinmannifolia extract-containing skin care liquid perfumed soap for diabetics, and relates to the technical field of perfumed soap, the Pistacia weinmannifolia extract-containing skin care liquid perfumed soap for diabetics comprises Pistacia weinmannifolia essential oil, a Pistacia weinmannifolia extract, a surfactant, a synergistic skin care adjuvant, a pH regulator and deionized water, one purpose of the present invention is to provide a liquid soap composition, which comprises a Pistacia weinmannifolia extract and essential oil, and is matched with a mild soap base and an optional synergistic skin care adjuvant so as to provide a safe and effective skin care choice for diabetic patients.

The invention belongs to the field of beverage preparation, and particularly relates to a cold-bone crystal ball beverage treated by ultrahigh pressure and a preparation method of the cold-bone crystal ball beverage. A viscous compound containing konjaku flour, agar, carrageenan and sodium alginate interacts with calcium ions in a cross-linking solution, so that the overall structural stability of the prepared chill crystal balls is enhanced, the elasticity and chewiness are improved, and the chill crystal balls are prevented from being mutually adhered; due to a network structure contained in the components of the suspension, the release speed of flavor components in the flavor milk and the fruit composition contained in the beverage base solution is slowed down, and the uniformity of the beverage is maintained for a long time; the stability maintaining solution containing vitamin E, polydextrose and lactic acid is mixed with the cold-bone pearl crystal ball beverage subjected to ultrahigh pressure treatment, after drinking, the blood sugar and blood fat cannot be greatly increased, the contained cold-bone crystal balls are good in elasticity, high in brittleness and good in chewiness, the cold-bone crystal balls cannot be adhered after being stored, and the flavor is maintained for a long time.

The utility model provides a high-survival-rate cutting seedling raising device for sweet-scented osmanthus seedlings, which belongs to the technical field of cutting seedling raising and comprises a seedling raising shell, and a cutting plate is fixedly connected in the seedling raising shell. The driving groove is formed in the upper end of the seedling raising shell; the two mounting plates are fixedly connected to the upper end of the seedling raising shell; the screw rod is rotationally connected to the close ends of the two mounting plates; the screw nut is in threaded connection with the circumferential surface of the screw rod, the linkage plate is fixedly connected to the lower end of the screw nut, the linkage rod is matched with the sprinkler plate, dynamic matching of the irrigation range and the position of the cuttage plate is achieved, and it is ensured that water evenly covers the root system; a folding leakage-proof plate prevents the nutrient solution from overflowing and maintains a clean environment, a motor drives a lead screw to control a shower plate to move left and right, and a fluorescent lamp is combined for timed light supplement, so that manual intervention is reduced, and the management efficiency is improved.

The invention discloses an osmanthus fragrans cantilever vibration brush picking machine and a method thereof, and relates to the technical field of osmanthus fragrans harvesting equipment, and the robot mainly comprises a mechanical arm, a connecting rod frame and a plurality of rotary sweeping mechanisms which are sequentially connected through adjustable connecting pieces. By adjusting the connecting piece, the multiple rotary sweeping mechanisms can form a profiling structure matched with the outer side contour of the osmanthus tree. During harvesting, the mechanical arm drives the profiling structure to rotate around the tree, and meanwhile a driving part of the rotary sweeping mechanism drives a rotary sweeping shaft provided with a rotary sweeping brush to rotate at a high speed. The method and the device work cooperatively, tight attachment and full-coverage harvesting of irregular crowns are achieved, the problems that traditional manual harvesting is low in efficiency, large in labor intensity, high in harvesting dead angle, prone to damage flowering branches and the like are effectively solved, and the method has the remarkable advantages of being high in harvesting efficiency and automation degree, small in damage to flowers and the like.

The invention belongs to the technical field of biological pharmacy, and particularly relates to a sweet osmanthus lipstick for preventing and treating cheilitis and fading cheilogramma. In the composition disclosed by the invention, the osmanthus fragrans extract can reduce transcription of proinflammatory factors by inhibiting two signal channels of NF-kB and AP-1, so that generation of inflammatory mediators is reduced; the bisabolol has remarkable anti-inflammatory and soothing effects and can effectively relieve inflammation symptoms such as red and swollen lips and pain. The radix arnebiae seu lithospermi extract has the effects of clearing away heat and toxic materials and cooling and activating blood, and further enhances the anti-inflammatory effect. Phytosphingosine can promote repair and regeneration of lip cells and accelerate healing of damaged lip skin.

The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a traditional Chinese medicine composition for protecting gastric mucosa and a preparation method thereof. Comprising 10-12 parts of poria cocos, 8-10 parts of pericarpium citri reticulatae, 2-4 parts of scorched hawthorn fruit, 2-4 parts of hypericum perforatum, 2-4 parts of fried medicated leaven, 5-8 parts of sweet-scented osmanthus, 2-4 parts of amomum cardamomum, 0-20 parts of rhizoma bletillae, 0-15 parts of cuttlebone, 0-15 parts of astragalus membranaceus, 0-15 parts of hairyvein agrimony, 0-6 parts of pseudo-ginseng, 0-12 parts of dandelion, 0-5 parts of dried ginger, 0-9 parts of smoked plum and 0-8 parts of liquorice. The comprehensiveness and the safety of the formula are ensured. The traditional Chinese medicine composition for protecting the gastric mucosa, provided by the invention, ensures the comprehensiveness and safety of the formula from the aspects of enhancing the curative effect, adapting to the syndrome type, avoiding side effects and the like through the synergistic effect of various medicinal materials, and is suitable for preventing and repairing gastric mucosa lesion.

The invention discloses a pharmaceutical composition for rhinitis health care and respiratory tract circulation improvement and a preparation method thereof.The pharmaceutical composition is prepared from pogostemon cablin, osmanthus fragrans, geranium, wild chrysanthemum flowers, semen armeniacae amarae, astragalus membranaceus, pericarpium citri reticulatae, platycodon grandiflorum, mint and menthol through processing and liquefying, and through the synergistic effect of all the pharmaceutical ingredients, the pharmaceutical composition has the effects of inducing resuscitation with fragrance, dissolving turbidity and dredging resuscitation; the condition of unsmooth ventilation of the nose can be effectively improved, and uncomfortable symptoms such as nasal obstruction can be relieved; the spreading and descending of lung qi are regulated, and the normal physiological function of the respiratory tract is maintained; the whole resistance of the body and local qi and blood nourishing of the nose are enhanced, and repair and regeneration of nose tissues are promoted; the invasion of external heat toxin to the nose is resisted, and the inflammatory response is relieved. Long-term use of the nasal mask can significantly promote respiratory tract microcirculation, enhance the immune defense function of the nose, and effectively prevent occurrence and recurrence of nasal diseases.

The invention relates to the technical field of biology, in particular to a sweet clover MaWRKY76 gene and application thereof.The sweet clover MaWRKY76 gene is cloned firstly, and the expression mode of the sweet clover MaWRKY76 gene under the salt stress condition is analyzed through real-time quantitative PCR; by constructing a yeast heterologous expression vector, the MaWRKY76 is proved to be capable of remarkably improving the salt tolerance of yeast cells. A plant overexpression and RNAi vector is further constructed, sweet clover hairy roots are transformed, and the result shows that the MaWRKY76 not only participates in regulating the symbiotic process of root nodules, but also plays a key role in resisting salt stress. The genetic strategy based on MaWRKY76 can effectively improve symbiotic nitrogen fixation efficiency, plant sustainability and yield of sweet clover under salt stress, provides a theoretical basis for efficient nodulation and nitrogen fixation of leguminous plants under adversity stress, and has positive significance in promoting green sustainable agricultural development.

The invention relates to the field of poultry medicines and poultry medicine preparation, and particularly discloses application of turpinia arguta leaf superfine powder in preparation of a riemerella anatipestifer disease prevention product. The turpinia arguta leaf superfine powder provided by the invention has the effects of clearing away heat and toxic materials, resisting bacteria and diminishing inflammation and the like; the traditional Chinese medicine superfine powder is simple to prepare, low in cost, high in safety and free of side effects, and can reduce or replace the use of antibiotics, so that the problems of drug resistance and drug residues are reduced; by using the turpinia arguta leaf superfine powder disclosed by the invention, the riemerella anatipestifer disease of the meat ducks can be remarkably prevented.

The present invention discloses an Osmanthus fragrans OfSVB1 gene, its expressed protein, and applications, and relates to the technical field of plant genetic engineering. The nucleotide sequence of the Osmanthus fragrans OfSVB1 gene disclosed in the present invention is shown in SEQ ID NO.1, and the amino acid sequence of its expressed protein is shown in SEQ ID NO.2. The present invention constructs an expression vector for the Osmanthus fragrans OfSVB1 gene; transforms the constructed expression vector for the Osmanthus fragrans OfSVB1 gene into Nicotiana benthamiana; and cultivates, screens, and obtains transgenic Nicotiana benthamiana plants with improved salt stress resistance. The results of the examples of the present invention show that after being subjected to high salt stress, the relative conductivity and proline content of the plants overexpressing the OfSVB1 gene decreased, while the POD activity increased significantly, indicating that overexpressing the OfSVB1 gene delayed leaf aging and enabled plant tissues to synthesize more POD to cope with adversity.

The application provides a traditional Chinese medicine composition and application thereof in preparation of a drug for treating reflux esophagitis, and relates to the technical field of traditional Chinese medicine pharmacy.The traditional Chinese medicine composition takes Fritillaria thunbergii Miq., Evodia rutaecarpa, Citrus medica L. as monarch drugs, Inula japonica, Terminalia chebula Retz., Nardostachys chinensis, Syzygium aromaticum, Amomum subulatum, Phoenix tree as minister drugs, mother-of-pearl, Cortex Fraxini, Gentiana scabra Bunge, Osmanthus fragrans as auxiliary drugs, and Glycyrrhiza uralensis as a servant drug, and the drugs are combined to have the effects of dispersing the liver and harmonizing the stomach, reducing adverse and acid, and regulating cold and heat.The traditional Chinese medicine composition can significantly improve the esophagitis index of RE rats, has obvious inhibitory effect on inflammatory damage, can significantly improve the pathological morphology of the esophageal tissue of the RE rats, and realizes better anti-inflammatory and therapeutic effect on the RE rats.Meanwhile, the traditional Chinese medicine composition can significantly improve and alleviate the clinical symptoms of the patients with reflux esophagitis, such as acid reflux, heartburn, abdominal distension and belching, and reduce the frequency of the clinical symptoms.The traditional Chinese medicine composition is expected to be further developed and popularized as a drug for treating reflux esophagitis.

The invention relates to the technical field of Tibetan medicines, in particular to a preparation containing Tibetan medicine musk, a preparation method of the preparation and application of the preparation in preparation of liver cancer medicines. The preparation is prepared from the following raw materials: musk, swertia mussotii, rheum tanguticum, dracocephalum tanguticum, delphinium delphinium, phyllanthus emblica, salviae miltiorrhizae, herpetospermum pedunculosum, saussurea hypoglauca, saffron crocus, trogopterus dung, fructus chebulae and plantago asiatica. According to the invention, a plurality of medicinal materials are compatible, all the raw materials have a synergistic effect, the effects of compound components are effectively exerted, and the traditional Chinese medicine composition can resist cancer, protect liver, clear away heat and toxic materials and inhibit tumor growth, has a good anti-liver cancer effect and does not generate an immunosuppressive effect on a body.

The invention relates to the technical field of nano material preparation, and discloses a preparation process of ethanol solvent mediated osmanthus fragrans extract surface functionalized nano Fe3O4, which comprises the following steps: preparing a mixed solution of ferric chloride and ferrous chloride, and ensuring that the total concentration of iron ions is 0.1 M; dissolving the osmanthus fragrans extract flavone in absolute ethyl alcohol; adding the flavone solution into the mixed solution; putting the mixed solution into a high-pressure reaction kettle; after the reaction is completed, cooling for more than 12 hours; centrifuging the cooled mixture, and collecting a lower-layer precipitate; and carrying out water washing and drying treatment on the obtained precipitate to obtain the nano Fe3O4 with the surface functionalized osmanthus fragrans extract. According to the invention, active components in the osmanthus fragrans extract are covalently modified on the surface of nano Fe3O4, so that the nano Fe3O4 is endowed with anti-tumor, anti-inflammatory, anti-oxidation and other biological activities, meanwhile, superparamagnetism is retained, the preparation process is simplified, the production cost is reduced, toxic reagents such as ammonia water do not need to be used, and the green chemistry concept is met.

The invention discloses an osmanthus fragrans extract for preventing premature ovarian failure and a preparation method and application thereof.The extract is prepared from osmanthus fragrans through microwave fixation, gradient temperature weak acid extraction, membrane separation and concentration, low-temperature drying and other pure physical processes, effective components can be prevented from being damaged, the process is stable, green and efficient, the active components are definite, and the preparation method is simple. Wherein the sum of the contents of the salidroside, the verbascoside and the ligustroside is not less than 20%. Animal experiments show that AMH, FSH and LH hormone levels in mice suffering from premature ovarian failure induced by cyclophosphamide can be remarkably improved, the ovarian function can be effectively improved, all index components including salidroside, verbascoside and ligustrum glycoside have a synergistic interaction effect, a safe and effective solution scheme derived from natural plants is provided for preventing premature ovarian failure, and the application prospect is wide. The method can be widely applied to preparation of various products.

The application provides a rhodioloside production strain and a construction method and application thereof, the strain lacks tyrR, feaB, ldhA, ushA, pykF genes, up-regulates tktA , aroG fbr 、 tyrA fbr、 pgm, galU, gntK the transcription level of a gene, and heterologously introduces a TDC gene from Lactobacillus brevis, a TYO gene from Micrococcus luteus, a ARO10 gene from Saccharomyces cerevisiae, and a ADH6 gene, heterologously introduces a GDH gene from Bacillus and a sugar transferase Oft8gt1 gene from Osmanthus fragrans; the construction method is to reconstruct the metabolic network of Escherichia coli to rhodioloside through metabolic engineering and genetic engineering means, which can effectively utilize glucose to synthesize rhodioloside from scratch, improve the production level of rhodioloside, and lay a solid foundation for the industrialized scale production thereof.

The present invention discloses miRNA internal reference genes of different tissues of sweet osmanthus and primers and applications thereof, and belongs to the field of plant molecular biology. The present invention finally selects 14 required candidate internal references according to literature review, database search and small RNA sequencing, and designs real-time fluorescence quantitative PCR primers for internal references. The stability of candidate internal references is evaluated by 5 algorithms (delta-CT, geNorm, NormFinder, BestKeeper and RefFinder), and the best miRNA internal references for fluorescence quantification of different tissues of sweet osmanthus are obtained, which are novel2 and ofr-miR395e. The present invention fills the technical gap of no miRNA fluorescence quantification internal reference in different tissues of sweet osmanthus, provides strong support for the quantitative expression of functional miRNA related to different tissues of sweet osmanthus, and thus has important application value.

The invention discloses a formula and a preparation method of a natural anti-alcoholism beverage. The formula comprises soybean sprouts, dried longan, fresh ginger, radix puerariae, citrus, sweet osmanthus, natural cinnamon essential oil and honey. The preparation method comprises the following steps: selecting high-quality soybeans and culturing; smashing and mixing the dried longan and soybean sprouts, adding purified water, boiling and filtering, and collecting an extracting solution to obtain a mother solution A; crushing fresh ginger, mixing with radix puerariae, adding an ethanol solution, performing ultrasonic-assisted extraction, performing cold soaking, performing suction filtration, combining three extracting solutions, performing low-pressure rotary evaporation concentration, and removing alcohol to obtain mother liquor B; putting the citrus and osmanthus fragrans into a fermentation tank, adding distilled water, inoculating acetic bacteria and hansenula anomala according to a ratio for fermentation, and filtering out pomace to obtain mother liquor C; mixing the mother liquor A, the mother liquor B and the mother liquor C, adding cinnamon essential oil and honey, stirring at a high speed, canning and sterilizing to obtain the beverage with the hangover alleviating function. After drunkenness, the traditional Chinese medicine composition can remarkably improve the in-vivo metabolism speed of alcohol and remarkably and effectively relieve symptoms such as headache and stomach discomfort caused by drunkenness.

The application discloses a solid osmanthus fragrans concentrate powder beverage, a preparation method and application. In recent years, with the intensification of social competition and the continuous acceleration of life rhythm, stress has become an important factor affecting the health status of modern people. The total weight of the osmanthus fragrans concentrate powder beverage comprises 99.5% of osmanthus fragrans concentrate powder and 0.5% of food additive; dry osmanthus fragrans is immersed in warm water for three times, the three times of extraction liquid are combined, filtered, concentrated under reduced pressure, spray dried, sieved and packaged. The application adopts a low-temperature extraction process and a concentration process, has the effects of resisting fatigue and preventing senile dementia, and can be quickly dissolved in cold or warm water, which is convenient and fast.

This invention discloses a recombinant yeast for producing α-ionone, its construction method, and its applications. The construction method involves using a yeast encoding osmanthus flowers... Osmanthus fragrans The gene for the carotenoid cleavage dioxygenase OfCCD1 was obtained through rational modification and codon optimization. OfCCD1m The gene, with its 5' end fused to the N-terminal membrane-anchored peptide coding sequence of human tyrosine kinase LCK, was transformed into the δ site of Saccharomyces cerevisiae TW3 to obtain recombinant yeast TW4 producing α-ionone. The Saccharomyces cerevisiae strain TW4 producing α-ionone of this invention has the following beneficial effects: it achieves de novo synthesis of α-ionone in Saccharomyces cerevisiae for the first time, filling a technological gap in this field; by using a membrane anchoring strategy to locate the OfCCD1m enzyme in the yeast plasma membrane, the contact efficiency between the enzyme and the substrate ε-carotene is significantly improved, resulting in an α-ionone yield of 1.46 mg / L.

The invention discloses a primer group and a method for specifically detecting osmanthus fragrans ingredients in food and application of the primer group and the method. A base sequence of a forward primer is shown as SEQ ID NO.1; the base sequence of the reverse primer is as shown in SEQ ID NO. 2; and the base sequence of the fluorescent probe is as shown in SEQ ID NO.3. The method has the advantages that the osmanthus fragrans ingredients are specifically detected, and the detection sensitivity of the osmanthus fragrans ingredients reaches 1 pg / mu L; the detection method has strong specificity and repeatability, and can provide an effective technical means for detection of osmanthus fragrans ingredients in the food ingredient detection process.

The invention provides a traditional Chinese medicine tea composition for improving viral pneumonia and a preparation method and application thereof, and relates to the technical field of traditional Chinese medicine, the traditional Chinese medicine tea composition comprises the following components: 11-18 parts of perilla leaf, 7-13 parts of honeysuckle, 7-13 parts of polygonatum odoratum, 5-12 parts of scorched hawthorn fruit, 4-8 parts of platycodon grandiflorum, 3-7 parts of pericarpium citri reticulatae, 3-7 parts of liquorice, 3-7 parts of chrysanthemum, 2-5 parts of osmanthus fragrans, 0.3-0.6 part of stevia rebaudiana, and 6-14 parts of black tea. An anti-inflammatory effect experiment of THP-1 cells proves that the traditional Chinese medicine tea composition has an improvement effect on viral pneumonia, specifically, the traditional Chinese medicine tea composition can effectively reduce IL-6 expression and has good anti-inflammatory activity; the expression of inflammatory indexes NO and IL-6 of RAW264.7 cells induced by lipopolysaccharide LPS is obviously reduced; the traditional Chinese medicine tea composition can be applied to preparation of anti-virus pneumonia products.