6 results about "Osmanthus fragrans" patented technology

The application provides a rhodioloside production strain and a construction method and application thereof, the strain lacks tyrR, feaB, ldhA, ushA, pykF genes, up-regulates tktA , aroG fbr 、 tyrA fbr、 pgm, galU, gntK the transcription level of a gene, and heterologously introduces a TDC gene from Lactobacillus brevis, a TYO gene from Micrococcus luteus, a ARO10 gene from Saccharomyces cerevisiae, and a ADH6 gene, heterologously introduces a GDH gene from Bacillus and a sugar transferase Oft8gt1 gene from Osmanthus fragrans; the construction method is to reconstruct the metabolic network of Escherichia coli to rhodioloside through metabolic engineering and genetic engineering means, which can effectively utilize glucose to synthesize rhodioloside from scratch, improve the production level of rhodioloside, and lay a solid foundation for the industrialized scale production thereof.

This invention discloses a recombinant yeast for producing α-ionone, its construction method, and its applications. The construction method involves using a yeast encoding osmanthus flowers... Osmanthus fragrans The gene for the carotenoid cleavage dioxygenase OfCCD1 was obtained through rational modification and codon optimization. OfCCD1m The gene, with its 5' end fused to the N-terminal membrane-anchored peptide coding sequence of human tyrosine kinase LCK, was transformed into the δ site of Saccharomyces cerevisiae TW3 to obtain recombinant yeast TW4 producing α-ionone. The Saccharomyces cerevisiae strain TW4 producing α-ionone of this invention has the following beneficial effects: it achieves de novo synthesis of α-ionone in Saccharomyces cerevisiae for the first time, filling a technological gap in this field; by using a membrane anchoring strategy to locate the OfCCD1m enzyme in the yeast plasma membrane, the contact efficiency between the enzyme and the substrate ε-carotene is significantly improved, resulting in an α-ionone yield of 1.46 mg / L.

The utility model discloses a kind of osmanthus rice brewing fermentation vessels, comprising: container main body, the side wall top surface of container main body is downwardly opened first sealing groove;Container cover, container cover peripheral side is integrally formed with the first sealing piece inserted into first sealing groove;Sealing ring is installed in the first sealing groove, after the first sealing piece is inserted into the first sealing groove, the sealing ring is contacted to make sealing ring deformation contact between the lower bottom surface of first sealing piece and the bottom surface of first sealing groove;The first end of pressurizing part is connected with the container main body pivot, the second end of pressurizing part is opened with limit slot, the container main body is fixed with lock tongue, pressurizing part is rotated by pivot, to make lock tongue enter the limit slot and realize limit;At least one pressurizing part that is bent towards container cover is equipped on the pressurizing part, the lock tongue enters the limit slot to make pressurizing part deformation to pressurize container cover.The utility model structure is simple, sealing effect is good, increase the production efficiency of osmanthus rice brewing.

The application discloses a supercritical fluid extraction device for osmanthus ingredients, which comprises an extraction kettle and a material conveying assembly for conveying supercritical fluid, and a storage assembly is further arranged in the extraction kettle; the storage assembly comprises a storage box, an inner cavity is formed in the storage box, two groups of rotating parts are connected between the storage box and the extraction kettle, a plurality of through holes for the supercritical fluid to pass through are formed in the surface of the storage box, and the plurality of through holes are communicated with the inner cavity of the storage box. The rotatable storage box provided by the supercritical fluid extraction device for osmanthus ingredients is used for the supercritical fluid to flow through the inside of the extraction kettle from bottom to top and to have an extraction reaction with osmanthus; the osmanthus is transferred from the bottom end of the inner cavity of the storage box to the top end of the inner cavity under the pushing of the supercritical fluid; at this time, the storage box is heavy at the top and light at the bottom; and the storage box is turned over under the action of the rotating parts and gravity, so that the osmanthus is located below the inner cavity of the storage box again. The extraction efficiency is ensured, and the situation that the extraction kettle is turned over by 180 degrees is avoided.

This invention discloses a rice-wheat rotation farmland ecosystem based on an intercropping natural enemy conservation zone of Angelica sinensis and Osmanthus fragrans, and a cross-seasonal pest control method. The method involves planting Osmanthus fragrans trees at 3-5 meter intervals along field ridges, with Angelica sinensis interspersed among them, thus constructing a perennial natural enemy conservation zone. During the flowering periods of Angelica sinensis (May-June) and Osmanthus fragrans (September-October), volatile compounds are released, providing nectar and pollen to attract natural enemies such as ladybugs, lacewings, hoverflies, and parasitic wasps to colonize and proliferate. These natural enemy populations naturally migrate into the farmland during the wheat and rice seasons to control pests such as wheat aphids, rice planthoppers, and rice stem borers, with significant control effects and reducing pesticide application by 1-2 times. This perennial system is easy to manage and can generate economic benefits from the harvest of Angelica sinensis and Osmanthus fragrans, providing a technical approach for green agriculture in rice-wheat rotation areas.

The present application relates to a kind of improving vascular senility composition and its preparation method and application, belong to natural product technical field.The present application provides a kind of improving vascular senility composition, including osmanthus fragrans extract and pumpkin seed protein peptide, the mass ratio of osmanthus fragrans extract and pumpkin seed protein peptide is osmanthus fragrans extract:pumpkin seed protein peptide=1:(0.2-8).The present application is improved vascular senility composition by osmanthus fragrans extract and pumpkin seed protein peptide collocation, can effectively inhibit angiotensin II induced vasoconstriction, enhance cardiac output, prevent zebra fish heart structure and function damage, while also can enhance blood flow velocity, reduce vascular injury, from multidimensional synergistic prevention vascular senility formation.