The invention discloses a composition for preparing a health-care and body-building medicinal starter as well as a preparation method and application of the composition. The composition is prepared from the following raw materials of agilawood, sandalwood, artificial ambergris, artificial musk, radix angelicae sinensis, radix astragali, rhizoma gastrodiae, saffron crocus, rhizoma chuanxiong, flos caryophylli, cortex cinnamomi, myrrh, frankincense, fructus amomi, caulis akebiae, radix aucklandiae, agastache rugosus, rhizoma atractylodis macrocephalae, radix clematidis, fructus forsythiae, flos lonicerae, radix glycyrrhizae, fructus momordicae, semen ziziphi spinosae, fructus alpiniae oxyphyllae, semen boitae, bulbus lilii, flos rosae rugosae, tulip, paeonia suffruticosa, lavender, fructus gardeniae, jasmine flower, lilac, sweet-scented osmanthus, poria cocos, rhizoma dioscoreae, radix puerariae, radix polygoni multiflori, fructus cnidii, fructus corni, lycium chinense, longan, tortoise plastron, turtle shell, radix arnebiae, radix et rhizoma rhei, rhizoma alismatis, black nightshade, lotus flower, ginseng, lucid ganoderma, radix notoginseng, semen cassiae, herba lysimachiae, herba taraxaci, rhizoma seu radix notopterygii, medicated leaven, radix pseudostellariae and red ginseng. The composition is used for preparing the medicinal starter for brewing wine. The invention also provides a preparation method of the medicinal starter. The composition provided by the invention has a health-care function and a remarkable effect.