17 results about "Sugar transport" patented technology

The invention belongs to the technical field of plant genetic engineering, and relates to a tulip transcription factor TgTCP2 for regulating and controlling sugar transport and growth and development of plants and application of the tulip transcription factor TgTCP2, the plant growth and development traits comprise at least one of seedball size, crown breadth size, rosette leaf number and plant height, the nucleotide sequence of the TgTCP2 gene is shown as SEQ ID NO: 1, and the nucleotide sequence of the TgTCP2 gene is shown as SEQ ID NO: 2. Or the corresponding amino acid sequence is as shown in SEQ ID NO: 2. The result shows that the overexpression TgTCP2 promotes the growth and development of arabidopsis thaliana, which is mainly reflected in the increase of crown breadth, the increase of rosette leaf number and the increase of plant height; a VIGS technology is utilized to silence TgTCP2, and it is found that tulip seedball development is inhibited by silence of the gene, the sugar content in tulip seedballs is reduced, and it is indicated that TgTCP2 has the effect of regulating tulip seedball development and sugar transport. Application of the gene has important practical significance on regulation and control of plant sugar transport and growth and development.

The invention provides an application of a peach transport protein PpSWEET11 in regulating and controlling the hexose content of fruits. According to the invention, the function of the PpSWEET11 gene is cloned and verified, and through overexpression of the PpSWEET11 in peach fruits and tomatoes, the glucose and fructose levels in an overexpression group are obviously increased compared with those in a control group, which indicates that the PpSWEET11 has a wide sugar transport function. The invention not only expounds the effect of the PpSWEET11 in the aspect of sugar accumulation and provides a key molecular insight in the aspect of improving sugar accumulation of peaches and other economically important fruit crops, but also can be applied to nutritional quality improvement and germplasm innovation of peach fruits.

The invention discloses recombinant bacillus subtilis with high yield of surfactin as well as a construction method and application of the recombinant bacillus subtilis, and belongs to the technical field of genetic engineering. According to the invention, non-essential genes are determined through analysis and identification, a simplified engineering strain is obtained, and the highest fermentation yield reaches 11.2 g / L; then, a PTS part sugar phosphorylase gene obtained through screening is transferred into the simplified engineering strain, the metabolism efficiency of carbohydrates is improved, transportation and utilization of the carbohydrates are optimized, a fatty acid transmembrane transporter gene is further transferred, fatty acid uptake is assisted, synthesis of surfactin is facilitated, and the yield of the surfactin is improved. The combination of the two realizes optimization of synthesis of surfactin through a composite carbon source. Compared with a simplified engineering strain, the genetically engineered bacterium obtained by the invention has the advantages that the yield of surfactin is increased by 57.1%, the yield is increased by 2.3 times compared with that of an unsimplified engineering strain EX, and the final shake-flask fermentation yield can reach 17.6 g / L. As an industrial chassis cell of high-yield lipopeptide, the cell has a good industrial application prospect.

The invention discloses a double promoter sequence for balancing galP and glk gene expression and an application of the double promoter sequence in an L-tryptophan synthesis process. According to the promoter combination, double-gene expression vectors of Ptac-galP and Pj23119-glk are constructed, basic groups are designed and merged, so that the-35 to-10 regions of the promoters for regulating and controlling the two genes are subjected to random replacement of the basic groups, and a pair of promoter combinations with relatively good expression balance is obtained by combining fluorescence high-throughput screening. Compared with an initial promoter combination, the combination can remarkably improve the sugar transport efficiency of escherichia coli, so that the efficiency of producing L-tryptophan by PTS defective escherichia coli is improved. The technology has important social and economic values.

The invention relates to the technical field of gene engineering, in particular to application of a knockout SlABCB13 gene in increasing the soluble sugar content of tomato fruits. The function of the SlABCB13 gene is deleted by mediating gene editing, so that the content of soluble sugar (cane sugar, fructose and glucose) and the content of soluble solids in tomato fruits are remarkably increased, and the SlABCB13 participates in sugar transport of the tomato fruits. Meanwhile, the mutant does not influence related yield indexes such as plant height, stem diameter, fruit size, fruit setting number and single-plant fruit yield, and a new thought is provided for the problem that yield and quality are difficult to consider in existing tomato breeding.

The present invention provides a benzopyran-type flavonoid compound, its preparation method and application, and a pharmaceutical preparation, belonging to the field of pharmaceutical chemistry technology. The present invention provides a benzopyran-type flavonoid compound having a structure represented by Formula I or Formula II. The benzopyran-type flavonoid compound provided by the present invention targets members of the SWEET protein family in plants, selectively inhibiting the sugar transport activity of plant SWEET proteins while also exhibiting broad-spectrum antibacterial activity. These combined effects enhance the broad-spectrum disease resistance of plants. #imgabs0#

The invention discloses cane sugar transporter genes SoERDL6-7 and SoTST5 as well as related biological materials and application thereof, and belongs to the technical field of biology. The invention provides two DNA (deoxyribonucleic acid) fragments which are any one of the following DNA fragments: 1) nucleotide sequences of the DNA fragments are SEQ ID NO: 2 and SEQ ID NO: 4, and 2) a nucleotide sequence which has more than 90% of homology with the nucleotide sequence shown in 1) and has the same function. Experiments prove that when the sugarcane SoERDL6-7 or SoTST5 gene is expressed in sorghum, the content of sucrose, glucose and fructose of sorghum stalks can be remarkably increased.

This disclosure belongs to the field of plant genetic engineering and provides a method for cultivating high-yield crops. Specifically, it provides a method for cultivating yield-increasing genes and high-yield crops by mining and screening biological materials related to sugar transport proteins, and overexpressing the biological materials through homologous transgenesis and / or heterologous transgenesis to regulate plant traits. Compared with wild-type plants, the yield of transgenic plants is significantly improved.

The invention belongs to the crossing field of synthetic biology and fermentation engineering, and relates to a lactobacillus brevis carbon source screening method based on a whole genome metabolism model. The method comprises the following steps: firstly, carrying out whole genome sequencing on a target strain, and constructing an initial model by utilizing gapseq; a representative cheap carbon source is used for carrying out a'two-stage sequential correction 'experiment, sugar transport and energy metabolism are constrained and corrected by a specific growth rate, and then GAD branch flux is constrained and corrected cooperatively by a glutamic acid-GABA conversion rate and a substrate cost, so that the model precision is improved. And then simulating a plurality of carbon sources in batches in the correction model, screening an optimal combination with high biomass, high GABA and low cost, and performing closed-loop verification through high-throughput micro-feeding and blind test experiments. And finally, the yield of the GABA is increased through'starvation-pulse 'material supplementation and pH gradient optimization, and a rapid, low-cost and rational carbon source screening process from a genome to process amplification is realized.

The invention provides a gene related to efficient accumulation of sorbitol and fructose in litchi pulp, a molecular marker and application of the gene in litchi quality improvement, and belongs to the technical field of biology. The SNP molecular marker related to the content of fructose and sorbitol in litchi pulp is obtained based on GWAS analysis and screening. The gene is a monosaccharide transporter LcSTP2 (SL01G0038020) on a litchi chromosome 1, and the nucleotide sequence of the gene is as shown in SEQ ID NO. 1; the molecular marker is a non-synonymous SNP: 01-54229842-C: T which is located in a coding region of a gene capable of carrying out sugar transport on a litchi chromosome 1; the SNP molecular marker can be applied to breeding materials for detecting the content of fructose and sorbitol in litchi, is beneficial to rapid detection of breeding materials with high fructose and sorbitol content, shortens the breeding period and improves the breeding efficiency of high-quality varieties. The detection cost is low, and the detection result is high in accuracy.

The application discloses a corn sugar transport gene ZmSTP24 and application thereof. The nucleotide sequence of the corn ZmSTP24 gene is shown as SEQ ID NO. 1, and the amino acid sequence coded by the gene is shown as SEQ ID NO. 2. Through CRISPR / Cas9 technology, the corn ZmSTP24 gene is edited and its function is researched. Compared with a transgenic receptor, the ZmSTP24 transgenic strain has a significantly reduced grain sucrose content, starch content and grain size. It is shown that the ZmSTP24 gene cloned in the application positively regulates the accumulation of grain sugar and other carbohydrates, and influences corn filling and grain size.

The invention discloses a morchella sugar transporter, a nucleic acid molecule, a recombinant vector, a recombinant cell and application thereof, and relates to the technical field of edible mushroom disease control. The STP3 gene is separated from morchella esculenta, and after the STP3 gene is transformed into edible mushrooms, the activity of oxidoreductase of the edible mushrooms can be improved, so that the fungal disease resistance is improved. Therefore, the STP3 gene, the recombinant vector and the recombinant cell provided by the invention have the effect of improving the fungus disease resistance of the edible fungi, and have wide application prospects in prevention and treatment of fungus diseases of the edible fungi.

The invention provides an organic-inorganic fertilization method for arched shed watermelons, and belongs to the technical field of watermelon planting.The organic-inorganic fertilization method comprises the steps that firstly, before the watermelons are transplanted, biogas slurry fertilizer, powder organic fertilizer, biological bacterial fertilizer, diammonium phosphate and A-type ternary compound fertilizer are sequentially applied, and then base fertilizer is formed; after the watermelons are transplanted, topdressing is conducted according to the growth stage of the watermelons, and in the vine extending stage, YM type compound microbial fertilizer and B type ternary compound fertilizer are sequentially applied to promote vine growth and expand the leaf area; in the young fruit stage, a YM type compound microbial fertilizer, a K type compound microbial fertilizer and a B type ternary compound fertilizer are sequentially applied, the root system absorption efficiency is enhanced, and sugar transportation is promoted; in the fruit swelling period, a YM type compound microbial fertilizer, an N type compound microbial fertilizer and a B type ternary compound fertilizer are sequentially applied to delay leaf senescence and guarantee continuous photosynthesis of leaves; wherein the YM type compound microbial fertilizer, the K type compound microbial fertilizer and the N type compound microbial fertilizer are biogas slurry fertilizers added with functional bacteria.

The application discloses a plant growth regulator and application thereof in promoting growth and coloring of eggplants under high-temperature stress, and belongs to the technical field of plant growth regulators. Methyl jasmonate and rhamnolipid are combined as active components of the plant growth regulator, which can effectively relieve the growth inhibition of eggplants under high-temperature stress, and synergistically improve the anthocyanin content in the pericarp of eggplants, significantly improve the coloring effect of eggplant fruits under high-temperature stress, and improve the fruit quality. The application adds a synergistic component on the basis of the active components; the synergistic component comprises potassium dihydrogen phosphate, calcium chloride, zinc sulfate and boric acid, and can further strengthen the active components from the aspects of energy and osmotic regulation, calcium signal and membrane stability, antioxidant enzyme activity and photosynthetic protection, cell wall integrity and sugar transport, so as to synergistically improve the heat tolerance of plants, promote anthocyanin accumulation of fruits and improve the coloring performance of fruit surfaces.

The invention discloses a plant growth regulator and application thereof in promoting growth and coloring of eggplants under high temperature stress, and belongs to the technical field of plant growth regulators. The methyl jasmonate and the rhamnolipid are combined to serve as active components of the plant growth regulator, growth inhibition of high-temperature stress on eggplants can be effectively relieved, the content of anthocyanin in eggplant peels is synergistically increased, the coloring effect of eggplant fruits under the high-temperature stress condition is remarkably improved, and the fruit quality is improved. The synergistic component is added on the basis of the active component; the synergistic components comprise monopotassium phosphate, calcium chloride, zinc sulfate and boric acid, and can further reinforce the active components from the aspects of energy and osmotic regulation, calcium signal and membrane stabilization, antioxidant enzyme activity and photosynthetic protection, cell wall integrity, sugar transportation and the like respectively; therefore, plant heat resistance is synergistically improved, fruit anthocyanin accumulation is promoted, and fruit surface coloring performance is improved.

The invention discloses a gene participating in waxberry fruit sugar transport and application thereof, relates to the field of plant molecular biotechnology and genetic engineering, and provides a nucleotide sequence of the gene as shown in SEQ: NO.1, application of the gene in increasing sugar content in fruits and application of the gene in waxberry quality breeding. The functional research of the SWEET gene family in fruit quality regulation and control is enriched, a new theoretical basis is provided for a fruit sugar metabolism regulation and control network, and the gene has important application value in waxberry quality improvement breeding and provides a molecular basis for cultivating a new variety of high-quality waxberry.

The utility model belongs to the technical field of sugar alcohol production equipment, and particularly relates to a sugar transporting trolley for a sugar alcohol finished product workshop. Comprising a frame, a lifting machine is installed on the frame, a candy bucket is fixed to the lifting machine, a candy scraping motor is installed above the candy bucket, two scraping plates are welded to the output end of the candy scraping motor, the output end of the candy bucket is communicated with a socket piece, an inserting plate is inserted into the socket piece, a gear ring and a bearing are fixed to the outer wall of the output end of the candy bucket, and a mounting plate is fixed to the bearing. A gear motor is installed on the installation plate, a gear is fixed to the output end of the gear motor and meshed with the gear ring, a chute is fixed to the installation plate, and a centering pipe is welded to the bottom end of the chute. According to the utility model, discharging of the crystallizing tank and charging of the centrifugal machine can be completed by only one person, massecuite can be uniformly distributed, the working efficiency is improved, and eccentric operation of the centrifugal machine is prevented.