Rice sugar transport gene osmst1 and its sugar transporter, application and amplification primers
A sugar transfer and rice technology, applied in the introduction of foreign genetic material using vectors, applications, genetic engineering, etc., can solve the problems of high empty seedling rate, poor source-to-storage transportation, and insufficient grain filling, and achieve the goal of increasing yield. Effect
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Embodiment 1
[0036] A rice sugar transport gene OsMST1 , whose nucleotide sequence is shown in SEQ ID NO:1;
[0037] the sugar transporter gene OsMST1 The sugar transporter OsMST1 was discovered in rice through CRISPRE-CAS9 technology, as well as plant molecular genetics and physiological and biochemical methods. After research, we also found that its overexpression can significantly increase the yield of rice, and its gene OsMST1 The mutation of the gene significantly reduces the seed-setting rate and yield of rice, which provides a realistic basis for improving the transportation and distribution of photosynthetic products through genetic improvement of varieties, and continuously improving the yield of rice.
[0038] In order to verify the sugar transport gene of the present invention OsMST1and the function of its sugar transporter OsMST1, the following experiments were carried out:
[0039] 1. The sugar transport gene of the present invention OsMST1 The spatiotemporal expression p...
Embodiment 2
[0064] Rice sugar transport gene of the present invention OsMST1 The application of its sugar transporter (sugar transporter OsMST1) in cultivating high-yielding rice (increasing rice seed setting rate and yield per plant), specifically including OsMST1 Construction of overexpression and mutant plants, maturation phenotype and yield factor analysis.
[0065] In order to verify the sugar transport gene of the present invention OsMST1 In rice, we use CRISPR-Cas9 gene editing technology to OsMST1 Edited to obtain two knockout lines with different mutation types osmst1-1 , osmst1-2 ,Such as Figure 4 As shown, the knockout strain osmst1-1 The mutation type is 5 base deletions, knockout strains osmst1-2 The type of mutation is a deletion of 4 bases.
[0066] And for wild type (WT) and mutant ( osmst1-1 , osmst1-2 ) analysis of soluble sugar and starch content in each part during the grouting period, as follows:
[0067] 1. Extraction method of total soluble sugar: 1...
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