20 results about "Sugar transporter" patented technology

The invention discloses a cherry sugar transporter gene CpSWEET10 and application thereof, and belongs to the technical field of cherry gene engineering. A novel sugar transporter gene CpSWEET10 is obtained through cloning, the nucleotide sequence of the novel sugar transporter gene CpSWEET10 is shown as SEQ ID NO.1, and the coded amino acid sequence of the novel sugar transporter gene CpSWEET10 is shown as SEQ ID NO.2. The gene is highly expressed in cherry fruits, and the content of soluble solids and total sugar in mature fruits is remarkably positively correlated with the expression quantity of the soluble solids and the total sugar; through subcellular localization and sugar transport function defect type yeast mutant functional complementation experimental analysis, the protein coded by the CpSWEET10 gene is localized in a cytoplasmic membrane and has a sucrose transport function; the cherry sugar transporter gene CpSWEET10 disclosed by the invention can be applied to the improvement of the sugar degree character of the fruits, and a new gene resource is provided for molecular breeding for improving the high-sugar quality of the cherry fruits.

Described herein are methods of for treating and / or preventing Major adverse cardiovascular events (MACE). by administering to a subject in need thereof, a combination of a sodium-glucose transport protein 2 (SGLT2) inhibitor and a compound of Formula I or a stereoisomer, tautomer, pharmaceutically acceptable salt, or hydrate thereof, wherein the variables of Formula I are as defined herein.

The invention discloses selenoglycoside gligliflozin as well as a preparation method and application thereof. The structural formula of the selenoglycoside gligliflozin disclosed by the invention is shown as a formula (I). The preparation method of the seleno-glucoside gliflozin comprises the following steps: 1, directly carrying out selenium-carbon coupling reaction on peracetyl-protected 1-seleno-glucose in a solvent under the action of alkali and a palladium catalyst to prepare tetra-acetyl-protected seleno-glucoside gliflozin; and step 2, carrying out deacylation on the tetra-acetyl protected seleno-glucoside gliliflozin product to prepare the seleno-glucoside gliliflozin. A biological activity test shows that the selenoglycoside gliliflozin provided by the invention shows low toxicity to cells, high inhibitory activity to SGLT-2 (sodium glucose transporter 2) and beta-glucosidase hydrolysis resistance, so that the selenoglycoside gliliflozin has the potential to be developed into a medicine for treating diabetes mellitus 2. (I)

Disclosed herein are methods of treating and ameliorating hyperinsulin hypoglycemia using sodium dependent glucose transporter (SGLT) 1 inhibitor compounds and compositions comprising them.

The present invention provides methods and compositions for the prevention, reduction or treatment of adverse effects in a subject caused by a weight-losing drug containing an insulin agonist or antagonist or a sodium-glucose transporter 2 (SGLT-2) inhibitor, the subject is being treated with the weight-reducing drug or the SGLT-2 inhibitor, which contains the intestinal insulinotropic agonist or antagonist. The method comprises, in a subject receiving therapy with a drug or an SGLT-2 agent comprising an insulin agonist or antagonist, administering the SGLT-2 agent to the subject, co-administering to the subject, or upon disruption of the weight losing drug or SGLT-2 inhibitor containing the insulin agonist or antagonist, a therapeutically effective amount of a selective androgen receptor modulator (SARM) compound as disclosed herein represented by the structures of Formulae I, II, VIII, IX, X, XI, XII, XIII, and XIV.

Described herein are methods for treating and / or preventing a kidney disease or an associated disorder as measured by an increase in estimated glomerular filtration rate (eGFR) by administering to a subject in need thereof, a combination of a sodium-glucose transport protein 2 (SGLT2) inhibitor and a compound of Formula I or a stereoisomer, tautomer, pharmaceutically acceptable salt, or hydrate thereof, wherein the variables of Formula I are as defined herein.

The invention relates to application of trichocephalus microcoleus in production of GG and construction of engineered trichocephalus microcoleus with high yield of GG. According to the method disclosed by the invention, engineered coleus microcoleus with high yield of GG is constructed, and culture conditions for synthesizing glycerol glucoside by the coleus microcoleus BL0902 are optimized. Glycerin and glucose are added into the culture medium at the same time, so that the GG yield is improved to the optimal degree, and the GG yield is improved by about 4 times. Through overexpression of gtr, ggpS and ggpP, carbon flow is promoted to enter a path for synthesizing glycerol glucoside. The result shows that the total GG yield of the gtr-overexpressed algal strain is increased from 193.6 mg / L to about 337 mg / L 24 hours after the gtr-overexpressed algal strain is externally added with glycerol and glucose. On the basis, the ggpS and the ggpP are further overexpressed, and the total GG yield of the algae strain is increased to about 984.7 mg / L. In conclusion, the key genes ggpS and ggpP synthesized by overexpressing the glucose transporter gene gtr and GG, the GG yield is remarkably increased and can reach 984.7 mg / L to the maximum, and the yield is 393.88 mg / L / d.

This invention relates to the treatment of glucose transporter type 1 deficiency syndrome (Glut1-DS). In particular, the invention relates to specific medium-chain triglyceride compositions, enriched for C10 (decanoic acid), for use in treating movement disorders in Glut1-DS patients.

Provided herein are compositions, kits, and methods for manufacturing cells for treating or regenerating damaged or diseased tissue comprising engineered fibroblasts, myoblasts, osteocytes, chondrocytes, adipocytes, epithelial cells, or mesenchymal stem cells that overexpress glucose transporter 5 (GLUTS).

This disclosure belongs to the field of plant genetic engineering and provides a method for cultivating high-yield crops. Specifically, it provides a method for cultivating yield-increasing genes and high-yield crops by mining and screening biological materials related to sugar transport proteins, and overexpressing the biological materials through homologous transgenesis and / or heterologous transgenesis to regulate plant traits. Compared with wild-type plants, the yield of transgenic plants is significantly improved.

This invention discloses a sugar transporter gene. RkMAL1 Its nucleotide sequence is shown in SEQ ID NO:1, and the amino acid sequence encoded by this gene is shown in SEQ ID NO:2. This invention utilizes *Rhodotorula rubrum* (…). Rhodosporidium kratochvilovae Glycotransporter gene isolated from ) RkMAL1 uridine diphosphate glucose pyrophosphorylase RkUGPP After ligating the two components to a vector, they were transferred into *Rhodotorula rubrum* to achieve gene transfer. RkMAL1、 Gene RkMAL1+RkUGPP Overexpression of the gene significantly improves the synthesis efficiency of extracellular polysaccharides in recombinant strains. This invention provides a new method for producing extracellular polysaccharides, which is simple, easy to operate, and offers good application prospects for the large-scale production of extracellular polysaccharides.

The invention discloses a sugarcane sucrose transporter ScSUT4 and application thereof, and belongs to the technical field of biology. The application comprises any one of the following applications: C1) increasing the sugar content of the plant, C1) increasing the sugar content of the plant, C2) increasing the sugar content of the plant, C1) increasing the sugar content of the plant, and C2) increasing the sugar content of the plant; the method comprises the following steps of (1) preparing a product for increasing the sugar content of a plant, (2) preparing a product for increasing the sugar content of the plant, (3) cultivating the plant with the increased sugar content, (4) preparing a product for cultivating the plant with the increased sugar content, and (5) preparing a product for cultivating the plant with the increased sugar content, and the amino acid sequence of the protein is SEQ ID NO.1. Experiments prove that the sugar content in the sugarcane can be increased by reducing the relative expression quantity of the ScSUT4 gene in the sugarcane.

This invention relates to the field of agricultural technology, particularly rice. OsSWEET1b Application in increasing anthocyanin content in black rice grains; rice OsSWEET1b The use of genes or vectors to increase the anthocyanin content of black rice grains. OsSWEET1b The gene CDS is shown in Sequence 1 of the sequence listing; a method to increase the anthocyanin content of black rice grains, by... OsSWEET1b Gene overexpression; utilizing the OsSWEET1b gene to regulate anthocyanin synthesis and OsSWEET1b The gene, functioning as a plasma membrane-localized sugar transporter, can simultaneously achieve high anthocyanin content and stable yield, providing a key breakthrough for resolving the "nutritional quality-yield" contradiction in black rice; through overexpression... OsSWEET1b Genes, or their use in marker-assisted selection breeding, can significantly increase the anthocyanin content and yield of black rice grains, thus cultivating new black rice varieties that are "high in nutrition and high in yield".

IMPROVED GLUT1-BINDING POLYPEPTIDES The present invention relates to a glucose transporter protein type 1 (GLUT1)-binding polypeptide comprising, or consisting of, an amino acid sequence selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 22 to 26, and variants or fragments thereof, wherein the sequences of said variants or fragments comprise a serine (S) residue at position corresponding to position 95 of SEQ ID NO: 1, 23 and 25, or to position 97 of SEQ ID NO: 22, 24 and 26. The present invention further relates to in vitro and in vivo methods of specifically detecting or / and measuring the level of GLUT1, and to the use of said polypeptides in diagnosis and therapy.

Disclosed is a mutant, non-naturally occurring, or transgenic Nicotiana tabacum plant comprising at least one modification capable of modulating the expression or activity of one or more of SWEET12-S or SWEET12-T or SWEET15-S or SWEET15-T, wherein the plant or portion thereof comprises at least one modification capable of modulating (a) the expression of a polynucleotide in the plant or portion thereof, or (b) the activity of a polypeptide in the plant or portion thereof, compared to a control Nicotiana tabacum plant or portion thereof, and wherein expression or activity of one or more of SWEET12-S or SWEET12-T or SWEET15-S or SWEET15-T is modulated compared to the control Nicotiana tabacum plant.

The invention belongs to the technical field of agricultural biology, and particularly relates to a high-activity xylose transporter mutant C181G and application thereof. The xylose transporter mutant provided by the invention has higher xylose transport activity. Compared with a wild type, the xylose transport protein mutant C181G-overexpressed engineering strain of the mycelia thermophilus has the advantage that the xylose transport rate of the xylose transport protein mutant C181G-overexpressed engineering strain under a mixed sugar system is improved by 48%. The invention provides a key functional element for constructing a chassis cell for efficiently and jointly utilizing glucose and xylose in the straw hydrolysate, and has important application value and technical significance for promoting efficient biotransformation of lignocellulose biomass.

The application belongs to the field of plant molecular biology, and discloses a peach NAC class transcription factor PpNAC050A gene of peach pulp monosaccharide transporter PpERDL16 and application of the gene. The amino acid sequence of the protein encoded by the gene is shown as SEQ ID NO: 2. The application proves that the gene can be combined with the monosaccharide transporter PpERDL16 promoter, and through regulating the activity, the accumulation of fructose is affected. Further, the overexpression system of the gene is successfully constructed in the peach pulp, the activity of the monosaccharide transporter PpERDL16 can be effectively inhibited, the accumulation of fructose in the pulp is increased, and the sweetness of the pulp is increased. Therefore, the quality of the peach pulp can be improved by overexpressing the gene, important theoretical basis for cultivating high-quality fruits is provided, and the application has a wide application prospect.

Provided herein are compositions, kits, and methods for manufacturing cells for treating or regenerating damaged or diseased tissue comprising engineered fibroblasts, myoblasts, osteocytes, chondrocytes, adipocytes, epithelial cells, or mesenchymal stem cells that overexpress glucose transporter 5 (GLUTS).

The present invention relates to compositions and methods for improving metabolic health. Specifically, the present disclosure relates to using a methylated phloretin analog as a sodium-glucose transporter-2 (SGLT2) inhibitor for improving metabolic health.

The present application belongs to the field of microorganism and fermentation engineering technology, and particularly relates to a genetically engineered bacterium for producing 2'-fucosyllactose based on mannitol and a construction method and application thereof. Specifically, on the basis of the previously constructed 2'-fucosyllactose-producing engineered strain, the 2'-fucosyllactose synthesis pathway is iteratively integrated, the GDP-D-mannose synthesis pathway is enhanced, the hexose transporter Yht1 is overexpressed, the GDP-D-mannose synthesis pathway is further enhanced, and the hexose transporter Yht3 is knocked out, so that the Yarrowia lipolytica strain can efficiently absorb mannitol for growth metabolism and synthesis of 2'-fucosyllactose. The present application is the first report of using mannitol as a substrate for the production of 2'-fucosyllactose, and has important industrial application value.