47 results about "Sugar transporter" patented technology

The invention discloses a cherry sugar transporter gene CpSWEET10 and application thereof, and belongs to the technical field of cherry gene engineering. A novel sugar transporter gene CpSWEET10 is obtained through cloning, the nucleotide sequence of the novel sugar transporter gene CpSWEET10 is shown as SEQ ID NO.1, and the coded amino acid sequence of the novel sugar transporter gene CpSWEET10 is shown as SEQ ID NO.2. The gene is highly expressed in cherry fruits, and the content of soluble solids and total sugar in mature fruits is remarkably positively correlated with the expression quantity of the soluble solids and the total sugar; through subcellular localization and sugar transport function defect type yeast mutant functional complementation experimental analysis, the protein coded by the CpSWEET10 gene is localized in a cytoplasmic membrane and has a sucrose transport function; the cherry sugar transporter gene CpSWEET10 disclosed by the invention can be applied to the improvement of the sugar degree character of the fruits, and a new gene resource is provided for molecular breeding for improving the high-sugar quality of the cherry fruits.

Described herein are methods of for treating and / or preventing Major adverse cardiovascular events (MACE). by administering to a subject in need thereof, a combination of a sodium-glucose transport protein 2 (SGLT2) inhibitor and a compound of Formula I or a stereoisomer, tautomer, pharmaceutically acceptable salt, or hydrate thereof, wherein the variables of Formula I are as defined herein.

The invention provides an application of a peach transport protein PpSWEET11 in regulating and controlling the hexose content of fruits. According to the invention, the function of the PpSWEET11 gene is cloned and verified, and through overexpression of the PpSWEET11 in peach fruits and tomatoes, the glucose and fructose levels in an overexpression group are obviously increased compared with those in a control group, which indicates that the PpSWEET11 has a wide sugar transport function. The invention not only expounds the effect of the PpSWEET11 in the aspect of sugar accumulation and provides a key molecular insight in the aspect of improving sugar accumulation of peaches and other economically important fruit crops, but also can be applied to nutritional quality improvement and germplasm innovation of peach fruits.

The invention discloses selenoglycoside gligliflozin as well as a preparation method and application thereof. The structural formula of the selenoglycoside gligliflozin disclosed by the invention is shown as a formula (I). The preparation method of the seleno-glucoside gliflozin comprises the following steps: 1, directly carrying out selenium-carbon coupling reaction on peracetyl-protected 1-seleno-glucose in a solvent under the action of alkali and a palladium catalyst to prepare tetra-acetyl-protected seleno-glucoside gliflozin; and step 2, carrying out deacylation on the tetra-acetyl protected seleno-glucoside gliliflozin product to prepare the seleno-glucoside gliliflozin. A biological activity test shows that the selenoglycoside gliliflozin provided by the invention shows low toxicity to cells, high inhibitory activity to SGLT-2 (sodium glucose transporter 2) and beta-glucosidase hydrolysis resistance, so that the selenoglycoside gliliflozin has the potential to be developed into a medicine for treating diabetes mellitus 2. (I)

The invention relates to the technical field of pharmaceutical preparations, in particular to mRNA (messenger ribonucleic acid) lipid nanoparticles and application thereof in treatment of triple-negative breast cancer. The invention constructs an mRNA lipid nanoparticle, breaks through the dilemma of single drug curative effect of a glucose transporter (GLUT) inhibitor and a glutaminase (GLS) inhibitor for triple negative breast cancer, and realizes double-track treatment of mRNA metabolism reprogramming and small molecule energy deprivation for the first time. The mRNA lipid nanoparticles enhance the sensitivity of triple negative breast cancer to small molecule energy deprivation treatment, so that the clinical transformation feasibility of BAY-876 and CB-839 is improved to a new dimension. The delivery efficiency of the mRNA lipid nanoparticles is high, the biological safety is good, meanwhile, the production process of the nanoparticles is simpler and more standard compared with plasmids and viruses, and the drug production cost is lower.

Disclosed herein are methods of treating and ameliorating hyperinsulin hypoglycemia using sodium dependent glucose transporter (SGLT) 1 inhibitor compounds and compositions comprising them.

This invention belongs to the field of biological genes and discloses the application of a rice monosaccharide transporter gene OsPLT1 and its transporter in regulating rice growth period. The rice monosaccharide transporter gene... OsPLT1 The nucleotide sequence is shown in SEQ ID NO: 1. This invention also discloses a gene for a rice monosaccharide transporter. OsPLT1 Amplification primers. This invention provides rice. OsPLT1 Novel applications of genes and their encoded proteins in regulating rice growth stage; knockout of wild-type ZH11 using CRISPR / Cas9. OsPLT1 At this time, it can accelerate the growth process of rice without affecting the yield of rice, therefore, OsPLT1 Genes can be used to regulate plant growth periods and increase annual and seasonal yields, and have high economic and application value.

The invention discloses application of transporter to increase yield of beta-alanine and constructed probiotic Escherichia coli, rhamnose transporter RhaT capable of increasing the yield of beta-alanine is obtained through screening, and the shake-flask fermentation yield of beta-alanine reaches 1.85 g / L through overexpression of rhamnose transporter rhaT gene, so that the yield of beta-alanine is increased, the yield of beta-alanine is increased, and the yield of beta-alanine is increased. And the content of beta-alanine in the fermentation tank is 73.11 g / L. Compared with a control strain, the shake-flask fermentation yield of beta-alanine is improved by 7.56%. In the future, related expression genes of serine and threonine of competitive branches of beta-alanine can be inhibited or knocked out by combining a protein structure optimization or directed evolution strategy, so that the yield of beta-alanine is further increased.

The present invention relates to a key intermediate for preparing a glucopyranosyl derivative, which is a sodium-dependent glucose transporter (SGLT) inhibitor, and a preparation method thereof. Specifically, the key intermediate provided by the present invention has stable properties, few impurities, and high optical purity (diastereoselectivity). The preparation method provided by the present invention utilizes inexpensive reagents, mild reaction conditions, does not require silica gel column chromatography purification, is simple to post-process, is easy to purify, has low equipment requirements, low production costs, and a safe and controllable process, making it suitable for industrial production.

The present invention provides methods and compositions for the prevention, reduction or treatment of adverse effects in a subject caused by a weight-losing drug containing an insulin agonist or antagonist or a sodium-glucose transporter 2 (SGLT-2) inhibitor, the subject is being treated with the weight-reducing drug or the SGLT-2 inhibitor, which contains the intestinal insulinotropic agonist or antagonist. The method comprises, in a subject receiving therapy with a drug or an SGLT-2 agent comprising an insulin agonist or antagonist, administering the SGLT-2 agent to the subject, co-administering to the subject, or upon disruption of the weight losing drug or SGLT-2 inhibitor containing the insulin agonist or antagonist, a therapeutically effective amount of a selective androgen receptor modulator (SARM) compound as disclosed herein represented by the structures of Formulae I, II, VIII, IX, X, XI, XII, XIII, and XIV.

The present invention relates to a pharmaceutical composition containing a sodium-glucose cotransporter-2 inhibitor and an angiotensin II receptor blocker for the prevention or treatment of obesity. The pharmaceutical composition has no problem of interaction between drugs, shows a synergistic effect on weight loss, achieves rapid weight loss at the beginning of administration, and exhibits an effect of maintaining weight loss independent of dietary intake during long-term administration, and thus can be effectively applied to obese patients.

Described herein are methods for treating and / or preventing a kidney disease or an associated disorder as measured by an increase in estimated glomerular filtration rate (eGFR) by administering to a subject in need thereof, a combination of a sodium-glucose transport protein 2 (SGLT2) inhibitor and a compound of Formula I or a stereoisomer, tautomer, pharmaceutically acceptable salt, or hydrate thereof, wherein the variables of Formula I are as defined herein.

The invention relates to application of trichocephalus microcoleus in production of GG and construction of engineered trichocephalus microcoleus with high yield of GG. According to the method disclosed by the invention, engineered coleus microcoleus with high yield of GG is constructed, and culture conditions for synthesizing glycerol glucoside by the coleus microcoleus BL0902 are optimized. Glycerin and glucose are added into the culture medium at the same time, so that the GG yield is improved to the optimal degree, and the GG yield is improved by about 4 times. Through overexpression of gtr, ggpS and ggpP, carbon flow is promoted to enter a path for synthesizing glycerol glucoside. The result shows that the total GG yield of the gtr-overexpressed algal strain is increased from 193.6 mg / L to about 337 mg / L 24 hours after the gtr-overexpressed algal strain is externally added with glycerol and glucose. On the basis, the ggpS and the ggpP are further overexpressed, and the total GG yield of the algae strain is increased to about 984.7 mg / L. In conclusion, the key genes ggpS and ggpP synthesized by overexpressing the glucose transporter gene gtr and GG, the GG yield is remarkably increased and can reach 984.7 mg / L to the maximum, and the yield is 393.88 mg / L / d.

This invention relates to the treatment of glucose transporter type 1 deficiency syndrome (Glut1-DS). In particular, the invention relates to specific medium-chain triglyceride compositions, enriched for C10 (decanoic acid), for use in treating movement disorders in Glut1-DS patients.

The present invention belongs to the field of genetic engineering technology, specifically relating to genetically engineered bacteria and their use in xylitol production. The genetically engineered bacteria include a host cell and a xylose reductase gene and a xylose transporter gene inserted into the host cell. The present invention introduces the xylose transporter gene and the xylose reductase gene into Vibrio natriuresis. The resulting genetically engineered bacteria can functionally express and obtain a recombinant xylose transporter, thereby transporting extracellular xylose into the cell and converting the xylose into xylitol using the xylose reductase. This invention is the first to construct a genetically engineered Vibrio natriuresis bacterium capable of utilizing xylose and producing xylitol, which is of great significance for subsequent research on the use of Vibrio natriuresis in xylitol production.

The present disclosure relates to a combination therapy for treating cancer comprising belinostat or a pharmaceutically acceptable salt, tautomer, stereoisomer, mixture of stereoisomers, prodrug, or deuterated analog thereof and at least one glucose transporters (GLUT) inhibitor. Belinostat and the GLUT inhibitor can either be administered separately or in the form of a single composition. Thus, the disclosure relates to a kit comprising Belinostat and at least one GLUT inhibitor, wherein Belinostat and the GLUT inhibitor are in separate formulations and, alternatively, to a composition comprising belinostat and at least one GLUT inhibitor, such as a pharmaceutical composition. It also relates to such kit or such composition for use in the treatment of cancer, preferably for the treatment of lymphoma(s).

The invention discloses cane sugar transporter genes SoERDL6-7 and SoTST5 as well as related biological materials and application thereof, and belongs to the technical field of biology. The invention provides two DNA (deoxyribonucleic acid) fragments which are any one of the following DNA fragments: 1) nucleotide sequences of the DNA fragments are SEQ ID NO: 2 and SEQ ID NO: 4, and 2) a nucleotide sequence which has more than 90% of homology with the nucleotide sequence shown in 1) and has the same function. Experiments prove that when the sugarcane SoERDL6-7 or SoTST5 gene is expressed in sorghum, the content of sucrose, glucose and fructose of sorghum stalks can be remarkably increased.

The present invention addresses the problem of controlling a fusobacterium bacterium by means of a method which is believed to have little influence on beneficial bacteria. The present invention provides a composition for controlling bacteria belonging to the genus fusobacterium in the digestive tract, particularly in the oral and intestinal flora, comprising any one selected from the group consisting of flavonoids and sugar transporters thereof that dissociate a compound represented by formula I in the digestive tract, and the compounds represented by formula I. The composition can be used for controlling the bacteria belonging to the genus fusobacterium in the digestive tract, particularly in the oral and intestinal flora.

Provided herein are compositions, kits, and methods for manufacturing cells for treating or regenerating damaged or diseased tissue comprising engineered fibroblasts, myoblasts, osteocytes, chondrocytes, adipocytes, epithelial cells, or mesenchymal stem cells that overexpress glucose transporter 5 (GLUTS).

The invention belongs to the technical field of plant genetic engineering, and particularly discloses a peanut sugar transporter gene AhSWEET42 and application thereof. The nucleotide sequence of the gene AhSWEET42 is as shown in SEQ ID No. 1, and the amino acid sequence of the encoding protein of the gene AhSWEET42 is as shown in SEQ ID No. 2. An overexpression vector driven by a CaMV 35S promoter is constructed through a Gateway system, the overexpression vector is subjected to agrobacterium-mediated transformation of wild type arabidopsis thaliana Columbia-zero, molecular detection and soluble sugar content identification are carried out on a transgenic plant, and the soluble sugar content of the transgenic arabidopsis thaliana can be remarkably increased through overexpression of the overexpression vector in the arabidopsis thaliana; the result shows that AhSWEET42 may participate in plant sweet unloading and sugar accumulation reactions. The invention provides an important gene resource for cultivating new varieties such as high-sweetness peanuts by utilizing a genetic engineering means, and has an important application prospect.

This disclosure belongs to the field of plant genetic engineering and provides a method for cultivating high-yield crops. Specifically, it provides a method for cultivating yield-increasing genes and high-yield crops by mining and screening biological materials related to sugar transport proteins, and overexpressing the biological materials through homologous transgenesis and / or heterologous transgenesis to regulate plant traits. Compared with wild-type plants, the yield of transgenic plants is significantly improved.

This invention discloses a sugar transporter gene. RkMAL1 Its nucleotide sequence is shown in SEQ ID NO:1, and the amino acid sequence encoded by this gene is shown in SEQ ID NO:2. This invention utilizes *Rhodotorula rubrum* (…). Rhodosporidium kratochvilovae Glycotransporter gene isolated from ) RkMAL1 uridine diphosphate glucose pyrophosphorylase RkUGPP After ligating the two components to a vector, they were transferred into *Rhodotorula rubrum* to achieve gene transfer. RkMAL1、 Gene RkMAL1+RkUGPP Overexpression of the gene significantly improves the synthesis efficiency of extracellular polysaccharides in recombinant strains. This invention provides a new method for producing extracellular polysaccharides, which is simple, easy to operate, and offers good application prospects for the large-scale production of extracellular polysaccharides.

The invention discloses a sugarcane sucrose transporter ScSUT4 and application thereof, and belongs to the technical field of biology. The application comprises any one of the following applications: C1) increasing the sugar content of the plant, C1) increasing the sugar content of the plant, C2) increasing the sugar content of the plant, C1) increasing the sugar content of the plant, and C2) increasing the sugar content of the plant; the method comprises the following steps of (1) preparing a product for increasing the sugar content of a plant, (2) preparing a product for increasing the sugar content of the plant, (3) cultivating the plant with the increased sugar content, (4) preparing a product for cultivating the plant with the increased sugar content, and (5) preparing a product for cultivating the plant with the increased sugar content, and the amino acid sequence of the protein is SEQ ID NO.1. Experiments prove that the sugar content in the sugarcane can be increased by reducing the relative expression quantity of the ScSUT4 gene in the sugarcane.

The application discloses a method for improving heat tolerance and / or fruit setting rate of tomato pollen, and belongs to the technical field of biotechnology.The method is to improve heat tolerance and / or fruit setting rate of tomato pollen by overexpressing sugar transporter protein STP2 gene in the tomato, wherein the nucleotide sequence of the protein coding region of the STP2 gene is shown as SEQ ID NO.1, and the amino acid sequence of the protein coded by the STP2 gene is shown as SEQ ID NO.2.The application utilizes transgenic technology and tissue culture technology to screen and obtain homozygous lines OE:STP2-1 and OE:STP2-2 of the overexpressed STP2 gene.Compared with wild type WT, the overexpressed plant has significantly reduced pollen abnormality rate and significantly improved pollen germination rate under high temperature, and the fruit setting rate of the tomato under high temperature stress is also significantly improved, thereby providing important gene resources for cultivating tomato varieties resistant to high temperature.

The present disclosure provides cells that are competitive with respect to cancer cells, without experiencing starvation or exhaustion, even in glucose-depleted environments. Specifically, the present disclosure provides survivor T cells with enhanced glucose uptake capacity. The T cells are modified so as to express a glucose transporter and / or configured such that the expression of a glucose transporter is enhanced. Also provided are a cell population including such cells, a pharmaceutical composition, and a treatment method.

The invention belongs to the technical field of plant genetic engineering, and particularly discloses a peanut sugar transporter gene AhSUT4 and application thereof in improving taste and quality of peanuts. The nucleotide sequence of the gene AhSUT4 is as shown in SEQ ID No. 1, and the amino acid sequence of the encoding protein of the gene AhSUT4 is as shown in SEQ ID No. 2. An overexpression vector driven by a CaMV 35S promoter is constructed through a Gateway system, the overexpression vector is subjected to agrobacterium-mediated transformation of wild type arabidopsis thaliana Columbia-zero, molecular detection and soluble sugar content identification are carried out on a transgenic plant, and the soluble sugar content of the transgenic arabidopsis thaliana can be remarkably increased through overexpression of the overexpression vector in the arabidopsis thaliana; the result shows that AhSUT4 may participate in plant sweet unloading and sugar accumulation reactions. The invention provides an important gene resource for cultivating new varieties such as high-sweetness peanuts by utilizing a genetic engineering means, and has an important application prospect.

The present application relates to the field of agricultural biotechnology, and in particular to a xylose transport protein mutant with improved xylose transport capacity, and genes and applications thereof. Compared with the wild type, after overexpression of xylose transport protein Xyl, the utilization rate of xylose by the engineered strain of Myceliopthora thermophila reached 29%; after overexpression of the xylose transport protein mutants M365F, M365L, M365Y, M365S and M365V, the utilization rate of xylose by the engineered strain of Myceliopthora thermophila reached 78%, 71%, 64%, 45% and 41% respectively. The engineered strain of Myceliopthora thermophila with overexpression of the xylose transport protein mutants M365F, M365L, M365Y, M365S and M365V had an increase of 163%, 140%, 119%, 53% and 38% in the transport of xylose under a mixed sugar system.

The invention belongs to the technical field of genetic engineering, and particularly relates to a genetically engineered bacterium and application thereof in xylitol production, and the genetically engineered bacterium comprises a host cell, and a xylose reductase gene and a xylose transporter gene which are inserted into the host cell. According to the invention, a xylose transporter gene and a xylose reductase gene are introduced into vibrio natriureticus, and the obtained genetically engineered bacterium can be functionally expressed to obtain a recombinant xylose transporter, so that extracellular xylose is transported into cells, and xylose is converted into xylitol by using xylose reductase. According to the invention, the vibrio natriticus genetically engineered bacterium capable of utilizing xylose and producing xylitol is constructed for the first time, and the vibrio natriticus genetically engineered bacterium is of great significance to subsequent research on production of xylitol by using vibrio natriticus.

The present application belongs to the technical field of sugar transporter activity detection, and particularly relates to a method for detecting the transport activity of vacuole membrane sugar transporter. The method comprises the following steps: constructing a plant expression vector for expressing the vacuole membrane sugar transporter, transforming the expression vector into the fruit of a melon at the swelling stage to obtain transgenic melon fruit; culturing the transgenic melon fruit until the vacuole membrane sugar transporter is expressed, and then obtaining protoplasts by enzymolysis; adding the protoplasts into a sugar fluorescent analogue culture solution, incubating for a period of time, and then detecting the fluorescence signal of the vacuoles in the protoplasts, and evaluating the transport activity of the vacuole membrane sugar transporter according to the fluorescence intensity. The method of the present application is simple to operate, high in efficiency, independent of isotopes, and capable of detecting the transport activity of both endogenous and exogenous vacuole sugar transporters, while maintaining the characteristics of the plant vacuole itself, reducing the interference of the background signal in the vacuole, and improving the accuracy and sensitivity of the analysis of the transport activity of the plant vacuole membrane sugar transporter.

This invention relates to the field of agricultural technology, particularly rice. OsSWEET1b Application in increasing anthocyanin content in black rice grains; rice OsSWEET1b The use of genes or vectors to increase the anthocyanin content of black rice grains. OsSWEET1b The gene CDS is shown in Sequence 1 of the sequence listing; a method to increase the anthocyanin content of black rice grains, by... OsSWEET1b Gene overexpression; utilizing the OsSWEET1b gene to regulate anthocyanin synthesis and OsSWEET1b The gene, functioning as a plasma membrane-localized sugar transporter, can simultaneously achieve high anthocyanin content and stable yield, providing a key breakthrough for resolving the "nutritional quality-yield" contradiction in black rice; through overexpression... OsSWEET1b Genes, or their use in marker-assisted selection breeding, can significantly increase the anthocyanin content and yield of black rice grains, thus cultivating new black rice varieties that are "high in nutrition and high in yield".