64 results about "Sugar transporter" patented technology

The invention relates to the technical field of agricultural biology, in particular to a myceliophthora thermophila genetic engineering strain with improved mixed sugar utilization capacity and application of the myceliophthora thermophila genetic engineering strain. The strain constitutively expresses the sugar transporter MtSTP of the mycelia thermophila, and the xylose utilization rate of the strain in a mixed sugar system is increased by 3.97 times compared with that of an original strain; the invention also relates to application of the strain with improved mixed sugar utilization capability in production of malic acid by fermentation of mixed sugar, and the malic acid yield of the strain in a mixed sugar system is improved by 0.63 times compared with that of an original strain. The method disclosed by the invention has important reference and guiding significance for construction of the chassis cells for efficiently utilizing the mixed sugar, and has important value for reducing the conversion cost of the lignocellulose biomass.

The invention discloses a cherry sugar transporter gene CpSWEET10 and application thereof, and belongs to the technical field of cherry gene engineering. A novel sugar transporter gene CpSWEET10 is obtained through cloning, the nucleotide sequence of the novel sugar transporter gene CpSWEET10 is shown as SEQ ID NO.1, and the coded amino acid sequence of the novel sugar transporter gene CpSWEET10 is shown as SEQ ID NO.2. The gene is highly expressed in cherry fruits, and the content of soluble solids and total sugar in mature fruits is remarkably positively correlated with the expression quantity of the soluble solids and the total sugar; through subcellular localization and sugar transport function defect type yeast mutant functional complementation experimental analysis, the protein coded by the CpSWEET10 gene is localized in a cytoplasmic membrane and has a sucrose transport function; the cherry sugar transporter gene CpSWEET10 disclosed by the invention can be applied to the improvement of the sugar degree character of the fruits, and a new gene resource is provided for molecular breeding for improving the high-sugar quality of the cherry fruits.

Described herein are methods of for treating and / or preventing Major adverse cardiovascular events (MACE). by administering to a subject in need thereof, a combination of a sodium-glucose transport protein 2 (SGLT2) inhibitor and a compound of Formula I or a stereoisomer, tautomer, pharmaceutically acceptable salt, or hydrate thereof, wherein the variables of Formula I are as defined herein.

The invention provides an application of a peach transport protein PpSWEET11 in regulating and controlling the hexose content of fruits. According to the invention, the function of the PpSWEET11 gene is cloned and verified, and through overexpression of the PpSWEET11 in peach fruits and tomatoes, the glucose and fructose levels in an overexpression group are obviously increased compared with those in a control group, which indicates that the PpSWEET11 has a wide sugar transport function. The invention not only expounds the effect of the PpSWEET11 in the aspect of sugar accumulation and provides a key molecular insight in the aspect of improving sugar accumulation of peaches and other economically important fruit crops, but also can be applied to nutritional quality improvement and germplasm innovation of peach fruits.

Described are compositions and methods relating to the over-expression of sugar transporter-like polypeptides to reduce the amount of glycerol and acetate produced by modified yeast having an exogenous pathway that cause it to produce more ethanol and acetate than its parental yeast.

The invention discloses a dsRNA nano preparation for preventing and controlling phytophthora capsici as well as a preparation method and application of the dsRNA nano preparation. The nucleotide sequence of the phytophthora capsici sugar transporter protein HXT4 gene is as shown in SEQ ID NO.1, and the ASPN-dsRNA nano preparation is obtained by mixing dsRNA as shown in SEQ ID NO.2 and amino-modified mesoporous silica. By exogenous application of the ASPN-dsRNA nano preparation, gene expression silencing of the target gene phytophthora capsici sugar transporter HXT4 is induced, so that pathogenicity of phytophthora capsici is reduced or even lost, and the purpose of protecting crops is achieved. The preparation can effectively reduce the pathogenicity of phytophthora capsici, the inhibition rate on phytophthora capsici infection can reach 72%, and the prevention and control efficiency on phytophthora capsici reaches 87%. The ASPN-dsRNA nano preparation is high in stability and obvious in prevention and control effect, and provides a new prevention and control strategy and means for developing a precise prevention and control method for crop epidemic diseases.

The invention discloses selenoglycoside gligliflozin as well as a preparation method and application thereof. The structural formula of the selenoglycoside gligliflozin disclosed by the invention is shown as a formula (I). The preparation method of the seleno-glucoside gliflozin comprises the following steps: 1, directly carrying out selenium-carbon coupling reaction on peracetyl-protected 1-seleno-glucose in a solvent under the action of alkali and a palladium catalyst to prepare tetra-acetyl-protected seleno-glucoside gliflozin; and step 2, carrying out deacylation on the tetra-acetyl protected seleno-glucoside gliliflozin product to prepare the seleno-glucoside gliliflozin. A biological activity test shows that the selenoglycoside gliliflozin provided by the invention shows low toxicity to cells, high inhibitory activity to SGLT-2 (sodium glucose transporter 2) and beta-glucosidase hydrolysis resistance, so that the selenoglycoside gliliflozin has the potential to be developed into a medicine for treating diabetes mellitus 2. (I)

Described herein are methods for treating and / or preventing a kidney disease or an associated disorder as measured by an increase in estimated glomerular filtration rate (eGFR) by administering to a subject in need thereof, a combination of a sodium-glucose transport protein 2 (SGLT2) inhibitor and a compound of Formula I or a stereoisomer, tautomer, pharmaceutically acceptable salt, or hydrate thereof, wherein the variables of Formula I are as defined herein.

The invention relates to the technical field of pharmaceutical preparations, in particular to mRNA (messenger ribonucleic acid) lipid nanoparticles and application thereof in treatment of triple-negative breast cancer. The invention constructs an mRNA lipid nanoparticle, breaks through the dilemma of single drug curative effect of a glucose transporter (GLUT) inhibitor and a glutaminase (GLS) inhibitor for triple negative breast cancer, and realizes double-track treatment of mRNA metabolism reprogramming and small molecule energy deprivation for the first time. The mRNA lipid nanoparticles enhance the sensitivity of triple negative breast cancer to small molecule energy deprivation treatment, so that the clinical transformation feasibility of BAY-876 and CB-839 is improved to a new dimension. The delivery efficiency of the mRNA lipid nanoparticles is high, the biological safety is good, meanwhile, the production process of the nanoparticles is simpler and more standard compared with plasmids and viruses, and the drug production cost is lower.

Disclosed herein are methods of treating and ameliorating hyperinsulin hypoglycemia using sodium dependent glucose transporter (SGLT) 1 inhibitor compounds and compositions comprising them.

This invention belongs to the field of biological genes and discloses the application of a rice monosaccharide transporter gene OsPLT1 and its transporter in regulating rice growth period. The rice monosaccharide transporter gene... OsPLT1 The nucleotide sequence is shown in SEQ ID NO: 1. This invention also discloses a gene for a rice monosaccharide transporter. OsPLT1 Amplification primers. This invention provides rice. OsPLT1 Novel applications of genes and their encoded proteins in regulating rice growth stage; knockout of wild-type ZH11 using CRISPR / Cas9. OsPLT1 At this time, it can accelerate the growth process of rice without affecting the yield of rice, therefore, OsPLT1 Genes can be used to regulate plant growth periods and increase annual and seasonal yields, and have high economic and application value.

The invention belongs to the technical field of microorganisms and fermentation engineering, and particularly relates to a genetically engineered bacterium for producing 2 '-fucosyllactose based on mannitol as well as a construction method and application of the genetically engineered bacterium. Specifically, on the basis of an engineering strain for producing 2 '-fucosyllactose, which is constructed in an earlier stage, 2'-fucosyllactose is produced; through iterative integration of a 2 '-fucosyllactose synthesis pathway, enhancement of a GDP-D-mannose synthesis pathway, overexpression of hexose transporter protein Yht1, further enhancement of the GDP-D-mannose synthesis pathway and knockout of hexose transporter protein Yht3, the Yarrowia lipolytica strain can efficiently absorb mannitol for growth metabolism and synthesize the 2'-fucosyllactose. According to the invention, the mannitol is firstly used as a substrate for production of the 2 '-fucosyllactose, and the mannitol has important industrial application value.

The invention discloses application of transporter to increase yield of beta-alanine and constructed probiotic Escherichia coli, rhamnose transporter RhaT capable of increasing the yield of beta-alanine is obtained through screening, and the shake-flask fermentation yield of beta-alanine reaches 1.85 g / L through overexpression of rhamnose transporter rhaT gene, so that the yield of beta-alanine is increased, the yield of beta-alanine is increased, and the yield of beta-alanine is increased. And the content of beta-alanine in the fermentation tank is 73.11 g / L. Compared with a control strain, the shake-flask fermentation yield of beta-alanine is improved by 7.56%. In the future, related expression genes of serine and threonine of competitive branches of beta-alanine can be inhibited or knocked out by combining a protein structure optimization or directed evolution strategy, so that the yield of beta-alanine is further increased.

The present invention relates to a key intermediate for preparing a glucopyranosyl derivative, which is a sodium-dependent glucose transporter (SGLT) inhibitor, and a preparation method thereof. Specifically, the key intermediate provided by the present invention has stable properties, few impurities, and high optical purity (diastereoselectivity). The preparation method provided by the present invention utilizes inexpensive reagents, mild reaction conditions, does not require silica gel column chromatography purification, is simple to post-process, is easy to purify, has low equipment requirements, low production costs, and a safe and controllable process, making it suitable for industrial production.

The present invention provides methods and compositions for the prevention, reduction or treatment of adverse effects in a subject caused by a weight-losing drug containing an insulin agonist or antagonist or a sodium-glucose transporter 2 (SGLT-2) inhibitor, the subject is being treated with the weight-reducing drug or the SGLT-2 inhibitor, which contains the intestinal insulinotropic agonist or antagonist. The method comprises, in a subject receiving therapy with a drug or an SGLT-2 agent comprising an insulin agonist or antagonist, administering the SGLT-2 agent to the subject, co-administering to the subject, or upon disruption of the weight losing drug or SGLT-2 inhibitor containing the insulin agonist or antagonist, a therapeutically effective amount of a selective androgen receptor modulator (SARM) compound as disclosed herein represented by the structures of Formulae I, II, VIII, IX, X, XI, XII, XIII, and XIV.

The invention belongs to the technical field of activity detection of sugar transporters, and particularly relates to a method for detecting the transport activity of a vacuolar sugar transporter. The method comprises the following steps: constructing a plant expression vector for expressing a vacuolar sugar transporter, and transforming a muskmelon fruit in a swelling period to obtain a transgenic muskmelon fruit; culturing the transgenic muskmelon fruit until the vacuolar sugar transporter protein is expressed, and then performing enzymolysis to obtain protoplast; adding the protoplast into a sugar fluorescence analogue culture solution, incubating for a period of time, then detecting a fluorescence signal of vacuoles in the protoplast, and evaluating the transport activity of the vacuole sugar transporter according to the fluorescence intensity. The method disclosed by the invention is simple and convenient to operate, high in efficiency and independent of isotope, has the characteristics that the transport activity of endogenous and exogenous vacuolar sugar transporters can be detected, also keeps the characteristics of the plant vacuoles, can reduce the interference of background signals in the vacuoles, and improves the accuracy and sensitivity of the analysis of the transport activity of the plant vacuolar sugar transporters.

The invention discloses a simple and efficient method for preparing gliflozin thioglycoside. Gliflozin thioglycoside is an SGLT2 (sodium glucose transporter 2) inhibitor with a novel molecular structure and a potential medicine for treating type 2 diabetes mellitus. The structural general formula of the gliflozin thioglycoside is as follows: # imgabs0 #. The preparation method of the gliflozin thioglycoside comprises the following two steps: 1, directly carrying out sulfur-carbon coupling reaction on 1-glucosinolate protected by peracetyl in a solvent under the action of alkali and a palladium catalyst to prepare a tetra-acetyl protected gliflozin thioglycoside product; and step 2, carrying out deacetylation on the tetra-acetyl protected gliflozin thioglycoside to prepare the gliflozin thioglycoside. The method is mild in reaction condition, simple and convenient to operate, relatively green and high in yield.

The present invention relates to a pharmaceutical composition containing a sodium-glucose cotransporter-2 inhibitor and an angiotensin II receptor blocker for the prevention or treatment of obesity. The pharmaceutical composition has no problem of interaction between drugs, shows a synergistic effect on weight loss, achieves rapid weight loss at the beginning of administration, and exhibits an effect of maintaining weight loss independent of dietary intake during long-term administration, and thus can be effectively applied to obese patients.

Described herein are methods for treating and / or preventing a kidney disease or an associated disorder as measured by an increase in estimated glomerular filtration rate (eGFR) by administering to a subject in need thereof, a combination of a sodium-glucose transport protein 2 (SGLT2) inhibitor and a compound of Formula I or a stereoisomer, tautomer, pharmaceutically acceptable salt, or hydrate thereof, wherein the variables of Formula I are as defined herein.

The invention discloses a method for improving tomato pollen heat resistance and / or high-temperature fruit setting rate, and belongs to the technical field of biology, the tomato pollen heat resistance and / or high-temperature fruit setting rate are / is improved through overexpression of a sugar transporter STP2 gene in tomatoes, the nucleotide sequence of a protein coding region of the STP2 gene is as shown in SEQ ID NO.1, and the nucleotide sequence of the protein coding region of the STP2 gene is as shown in SEQ ID NO.2. The amino acid sequence of the protein coded by the STP2 gene is as shown in SEQ ID NO. 2. According to the invention, STP2 gene overexpression homozygous strains OE: STP2-1 and OE: STP2-2 are screened by using a transgenic technology and a tissue culture technology, compared with wild type WT, the overexpression plants have the advantages that the pollen malformation rate at high temperature is obviously reduced, the germination rate is obviously increased, the tomato fruit setting rate at high temperature stress is also obviously increased, and the tomato yield is increased. An important gene resource is provided for cultivating a high-temperature-resistant tomato variety.

The invention relates to application of trichocephalus microcoleus in production of GG and construction of engineered trichocephalus microcoleus with high yield of GG. According to the method disclosed by the invention, engineered coleus microcoleus with high yield of GG is constructed, and culture conditions for synthesizing glycerol glucoside by the coleus microcoleus BL0902 are optimized. Glycerin and glucose are added into the culture medium at the same time, so that the GG yield is improved to the optimal degree, and the GG yield is improved by about 4 times. Through overexpression of gtr, ggpS and ggpP, carbon flow is promoted to enter a path for synthesizing glycerol glucoside. The result shows that the total GG yield of the gtr-overexpressed algal strain is increased from 193.6 mg / L to about 337 mg / L 24 hours after the gtr-overexpressed algal strain is externally added with glycerol and glucose. On the basis, the ggpS and the ggpP are further overexpressed, and the total GG yield of the algae strain is increased to about 984.7 mg / L. In conclusion, the key genes ggpS and ggpP synthesized by overexpressing the glucose transporter gene gtr and GG, the GG yield is remarkably increased and can reach 984.7 mg / L to the maximum, and the yield is 393.88 mg / L / d.

This invention relates to the treatment of glucose transporter type 1 deficiency syndrome (Glut1-DS). In particular, the invention relates to specific medium-chain triglyceride compositions, enriched for C10 (decanoic acid), for use in treating movement disorders in Glut1-DS patients.

The present invention belongs to the field of genetic engineering technology, specifically relating to genetically engineered bacteria and their use in xylitol production. The genetically engineered bacteria include a host cell and a xylose reductase gene and a xylose transporter gene inserted into the host cell. The present invention introduces the xylose transporter gene and the xylose reductase gene into Vibrio natriuresis. The resulting genetically engineered bacteria can functionally express and obtain a recombinant xylose transporter, thereby transporting extracellular xylose into the cell and converting the xylose into xylitol using the xylose reductase. This invention is the first to construct a genetically engineered Vibrio natriuresis bacterium capable of utilizing xylose and producing xylitol, which is of great significance for subsequent research on the use of Vibrio natriuresis in xylitol production.

The present disclosure relates to a combination therapy for treating cancer comprising belinostat or a pharmaceutically acceptable salt, tautomer, stereoisomer, mixture of stereoisomers, prodrug, or deuterated analog thereof and at least one glucose transporters (GLUT) inhibitor. Belinostat and the GLUT inhibitor can either be administered separately or in the form of a single composition. Thus, the disclosure relates to a kit comprising Belinostat and at least one GLUT inhibitor, wherein Belinostat and the GLUT inhibitor are in separate formulations and, alternatively, to a composition comprising belinostat and at least one GLUT inhibitor, such as a pharmaceutical composition. It also relates to such kit or such composition for use in the treatment of cancer, preferably for the treatment of lymphoma(s).

The invention discloses cane sugar transporter genes SoERDL6-7 and SoTST5 as well as related biological materials and application thereof, and belongs to the technical field of biology. The invention provides two DNA (deoxyribonucleic acid) fragments which are any one of the following DNA fragments: 1) nucleotide sequences of the DNA fragments are SEQ ID NO: 2 and SEQ ID NO: 4, and 2) a nucleotide sequence which has more than 90% of homology with the nucleotide sequence shown in 1) and has the same function. Experiments prove that when the sugarcane SoERDL6-7 or SoTST5 gene is expressed in sorghum, the content of sucrose, glucose and fructose of sorghum stalks can be remarkably increased.

Genetically modified yeast having a heterologous sugar transporter that is capable of transporting a non-glucose sugar such as maltulose, are described. The heterologous sugar transporter can be a protein according to, or that has similarity to, SEQ ID NO: 44. Fermentation methods using enzymatically treated starch where the yeast are able to consume the non-glucose sugars, are also described. The engineered yeast can be useful for producing desired bioproducts such as high ethanol, with low amounts of residual sugars in the medium.

Lipogenic yeasts bioengineered to overexpress genes for lipid production, and methods of use thereof. The yeasts are modified to express, constitutively express, or overexpress an acetyl- CoA carboxylase, an alpha-amylase, an ATP citrate lyase, a diacylglycerol acyltransferase, a fatty acid synthase, a glycerol kinase, a 6-phosphogluconate dehydrogenase, a glycerol-3- phosphate dehydrogenase, a malic enzyme, a fatty acyl-CoA reductase, a delta-9 acyl-CoA desaturase, a glycerol-3-phosphate acyltransferase, a lysophosphatidate acyltransferase, a glucose-6-phosphate dehydrogenase, a beta-glucosidase, a hexose transporter, a glycerol transporter, a glycoside hydrolase enzyme, an auxiliary activity family 9 enzyme, or combinations thereof. The yeasts in some cases are also modified to reduce or ablate activity of certain proteins. The methods include cultivating the yeast to convert low value soluble organic stillage byproducts into lipids suitable for biodiesel production and other higher value uses.

The present invention addresses the problem of controlling a fusobacterium bacterium by means of a method which is believed to have little influence on beneficial bacteria. The present invention provides a composition for controlling bacteria belonging to the genus fusobacterium in the digestive tract, particularly in the oral and intestinal flora, comprising any one selected from the group consisting of flavonoids and sugar transporters thereof that dissociate a compound represented by formula I in the digestive tract, and the compounds represented by formula I. The composition can be used for controlling the bacteria belonging to the genus fusobacterium in the digestive tract, particularly in the oral and intestinal flora.

Provided herein are compositions, kits, and methods for manufacturing cells for treating or regenerating damaged or diseased tissue comprising engineered fibroblasts, myoblasts, osteocytes, chondrocytes, adipocytes, epithelial cells, or mesenchymal stem cells that overexpress glucose transporter 5 (GLUTS).

The invention belongs to the technical field of plant genetic engineering, and particularly discloses a peanut sugar transporter gene AhSWEET42 and application thereof. The nucleotide sequence of the gene AhSWEET42 is as shown in SEQ ID No. 1, and the amino acid sequence of the encoding protein of the gene AhSWEET42 is as shown in SEQ ID No. 2. An overexpression vector driven by a CaMV 35S promoter is constructed through a Gateway system, the overexpression vector is subjected to agrobacterium-mediated transformation of wild type arabidopsis thaliana Columbia-zero, molecular detection and soluble sugar content identification are carried out on a transgenic plant, and the soluble sugar content of the transgenic arabidopsis thaliana can be remarkably increased through overexpression of the overexpression vector in the arabidopsis thaliana; the result shows that AhSWEET42 may participate in plant sweet unloading and sugar accumulation reactions. The invention provides an important gene resource for cultivating new varieties such as high-sweetness peanuts by utilizing a genetic engineering means, and has an important application prospect.