299 results about "Arbuscular mycorrhiza" patented technology

The invention discloses a functional microbial fertilizer and a production method thereof. The production method comprises the following steps: grinding 60%-70% of mushroom residue and 15%-25% of livestock manure to 2-3mm to obtain the major material by a grinder, regulating water content to be 60%-65%; adding composite fermenting agent consisting of bacillus subtilis, bacillus licheniformis, trichoderma harzianum and trichoderma viride in accordance with 50g-100g/T; stacking, fermenting and maturing for 15-30d, and turning 5-10 times during the process; evenly mixing the major material prepared from fermentation with auxiliary materials consisting of 6%-10% humic acid and 6%-10% rice hull; adding arbuscular mycorrhiza agent in accordance with 50g-100g/T; and sieving with a 3mm sieve to obtain the fertilizer. The invention provides an economic and effective technological approach for the resource recycling of agricultural wastes in China, and has important application prospect on promoting the development of green agriculture, controlling and reducing rural environmental pollution, improving rural eco-environment and other aspects.

The invention discloses a microbial fertilizer producing by using agricultural wastes and preparation thereof. Main materials are obtained by mixing and smashing 20%-40% oil sally bean dregs, 40%-60% crop straw and 15%-25% poultry excrement, moisture content achieves 60-65%, composite leaven is added according to 150g-200g/T to pile to be cone-shaped, yellow mud is used on the outside for sealing, a plastic film is used for covering in a sealing mode, after stacking, fermentation and digesting, and the composite leaven is composed of bacillus subtilis, bacillus licheniformis, trichoderma harzianum and trichoderma viride according to the mass ratio (W/W) of 1.5:1:1:2.5:4. Auxiliary materials made of 5%-10% mushroom dregs and 6%-10% rice husk ash are evenly mixed with fermented and prepared main materials, arbuscular mycorrhizal fungi agent are added according to 600-1000g/T for compounding, and the microbial fertilizer is prepared after passing a 3mm sieve. Good production and efficiency improvement is obtained through application, and the microbial fertilizer has good application value.

A method of plant cultivation which comprises inoculating plants in a plant growing system comprising a container and an insert therefore, the microbial inoculant containing at least one species from the following group of microorganisms comprising arbuscular mycorrhizae associated bacteria, plant growth promoting rhizobacteria, yeast microorganisms and substrate conditioning bacteria.

The invention discloses a method for biologically restoring organic phosphorus pesticide polluted soil. An arbuscular mycorrhizal fungi and plant symbiotic system is used for accelerating the recovery of the organic phosphorus pesticide polluted soil. The method comprises the following steps of: (1) preparing an arbuscular mycorrhizal fungi agent; (2) inoculating the fungi agent to the soil to be restored, and planting quick growth plants, or transplanting the plants after mycorrhizal seedling culture; and (3) managing the plants to grow 2 to 3 months. The arbuscular mycorrhizal fungi agent comprises Glomus caledonium 90036, Acaulospora mellea ZZ, Glomus mosseae BEG167 and Glomus intraradices BEG 141). The host plant is selected from quick growth plants such as Sudan grass, alfalfa, corn, broomcorn and the like. The method can reduce the pesticide residue in agricultural products, reduce environment transfer to water, atmosphere and the like and reduce harm to human health, and is simple, convenient and environment-friendly.

The invention provides an application of arbuscular mycorrhizal fungi (AMF) in enhancement of growth and capability of heavy metal absorption from soil of black nightshade and a method of enhancing cadmium absorption of black nightshade from soil by utilization of the arbuscular mycorrhizal fungi. A cadmium (Cd) hyperaccumulating plant black nightshade is adopted as an experiment plant, the AMF is adopted as an Inoculant, the Cd hyperaccumulating plant black nightshade and the AMF capable of promoting plant growth are combined, and the AMF can enhance growth and capability of cadmium absorption from soil of the black nightshade, thus increasing the remediation efficiency of the black nightshade for Cd-contaminated soil. Compared with traditional chemical and agricultural measures, the method has characteristics of good treatment effects, no secondary pollution, low operation cost, and the like, and has good theoretical and application popularization value.

A method for inducing the drought resistance of cucumbers by arbuscular mycorrhizal fungi comprises the following steps: carrying out individual inoculation and propagation expanding initial arbuscular mycorrhizal fungi inoculants; cultivating cucumber seedlings through mycorrhization of arbuscular mycorrhizal fungi inoculants, wherein inoculation media adopts a mixture of a Hoagland nutrient solution and perlite; inducing drought resistance: after the seedlings are grown for 3 months, transferring the seedlings in black seedling raising bags with the specification of 25 cm*25 cm, filling 3.0 kg of sterilization soil in each black seedling raising bag, applying 80 mg/kg of total phosphorus and 100 mg/kg of total nitrogen in each basin, and keeping the relative water content of the soil to be about 40% of the field capacity under moderate drought; sufficiently watering before processing to enable the seedlings to be in the natural drought, carrying out the water control processing when the natural drought reaches the preset relative water content in processing, using a weighing water supplementing method to keep the all repeated soil water content in each-time processing to be within the test required range during the water control period, setting the regulating time of the water content to be 8: 00 Am each day, and supplementing water lost on a previous day.

The invention discloses a method for improving growth and drought-resistant ability of wheat by using arbuscular mycorrhizal fungi to bring the reciprocal symbiosis relation between AM fungi and host plants under drought stress into play reasonably and efficiently. According to the method, the AM fungi having a close symbiotic relation with the wheat are selectively used, and the symbiotic relation between the AM fungi and the wheat is built in a condition control mode to promote the wheat growth and improve drought adaptability. The AM fungi comprise one of glomus monosporum and acaulospora laevis, the two kinds of AM fungi are respectively propagated in an expanding mode in an expanding propagation matrix by using red clovers, and then two AM fungi inoculants and the wheat are cultured together in a symbiotic culture medium. The effective path for profitable symbiosis of the AM fungi and the host plants in adversity conditions is provided, the infection and symbiosis relation with the host plants of the AM fungi is improved in a man-made mode, and the yield of dry crops is improved.

The invention provides a method for locally culturing and producing arbuscular mycorrhiza fungus biological agent fertilizer in farmlands. The method is characterized by comprising the following steps of: 1, preparing a farmland culturing pond and a culture medium; 2, transplanting and culturing arbuscular mycorrhiza fungi; 3, absorbing and converting biological fertilizer; and 4, collecting biological fertilizer. Compared with the prior art, the technique adopted in the method has the advantages that: 1, the raw materials are readily available, the culture of the raw materials is easy, and the cost is low; 2, the culture technique and the production technique are conveniently popularized in rural areas; and 3, the production technique is green and environment friendly, which facilitates the development of modern agriculture. In addition, by using the fertilizer of the invention, the used amount of the quick-acting fertilizer is reduced and the used amount of partial fertilizer is reduced, so that the pollution to soil, water resources and air is lowered, and the fertilizer is particularly suitable to be used for planting seedlings of farm crops and medicinal plants. Tests show that the yield of the farm crops cultured by using the fertilizer of the method is improved by over 50 percent compared with that of the farm crops cultured by using the common fertilizer.

The invention belongs to the field of cultivation methods of tree peony and discloses the application method of AM (Arhusclar Mycorrhiza) fungi in the cultivation of tree peony. The application method comprises the following steps of: (1) material preparation; (2) substrate and holed plate sterilization; (3) inoculant preparation; (4) mycorrhiza seedling production; and (5) field transplanting and setting. When used in the cultivation of tree peony, the application method can improve the micro-ecological environment of tree peony rhizosphere, and especially has obvious alleviation effects on the aggravation of plant diseases and pest damages, untimely defoliation and other symptoms, which are caused by many-year continuous cropping, thereby achieving the dual purposes of improving the yield and the quantity of tree peony.

The invention discloses separation of arbuscular mycorrhizal fungi as well as preparation and application of arbuscular mycorrhizal fungi fungicide, and belongs to the technical field of biology. The arbuscular mycorrhizal fungi is arbuscular mycorrhizal fungi rhizophagus irregularis which is called arbuscular mycorrhizal fungi rhizophagus irregularis CD-1 and is separated from soils; a specific sequence of verifying ribosome 28S rDNA is shown in SEQ ID NO.1; by utilizing the fungicide prepared by the fungi, the height of strains, the number of petioles, the overground fresh weight, the overground dry weight, the root fresh weight and the root dry weight of tomatoes can be increased, and the mycorrhizal infection rate delta gt can be obviously prompted; 80 percent of corns are high in selfing line strains and heavy in overground fresh weight and overground dry weight; the resistance to banded sclerotial blight by the corns is improved, and the arbuscular mycorrhizal fungi has an extremely-high genetic use value.

The invention discloses a preparation of compound rice dedicated bio-fertilizer based on arbuscular mycorrhizal fungi and an application thereof, and relates to the preparation and the application of bio-fertilizer. The compound rice dedicated bio-fertilizer aims to solve the problem that rice can not be infected by the arbuscular mycorrhizal fungi under the aquatic condition at present and is difficult to effectively form mutualistic symbiosis, and therefore the rice can not obtain mineral nutrition from an arbuscular mycorrhizal fungi-crop symbiotic system. The method comprises the following steps of: preparing a colonized reinforcer; pre-processing arbuscular mycorrhizal fungi fungicide; and evenly mixing the processed arbuscular mycorrhizal fungi fungicide, rice shoots growth promoterand the colonized reinforce. The compound rice dedicated bio-fertilizer can be prepared into a seedling bed, culturing the mycorrhiza seedling and managing a rice field. According to the compound rice dedicated bio-fertilizer disclosed by the invention, the arbuscular mycorrhizal fungi-crop symbiotic system with a high infection rate and stable performance can be built in the seedling stage of the rice, and the symbiotic system can successfully live through the transplanted field flooding environment. The biological bactericide activity loss problem brought by flooding intimidation can be effectively solved, mineral nutrition is continuously provided for the rice, the growth situation of the rice is improved, the fertilizer application amount in the field is reduced, and the rice production cost can be effectively lowered.

The invention relates to a method for improving artificial breeding survival rate of populus diversifolia by inoculating arbuscular mycorrhizal fungi. The method comprises the steps of: selecting populus diversifolia sprouts for performing pot experiment, arranging a transplanting hole in the pot, spreading sandy soils rich in glomus mosseae spores at the bottom of the hole, slowly rotating the root systems of populus diversifolia seedlings on the sandy soils of glomus mosseae spores before the transplantation, so that the sandy soils are pasted on the root systems of the populus diversifolia seedlings, transplanting the populus diversifolia seedlings into the hole in the pot, watering a dilute Hoagland nutrient solution every time, and observing the survival rate; or enabling populus diversifolia seeds to absorb water and swell and accelerating germination in an incubator, mixing the populus diversifolia seeds with the sandy soils of glomus mosseae spores, spreading the mixture on a sandy layer surface of a culture bed, and paving a layer of sandy soils rich in glomus mosseae spores under the sandy layer of the culture bed in advance, wherein the root systems are to pass through the sandy soil layer rich in glomus mosseae spores, so that the root systems can be infected. Compared with a control group, the populus diversifolia seedlings obtained by the method are obviously different from the populus diversifolia seedlings in the control group in the growing speed and survival time, particularly in survival rate.

The invention relates to a method for treating garbage, sludge or excrement by earthworms and a biological organic fertilizer. The method comprises the following steps: adding a microbial starter into wastes to be treated and fermenting for one time to obtain organic materials which are fermented for one time; adding vesicular-arbuscular mycorrhizae into the organic materials which are fermented for one time and mixing to obtain a mixture; adding young earthworms into the mixture for cultivating; and drying a mixture obtained by cultivating until the humidity is 15%-20% to obtain the biological organic fertilizer, wherein 0.08-0.12 part by weight of the vesicular-arbuscular mycorrhizae is added into each 100 parts by weight of the organic materials which are fermented for one time; 30000-40000 young earthworms are added into each cube of the organic materials which are fermented for one time. According to the method for treating the garbage, the sludge or the excrement by the earthworms, the high-quality and efficient biological organic fertilizer is produced and a good environment is provided for the growth of plant roots.

The invention discloses a method for carrying out industrialized production on an arbuscular mycorrhizal fungi agent, and relates to a production method of arbuscular mycorrhizal fungi. The method comprises the following steps: 1. preparing and treating the arbuscular mycorrhizal fungi agent; 2. carrying out seed treatment on hosts; 3. carrying out breeding; and 4. managing. The method disclosed by the invention has the advantages that the implementation of production just needs eight weeks, the number of effective spores of arbuscular mycorrhizal fungi in each gram of the obtained arbuscular mycorrhizal fungi agent is 120-150, the production cycle is short, and the yield is high. The method disclosed by the invention can be applied to the field of agriculture, gardens, and flowers.

Mycorrhizal and rhizobial associations represent the two most important symbiotic relationships between higher plants and microorganisms, providing access to otherwise limiting supplies of phosphate and nitrogen, respectively. Although many higher plants are able to establish a symbiotic relationship with arbuscular mycorrhizal fungi, legumes are unusual among plants because they also form associations with nitrogen fixing soil bacteria called rhizobia. This symbiosis requires the production of bacterial signals, “Nod factors” that trigger several key developmental responses in the host plant (Dénarié et al., 1996). The DMI1 gene of the model legume M. truncatula plays a major role both in the early steps of Nod factor signaling and in the establishment of mycorrhizal symbiosis. Dmi1 mutants do not exhibit many of the early responses to Nod factors and are incapable of forming nitrogen fixing root nodules. Here we describe the cloning and preliminary characterization of DMI1. The DMI1 gene encodes a novel protein with low global similarity to ligand-gated cation channels of archaea. The protein is highly conserved in angiosperms and ancestral to land plants. Interestingly a putative A. thaliana DMI1 orthologous gene is expressed in roots. As A. thaliana is unable to establish a mycorrhizal symbiosis, this finding suggests that DMI1 may also exhibit a function that is independent of symbiotic interactions.

The invention discloses a Jatropha curcas mycorrhizal fungi compound fertilizer, which comprises one or more arbuscular mycorrhizal fungi containing glomus and gigaspora that are served as the mycorrhizal fungi. The compound fertilizer consists of 20-30 percent of the mycorrhizal fungi, 1-12 percent of phosphate fertilizer, 2-18 percent of potassium fertilizer, 8-15 percent of nitrogenous fertilizer, 2-3 percent of zinc sulfate and 20-50 percent of humus soil. The compound fertilizer of the invention can improve the absorption ratio of Jatropha curcas root absorbing soil nutrition, meet the growth, development and fruit bearing of the Jatropha curcas, improve soil ecological environment and increase seed yield.

The invention relates to a method for promoting growth of sweet sorghum in saline-alkali soil through arbuscular mycorrhizal fungi. The method includes the steps of preparing and applying an arbuscular mycorrhizal fungi agent. When the arbuscular mycorrhizal fungi agent is prepared, a mixture of farmland soil and organic fertilizer serves as a substrate, and the organic fertilizer and decomposed products of the organic fertilizer can be used as a fungi protecting agent to have a buffering effect on saline ions in soil around the arbuscular mycorrhizal fungi in the early period when the fungi agent is applied to the saline-alkali soil so as to help the arbuscular mycorrhizal fungi to resist saline-alkali stress; the symbiotic relationship is smoothly formed between the arbuscular mycorrhizal fungi and the sweet sorghum, the mineral nutrition of the sweet sorghum is remarkably improved, the sweet sorghum can be helped to resist saline-alkali stress, and the growth of the sweet sorghum is promoted.

The invention discloses a new making method of mycorrhiza bacterial agent, which is characterized by the following: allocating raw material of sand, zeolite and pearlite with weight rate at 10:10:1; culturing; accelerating mycorrhiza infecting rate and overground dry weight; selecting the zeolite with density at 1.0g/ml, pearlite with density at 0.1g/ml and sand with density at 1.5g/ml; fitting for distant transportation.