Repair method for cadmium polluted soil by arbuscular mycorrhiza-alfalfa symbiont

A technology of cadmium-contaminated soil and arbuscular mycorrhiza, applied in the field of remediation of arbuscular mycorrhizal-alfalfa symbionts to cadmium-contaminated soil, can solve the problems that the soil effect cannot be maximized, the cost is high, and the effective time is long. , to achieve good theory and application promotion value, good comprehensive ecological benefits, and low operating costs

Inactive Publication Date: 2018-09-21
SOUTHWEAT UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The complexity and difficulty of remediation of cadmium-contaminated soil means that there is currently no truly stable and efficient remediation technology that can meet the needs of actual production; physical remediation and chemical remediation can quickly reduce the cadmium content in soil, but they only change The existing form of cadmium in the soil has not been completely removed, and there are often problems of high cost, huge engineering volume, and secondary environmental pollution; animal remediation and microbial remediation, as a green remediation technology, are economical, It is convenient and does not change the inherent physical and chemical properties of the soil, but its slow repair speed, long effective time, and high requirements on the soil environment limit its large-scale application
The use of plants to repair the environment polluted by cadmium is not only low cost, but also has good comprehensive ecological benefits, especially suitable for large-scale promotion, but its effect on cadmium-contaminated soil cannot be maximized

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A method for repairing cadmium-contaminated soil by arbuscular mycorrhiza-alfalfa symbiosis, comprising the following steps:

[0025] Step 1. Mix the arbuscular mycorrhizal fungus with the sterilized propagation substrate evenly according to the inoculation amount of 5% by mass, and then fill the mixed substrate into 2 / 3 of the sterile pot; after sowing the Sudan grass seeds, cover them again A layer of 0.5cm sterilized sandy soil culture medium was cultivated in a greenhouse; after 3 months of pot cultivation, all the stems and leaves of the aboveground part of the Sudan grass plant were cut off, and the pot was placed in the room to dry for 1 week, and all the cultures in the pot were harvested; Pour all the cultures on sterile paper, chop them up with sterilized tools, transfer them into sterile bags, seal the bags and refrigerate them to obtain the arbuscular mycorrhizal fungal agent; all the cultures mentioned include plant roots , mycelia, spores, sand medium and ...

Embodiment 2

[0029] A method for repairing cadmium-contaminated soil by arbuscular mycorrhiza-alfalfa symbiosis, comprising the following steps:

[0030] Step 1. Mix the arbuscular mycorrhizal fungus with the sterilized propagation substrate evenly according to the inoculation amount of 8% by mass, and then fill the mixed substrate into 4 / 5 of the sterile pot; after sowing the Sudan grass seeds, cover One layer of 0.5cm sterilized sand medium, cultured in the greenhouse; after 4 months of potted culture, cut off all the stems and leaves of the aboveground part of the Sudan grass plant, place the pot in the room to dry for 2 weeks, and harvest all the cultures in the pot; Pour all the cultures on sterile paper, chop them up with sterilized tools, transfer them into sterile bags, seal the bags and refrigerate them to obtain the arbuscular mycorrhizal fungal agent; all the cultures mentioned include plant roots , mycelia, spores, sandy soil culture medium and propagation substrate, and the pr...

Embodiment 3

[0034] In the second step, it also includes: adding the treated alfalfa seeds into the seed soaking liquid and soaking them for 2 hours; then taking them out to dry naturally, and then applying a pulse electric field with an electric field strength of 60kV / cm, a pulse width of 12μs, and a pulse frequency of 100Hz. During the soaking process, ozone was passed into the seed soaking solution, and the ventilation rate of ozone was 100L / min;

[0035] The seed soaking liquid comprises the following raw materials mixed by weight: 0.3 parts of gibberellin, 2 parts of potassium selenite, 0.5 part of sodium laurylalanine, 0.5 part of sodium naphthenate, sodium humate 0.3 parts, 2 parts of hydroxyethyl ethylenediamine triacetic acid, 0.8 parts of alkyl glucoside, 0.5 parts of nano-zinc oxide, 0.8 parts of purple sweet potato anthocyanin, 1 part of sodium alginate, 1 part of spirulina extract, onion extract 1 part of food, 300 parts of rice washing water.

[0036] All the other process p...

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PUM

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Abstract

The invention discloses a repair method for cadmium polluted soil by an arbuscular mycorrhiza-alfalfa symbiont. The repair method comprises the following steps: S1, preparing an arbuscular mycorrhizafungi fungicide; S2, treating alfalfa seeds; and S3, inoculating the arbuscular mycorrhiza fungi fungicide to the surface sol layer of the cadmium polluted soil; then planting the alfalfa seeds for field management; and after growth, harvesting plant materials, airing and transferring the plant materials, and incinerating the plant materials in a centralized manner to repair the cadmium polluted soil. By combining a microbial repair technology with a plant repair technology, the repair method solves the problem that the repair method is prevented from being popularized and applied on a large scale as a green repair technology because the microbial repair speed is slow, the responding time is long and the soil environmental requirement is high, is low in cost by plant repair, good in comprehensive ecological benefit, suitable for being popularize on a large scale and the like, and has the characteristics of being economical and convenient to repair microbially, not changing the inherentphysical and chemical properties of soil and the like. The obtained repair method is high in treatment capacity, economic, convenient and suitable for being popularized on a large scale.

Description

technical field [0001] The invention relates to a method for repairing cadmium-contaminated soil, in particular to a method for repairing cadmium-contaminated soil by arbuscular mycorrhiza-alfalfa symbionts. Background technique [0002] At present, the problem of my country's cultivated land being polluted by heavy metals such as cadmium, arsenic, and lead is becoming more and more serious, and the resulting economic losses are also increasing. It is reported that the economic losses caused by heavy metal pollution in my country are at least 20 billion yuan every year. Among all heavy metal pollution, cadmium pollution is the most serious. In recent years, with the development of industries such as mining, metallurgy, and cadmium treatment, agricultural soil in my country has become increasingly polluted by cadmium. Since cadmium cannot be degraded by microorganisms in the soil, its half-life exceeds 20 years, and its pollution is an irreversible accumulation process; cadm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B09C1/10B09C1/00C09K17/40
CPCB09C1/10B09C1/105B09C2101/00C09K17/40Y02P20/54
Inventor 竹文坤段涛石培珩周建姜磊
Owner SOUTHWEAT UNIV OF SCI & TECH
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