Repair method for cadmium polluted soil by arbuscular mycorrhiza-alfalfa symbiont
A technology of cadmium-contaminated soil and arbuscular mycorrhiza, applied in the field of remediation of arbuscular mycorrhizal-alfalfa symbionts to cadmium-contaminated soil, can solve the problems that the soil effect cannot be maximized, the cost is high, and the effective time is long. , to achieve good theory and application promotion value, good comprehensive ecological benefits, and low operating costs
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Embodiment 1
[0024] A method for repairing cadmium-contaminated soil by arbuscular mycorrhiza-alfalfa symbiosis, comprising the following steps:
[0025] Step 1. Mix the arbuscular mycorrhizal fungus with the sterilized propagation substrate evenly according to the inoculation amount of 5% by mass, and then fill the mixed substrate into 2 / 3 of the sterile pot; after sowing the Sudan grass seeds, cover them again A layer of 0.5cm sterilized sandy soil culture medium was cultivated in a greenhouse; after 3 months of pot cultivation, all the stems and leaves of the aboveground part of the Sudan grass plant were cut off, and the pot was placed in the room to dry for 1 week, and all the cultures in the pot were harvested; Pour all the cultures on sterile paper, chop them up with sterilized tools, transfer them into sterile bags, seal the bags and refrigerate them to obtain the arbuscular mycorrhizal fungal agent; all the cultures mentioned include plant roots , mycelia, spores, sand medium and ...
Embodiment 2
[0029] A method for repairing cadmium-contaminated soil by arbuscular mycorrhiza-alfalfa symbiosis, comprising the following steps:
[0030] Step 1. Mix the arbuscular mycorrhizal fungus with the sterilized propagation substrate evenly according to the inoculation amount of 8% by mass, and then fill the mixed substrate into 4 / 5 of the sterile pot; after sowing the Sudan grass seeds, cover One layer of 0.5cm sterilized sand medium, cultured in the greenhouse; after 4 months of potted culture, cut off all the stems and leaves of the aboveground part of the Sudan grass plant, place the pot in the room to dry for 2 weeks, and harvest all the cultures in the pot; Pour all the cultures on sterile paper, chop them up with sterilized tools, transfer them into sterile bags, seal the bags and refrigerate them to obtain the arbuscular mycorrhizal fungal agent; all the cultures mentioned include plant roots , mycelia, spores, sandy soil culture medium and propagation substrate, and the pr...
Embodiment 3
[0034] In the second step, it also includes: adding the treated alfalfa seeds into the seed soaking liquid and soaking them for 2 hours; then taking them out to dry naturally, and then applying a pulse electric field with an electric field strength of 60kV / cm, a pulse width of 12μs, and a pulse frequency of 100Hz. During the soaking process, ozone was passed into the seed soaking solution, and the ventilation rate of ozone was 100L / min;
[0035] The seed soaking liquid comprises the following raw materials mixed by weight: 0.3 parts of gibberellin, 2 parts of potassium selenite, 0.5 part of sodium laurylalanine, 0.5 part of sodium naphthenate, sodium humate 0.3 parts, 2 parts of hydroxyethyl ethylenediamine triacetic acid, 0.8 parts of alkyl glucoside, 0.5 parts of nano-zinc oxide, 0.8 parts of purple sweet potato anthocyanin, 1 part of sodium alginate, 1 part of spirulina extract, onion extract 1 part of food, 300 parts of rice washing water.
[0036] All the other process p...
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