Preparation of compound rice dedicated bio-fertilizer based on arbuscular mycorrhizal fungi and application thereof
A technology of arbuscular mycorrhizal fungi and biological fertilizers, which is applied in the application, rice cultivation, fertilizer mixture and other directions, can solve the problems such as the inability of rice to obtain mineral nutrition, difficult symbiosis, and arbuscular mycorrhizal fungi infestation, etc., so as to improve the quality of rice. The effect of distribution, high rate of smearing, and good ecological environment
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specific Embodiment approach 1
[0013] Specific embodiment one: present embodiment is based on the preparation of the composite rice special-purpose biological fertilizer of arbuscular mycorrhizal fungus, according to the following steps: one, the preparation of colonization reinforcement: 1kg white clover seed adds 1.5kg water and 0.001kg molybdenum Ammonium acid, soaked for 12 hours, rinsed with distilled water for 3 to 5 times, and obtained after drying;
[0014] 2. Pretreatment of the arbuscular mycorrhizal fungal agent: cut the white clover roots in the arbuscular mycorrhizal fungal agent to 1~2cm, crush the matrix in the arbuscular mycorrhizal fungal agent and pass through a 10-mesh soil sieve , and the treated arbuscular mycorrhizal fungus inoculum is obtained after the two are mixed evenly;
[0015] 3. Mix the treated arbuscular mycorrhizal fungal agent, rice seedling strengthening agent and colonization enhancer according to the volume ratio of (1500~2500):(300~600):1, and then complete the arbuscul...
specific Embodiment approach 2
[0018] Specific embodiment two: what this embodiment is different from specific embodiment one is the preparation of the arbuscular mycorrhizal fungus inoculum in the step 3, carry out according to the following steps: one, field soil, river sand and vermiculite cross 10 mesh soil sieves respectively , and then mix field soil, river sand and vermiculite in a volume ratio of 2:5:3 as a substrate, sterilize the substrate by high-pressure steam at 121°C for 2 hours, wash and dry the flower pots, and then use 0.1% high manganese Potassium acid potassium solution for 30 minutes; 2. Soak white clover seeds in alcohol with a volume concentration of 75% for 10 minutes, then rinse 3 times with distilled water, and accelerate germination at 28°C; 3. Put 6 kg of sterilized Substrate, spray sterile water until the water seeps from the base of the pot, inoculate the substrate with arbuscular mycorrhizal fungi at an inoculation rate of 20 spores per pot, and then evenly sprinkle with germina...
specific Embodiment approach 3
[0020] Specific embodiment three: the difference between this embodiment and specific embodiment one is that the processed arbuscular mycorrhizal fungal agent, rice seedling strengthening agent and colonization enhancer are mixed in a volume ratio of 2000:450:1 in step 3 . Other steps and parameters are the same as those in Embodiment 1.
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